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Deproteinized plant juice as part of circular economy: A short review and brief experimental data
23-26Views:1504As the population of the Earth is constantly growing it generates an unmet demand for protein, which is an urgent problem. The protein extraction process is a potential solution, which offers high-quality plant protein suitable for animal and human nutrition at a favorable price. The process used within our project produces green juice from the green alfalfa biomass through pressing. After the coagulation of protein from this green juice, the by-product is called DPJ (Deproteinized Plant Juices) or brown juice. Our preliminary results match the international literature, namely that brown juice take up as much as 50% of the fresh biomass in weight. To utilize this by-product is a crucial part of the process to make it environmental-friendly and financially viable as well. The examined brown juice samples came from a small-scale experiment of alfalfa varieties carried out in the experimental farm at the University of Debrecen. According to our preliminary results, brown juice has high macro- and micronutrient values, furthermore, it has a potentially high amount of antioxidant compounds. The study highlights that brown juice is suitable as an ingredient in microbiological media, in plant nutrition as a supplementary solution, for feedstock and for preparing human food supplements or functional foods. The potential utilization of all biorefinery products makes it a very appropriate technology for today’s challenges.
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Sewage sludge compost as an alternative source of phosphorus to rye in acidic sandy soil
11-18Views:63Today, the use of chemical fertilisers is significantly determined by their production and purchase costs, which are high. In contrast, phosphorus (P) is present in sewage sludge in a form that is easy for plants to absorb. Good quality sewage sludge compost (SSC) could contain a high quantity of P, together with other macro- and microelements and organic matter. The effect of regular SSC application on soil characteristics as well as plant parameters has been studied since 2003 in Nyíregyháza in a small plot experiment. Focusing on the P in the soil-plant system, our hypothesis was that SSC covers plants’ P demand through enhancing soil P content and its plant availability in the acidic sandy soil. The effect of the SSC was examined at the doses of 0, 9, 18, and 27 t ha-1 on rye as a test crop. Some soil chemical parameters (pH, soil organic matter - SOM, ammonium lactate (AL) extractable P2O5), and the relationship between plant development (green weight, shoot length), physiological parameters (SPAD index), plant shoot P content, and soil available P content were studied. The obtained data indicated that the SOM content, pH, and available P content of the treated plots increased as a result of the long-term applied SSC compared to the control. Measurement of the relative chlorophyll content showed a strong correlation with the available P content of the soil, but surprisingly less correlation with shoot P content was found. The results of plant biomass and soil P content proved that SSC could be used as a low-cost and good source of P for plants.
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Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil
111-115Views:200We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.
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Evaluation of the correlation between SPAD readings and absolute chlorophyll content of maize under different nitrogen supply conditions
121-126Views:362Currently, one of the most important objectives of agriculture is to maintain the principles of the sustainability. The use of precision technologies in agriculture belongs to this topic. The use of precision technologies is increasingly widespread in the cultivation of various agricultural crops, including maize. Sensing is an important part of these techniques. In our experiment we compared two methods: measuring relative chlorophyll content and the method of determine the extractable chlorophylls. The experimental plant was maize (Zea mays L.) and the measurements were performed at an early development stage (V8) of three genotypes. Three levels of nitrogen (0; 80; 160 kg ha-1) were applied during the experiment. The relative chlorophyll content was measured by SPAD-502 (Minolta, Japan) and a handheld GreenSeeker (Trimble, USA) device. The extractable total chlorophyll content decreased in parallel with the increased nitrogen level. The obtained SPAD values were diversified furthermore the NDVI values have not been changed for the effect of different nitrogen fertilization. In the early stages of development of maize, these parameters need to be complemented with other measurements to provide reliable information about the crops nitrogen status.
