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Eurázsiai fajtákra alapozott borszőlő nemesítési program és eredményei
31-35.Views:215Eurázsiai fajtákra alapozott borszőlő nemesítési program és eredményei
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Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR
21-30.Views:478Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and sequencing are also briefly reviewed.
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Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material
69-80.Views:490Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.
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Certification programme for production of virus-free propagating material of grapevine and its results in Hungary
39-43.Views:277In Hungary, detection of virus and virus-like diseases of grapevine began in 1960's at the Research Institute for Viticulture and Enology by János Lehoczky and his colleagues. At present, sixteen virus and virus-like diseases of Vitis vinifera are known to occur in Hungary.
Regular virological screening of grapevine varieties started in 1972. The present system of screening (visual selection, indexing, ELISA) has been established using methods with continuous improvement according to recommendations of international organizations.
In the first year symptomless grapevine plants are selected and marked during surveys carried out twice in the vegetation period: at about flowering and in the second half of September. At the first selection time plants are sampled for ELISA.
In the spring of the second year, overwintered canes are checked by woody indexing on 8 indicator species in the field.
In the third and fourth years the nursery is evaluated twice again. At the end, the marked grapevine plants, giving negative results on all indicators in every case, are considered virus-free.
In autumn of the fourth year, the virus-free material is planted out under screenhouse and also in a special mother block (nuclear stock) for maintenance and propagation.
Mother blocks of virus-free scion varieties have been established on 2 ha and those of rootstock varieties on 0.5 ha planted with the following number of varieties included in the national list: 71 European scion — and 12 rootstock varieties or variety candidates/clones. It is necessary to increase the area of Pre-base, Base and Certified stocks exclusively with tested virus-free (clean) material.
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Vineyards characteristic by using GIS and refl ectance measurements on the Nagy-Eged hill in Hungary
57-60.Views:446Our investigation was carried out in vineyards on Nagy-Eged hill in Hungary. Grapevine growing plays an important role in Eger Vine Regions. In the grapevine cultivation it becomes necessary to know the requirements of vineyards along the best geographical situation using available cultivation and examination technology to achieve better and quality produce. This study reviews applicability of the different spatial analyst techniques in vineyard especially models based on surface relief and the new technology of hyperspectral analysis. The spectral alteration was examined between different health status and leaves infected by Eriophyes vitis to defi ne spectral characteristics. Thus reflectance measurements could also support to evaluate airborne hyperspectral images with the help of the spectral library.
