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Comparison of Scab Warning Systems in Integrated Apple Production
53-57Views:54In the present study, the suggestions for infection risk and sprays of the most frequently used PC-based scab warning systems (RIMpro and WELTE) and the conventionally used Mills table as a reference were compared in integrated apple production. The efficacy of the spraying programs based on the three different warning systems was evaluated by assessment of apple scab incidences during primary infection periods.
13 and 11 Mills infection periods were detected in 2000 and 2001, respectively, during primary infection periods (from 15 March until 5 June). Taking into consideration the heaviness of Mills infection periods, 11 and 9 sprays were applied in 2000 and 2001, respectively. The two PC-based scab warning systems detected 1-4 less infection periods and suggested 1-3 less sprayings compared to the numbers of infection periods and sprays according to the Mills table. Our results proved that the 9-11 sprays according to the Mills table could be reduced to 7-9 sprays by RIMpro scab warning systems which did not reduce the efficacy and did not increase the symptoms of leaf and fruit scab significantly. The WELTE scab warning systems detected generally one or two more infection periods and suggested one additional spray compared to RIMpro warnings and sprays. -
Changes in the Diaporthe helianthi Infection of Sunflower Hybrids Between 1998 and 2002
214-233Views:65Plant protection, and especially the efficiency of protection against mycosis, is a very important production technological element concerning sunflower. The efficiency of production can be increased on the basis of a thorough pathological survey and its results carried out in a wide variety of hybrids, as the features of hybrids, the reactions of genetic bases to pathogens can be found out under domestic conditions.
The tests were carried out at variety-test lots of OMMI for hybrids used for food or oil and other sunflower varieties admitted by the state at the Experimental Site DTTI Látókép, Centre of Agricultural Sciences, University of Debrecen between 1998 and 2002. The number of the tested hybrids was 49 in 1998, 45 in 1999, 49 in 2000, 55 in 2001, and 44 in 2002.
Due to the infection source of high amounts remained from 1997 and the favourable weather conditions for the pathogens, an infection of high degree was experienced in the tested stands. The lowest infection index in the hybrids with a very short vegetative period was experienced with Beni hybrids. Comparing to the average of the hybridgroup with short vegetative period, a low infection index characterised the Baleno, Trident, IBH-166, Hysun 321, Resia, Alexandra, Cergold and Pixel hybrids. In 1999, among hybrids with medium vegetative period, Zoltán, Zsuzsa and Util hybrids could be highlighted because of their low infection index. In 1998, among confentionary hybrids, Marica-2 hybrid had the most favourable index values (2,55).
During our trials, the experienced infection dynamics were compared in the event of hybrids with higher and lower susceptibility under different sowing technological elements. The results call for the fact that when a hybrid with higher susceptibility is produced, production technological elements, such as the time of sowing, influence considerably the damage caused by the pathogen, therefore it must not be ignored when its determination takes place. In the event of hybrids with lower susceptibility the change of the tested production technological elements within the biological optimum does not lead to the increased risk of the damage caused by Diaporthe helianthi. -
Study of plant production modells with different intensity in winter wheat production
51-60Views:91The effects of crop rotation, nutrient supply and plant protection technologies were examined on the yield of Mv PÁLMA winter wheat variety and on the most important diseases of ear and leaf. Our experiments were carried out on chernozem soil in the Hajdúság in 2006 and 2007, and three plant protection technologies (extensive, average, intensive) and three irrigation variations (without irrigation, irrigated with 50 mm, irrigated with
100 mm) were applied in different crop rotation systems.
In the triculture crop rotation a higher rate of infection was observed than in the biculture crop rotation, because the vegetative growth was more expressed after pea and these microclimatic factors were favourable for the development of pathogens.
In the triculture crop rotation (pea – wheat – maize) the powdery mildew, DTR and leaf rust of wheat were present in both examined years (powdery mildew 5-15%, DTR 14-42%, leaf rust 8-37% in cropyear 2005/2006, powdery mildew 12-32%, DTR 9-29%, leaf rust 8-26% in cropyear 2006/2007). Fusaria could be observed in 2006 (depending on the plant protection technologies and nutrient supply in the biculture 7-27% and in the
triculture 5-19%). With higher amounts of fertilizers the rate of infection increased and reached its maximum at the highest dose of nutrient supply (N200 +PK).
