The viewpoint of the recent cryopreservation techniques (CT) suggests the use of a reduced volume of cryopreservation solution, high concentration of cryoprotectants and ultra-rapid cooling and warming rates help to reduce cryo-injury and maximize the viability of the preserved animal genetic resources (AnGR). The CT had now become widely a...ccepted as one of the best methods of choice for the ex-situ conservation of AnGR due to its high success rate recorded and no-invasive nature as compared to the conventional slow rate freezing (CSRF). Rapid advances and wide acceptability of the use of assisted reproductive technologies (ART’s) particularly artificial insemination (AI) in animal breeding had resulted in a greater loss of a large number of good quality genes in virtually almost all the native breeds of animals across the globe. Small ruminant (SR) animals are not an exception in such present predicaments situation of erosion and dilution of the valuable AnGR among the native breeds. As a result of this, 148 and 16 breeds of sheep and goats respectively have already become extinct in Europe and the Caucasus. In view of the aforementioned situation, the present review aimed at exploring some of the current states of development, roles played and potentials of CT in the conservation of SR genes and genome for the immediate and future breeding purposes for sustainable development. It basically covers; animal genetic resource, the need to conserve AnGR, tools for ex situ in vitro conservation of AnGR and recent developments in breeding and cryopreservation of SR AnGR.
Cryopreservation is playing a pivotal role in ex-situ gene conservation of AnGR. Decline in genetic diversity among SR breed population was high in Europe and the Caucasus. There is therefore, need for improvent on current stringent measures on conservation of AnGR in this region of the world.
There is a mutual dependence between nature conservation activity and agriculture in Hungary, as the management of the protected areas cannot be achieved without ecological farming methods. Moreover, viable economic activity can be only imagined through the harmonization of agricultural and nature conservation interests. From a nature conservat...ion point of view, grass management systems play the greatest role in domestic agricultural systems. Yet, due to the prohibition of certain management methods and the spatial and temporal restraints on grazing, nature conservation activities have priority on protected grasslands. While nature conservation activity is still of prominent importance, it is not equally suitable for the economical management of protected grasslands per se. With our examinations, we would like to emphasize the common interests of these mutually dependent activities and to promote bilateral cooperation. Our aim is to model the production of grass on the great pastureland of Hajdúbagos. Potential grass production levels are easily calculable with a computer model based on data collected through a series of test harvests, as well as by factoring in changing climatic factors and by simulating the effects of grazing animal species and stocks. This model is not only useful for determining the optimal number of the grazing animal stock and grazing method, and therefore the most suitable management strategy, but it also supports local farmers to be able to plan their activities. In this way, both nature conservation and economic aims can be easily harmonized, which would be an important factor for the sustainable development of rural areas.
Within our experiment, we studied the combined effect of live food application and feed training on the production parameters of carp larvae. In addition, we examined whether there is a difference in the growth rate and survival rate of the specimens of Szeged mirror landrace reared in similar stocking density but with different treatments....
Specimens of Szeged mirror landrace from induced propagation were placed into 12 pieces of 40-liter aquaria. For the 4 different treatments, (K, A3, A6, A9), live food (Artemia salina) was fed, as well as feed training was applied.
We also sought to find an answer as to how our feeding and rearing conditions can affect the production parameters of common carp fries and what impact these conditions may have on their survival rates. At the end of the 2-week experiment we determined the individual weight gain of the carp fries, their survival rate and the biomass growth. The purpose of the study is to examine the optimum timing of shifting from live food to feed for carp larvae.
Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We... analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.
The effect of extended drought conditions on soil, the unfavourable cultivation technologies and the application of chemicals have been enhancing the processes of physical and biological soil degradation, so the fertility of soil is gradually declining.
The effects of two cultivation methods – traditional ploughing (TP) and conservat
chernozem soil were examined in a long term experiment. Different parameters of the biological activity of soil were determined. These are
the numbers of total bacteria, microscopic fungi, aerobic cellulose decomposing bacteria, as well as the activities of some important soil
enzymes and CO2 production.