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Intensity of free radical processes in the leaves of arboreal plants under act of industrial dust borne extracts
83-87Views:65The influence of industrial pollutants on the intensity of lipid peroxidation in the assimilatory organs of arboreal plant was investigated. The differential changes of the probed indexes are set depending on the species. Information is got can testify to participation of lipid peroxidation products in forming of reactions-answers of arboreal plants on influence of industrial dust borne extract with content of heavy metals. Determination of level and rates of accumulation of Zn, Ni, Pb and Cd, in the leaves of arboreal plants in the conditions of different
contamination level allowed to take species to two groups. To the first (phytoextraction potential exceeds a base-line level in 10 times) belong Populus bolleana Lauche, P. italica (Du Roi) Moench, Picea pungens Engelm and Sorbus aucuparia L. To the second (exceeds a base-line level from 5 to 10 times) belong Acer negundo L., Aesculus hippocastanum L., Betula pendula Roth and Tilia cordata Mill. The most substantial increase of peroxidation secondary product content (more than in 2.5 times) is peculiar for B. pendula, A. hippocastanum and P. pungens Engelm., that well conforms to the rates of heavy metals translocation, it has however species-specific character. -
Violation prooxidative-antioxidant stability at maize shoots at different level of accumulation of cadmium and nickel
89-94Views:68Joint influence of cadmium and nickel was investigated on the feature of their accumulation by the vegetative organs of 10-days' old maize shoots. It was established that most intensively noted metals are taken in by the roots of shoots in the first 7 hours stressing influencing, while in leaves they appear only after a 7-hour long exposition. It was stated that the absorption process of the noted metals by a root system is carried by two-phase character. The indexes of inner-tissue contamination are calculated. Activating by the cadmium and nickel ions of lipid peroxidation as marker of the stressing influencing, and also was shown the proper increase of intensity of functioning of ascorbate peroxidase as the antioxidant enzyme protection of cell.
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Identification and specific variety of actinomyces of streptomycetes genus in some chernozems of Ukraine
67-74Views:64Is definite the quantitative and quality composition of chernozem usual and southern streptomycetes cenosis. It is rotined that humus horizons of chernozem usual more biogenic, than chernozem southern. Analysis of specific structure of streptomycetes association and calculation of some biodiversity indexes by Margalef, Berger-Parker and Serensen it was allowed to set the specific features of forming of these microorganisms cenosis in investigated soils.
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Comparison of the physiological responses of an oil-pumpkin cultivar and hybrid under different nitrogen supply conditions
65-69Views:190Cucurbita pepo var. styriaca is known as a medicinal crop among other cucurbits. The benefits of cucurbits fruits are very important in terms of human health, purification of blood, removal of constipation, digestion and supplying energy. The mutant styrian oil-pumpkin (Cucurbita pepo L. convar. Pepo var. styriaca Greb.) exposes a complete lack of lignification of the seed testa. For this reason, this kind of naked pumpkin seed is more consumable as snack than other seeds. The vegetative growth of plants is crucial for fruit production. Nitrogen is the most imperative element for the proper vegetative growth and development of plants which significantly increases and enhances yield and its quality by playing a vital role in the biochemical and physiological functions of plants.
Field studies were conducted to study the effect of nitrogen nutrition on certain physiological parameters and their differences between the styrian oil pumpkin hybrid and cultivar (Gleisdorfi Classic cultivar and GL Rustical hybrid). The small block experiment was carried out based on a factorial experiment with a completely randomised block design and four replications in the Research Institute of Nyíregyháza during the summer period of 2017 and 2018. Three different concentrations of nitrogen fertiliser was applied at sowing. Photosynthetic pigments (chlorophyll-a, -b and carotenoids) were analysed as one of the basic factor of efficient photosynthesis. The yields of two genotypes were also measured in all treatments as seed-weight. The contents of photosynthetic pigments were higher in the oil-pumpkin hybrid, mainly the carotenoids in 2018. The vegetation period of 2018 was drier than in 2017, the hybrid oil-pupmkin was more efficient in stress tolerance than the cultivar with higher carotenoids pool. The obtained yield was more pronounced in the oil-pumpkin hybrid than the cultivar in all examined years. The hybrid GL Rustical was more sensitive to nitrogen treatment than the cultivar Gleisdorfi Classic.