We observed the highest rate of infection by ear and leaf diseases in the case of the extensive technology, while this rate could be considerably reduced by the application of the intensive technology.
Both in 2006 and 2007, yields were the highest at the N100-200+PK levels in the triculture after pea (6028-7939 kg ha-1 in cropyear 2006, 6578-8690 kg ha-1 in cropyear 2007 depending on plant protection technologies), and at the N150-200+PK levels in the biculture after maize (6096-7653 kg ha-1 in cropyear 2006, 4974-8123 kg ha-1 in cropyear 2007 depending on the plant protection technologies). The highest yield maximums were
reached when pea was the forecrop. The yields on the experimental plots of the intensive plant protection technology was 224-2198 kg ha-1 higher (depending on the forecrop) compared to the plots where the extensive technology was used.
The highest yield without irrigation was at the N150+PK both in biculture and triculture crop rotation. Among the irrigated variations Ö2 and Ö3 at N200+PK fertilisation resulted in the highest yield in the biculture crop rotation, while the N100+PK level in triculture system. In the biculture crop rotation the extra yield was 14-51% higher (575-1225 kg ha-1 depending on plant protection technology) when 50 mm water was irrigated, and
15-54% higher (778-2480 kg ha-1) if 100 mm irrigation was applied comparing to the non-irrigated versions. The yield was 7-17% higher (560-1086 kg ha-1) in the Ö2 irrigation variation, and 8-23% (691-1446 kg ha-1) higher in the Ö3 irrigation variation compared to Ö1 irrigation variation (non-irrigated).
A correlation analysis was made to reveal the connection between the yield, the amount of fertilizers, the rate of infection, the plant protection technologies and the forecrops. Strong positive correlation (0.846) was found between year and fusaria infection. Strong positive correlation was observed between fertilization and powdery mildew infection (0.525), fertilization and DTR (0.528), fertilization and yield quantity (0.683). Lower
correlation was found between fertilization and leaf rust infection (0.409), and forecrop and yield (0.472), recpectively. Negative correlation was calcutated between plant protection technologies and DTR (-0.611), and plant protection technologies and leaf rust (-0.649). -
The Effect of Forecrop and Plant Protection on the Pathology Parameters and Yields of Winter Wheat
84-89Views:88We carried out our experiment in the cropyears of 2000/2001, 2001/2002 and 2002/2003, on calcareous chernozem soil, at the experimental site of the Debrecen University Farm and Regional Research Institute, at Látókép. We examined the disease resistance and the yield quantity of Mv Magvas variety by adopting different forecrops and plant protection technologies, at 30+30 N level and at normal cereal row spacing. We applied two forecrops (wheat and pea) and two plant protection technologies (extensive and intensive). We measured the rate of infection by population survey in the first ten days of June.
In the course of our examinations, we found, that the rate of powdery mildew infection was higher in the thicker population sown after pea forecrop in all three years, as powdery mildew is not a typical cereal disease.
The infection rate of leaf mildew and DTR (Dreschlera tritici-repentis) was higher after wheat forecrop in all examined years, because these are typical wheat diseases and infection centres in the soil promote the spreading of these diseases. However, it was possible to parry the adverse effect of forecrops by intensive plant protection.
Due to the chernozem soil, wich has good water management features, and due to the good preparation of the seedbed, the effect of forecrops on yield quantity did not appear in the examined years. The quantity of the yield was only slightly larger after pea forecrop in the cropyears of 2000/2001 and 2002/2003 than after wheat. Nonetheless, the data of technical literatures state that the yield quantity can be larger, even by 15-20%, after pea forecrop.
In the course of intensive plant protection technology, we applied systemic pesticides, while in the course of environmentally sound technology, we used contact pesticides of sulphur content. In those populations that were treated with environmentally sound plant protection technology, infection rate was higher in all three years.
Yield quantities were somewhat lower in the course of applying extensive, environmentally sound technology, because diseases appeared in these populations to the higher degree. Powdery mildew does not, but leaf mildew and Dreschlera tritici-repentis have a significant yield decreasing effect. With appropriate, well-selected fungicides, we were able to keep every leaf diseases well in hand, and the rate of infection was almost independent of the influence of the breeding year. -
The Effect of Plant Density on the Yield of Sunflower Hybrid’s in 2001-2002
184-189Views:89Nowadays, for increasing efficiency of sunflower production treating hybrid-specific technologies was required. Increasing of hybrid choice gave reasons for trials in respects of critical factors, as well as in case of genotype-enviroment interactions. The effect of changing plant density show up as determinant factor which affects on yield as well as on plant hygienic conditions. Trials were established on calcareous chernozem soil (Hajdú-Bihar county), in 2001-2002. The field trials were randomized, in four repetition on small parcels. The plant density trials were established in 35.000-75.000 plant/hectar interval using a scale of 10.000 plant/hectar. 10 hybrids were used in both year.
