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A critical evaluation of methods used for S-genotyping: from trees to DNA level
19-29.Views:419Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.
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Detection and identification of phytoplasmas in peach based on woody indexing and molecular methods
35-41.Views:277Symptoms resembling phytoplasma disease have been observed on peach trees in a seed-source plantation of stone fruits in south Hungary quite recently. In this publication we report on the results of woody indexing of symptomatic peach trees on GF 305 indicator in the field and under greenhouse conditions as well as on molecular studies. Phytoplasma infection detected on GF 305 indicators in greenhouse and field indexing was confirmed by PCR. Nested PCR was conducted using universal primer pairs followed by group and subgroup specific primers for the second amplification. RFLP analysis of nested PCR products was performed using Rsal restriction enzyme. Based on the results of molecular studies it can be concluded that phytoplasmas, belonging to the European stone fruit yellows subgroup (16SrX-B) were identified in peach trees. Further studies on symptomatic peach trees originating from different parts of Hungary are in progress.
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Vineyards characteristic by using GIS and refl ectance measurements on the Nagy-Eged hill in Hungary
57-60.Views:444Our investigation was carried out in vineyards on Nagy-Eged hill in Hungary. Grapevine growing plays an important role in Eger Vine Regions. In the grapevine cultivation it becomes necessary to know the requirements of vineyards along the best geographical situation using available cultivation and examination technology to achieve better and quality produce. This study reviews applicability of the different spatial analyst techniques in vineyard especially models based on surface relief and the new technology of hyperspectral analysis. The spectral alteration was examined between different health status and leaves infected by Eriophyes vitis to defi ne spectral characteristics. Thus reflectance measurements could also support to evaluate airborne hyperspectral images with the help of the spectral library.
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Chemical analysis of the pollen of plum varieties
25-26.Views:1056The chemical composition, amino acids and carbohydrates, of the pollen has been analysed in varieties grown in Hungary during the years 1993 at Kecskemet and 1995 at Cegléd and Pomáz. The distinction of 17 amino acids and 7 carbohydrates has been achieved. The aim of the study was to find criteria for the distinction of varieties.
The total content of amino acids varied between 15.53 and 20.54 %. The highest ratios represented the asparagine and the glutamine acids. The relative content in other amino acids was between 2 and 7 % of the total. The less frequent were cysteine and valine.
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Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
75-79.Views:254Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.
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Effects of wastewater irrigation on soil physico-chemical properties and vegetables quality: A review
7-16.Views:699This study analyses the use of raw urban wastewater for irrigation and its effects on soil characteristics and heavy metal pollution within crops. There is rampant use of urban waste water by small scale urban vegetable farmers in Zimbabwe. This is posing health hazards to the consumers of urban vegetables and decrease in soil functioning. The significance of this study was to appraise the research study related to the use of urban waste water to answer the research question: What is the impact of waste water on selected soil physico-chemical properties and quantities of heavy metals in vegetables irrigated with waste water? A systematic review of 3100 articles from PubMed, Scopus, Web of Science, and Google Scholar (2010–2024) was performed within PRISMA guidelines. After excluding irrelevant studies, 49 from Nigeria, Bangladesh, Iran, Egypt, India, Pakistan, China, Saudi Arabia, and DRC were selected for analysis. These countries were found to have a lot of literature on urban waste water irrigation. This literature review identified an overview of existing literature on urban waste water irrigation and synthesise findings to provide a comprehensive overview of the topic The results showed (P<0.05) soil properties changed significantly: pH (6.0–7.0 to 4.5–5.5), electrical conductivity (0.2–0.4 to 1.5–2.0 dS/m), organic matter (2–3% to 4–6%), cation exchange capacity (10–15 to 20–25 meq/100g) all aligning with the intended outcomes. The level of nutrients (N, P, K) shifted alongside the source of wastewater. Soil also accumulated heavy metals (Zn, Cd, Pb, Fe, Mn, Cu) while the crops accumulated unsafe levels of Pb (2.5 mg/kg), Cd (1.2 mg/kg), and Cr (3.1 mg/kg) which surpassed WHO/FAO limits. The results highlighted the concern for health and environmental hazards. Treatment of the wastewater, monitoring of the soil, and stricter guidelines are needed for safe agricultural practice.