Conservation tillage seemed to be a more favourable cultivation method for the majority of microorganisms, the activities of urease and
dehydrogenase enzymes and CO2 production, compared to the traditional ploughing system. These parameters increased significantly,
especially in the upper layer of conservation tillage plots. Concerning the plant cultures, the majority of microbiological parameters were
higher in the soil of vetch (Vicia sativa L.) depending on the cultivation methods, so involving the pulses to the crop-rotation seems to be
very important in this soil type.
According to the ninth year’s results, the importance of conservation tillage as a means of protecting the soil biological activity in meadow
chernozem (Vertisols) can be established; it was proven by microbiological investigations.
During our research we aimed at finding an answer as to what extent the different concentrations of 17-alpha methyl testosterone incorporated in the diet of common carp fries can influence the production parameters of the species, as well as how efficient their sexreversal can be with the use of this method. To this end, an aquarium experiment...was conducted in the course of which four different hormone treatments were set and monitored. The fish feed was enriched with 17-alpha methyl testosterone in 50 ppm, 75 ppm, 100 ppm, 500 ppm dosages.
The obtained figures revealed that the hormone treatments had no influence on the production parameters and conservation of the common carp fries. Further on, our team is to determine the sex of the fish through the examination of gonads during autopsy when they reach the 500 g average weight.
Furthermore, a male specific test method which was supposed to be of great help in our attempt to select the sex-reversed specimens in the subsequent processes was also put to the trial. During the experiment the DNA-isolation of different sample types (muscle tissue, fin, mucus) of common carp with identified sex was successfully carried out. The extracted PCR product was examined with agarose gel. Our results indicated that the ccmf2 marker was applicable, however, the obtained figures were not reliable.
Aquaponics is an integrated system that combines fish farming (aquaculture) and hydroponic plant production. The objective of this study was to examine how the level of dissolved oxygen with or without an air pump affects water quality, fish output and plant growth parameters for common carp (Cyprinus carpio) and basil (Ocimum... basilicum).
Ebb – and flood aquaponics systems (with automatic syphon) was used. Two treatments were set in this experiment, one of which was the aquaponics system without air pump (unit I), where water of the plant bed was pumped two directions, one falling back to the fish tank oxygenating the water the other was pumped to the hydroponics unit. The other system (unit II) was designed with an air pump.
In the course of the study, water quality parameters, such as oxygen saturation, dissolved oxygen (DO), electrical conductivity (EC) and nitrite were significantly different (p<0.05). Total basil biomass was higher in unit II. (5367.41 g). The final biomass of common carp were
2829.45 g ± 79.24 and 2980.6 g ± 64.13 g in unit I and unit II respectively. Weight gain (WG) and specific growth rate (SGR) showed no significant differences (p>0.05) between the treatments.
Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression... from the same sample to correct for technical related variations).
Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments.
In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to im...prove immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.
...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.
Our botanical survey at the great pasture of Hajdúbagos is a part of a broad research that aims to predict the production of the grass at the given area. As the mentioned pasture is a nature conservation area, the usage of artificial fertilizers or other classic grassland management methods in its handling are prohibited. Thus grazing is an im...portant tool for the management of this area, however the not suitably regulated grazing order and the poorly calculated carrying capacity cause serious problems at some parts of the pasture. The prediction of the grass yield is essential to
avoid both over- and both under-grazing and for determining the optimal number of the grazing animal stock and the grazing method, thus the most suitable management strategy.
The potential grass yield is easily calculable with a computer model that will be established as a basis for determining the grass production. For the sake of getting an accurate view of the plant associations of the pasture, we created examination quadrates and determined all plant species found in the quadrates. After plant determination, we compiled a coenological table in which we marked besides the scientific name and families, the life forms of each species that refer to the structure, morphology and thus the adaptability of plants to their environment. We determined the
TWR, so the thermoclimate, water and soil reaction values, the nature conservation values, as well as the covering values of each plant species (DB), and the total coverage of the examination quadrates (B%).