In 2001, 55.000 plant/hectar density was the optimal, in case of most of the hybrids. The yield of tested hybrids did not show significant difference. Yield decreasing effect of using less than optimal density was more significant than in case of using optimal plant density. In 2002, the optimal density was in 45.000-65.000 plant/hectar interval, there was no possibility to find narrower optimum.
Presence of Diaporthe helianthi and the damage caused by the pathogen was significant in 1997-1999, whereas in 2001-2002 the large-scale appearance of the pathogen did not occur. In 2001, the affect of high plant density on disease caused by Diaporthe helianthi showed just tendency-like appearance, in 2002, the infection showed up just in the end of the vegetation period, without significant damage. In spite of the low infection level, the difference between the infection of the diverse plant density treatment was significant. Increasing plant density made increase the frequency of the Diaporthe helianthi infection. The rate of the flower diseases was around 3-16% in 2001, the highest infection level emerged in 65.000-75.000 plant/hectar density, whereas in 2002, the rate of infection level was less than 10%. The increasing plant density helped the development of flower diseases. -
Effect of two different cropyear and the agrotechnological factors on the agronomic characteristic of the winter weat in a lon-term experiment
143-149Views:108We studied the effects of crop rotation, fertilization and crop protection technologies on occurence of the major ear- and leaf-diseases (powdery mildew, helminthosporiosis, leaf rust, Fusarium wilt) and the degree of lodging in the winter wheat variety Mv Pálma in two very different years (2006/2007=dry; 2009/2010=rainy). The experiments were carried out at the Látókép Experimental Farm of the University of Debrecen CAAES in triculture (pea-wheat-maize) and biculture (wheat-maize) at five fertilization levels by applying three different crop protection technologies (extensive, average, intensive).
In the cropyear of 2006/2007, the disease severity of leaf diseases was higher than the average in both crop rotations in spite of the fact that the weather during the whole vegetation period was dry. Infection by powdery mildew, helminthosporiosis and leaf rust increased with increasing fertilization, the highest infection was measured at the highest fertilization level (N200+PK) in the extensive crop protection technology. According to the results, no infection of ears by Fusarium and no lodging occurred in either bi- or triculture due to the dry year. The extremely rainy weather in 2009/2010 was favourable not only for the vegetative development of the stand, but also increased the occurance of leaf-, stalk- and ear-diseases and a high degree of lodging was observed. The highest infection by powdery mildew was observed in the plots with the highest fertilizer dosage under an extensive crop protection technology. A much higher helminthosporiosis infection was measured than in the cropyear of 2006/2007. The wet weather and higher than average temperature promoted the occurence and spreading of leaf rust. Under an extensive crop protection technology, a leaf rust infection of 24% and 31% was detected after maize and pea as a forecrop, respectively, in the N200+PK treatment. As opposed to 2006/2007, the disease severity of Fusarium was 3–8% and 2–7% in the control after maize and pea as a forecrop, respectively. This value, similarly to that of other pathogens, increased with increasing fertilization levels. Due to the large vegetative mass, a significant lodging was observed in the wheat stands in both bi- and triculture (17–100%, 12–100%). -
The Effect of Smut Gall Tumour Infection on Iron and Zinc Uptake and Distribution in Maize Seedlings
27-32Views:87The amounts of Fe, and Zn were measured in maize seedlings infected by smut gall tumour (Ustilago maydis Dc. Cda.) and in healthy seedlings five days after infection. The amount of elements was also measured under different stress intensities. Due to the infection, as a biotic stress, the amount and distribution of examined elements have been changed. On the bases of the differences in the Fe distribution between the symptoms less and tumorial leaf parts, we have come to the conclusion that the infection also effects the mobilisation of Fe and Zn inside the plants. The Fe uptake was much higher in the infected plants and the tumour development also had an effect on the uptake and distribution of the examined elements. The experiments of infecting maize seedlings by monosporidial strain of crown gall tumour showed no tumour development. We found that the monosporidial strain also acts as a biotic stress and has an effect of iron and zinc distribution. We observed a slight difference in the iron and zinc contents in the roots of corn seedlings infected by different monosporidial sporidium concentrations, while the iron and zinc contents in the shoots were increased by the intensity of the infection. The roots do not form tumours. There is no difference between the roots of the infected and healthy corn seedlings. Since the Fe and Zn contents of the shoots of infected plants depend on the intensity of the infection, we have come to the conclusion that there must exist a „special” communication system regulating the transportation of the examined elements.