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The comparative economic analysis of Hungarian and German apple production of good standard
79-85.Views:546The profitability of the Hungarian apple production considering firms producing on high standard is not lagged behind significantly from that of German firms, moreover in certain cases it reflects a more positive situation. It is unfavourable, however, that this statement is true only for 8 to 10% of our whole apple plantation surface. The results of the investigations highlighted the fact that in comparison with Germany our farm business advantages manifest in three factors: in 70 to 80% lower wages, in 15 to 30% higher investment and subsidy intensity and in the fact that at present we cannot neglect the ice safety system which is rather expensive. By the increasing wages, the narrowing subsidy opportunities and incidentally the appearing harmful weather phenomenon, these advantages may be continuously ceased. Our definite disadvantage appears in the level of marketing price, considering the fact that producers in Hungary realize 30 to 35% lower marketing price, which is in connection with the probably much lower level of organization among farmers, in the market and in the logistical background.
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An investigation of genotype-environment interaction and stability for pea (Pisum sativum L) seed yield
53-55.Views:212Eighteen pea breeding lines and three check cultivars were tested in two years with and without irrigation. The linear regression can explain only a small part of GEI so the use of regression technique was not possible for interpreting the data. In this case the ecovalence, stability variance and superiority measure stability parameters cannot describe properly the genotype's response. With the AMMI method it is possible to group properly the genotypes according to their response.
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Character association and genetic gain of nine agronomic traits of F1 populations in onion (Allium cepa L.)
50-58.Views:694F1 populations of half diallel cross were considered for this investigation. Phenotypic component of variation (s2P) was higher than genotypic (s2G), interaction ((s2I) and within error (s2w) components of variation. Trait bulb weight showed the highest values for s2P, s2G, s2I and s2w. The noticeable amount of phenotypic, genotypic, interaction and within error covariation was found in the combination of BW×BY, possibly indicating wide scope of selection for this pair of characters. Genotypic correlations were higher than the respective phenotypic correlations. This situation was also marked in the path coefficient analysis. Bulb yield/plot showed highly significant and positive correlation coefficient with other characters both at phenotypic and genotypic levels. When all the nine characters were included in an index, it exhibited the highest genetic gain as percentage. When a combination of two or more characters was studied in a function, the efficacy was higher than that of direct selection for bulb yield. The combination of five, six, seven or eight characters showed higher percentage of expected gain. Due to significantly correlated with BY and having high positive direct effect at phenotypic level characters viz., LL, BW, PH and NLs is considered as primary yield components. Again combinations of these four characters gave the commendable expected genetic gain of 330.7290% may be considered as important selection index for this material.
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Colour and water content detection of sweet cherry by portable spectrometer
23-26.Views:35456Based on the most recent data, the average amount of sweet cherry produced in Hungary is around 10-12 thousand tons. Therefore fast and effective method is important for sweet cherry fruit quality analyses. The aim of the study was to examine the applicability of reflectance measurements for sweet cherry fruit quality analyses. In our experiment five cherry species (Vera, Cristalina, Germersdorfi, Noir de Mechet, Canada Giant) were examined in order to measure the spectral differences between species. Further more, spectral alteration was examined between different health and maturity status of the fruits in the case of a specified, the Germesdorfi species. The four new indices are appropriate tools for cherry quality analysis. Thus reflectance measurements can also support more precise and automated fruit selections. The methods for the differentiation of species could also be viable at a concerned habitat; however, the climate, habitat and soil conditions strongly affect the yield quality. Concerning the fast determination of water content, WBI could be a reliable method for the assessment
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Interaction of nutrient supply and crop load of apple trees (Malus domestica Borkh.)
33-35.Views:307Long term fertilisation trials were combined with storage experiments with 'Jonathan' apple trees and fruits to study influence of tree nutrition on quantity and quality of crop. The site of experiments is a typical Carpathian-basin environment with loamy silt soil, high lime content and arid summers. Conclusions has been drown from six years' set of data. Augmented levels of soil fertilisation increased cropping capacity of apple trees, however, the fruit load has not met with cropping capacity in every year. More the def cit came into view in crop load, less the fruit quality resulted in. The deficit in cropping capacity, however, could not have been determined with simple rates as fruit weight per trunk circumference or similar. Better determination was obtained where foliar nutrient contents were correlated to crop per tree figures. In general terms, the N and Ca content in leaves increased with yields when K and P content formulated reciprocally. When storage quality of 'Jonathan' apple fruits were related to crop load (kg/tree), influence of crop deficit became visible. As the crop load and foliar nutrient levels interacted, the fruit quality (number of disordered apples after 6 month of storage) subjected of both physiological phenomena. Higher determination degree were obtained when crop load was assessed together with single or multiple foliar analysis data.