According to the covering values, grasses proved to be characteristic plants at the examined pasture, thus we need to consider them influential in calculating the animal carrying capacity and with the rest of the information, we need to supply the model.
The life forms and TWR indicators, all together with the nature conservation values provide further important data to the development of the management suggestion of the protected pasture. By examining these values to different parts of the area, we could get an exact view on the measure of the degradation effects. This promotes the determination of grazing methods and the forming of the boundaries of certain pasture sections, to avoid those harmful anthropogenic effects that seriously endanger this extensive sandy pasture.
Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, an ecologically sustainable horticultural production technique with long traditions.
The objective of this study is to compare flood-and- drain, and the water crossflow system and examine the differences in the water quality, fish yi...eld and plant growth parameters for Common carp (Cyprinus carpio) and basil (Ocimum basilicum). During the study, water quality parameters of two treatments were compared in temperatures, pH, EC and NO3¯N were significantly different (p <0.05). Leaf area of the basil plants grew to an average of 20.37 cm2 (± 9.02 cm2). The plants’ biomass production was significantly different (p< 0.05) in the two systems. The biomass production showed lower yield, 458.22 g (± 214.59 g) in the constant flow system that in the flood- and- drain system 692.9 g (± 175.82 g). Fish Growth parameters were better in constant flow system (FCR 5.48 g/g ± 0.19). However, the specific growth rate (SGR) demonstrated that fish grew faster in flood- and- drain system 1.38 %/day (± 0.29).
This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oli...gosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.
In the past decade, the population of the Hooded Crow has shown significant growth in Debrecen, Hungary. The aim of this study was to become acquainted with the nest-site selection behavior of Hooded Crows in urban spaces. While our research revealed that the Hooded Crow is not particular when it comes to selecting among tree species for nestin...g, we did notice differences regarding nesting height. Hooded Crows living in the city build their nests higher up than those living outside Debrecen’s city limits. We also discovered a slight, insignificant difference between nesting heights and tree species, which is probably due to the different characteristics of the given tree species. As for nesting heights, we found that in typical urban habitats there were no relevant differences. However, when we compared these habitats in pairs, it came to light that nesting heights -when comparing solitary trees - wood segments and tree rows - wooded segments- did show significant differences, which can be explained by the various conditions provided by the habitats mentioned.
In summary, the following results emerged from our research:
1. The Hooded Crow prefers approximately the same nesting heights in all kinds of habitats, urban environment and tree species.
2. The nesting height does not significantly depend on the habitat itself or on the tree species.
Population genetic indicators of the Hungarian Hucul population were calculated taking also into account the effect of imported horses.The birth year of the examined 3002 individuals ranged between 1871–2015. The last year involved in the examination was 2015, when the research was conducted on the stock under stud book control. The calculati...ons on the population were made using the statistic indicators in the Endog programme, with and without the imported Polish horses. Figures were depicted using R software. Number of offspring of the Polish individuals was presented in tables; the distribution of the offspring per stud and per mare family, as well as the Nei’s genetic distance,were presented graphically.
Modern animal husbandry has drastically changed the genetic structure of some domestic species. The varieties, genotypes that we think we do not need at the moment can only be saved from extinction with the help of gene conservation. Traditional Hungarian horse breeds have a long history (more than 200 years) and a demonstrably different ge...netic structure from other horse breeds in Europe and the rest of the world. Consequently, their enormous genetic value is undoubted. The subject of our research was to study the structure of the mare families found in the population of the Lipizzan horse breed and the genetic structure of the Hungarian population. Out of the total 61 mare families in the world, 35 are present in Hungary. There are 11 Hungarian, 12 families of Fogaras, 7 original, 4 Croatian and 1 Slovenian mare families in the Hungarian population. The proportion of mare families in Hungary and Fogaras is almost the same. The proportion of Croatian and Slovenian families is negligible. In terms of their number, they are not significant in Hungary. For this reason they have little effect on the Hungarian Lipizzan population. Except one of the original mare families are in the same situation as the Croatian and Slovenian families. The Presciana / Bradamanta mare family is the most populous of all families due to their long stay in Hungary. The proportion of families is unbalanced. Seven mare families accounting for 56.36% of the total population. Given the basic requirements of gene conservation work, this condition is far from optimal.