In the experiments with infected maize seedlings, it became necessary to get the iron chlorosis before the disease reaches the lethal phase. Although most of the iron reserves are located in the embryo, to accelerate the chlorosis, the endosperm was removed, and it was observed, that the iron chlorosis appears later in maize seedlings when the endosperm is removed. The relative chlorophyll content of the first and second leaves was measured in iron efficient and iron deficient maize seedlings at different times.
The higher IAA content of tumorial plant tissues is already known. The treatment with IAA decreases the iron concentration in the shoots and in the roots of +Fe precultured plants and increases at -Fe precultured ones. The TIBA retards the shoot-to-root transport of IAA. When the seedlings were treated simultaneously with IAA and TIBA, higher iron concentrations were observed in the shoots and in the roots of corn seedlings.
We found extremely high iron concentrations in the roots of infected seedlings and, in line with this, serious damage to the roots was observed that this can be caused by the high iron content generated free radicals. The results demonstrate that IAA has a role in the shoot to root communication. -
Yearly change of Wheat dwarf virus infection rate during 1996-2010 in winter barley
22-28Views:95Yearly change of the infection of Wheat dwarf virus was studied in winter barley during 1996-2010. Surveys were carried out at Kompolt (Rudolf Fleischmann Research Institute, Róbert Károly College), in winter barley breeding lines showing leaf yellowing and stunting symptoms. In 1996, 250 winter barley samples were tested. During the period of 1997–2005, 100 samples were collected in each year. In 2006, 490 winter barley samples were tested. In 2007 and 2008 the number of samples collected was 500 from winter barley. In 2009 year 100, and in 2010 year 100 winter barley samples were collected for virus testing. Virus diagnosis was carried out using DASELISA for the detection of Wheat dwarf virus (WDV), Barley yellow dwarf viruses (BYDV-MAV, BYDV-PAV, BYDV-RMV, BYDV-SGV), and Cereal yellow dwarf virus (CYDV-RPV). During the ten of the last fifteen years, the occurrence of Wheat dwarf virus in infected samples exceeded those of other viruses causing leaf yellowing and dwarfing symptoms. There were years (1997, 2002, 2004, 2007, 2009 and 2010) when only the Wheat dwarf virus played the main role in development of yirus symtoms. A contrasting tendency can be observed between the degrees of infection of WDV and BYDV. With a rise of infection in the WDV, the proportion of BYDV decreased and vice-versa.
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The effect of fertilization and plant protection in sunflower (Helianthus annuus L.) production
57-63Views:220Sunflower is the most important oil crop in Europe which is grown on the biggest area of all the oil crops. The area of producing sunflowers in Hungary was changing to 524–704 thousand hectares in the past decade in comparison with approximately 100 thousand hectares in the 1970’s. In our experiment different sunflower hybrids were examined. The doses of fertilizers were the following in 2017 and 2018: 0–30–90–150 kg ha-1 N, 0-50-90-90 kg ha-1 P2O5 and 0–70–110–110 kg ha-1 K2O. Three different treatments of plant protection were used in the experiment to protect them from fungal infections. A rise in the dosage of nitrogen resulted in increasing infection. The biggest fungal infection was identified in 150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg ha-1 K2O treatment. The largest infection was measured on plots without plant protection at the highest N doses, both in leaf and disc diseases. We measured the highest 41.25% in 2017 and 53.1% in 2018 Diaporthe helianthi infection, and 24.5% in 2017 and 25.5 % in 2018 Sclerotinia sclerotiorum disc infection in these plots. kg ha-1
Average yield was changing between 2.96–4.67 t ha-1 in 2017/2018. The lowest yield was obtained in the absolute control plot (without plant protection and without nutrient) in both years, which was 2.96 t ha-1 in 2017 and 3.14 t ha-1. The yields increased due to growing nutrient supply and excellent plant protection. The highest yields were 3.67 t ha-1 in 2017 and 4.67 t ha-1 in 2018 (150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg K2O active substance). Purpose of the experiment at analysing the impact of different treatments on the profitability of sunflower production.
