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Production of transgenic bean callus via genetic transformation by DNA-coated tungsten particles
43-47.Views:173Callus cultures were induced from hypocotyl of young bean seedlings. The B5 medium completed with 1 mg/1 KIN and 2mg/1 2,4-D proved the best. Callus developed and maintenaned on B5 medium supplemented with 1mg/1 kinetin and 2mg/I 2,4-D. The B5 medium supplemented with 1mg/1 KIN and 2mg/1 2,4-D induced much more callus than half strength MS medium supplemented with 0.5 or 0.75mg/1 BA and 0.1 mg/1 NAA. The results demonstrate that GeneboosterTM is convenient method to obtain transient gene expression in callus of bean. The results have shown that the bean callus shot by GeneboosterTM can be transformed to get (kanamycin-resistant and stress mannitoltolerant) calli. The presence of mannitol-dehydrogenase gene (mt/) was verified by PCR, showing the integration of mt/ gene carried by two plasmids. Co-transformed calli were selected after bombardment on kanamycin, mannitol and (kanamycin+mannitop-containing media. Data of molecular analysis (PCR) confirmed the insertion of mtl gene in the genome of mannitol-tolerant callus lines.
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Effect of the terroir and the harvest time on the Kékfrankos grape berries visual and physical parameters in Eger (Hungary)
81-85.Views:234In this research, the effects of the “terroir” and the harvest time on the most important physical properties of the berries of Kékfrankos (Blaufrankish) grape variety were examined in 2010 and 2011. The results of the experimentation shown, that the maturation processes can be well described by different physical parameters of the grape berry. The terroir and harvest time effects were investigated in two different vintages and four different territories. It can be concluded, that these physical parameters change significantly during the ripening period, but trends differ between vintages and regions.
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Climate change impacts and product lines
79-83.Views:461This paper summarizes the main effects of extreme weather events on agricultural production and demonstrates their economic consequences. For cost-benefit analysis of economic impacts and for determination of risk levels simulation models are needed that contains the relationship between product line levels and elements. WIN-SIM model is developed for this goal, specialized for wine production. The model is suitable to analyze the market share, the cost and income relations as well as the relation structure of the product lines. The four levels of the model (site, vine growing, wine production and wine market levels) have individual values added from the aspect of end product, where the product line sets out from the site level and gets through the levels up to the consumer segments. Theoretically, all elements can be connected to any element of the next level and sublevel, but there are “prohibited contacts” because of professional, regulation or production practice reasons.
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Microsatellite based identification of grapevine cultivars traditional in Hungary and in the Carpathian Basin
71-73.Views:242Grapevine cultivars and clones traditional in Hungary and in the Carpathian Basin are maintained in the genetic collection of the Corvinus University of Budapest. Mostly ancient varieties and clones were genotyped using microsatellite loci. The investigated 6 loci were sufficient to distinguish all cultivars. Some clones could also be separated based mostly on the variable VVS2 loci. For 17 out of the 31 investigated cultivars this is the first report on characterization of the polymorphism of the allele lengths by microsatellite markers on loci: VVS2, VVMD7, VVMD27, vrZAG62.
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Effect of seedling quality on growth, yield and quality of tomato (Solanum lycopersicum L.)
64-72.Views:518A two trial greenhouse experiment was carried out at Rwanda-Israel Horticulture Centre of Excellence located at Mulindi Station to evaluate seedling quality on growth, yield and quality of tomato. The seedlings were grown in different growing media and produced seedlings with varying quality indices. The growing media of peat moss 100% (T2) and sand + goat manure + carbonized rice husks 50%: 10%: 40% (T8) were revealed in seedlings with the highest mean quality indices of 31 and 28 respectively, while sand 100% (T2) presented the lowest quality indices during both trials. The transplants were planted in polybags filled with 2:1 of topsoil and kitchen manure arranged in a randomized complete block design (RCBD) with four replications. Collected data were subjected to analysis of variance and means were separated using HSD test at a 5% level of significance. The results revealed that the seedlings grown in T1 (S1) and T8 (S8) consistently presented tomatoes with better growth performance and yield. S1 and S8 produced mean yield of 93.59 and 92.35 t/ha respectively while S2 had the lowest yield with 53.86 t/ha. The fruit produced from seedlings grown in T4 (S4) had the highest mean sugar acid ratio of 5.88 but not significantly different from 5.61 and 5.44 of S1 and S8 respectively. Hence, there was a positive relationship among seedling quality and growth and yield performance of tomato but not in fruit quality.
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Metaxenia in apples cv. 'Rewena', 'Relinda', 'Baujade' as influenced by scab resistant pollinizers
11-14.Views:552Fruit quality of cross pollinated apples (Malus x domestica) influenced by the metaxenic pollen effect of the pollinizer was observed in Hungary. Flowers of three resistant cultivars (`Baujade', `Rewena') were hand pollinated with other resistant apple cultivars. Fruits were harvested on 25 September, 2005. Fruit quality was investigated in the laboratory of the Department of Pomology; Corvinus University of Budapest. Not only size and morphological parameters (diameter, height, stem length), but also refraction and acidic content of the fruits were measured.