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Growth regulators influence on stability of shoots and ascorbic acid content at cadmium and nickel joint action
9-12Views:118It is shown that for a maize the most effective protector of cadmium and nickel influence was zeastimulin, for a pea – agrostimulin.A protective function of emistim C for both cultures was insignificant. It is set that zeastimulin is influential in the increased metals absorption of a root system, however substantially (on 15% for a nickel and twice for a cadmium) reduces their translocation to above-ground part of plants. Previous treatment of pea seed by agristimulin also intensified the accumulation of nickel roots on 60% and did not influence on cadmium absorption and toxicants translocation to tissues of assimilatory organs. The protector role of growth regulators to cadmium and nickel joint influence shows up in the increase of ascorbic acid maintenance in the roots cells of both species to 34%, where as in leaves – at a maize resulted in the lowering of vitamin С amount (on 28%), but at a pea – increase on 20%.
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Food safety of plant origin food
161-164Views:173Expectation regarding changes related to food production, transportation, distribution and marketing have changed considerably in several regions of the world over the past decades. It is especially true for highly industrialized countries where not only the food security is important but the food safety is essential too. The concentration of production – processing – distribution involves the danger on the one hand products of unsuitable quality from point of view of nutritional physiology and the other hand ones that may contain substances harmful to human health will become widespread among consumers. We investigated the heavy metal, pesticide remains and mycotoxin content of different plant products.
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The effects of drought stress on soybean (Glycine max (L.) Merr.) growth, physiology and quality – Review
19-24Views:200Abiotic stresses are one of the most limiting factors inhibit plant's growth, leading to a serious production loss. Drought stress is one of the most destructive abiotic stresses and is still increasing year after year resulting in serious yield losses in many regions of the world,
consequently, affecting world’s food security for the increasing world population. Soybean is an important grain legume. It is one of the five major crops in the world, an essential source of oil, protein, macronutrients and minerals, and it is known as the main source of plant oil and protein. Harvested area of soybean is increasing globally year after year. However, soybean is the highest drought stress sensitive crop, the water deficit influences the physiology, production and seed composition of this crop. We introduce a review for literatures concerning the changes of the above traits of soybean exposed to drought stress, with past explanations for these changes. -
Examination of Zn deficiency on some physiological parameters in case of maize and cucumber seedlings
5-9Views:85Zinc (Zn) is an essential micronutrient needed not only for people, but also crops. Almost half of the world’s cereal crops are deficient in
Zn, leading to poor crop yields. In fact, one-third (33%) of the world's population is at risk of Zn deficiency in rates, ranging from 4% to
73% depending on the given country. Zn deficiency in agricultural soils is also a major global problem affecting both crop yield and quality.
The Zn contents of soils in Hungary are medium or rather small. Generally, the rate of Zn deficiency is higher on sand, sandy loam or soil
types of large organic matter contents. High pH and calcium carbonate contents are the main reasons for the low availability of Zn for
plants (Karimian and Moafpouryan, 1999). It has been reported that the high-concentration application of phosphate fertilisers reduces Zn
availability (Khosgoftarmanesh et al., 2006). Areas with Zn deficiency are particularly extensive in Békés, Fejér and Tolna County in
Hungary, yet these areas feature topsoils of high organic matter contents. Usually, Zn is absorbed strongly in the upper part the soil, and it
has been observed that the uptakeable Zn contents of soil are lower than 1.4 mg kg-1.
Maize is one of the most important crops in Hungary, grown in the largest areas, and belongs to the most sensitive cultures to Zn
deficiency. Zn deficiency can causes serious damage in yield (as large as 80 %), especially in case of maize. On the other hand, Zn
deficiency can also cause serious reduction in the yields of dicots. One of the most important vegetables of canning industry is cucumber,
which is grown all over the world.
In this study, the effects of Zn deficiency have investigated on the growth of shoots and roots, relative and absolute chlorophyll contents,
fresh and dry matter accumulation, total root and shoot lengths, the leaf number and leaf area of test plants in laboratory. Experimental
plants used have been maize (Zea mays L. cv. Reseda sc.) and cucumber (Cucumis sativus L. cv. Delicatess). A monocot and dicot plant have
chosen a to investigate the effects of Zn deficiency, because they have different nutrient uptake mechanism.