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Utility of Winter Wheat According to Visual and Microbiological Fusarium Infection, as Well as Toxin Examination
26-34Views:90In 1998 the Fusarium infection was studied visually and microbiologically and also F-2, T-2, HT-2, DAS and DON contamination were measured using 22 winter wheat samples. The correlation between the different parameters of 22 wheat samples were determined by regression analysis. According to our results we can state the following.
There is no significant connection between the results of visual, microbiological and toxicological examinations. This means that no certain conclusion can be drawn about the toxin contamination of samples – which is a determining factor of its utility – based on the visual symptoms and the plate dilution method.
Our results indicate however – though it is not proven statistically – that those samples in which the Fusarium infection did not exceed the limit of the standard, also had low toxin contamination, therefore they can be used as components of forage.
It is a considerable problem, however, that according to the visual qualification, such samples are excluded from the later utilisation, wherein the toxin contamination does not justify such action. Therefore, it is necessary to examine the toxin content of those samples which show high infection by visual symptoms. To reducing the number of expensive toxin examinations it would be advisable to change the currently used 0,5% limit which is indicated in the standard for a higher value of infection, for example to 2%, as recommended by Mesterházy (1998). -
The response of sunflower hybrids to different plant densities on a chernozem soil
123-128Views:147In our experiment the reaction of six sunflower hybrids of different genotypes (NK Oktava, ES Biba, ES Diagora, ES Ballistic, EGH 8925, PR 64 H 42) towards plant density has been investigated by different fungicide treatments in the crop-year of 2011 on a chernozem soil.
In the crop-year of 2011 sunflower populations were infected by a significant Diaporthe helianthi disease. The extent of this infection was significantly enhanced by the increment of plant density. However, regarding the average of the hybrids and plant densities the two times executed fungicide treatment has decreased the infection rate by 22%. The most susceptible hybrid was the ES Biba. Contrarily, the hybrid ECH8925 proved to be the most resistant hybrid towards this
disease according to our results. According to the results of the Pearson’s correlation analysis it has been revealed that stalk breakage and Diaporthe infection stand in a very close (r=0.782**) and middle close (r=0.523**) correlation resp. with plant density. The relationship between fungucude treatments and stalk breakage, just as Diaporthe infection showed to be middle and close respectively. Our results demonstrate the role of stalk and plate diseases (among them Diaporthe) in causing stalk breakage, for we have found a close positive correlation between stalk breakage and Diaporthe infection (r=0.624**) in our analysis.
From the aspect of yield amount the optimal plant density varied between 45 000 and 55 000 plants per hectare. Fungucude treatments enabled not only the use of higher plants densities, but they had a yield increasing effect as well. In the crop-year of 2011 the highest yield (4 559 kg ha-1) on a chernozem soil has been measured in case of the hybrid ECH8925. -
The effect of crop protection and agrotechnical factors on sunflower in the Hajdúság region
39-46Views:79Extreme weather conditions are becoming more and more frequent in the crop years, thus increase the risk of sunflower production.
The objective of researches into plant production is to minimize these effects as much as possible. In this sense, the optimization of
agrotechnological factors is of high importance. Within these factors, the appropriate crop technology (sowing time, crop density)
and optimized, rational crop protection technologies are important, especially in the highly sensitive sunflower cultures. The effect of
sowing time, crop density, and fungicide treatments on the yield of sunflower hybrids was analysed in different crop years in 2008
and 2009. In each case, the infection was highest with the early sowing time and at the highest crop density level (65000 ha-1). When
one fungicide treatment was applied, the rate of infection decreased compared to the control treatment. The further decrease of the
infection rate was less after the second fungicide treatment.
In the humid year of 2008 the crop yield was the highest at 45000 ha-1 crop density level in the control treatment and at 55000 crop
ha-1 crop density level when fungicides were applied. In the draughty year of 2009 the maximum yield was gained at 55000 ha-1 crop
density level in the control treatment and at 65000 crop ha-1 when fungicides were applied. In 2008 and 2009 as regards the crop
yield, the difference between the optimal and minimal crop density levels was higher in the fungicide treatments than in the control
treatment (in 2008: control: 517 kg ha-1; one application of fungicides: 865 kg ha-1; two applications of fungicides: 842 kg ha-1), (in
2009: control: 577 kg ha-1; one application of fungicides: 761 kg ha-1; two applications of fungicides: 905 kg ha-1).
