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• Recent developments in biochemical characterization of Vitis vinifera L. varieties in Hungary

  57-61.

  É. Stefanovits-Bányai

  S. Lakatos

  M. Hajós-Novák

  E. Hajdu

  I. Balogh

  Views:

  179

  Isoelectric focusing is an effective and well reproducible method to provide information for identification of various plant species and clones if breeding or other genetic modification(s) for a given species are reflected in changes of an isozyme pattern. The method has been used for characterization of plant proteins and enzymes and for identification of various species and varieties. Our aim was to continue our several-year-work carried out on a wide variety of grapevine varieties and to reveal whether analyses of esterase and peroxidase isozyme patterns are suitable to distinguish various grapevine varieties. Therefore we compared esterase and peroxidase isozyme patterns of various species. Plant samples were obtained from Szigetcsep, Kecskemet, Tokaj and Eger. The following samples were analyzed: Pinot gris, noir, blanc, Chardonnay, Riesling Theses, Chasselas from Szigetcs6p, Bianca and his parents Eger2 and Bouvier from Kecskemet, Furmint and Hárslevelű from Tokaj, Kékfrankos and Zweigelt from Eger. To identify various species according to their esterase isozyme patterns the after blooming phenological phase while according to their peroxidase isozyme pattern the dormant phenological phase was found as optimal sampling time.

• Production data of wine grape gene bank (Vitis spp.) of University of Debrecen, east Hungary

  32-36.

  N. Rakonczás

  Views:

  227

  National wine strategy of Hungary promotes the use of “flexible” grape cultivars. These enable producers’ best fit to wine market changes and expectations. This study is aimed to present data on the gene bank of the University of Debrecen, Hungary. Data were collected at a single site, between 2010 and 2018 in east Hungary lowland on acidic sandy soil, own rooted planting material. Our results showed that besides high yield and adequate cane production desired sugar content at convenient pH is to be awaited with moderate deviation between vintages. Presented concept demonstrate technological flexibility of cultivars by their average deviation from regression equation between increasing sugar and pH typical for the vintage composed of data of cultivars of the gene bank. Average positive deviation means higher sugar content at specific pH, thus higher sugar content at desired, conveniently low pH (3.0-3.2 pH).

• Microsatellite based identification of grapevine cultivars traditional in Hungary and in the Carpathian Basin

  71-73.

  Gy. D. Bisztray

  T. Deák

  C. Eisenheld

  A. Pedryc

  I. Balogh

  F. Regner

  Views:

  149

  Grapevine cultivars and clones traditional in Hungary and in the Carpathian Basin are maintained in the genetic collection of the Corvinus University of Budapest. Mostly ancient varieties and clones were genotyped using microsatellite loci. The investigated 6 loci were sufficient to distinguish all cultivars. Some clones could also be separated based mostly on the variable VVS2 loci. For 17 out of the 31 investigated cultivars this is the first report on characterization of the polymorphism of the allele lengths by microsatellite markers on loci: VVS2, VVMD7, VVMD27, vrZAG62.

• Two new registered table grape varieties

  51-53.

  E. Hajdu

  É. Ésik

  Views:

  145

  Two table grape varieties — Kósa and Narancsízű — were registered of the hybrids bred in the Research Institute for Viticulture and Enology in I lungary. Both varieties ripe early and are much in demand for the attractive clusters and flavours. As they are hybrids of Vitis vinifera they require similar growing conditions. In the same time with their qualification the two varieties are included in the variety list and allowed to be propagated.

• Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods

  79-80.

  R. Bacsó

  G. Facsar

  F. Gyulai

  Gy. D. Bisztray

  I. Velich

  Views:

  162

  About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endosperm was analysed in these subfossils, which turned to be successful mainly in the case of grapes (Vitis vinifera) and cornels (Cornus mas). Seeds of these two species contained the most endosperm and remains of the embryo. DNA was extracted with the help of DNEasy Plant Mini Kit and analysed by RAPD-PCR method. The amplification of DNA extracted from cornel seeds resulted in detecting a 1500 by fragment, which makes the comparison of these samples with present-day cornels possible.