It has been observed that the unfavourable effects of Zn deficiency have caused damage in some physiological parameters, and
significantly reduced the growth, chlorophyll contents of monocots and dicots alike. -
The phosphate state and biochemical mobilization of phosphorus compounds in arboreal plants’ soils
95-98Views:79Some indices of the phosphoric fractions of primery degraded soils, which are formed separate areas of technogenic landscapes, on a spoil-bank of iron-ore mine in the near of Kryvyi Rig, under act of lignosa, which are used for biological recultivation of degraded soils are investigated. Maintenance of mineral phosphates and features of organic phosphorus accumulation are set in soil under arboreal planting. Nutrient supply of plants is enhanced by mobile phosphates and their dynamics during vegetation period. Activity of alkaline and acid phosphatase enzymes are concerned also. On the basis of the soil enzymes activity information it is stated, that under the 35-years-old plantage of Robinia pseudoacacia L. the biochemical mineralization of organic phosphorus compounds passes considerably more actively than under Pinus pallasiana D.Don.
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The Effect of Smut Gall Tumour Infection on Iron and Zinc Uptake and Distribution in Maize Seedlings
27-32Views:90The amounts of Fe, and Zn were measured in maize seedlings infected by smut gall tumour (Ustilago maydis Dc. Cda.) and in healthy seedlings five days after infection. The amount of elements was also measured under different stress intensities. Due to the infection, as a biotic stress, the amount and distribution of examined elements have been changed. On the bases of the differences in the Fe distribution between the symptoms less and tumorial leaf parts, we have come to the conclusion that the infection also effects the mobilisation of Fe and Zn inside the plants. The Fe uptake was much higher in the infected plants and the tumour development also had an effect on the uptake and distribution of the examined elements. The experiments of infecting maize seedlings by monosporidial strain of crown gall tumour showed no tumour development. We found that the monosporidial strain also acts as a biotic stress and has an effect of iron and zinc distribution. We observed a slight difference in the iron and zinc contents in the roots of corn seedlings infected by different monosporidial sporidium concentrations, while the iron and zinc contents in the shoots were increased by the intensity of the infection. The roots do not form tumours. There is no difference between the roots of the infected and healthy corn seedlings. Since the Fe and Zn contents of the shoots of infected plants depend on the intensity of the infection, we have come to the conclusion that there must exist a „special” communication system regulating the transportation of the examined elements.
In the experiments with infected maize seedlings, it became necessary to get the iron chlorosis before the disease reaches the lethal phase. Although most of the iron reserves are located in the embryo, to accelerate the chlorosis, the endosperm was removed, and it was observed, that the iron chlorosis appears later in maize seedlings when the endosperm is removed. The relative chlorophyll content of the first and second leaves was measured in iron efficient and iron deficient maize seedlings at different times.
The higher IAA content of tumorial plant tissues is already known. The treatment with IAA decreases the iron concentration in the shoots and in the roots of +Fe precultured plants and increases at -Fe precultured ones. The TIBA retards the shoot-to-root transport of IAA. When the seedlings were treated simultaneously with IAA and TIBA, higher iron concentrations were observed in the shoots and in the roots of corn seedlings.
We found extremely high iron concentrations in the roots of infected seedlings and, in line with this, serious damage to the roots was observed that this can be caused by the high iron content generated free radicals. The results demonstrate that IAA has a role in the shoot to root communication. -
Isolation of promoters of tissue and ripening-specific strawberry genes by TAIL-PCR and bioinformatic characterization of the sequences
91-99Views:55Isolation of ripening- and tissue-specific promoters has become a very important subject of the genetic regulation and plant physiology research in recent years. It could be possible to reveal the regulation of gene expression, and it may be a very useful approach in the biotechnology. In our work, we have isolated promoter regions of genes exhibiting ripening- and tissuespecific expression in our previous experiments, and the data were
characterized by bioinformatic methods. In the sequence of the ripening-specific Spatula and ACC-oxidase promoters (ACCoxidase is one of the key-enzymes of ethylene biosynthesis, directly related to the process of ripening); we could identify auxin- and ethylene-related cis-regulatory elements. This suggests that there is an interaction between ripening and ethylene-synthesis, in case of non-climacteric strawberry, too. We investigated the promoter regions of three green receptacle-specific genes (putative nitrilaselike protein, Ring transcription factor and an aquaporin protein)
and we could identify several regulatory elements, which refer to hormonal regulation. Additionally, we could find several cisacting elements which associated with stress-responsiveness and endospermium-specific expression. -
Preface
5Views:32In the frame of a common “Hungarian-Ukrainian Intergovernmental S&T Cooperation Programme” which title is “Change of soils ecological characteristics of Ukraine and Hungary in the conditions of anthropogenic transformed ecosystems and optimization of biological processes of plants primary feeds elements mobilization” a Workshop was held in Debrecen. The member institutes of project participated with different presentation in this program.