In each and every case, the first treatment with fungicides was more effective than the second. In 2008, the highest yield was
obtained with the third, late sowing time in each fungicide treatment. The differences between the crop yields with different sowing
times was less than in 2009, when the results of the second treatment exceeded those of the first and third treatment in each case. -
Establishing biotic stress tolerance of maize (Zea mays L.) by measuring hydroxamic acid contents
107-112Views:181Cyclic hydroxamic acids are the most considerable secondary metabolites in grasses and their main task is to protect these species from pathogens and pests. The cyclic hydroxamic acid content and common smut susceptibility were examined in our experiments. 27 maize hybrids were used for experimental plants in a climate room, where the plants were grown on a nutrient solution. An infiltration method was used for the inoculation of the plants. The total quantity of cyclic hydroxamic acids was determined and the ratio of infected plants and the ratio of inhibition was determined, too. Based on our results, on the basis of all hybrids’ data, the total hydroxamic acid content of the infected plants was higher than in the control. On the level of individual hybrids, only 9 of them had higher cyclic hydroxamic acid content in the case of infection. Increase in cyclic hydroxamic content induced by the fungus in this case is a tool for the fungus to suppress other pathogens and pests. Amongst the hybrids’ cyclic hydroxamic acid contents, significant differences were detected in the control and in the infected treatment, too. The so-called “sweetcorn” hybrids showed high level of cyclic hydroxamic acid content. According to the differences amongst hybrids, homogenous groups were created which groups differed in the case of control and infected treatment, because of the difference in increase of cyclic hydroxamic acid content. The examined hybrids showed different levels of infection and different rate of growth inhibition for the effect of inoculation. According to the infection caused damage hybrids were ranked. Infection caused notable damage for hybrids Prelude, Desszert 73, DKC5276 and DK440.
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Testing of paraffin oil efficiency against grape powdery mildew in Eger wine region
73-80Views:355The aim of the present study was to examine the efficiency of paraffin oil against powdery mildew in Eger wine region. The experiment has been carried out in 2013 and 2014 with Chardonnay and Kékfrankos grape varieties, which have different resistance against powdery mildew. The effectiveness of the oil was examined on leaves and clusters (frequency and intensity). This oil was effective against Erysiphe necator infection on field trials in Chile and Brazil. The spread of downy mildew (Plasmopara viticola) was also inhibited by this material in some experiments conducted in Spain and France.
The differences between oil treatments represented the sensitivity of the grape varieties in accordance with the applied dosages. The oil was effective against powdery mildew with different extent as a result of the so called ”vintage effect”. In 2013, the treatment of the highest dosage (D3) didn’t differed significantly in frequency and intensity of infection from the regular treatment (clusters of Kékfrankos, leaves of Chardonnay). In 2014, the oil was not so effective against powdery mildew compared to 2013. No remarkable differences were detected between the treatments due to the strong pressure of powdery mildew. Furthermore, no any effect of the lowest dosage (D1) was detected in the case of the sensitive clusters of Chardonnay and leaves of Kékfrankos in both experimental years.
In summary, the oil treatment has an effect against powdery mildew, however this efficiency largely depends on the vintage characteristics and the pressure of powdery mildew. Further investigations are neccessary, for example field trials with combinations of other sprays. The oil can be useable as fungicide with proper care in eco-friendly integrated and bio (ecological) viticulture.
