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Identification and specific variety of actinomyces of streptomycetes genus in some chernozems of Ukraine
67-74Views:61Is definite the quantitative and quality composition of chernozem usual and southern streptomycetes cenosis. It is rotined that humus horizons of chernozem usual more biogenic, than chernozem southern. Analysis of specific structure of streptomycetes association and calculation of some biodiversity indexes by Margalef, Berger-Parker and Serensen it was allowed to set the specific features of forming of these microorganisms cenosis in investigated soils.
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Intensity of free radical processes in the leaves of arboreal plants under act of industrial dust borne extracts
83-87Views:61The influence of industrial pollutants on the intensity of lipid peroxidation in the assimilatory organs of arboreal plant was investigated. The differential changes of the probed indexes are set depending on the species. Information is got can testify to participation of lipid peroxidation products in forming of reactions-answers of arboreal plants on influence of industrial dust borne extract with content of heavy metals. Determination of level and rates of accumulation of Zn, Ni, Pb and Cd, in the leaves of arboreal plants in the conditions of different
contamination level allowed to take species to two groups. To the first (phytoextraction potential exceeds a base-line level in 10 times) belong Populus bolleana Lauche, P. italica (Du Roi) Moench, Picea pungens Engelm and Sorbus aucuparia L. To the second (exceeds a base-line level from 5 to 10 times) belong Acer negundo L., Aesculus hippocastanum L., Betula pendula Roth and Tilia cordata Mill. The most substantial increase of peroxidation secondary product content (more than in 2.5 times) is peculiar for B. pendula, A. hippocastanum and P. pungens Engelm., that well conforms to the rates of heavy metals translocation, it has however species-specific character. -
Violation prooxidative-antioxidant stability at maize shoots at different level of accumulation of cadmium and nickel
89-94Views:66Joint influence of cadmium and nickel was investigated on the feature of their accumulation by the vegetative organs of 10-days' old maize shoots. It was established that most intensively noted metals are taken in by the roots of shoots in the first 7 hours stressing influencing, while in leaves they appear only after a 7-hour long exposition. It was stated that the absorption process of the noted metals by a root system is carried by two-phase character. The indexes of inner-tissue contamination are calculated. Activating by the cadmium and nickel ions of lipid peroxidation as marker of the stressing influencing, and also was shown the proper increase of intensity of functioning of ascorbate peroxidase as the antioxidant enzyme protection of cell.
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Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds
99-104Views:86Contamination of chernozem by fluorine compounds variously affects those enzymes (urease, asparaginase, glutaminase, arginase, amidase), which takes part in the metabolism of nitrogen-bearing organic compounds. In broken soils the inhibited desaminisations is stronger, than enzymatic hydrolysis of asparagine and arginine. The features of seasonal dynamics of change activity of urease and correlation dependence of its activity from some physical and chemical soils properties are described. These tendencies well comport with the results of model experiments. At minimum HF influence there is inhibition of processes of monohydrocarboxylic acids desaminisation, hydrolytic breaking up of arginine and glutamine. By a side with this there is activating of urea and asparagine breaking up processes on the initial stages of toxicant influence. The study of kinetics of process of urea enzymatic hydrolysis in chernozem at the different level of HF influence showed changes of initial and maximal velocity of enzymatic reaction, and also Michaelis-Menten constant.