According to the statistical analysis significant differences were determined on fruits among the groups as an effect of the pollen provider. In consideration of size parameters (diameter, height, weight) of `Rewena' fruits pollination partner 'Freedom' and 'Prima' caused outstanding results but `Florina' caused flatter fruits. Pollen of `Florina' and `Freedor-,' caused a higher percent refraction in the fruits of `Rewena'. In the case of `Baujade' fruits `Reglindis' — among cultivars we used as pollinizer — caused the biggest fruits medium flesh firmness and harmonic inner content values. `Rajka' caused on one hand smaller fruits and on the other hand higher flesh firmness and inner content values in the case of `Relinda' fruits. According to our data measured pollinizers varied the stem length as well.
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Effect of acidity on growth rate and stroma formation of Monilia fructigena and M. polystroma isolates
63-67.Views:374The effect of acidity (pH) ranges on the mycelial growth and stroma formation of Monilia fructigena Pers: Fr. and of M. polystroma van Leeuwen was determined on agar plates and apple fruits. Four isolates of each of the brown rot fungi and two apple cultivars, `James Grieve' and 'Cox's Orange Pippin', were used for the study. For the agar plate study, a range of the initial pH was prepared from 2.5 to 6.5. The dishes were inoculated with a 4 mm plug of each isolate and incubated at 23 °C in darkness. The mycelial growth was measured after 1.5, 4, 7, 10 and 20 days of incubation. After a 30-day incubation, stroma formation was determined by image analysis and weighing of mature stroma. In the fruit experiment, both cultivars were inoculated with one isolate of M. fructigena and of M. polystroma. The pH changes were determined after 7, 14, 28 and 35 days of incubation in both healthy and inoculated fruits. The fastest mycelial growth was at pH 4.5 for M. polystroma and at pH 3.5 for M. fructigena. After a 30-day incubation, M. polystroma isolates produced twice or three times more stroma compared to M. fructigena isolates. For both brown rot fungi, the amount of mature stroma increased from pH 3.5 to 5.5, and then decreased at pH 6.5. Results of the.fruit experiment showed that healthy fruits were quite acidic (pH < 3.5), but pH rapidly increased in the inoculated fruits for both cultivars, reaching pH 4.6-5.4 depending on cultivar and fungus isolate. On both cultivars, the stroma developed at a significantly higher pH for M. polystroma than for M. fructigena. Biological and practical implications of the results are discussed.
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Comparative analysis of apricot cultivars based on their ecological and biological indicators
35-50.Views:404The herbaceous plants organic characterize Ellenberg et al. worked out (1991), well-use system, which is updated with herbaceous and woody plant in the Hungarian flora species, so Soó (1964-1985), Zólyomi et al. (1967), Précsényi (1986) and Simon (1988) also addressed by different aspects of this problem circuits. The author is the first extended-Borhidi –Ellenberg’s system of wild fruit species (Surányi 2000, 2006) and cultivated of fruit (Surányi 2014) as well. Additional considerations there were aspects of the study of fruit varieties, these biological indicators following open pollination, frost tolerance, resistance of Sharka virus and disease susceptibility for. Firstly, we introduced a system for improving it a plum species and cultivars (Surányi 2015). In this case we used the new system among species and varieties of apricots, because diversity was able to express significantly. Especially the SB, WB, NB, and the relative biological value figures showed the variety. RB (reaction figures) fluctuated only slightly among the 463 varieties, but the dynamic difference between the 11’s was an indicator for the characterization of apricots. If the comparison performed plum and apricot variety’s level anyway justified the use of 11 kinds of organic and biological indicators.
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Evaluation of sour cherry varieties grown with environmental technology
39-41.Views:1922The evaluation of a produce is an important moment of predicting its success on the market. The general impression, which is decisive, when a consumer chooses to purchase the commodity, should be interpreted in more objective, measurable terms. Primary data have been collected on fresh fruits derived from different growing technologies by organoleptic tests and the data filled up in the forms are processed with correlation analysis. The components were: taste, aroma, flesh firmness. The coefficients of correlation showed that the rest of characters as the appearance, juiciness, and the sugar/acid ratio.
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Ecological diversity of Hungarian medicinal and aromatic plant flora and its regional consequences
10-19.Views:302During the last century the medicinal and aromatic plant sector has became a successful part of the Hungarian Agriculture. Some of the national products have been accepted as a special Hungarian ones ("Hungaricum"), evaluated on the world market, respectfully. By the estimates the cultivation area of medicinal and aromatic plants increased up to 37,000-42,000 hectares and considerable amount — about 10, 000-15, 000 tonnes of dry biomass — are produced by utilisation of Hungarian indigenous flora, year by year.
In the present work ecological requirements of 97 collected and 55 cultivated medicinal and aromatic plants are characterised. Based on the analysis of -Ts (temperature regime values) about 63 per cent of cultivated species came from Submediterranean and Mediterranean type of habitat, originally, while the majority of collected plants (61.8 per cent of them) prefer the deciduous forest conditions. The differences between collected and cultivated species are appreciable too, if the distributions of their characteristic water regime ('W' values) are compared. The majority of cultivated species require dry (moderate dry) and fresh (moderate fresh) habitats, while the amplitude of water requirement of collected species is much more wide-ranging.
The regional specialisation of Hungary according to production of medicinal and aromatic plants is known from the beginnings of the 20th century. As a result of spontaneous process seven well-defined production areas were developed. The relationship between regions, their climatic conditions and spectrum of species produced there are analysed.