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Effect of fish feeds with high vegetable oil and low fish oil content on the growth performance and innate immune system of common carp (preliminary results)
7-12Views:76Fish meal and fish oil are very important components of fish feeds used by aquaculture. They contain poly-unsaturated fatty acids, which are essential nutrients both for fish and human nutrition. However, sources of fish meal and fish oil are limited, there is a need for new feed components to supply the increasing fish production and the increasing demand for fish feed. The aim of AQUAMAX project, supported by the European Union is to replace the fish meal and fish oil with vegetable oils. As a participant of this project, group of immunology in Research
Institute for Fisheries, Aquaculture and Irrigation (HAKI) examined the effect of three different fish feeds on the growth performance, innate immune response and resistance against Aeromonas hydrophila infection of common carp in an in vivo experiment. Fish feed supplemented with Camelina oil, but still containing fish oil significantly increased growth performance of experimental fish and their resistance against the infection. Fish feed containing linseed oil only did not enhance the growth performance, and it had a negative effect on resistance against infection. Based on the experimental results it can be concluded that fish oil used in fish feeds cannot be replaced effectively with vegetable oils, but can be supplemented with them. -
Effects of diets with different fibre content on the performance of rabbit does and on parasitological infection
41-44Views:70The effect of two diets with different fibre contents was examined on rabbit does’ performance and parasitological infection. Diet P2 had 1% higher crude fibre, ADF and starch contents than that of diet P1. The performance of 12–12 does and litters per group were examined during two consecutive inseminations. In both dietary groups, according to a 49-day reproduction rhythm, the does were artificially inseminated 18 days after kindling. Most production traits were not affected by the diets (kindling rate, weight of does and their kits, feed intake, mortality; P>0.05). Significant differences were only found in litter sizes as the number of kits was lower in P1 diet fed group at all examined days of lactation. The significant “Diet x Reproduction cycle” interaction showed that the litter sizes of dietary groups did not differ at the first examined insemination, however at the subsequent reproduction cycle 6–8% lower litter sizes were counted in P1 fed does compared to group P2 (litter size at 4 day: 9.00 vs 9.58–9.92, P = 0.004; litter size at 11 day: 8.83 vs 9.58–9.92, P = 0.037 for diet P1 at the 2nd reproductive cycle compared to all the other cases, respectively). The two diets with different compositions did not affect most of the production results of the rabbit does and their kits, but the lower litter size of does consuming P1 diet at the second examined lactation suggests the adverse effect of P1 diet’s long-term use. From the collected manure samples not any parasites were detected which presents a very favourable picture of farm from the point of view of hygiene and animal health.
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Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program
45-51Views:96In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials.
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Mitigation of environment impact of mycotoxins produced by Fusarium species
159-164Views:139The Fusarium fungi hazards the grain quality of cereals, therefore significantly affects their utilization as animal feed or consumable product. The Fusarium can decrease the quality of wheat in different ways: decreases the germination capability, causes visible discoloration, mould may appear, reduces the dry material and nutrient content of the grain, causes mycotoxin infection – as a result given by its by-product. Micotoxins produced by Fusarium genus, as the trichotecenes (T-2, HT-2, deoxynivalenol, nivalenol, diacetoxyscirpenol, Fusarenone-X) and the zearalenone (F-2) are the most common in Hungary. Occurrence of fumonisins first discovered in 1988 are must be identified carefully. About 20–30% of the overall worldwide production of cereals is infected with Fusarium and its toxins, which situation is similar in Hungary. This infection causes serious yield-losses in cereal production. In the case of cereal products, which non-utilizable as forage seems, an optimal solution is utilizing as biogas raw material, but it is also important to examine the effect of the infected cereal on the anaerobe digestion process.
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Changes of relative chlorophyll content at maize smut inoculated hybrids
55-58Views:223The leaf chlorophyll content analysis is important for several reasons. The natural or anthropogenic stressors directly effect on the chlorophyll content. Through the measurement of the chlorophyll content it is possible to obtain data concerning the physiological status of the plant, moreover the chlorophyll content is closely related to the nitrogen content, so it is linked to photosynthesis and the photosynthetic activity which determine biomass production.
One of the most common symptoms of plant diseases is the larger and smaller interveinal chlorotic areas. These might be local, or expand to the whole plant. There are multiply reasons of chlorosis such as reduction of chlorophyll content, unfavorable effects on the chlorophyll content,disorders regarding function of chloroplasts or ultimately destruction of the chloroplasts. Although such a chlorotic deviancy can contribute to significant losses in photosynthesis; however the underperformance photosynthesis of the sick plants is a more complex process.