The title of Workshop was: “Anthropogenic effect on the properties of Middle and Eastern European chernozem soils and on the sustainable agricultural production”.
The aim of the Workshop was to give relevant information about the present situation of the Middle and Eastern European Chernozem soils, especially emphasize the effect of different loading on the quality (properties) of chernozem soils. With the Workshop we would like to create a tradition for discussion about the anthropogenic effect on the soil properties and through it on the productivity of different soils. It was a forum for discussion of research results related to problems and possibilities for prevention of soil quality. With this possibility we would like to contribute to the sustainable agricultural production.
The papers were read for the publisher and we would like to show them in a separate supplement of Journal of Agricultural Sciences, Acta Agraria Debreceniensis as one of the results of the project.
The papers comply with the requirements of the scientific issue except those two which show the university and the department of the Ukrainian partner taking part in this project.
The participant Institutes of the project:
- Dnepropetrovsk National University, Faculty of Biology and Ecology;
- Kryvyi Rig Botanical Garden NAS of Ukraine, Plant Physiology & Soil Biology Department;
- Department of Agrochemistry and Soil Sciences of Centre for Agricultural and Applied Economics;
- Research Institute of Karcag, Centre for Agricultural and Applied Economics. The collaboration with Ukrainian partners was successful and we have confidence in the further cooperation in scientific research. -
Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:140Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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Species-specificity of the glutathione reduced form accumulation in the arboreal plants, testing influence of industrial emissions
105-110Views:72Conducted are researches of dynamics of glutathione reduced form maintenance in the leaves of arboreal plants which grow in the zones of different level of contamination by industrial emissions of“Red Lead Factory” LTD and ArselorMittal Kryvyi Rig. For comparison, similar investigations were conducted in the intact Botanic Garden in s.c.t. Sofievka. Findings testify to absence of negative influence of industrial emissions and, thus, the arboretum of botanical garden also can be examined as conditional control. It was stated that it is set a few strategies of active oxygen forms and their metabolites deactivation, appearing in cells under action of environment negative factors. To our opinion, fluctuation of glutathione reduced form maintenance can examine as an index of plants stability, on which investigational species can be distributed on steady and moderato steady.
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Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds
99-104Views:96Contamination of chernozem by fluorine compounds variously affects those enzymes (urease, asparaginase, glutaminase, arginase, amidase), which takes part in the metabolism of nitrogen-bearing organic compounds. In broken soils the inhibited desaminisations is stronger, than enzymatic hydrolysis of asparagine and arginine. The features of seasonal dynamics of change activity of urease and correlation dependence of its activity from some physical and chemical soils properties are described. These tendencies well comport with the results of model experiments. At minimum HF influence there is inhibition of processes of monohydrocarboxylic acids desaminisation, hydrolytic breaking up of arginine and glutamine. By a side with this there is activating of urea and asparagine breaking up processes on the initial stages of toxicant influence. The study of kinetics of process of urea enzymatic hydrolysis in chernozem at the different level of HF influence showed changes of initial and maximal velocity of enzymatic reaction, and also Michaelis-Menten constant.
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Precision crop production and artificial intelligence – the future of sustainable agriculture
47-58Views:405According to Kay et al. (2004, in Shockley et al., 2017), there are seven steps to the decision-making process: 1) Identify the problem or opportunity, 2) Identify the alternative solution, 3) Collect all data and information, 4) Analyse the alternatives and make a decision, 5) Implement the decision, 6) Monitor the results of the decision, 7) Accept responsibility for the decision. The basic question is what kind of tasks we can perform in the decision-making process and what to leave for Artificial Intelligence (AI).