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Examination of resistance against Aeromonas hydrophila infection on genetically different common carp variants (preliminary results)
7-11Views:75Septicemia disease caused by the bacterium Aeromonas hydrophila is a major problem for fish farmers around the world. In our experiment resistance to the disease was investigated on carp variants with diferent genetical backgrounds. 96 different genetic variants(„families”) were created by crossing various individuals of 4 carp strains (2 wild and 2 cultured ones). Families were reared until reaching the size of 10 gramms and 100 fish of each families were marked with an individual electronic identifier. Marked fish were divided into 5 groups. Fish from one group were infected with A. hydrophila and mortality were registered during 2 weeks. Based on mortality data, the most resistant and most susceptible families will be selected using population genetics methods.
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Some aspects of food safety from animal origin
153-155Views:111The relationship between the civilisation illnesses and incorrect nutrition can be considered as demonstrated. The functional foods having one or several nutritional- biological advantage can contribute to the healthy nutrition and to improving the quality of life. Beside the healthy nutrition it is an another important requirement that the food does not contain pathogens or substances of chemical and biological origin namely it has to be safe.
In this publication the foods of animal origin were investigated in the point of view of public health hazards arising during their production and processing.
The contamination and infection of food raw materials, the consequence of chemical substances getting in or developing it the food, the judgement of food deviring from genetically modified or cloned animals are discussed. -
Examination for Susceptibility of CAE
33-36Views:95Caprine Arthritis Encephalitis (CAE) is a group of diseases in goats caused by a retrovirus, namely the Caprine Arthritis Encephalitis Virus. CAEV belongs to the lentivirus group within the retrovirus family. The CAEV is intimately associated with white blood cells, therfore any body secretions which contain white blood cells are a potential source of virus to other goats in the flock. Once goats are infected with CAEV, it remains infected for life and has to be eliminated.
Since not all goats infected with CAEV progress to disease, very important to test goats for infection using a serology test (ELISA, AGID, IDT). These serological tests demonstrate the presence of antibodies to CAEV in goat serum or detect the virus genome in the white blood cells. However, Swiss results (Dolf and Ruff, 1994) pointed out individual and species differencies in predisposition for this disease. This result was considered as a starting point to our examination. Microsatellite analysis was used in order to find whether there was any association between genotype and serological results, and to look for a marker associated with this disease. To date, altogether 135 goats have been examined. Unfortunately, a significant association between serological results and genotype was not found using the Chi2 test -
Azoxystrobin resistance of Botrytis cinerea Pers.:Fr. isolates
56-63Views:93Fungicide resistance is one of the most important problems endangering the effectivity of practical plant protection today. The frequent and subsequent usage of specific fungicides results the emergence of resistant fungal populations. This threatens is especially high in case of Botrytis cinerea Pers.:Fr. being an endemic pathogen with frequent infection. Nowadays the main method of protection as against Botrytis cinerea is the application of chemical fungicides chemicals. Therefore, a better knowledge of local populations is necessary for the planning of the protection procedures.
Based on the results of our examinations we may establish that the growth of the examined samples showed a significant difference under in vitro circumstances, which shows a great deal of variability of the Botrytis cinerea populations in Hungary. Twenty-five Botrytis cinerea samples from different hosts were analyzed in this study. High resistance was found towards azoxistrobin in seven cases, and low resistance in eight cases.
It was also proved, that the B. cinerea is able to bypass the inhibition site of the azoxistrobin via the alternative oxidase. The presence of this altermative mitocondrial electrotransport route considerably reduces the effectivity of the chemical. -
Testing disease resistance in autumn wheat genotypes by means of field experiments
30-40Views:80According to our scientific results we can state that we have to use integrated pesticides management in crop protection against the diseases of winter wheat. One of the most important elements of IPM is to select a genotype characterised by good resistance to diseases (and by high yield ability and excellent baking quality). It is especially important that the wheat variety have tolerance against not only to one or two leaf and spike (grain) diseases, but „complex” tolerance. It is not necessary to give up the growing of a variety which has susceptibility to different diseases because we can protect it using appropriate chemical management. In the intensive growing stage of wheat (BBCH 32-37) we can use a noncompulsary fungicide-treatment (depending on e. g. the infection, ecological conditions) and, at the beginning of the flowering stage
(BBCH 59-65), we have to use a compulsary fungicide-treatment (in spite of e. g. special weather conditions, resistance genotype)to ensure high yield and good quality. -
Susceptibility of stem infected sweet corn hybrids to common smut disease
55-57Views:371The common smut of maize (corn smut, Ustilago maydis /DC/ Corda) can cause large economic losses in susceptible sweet corn hybrids as well. The protection against this pathogen is fundamentally based on prevention. Many methods to control corn smut have been recommended or evaluated, including crop rotation, sanitation, seed treatments, modification of fertility, and biological control. In spite of these frequently mentioned control strategies, the host resistance seems to be the only effective method to manage common smut in those areas where Ustilago maydis is prevalent.