As we unambiguously experienced during our investigations on common smut that the infected maize plants most common accompanying symptom was chlorosis on the leaves, so it is especially important to examine how the infection influenced on the chlorophyll content of different hybrids. -
Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:136Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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Sensitivity of maize hybrids to common smut under field artificial inoculation conditions
25-28Views:188Common smut disease of maize is one of the most frequent diseases of crop. In the last decades the importance of disease has decreased in feeding maize production, however its importance increasing again nowadays, especially at sweet maize hybrids. The aims of this work was to find hybrids possess of resistance, and to evaluate which ones are more or less susceptible under field artificial inoculation circumstances. Among feeding maizes the less susceptible hybrid was ‘P9578’, and the most susceptible ’NK Columbia’ hybrid, and differences in cob infection between them was significant (8.8%). At sweet corn hybrids the less susceptible was ’Prelude’, while the most susceptible was ’Jumbo’ with very high significant 74.6% differences.
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Describing Fusarium diseases on maize in 2013 using data from several production sites
60-64Views:137As in other parts of the world, the frequency of weather extremes has increased greatly in Hungary in recent years. This means that maize production is faced with greater risks from all aspects: nutrient replacement, irrigation, plant protection. This is especially true of fusarium diseases. In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. They infect the ears, which – besides reducing the yield – poses considerable risk to both human and animal health due to the mycotoxins produced by them. Depending on which Fusarium species are dominant at a given location, changes can be expected in the level of infection and in the quality deterioration caused by the mycotoxins they produce. Fusarium spp. not only damages the maize ears but when pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. The degree of infection is fundamentally determined by the resistance traits of the maize hybrids, but also a great role in that region Fusarium species composition as well.
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Varietal dependent response of barley to soil-borne Waitea circinata infection
100-106Views:155The disease syndrome caused by Waitea circinata, a soil-borne pathogen introduced in the past decade into Carpathian basin, visually indistinguishable of those caused by various Rhizoctonia strains in diverse host plant. Dicotyledonaceous species in general proved to be more tolerant to this new pathogen than monotyledonaceous ones. This mesophilic fungus can seriously damage cereals. The barley varieties, similarly to other plants, exhibited highly different individual reaction to soil borne infection, Bivoy being the most while Maresi the less tolerant among the 9 tested varieties. Two groups could be separated on the base of their response to Rhizoctonia; Jubilant, Bivoy, Pasadena formed one group being moderately tolerant and Anabell, Scarlett, Rex and Omega the other group of more susceptibles. Three significant factors influence on the virulence of Rhizoctonia strains comprised 62% of total variation.
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Lessons of a stripe rust epidemic in a wheat fungicide trial in Debrecen
35-46Views:135During the 2014 year a fungicide application trial was made as a part of technology development in Debrecen (East-Hungary). Both in this trial and across in Hungary a serious stripe rust epidemic developed and caused great yield losses. The first sympotoms were observed in April on one of trial plots and during some following weeks a serious infection grown up. On the base of 13 fungicide active ingredients and dosages by two spraying applications, their efficacy could be evaluated in stripe rust control. Picoxystrobin 250 g/L acive ingredient applied in 0.6, 0.8 and 1 L/ha dosages (Acanto), picoxystrobin 200 g/L + ciproconazole 80 g/L combined active ingredients in 0.5, 0.75, and 1 L/ha dosages (Acanto Plus), epoxiconazole 83 g/L, moreover protioconazole 125 g/L + tebuconazole 125 g/L combinations gave good efficacy, respectively. The yield of well protected plots were 5 to 8 t/ha, but the losses were 40-70% when fungicides with no sufficient efficacy in stripe rust control were applied. The great infection, which was observed on different grasses late in the Autumn, 2014 might forecast a further stripe rust epidemic for the 2015 year.
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The virus infection of South-Hungarian corn fields
52-55Views:95The past years cereal diseases, including the virus diseases have been increased in Hungary as well as worldwide. The aim of our work was to survey the virus infection of South Hungarian wheat fields. Leaf samples were collected in Szeged at the experimental farm of Cereal Research Nonprofit Co., in April and Junes of 2009 and 2010. DAS ELISA tests were carried out using Loewe antisera of Brome mosaic virus (BMV), Barley yellow dwarf virus (BYDV), Barley stripe mosaic virus (BSMV), Brome streak mosaic virus (BStMV), Wheat dwarf virus (WDV), and Wheat streak mosaic virus (WSMV) and measured with Labsystem Multiscan RC Elisa reader at 405nm. In the samples of 2009 the Wheat dwarf and Wheat streak mosaic viruses were dominated. It was also significant the appearance of the. Barley yellow dwarf virus. 2010. was favourable for the spread of the virus vectors, therefore the incidence of virus diseases increased.