We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full... generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.
The aim of this research was to prove that the syrup containing ragweed used for feeding the bees was transferred to the honey. To reach this goal we developed a method to analyse the volatile components of the samples. We applied two sample preparation methods. The first one was SPME (Solid Phase Micro Extraction) preparation from the headspac...e of all of the samples. In the second case only the raw leaves of the ragweed were extracted with n-hexane and dichloromethane to complete the SPME results. The measurements were performed on GC-MS equipment. Chromatograms and data evolution showed that the components of the ragweed were also present in honey so they were transferred by bees.
The nature conservation park, which belongs to the Spa and St. Andrew Hospital for Rheumatics of Hévíz, is 60 ha in size. The famous Lake of Hévíz, the two overfalls and the main buildings of the hospital are located in the middle of the park. They are surrounded by protective forests, parks and gravel esplanades. During the past few decade...s, the population of neophyton plants and invasive insects have increased considerably. These mean serious challanges to develop efficient control methods. Special care must be taken of environmental and plant protection regulations. Keeping plant protective regulations are especially strict around natural and spa waters.
The goal of research planning is to optimize human and material resources while maximizing efficiency. If there are databases that can be used as a substitute for own data collection, effective research can be facilitated by reallocating resources. In the case of environmental studies, the knowledge of the climatic conditions of the study p...eriod is known to be a key factor in research planning process.
In the present study, the data of our meteorological station in our research area (known as “Szamárháti Tanya”, Kesznyéten, Hungary) was compared with the measurements of meteorological stations operated by the competent water authority. Stations were taken into the study within a 10 and 20 km radius over a period of 21 months, to determine which provided more relevant data from the area.
During the evaluation of results, the relationship between the local and regional weather stations were determined, and deductions were made from the obtained results to support the decision which of the targeted investigations could be of greater benefit.
Chromatometric examination of the plumage of birds is a poorly researched topic. We have approached this issue in primarily aspect of differences in plumage of species. Moulted feathers sample collection method has been increasingly used. Reliable identification of feathers becomes an increasingly important issue, hence need for an exact measur...ement-based methodology. Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) primary, secondary and tail feathers were studied. Chromatometric parameters of feathers were measured in CIELAB color system and then statistical analysis (Independent samples t-test, Descriptive Statistics, Discriminant Analysis) was performed to compare the two species. Instrumental measurements has been confirmed the high similarity between colors of the two species, however species specific differences were also found. Lightless (L*) value were significantly characteristic of particular species, while the red/green (a*) and yellow/blue (b*) value had lower Predictive Power. We identified feathers and the variables which useable to separate the two species and determined the associated Confidence Intervals of these values. Our results may draw attention to a new potential direction for exact identification of the moulted feathers during sample collection.
The Eurasian Collared Dove shows different patterns in density all over Hungary. In some cases, the population has decreased to a lower level, but there are habitats where the population remained stable. Several factors were taken into consideration during the evaluation of this phenomenon. These publications state that the increased urbanizati...on of corvids, the lack of food sources, and the changes in nesting sites caused the decrease in numbers. During our research, we intend to contrast the differences in population structure of Collared Doves that differ from each other in the density of predators presented at nesting sites. As a first step, in this paper, numerous samples (n=118) were taken from a population that is less influenced by corvid species’ nest predation. Our prediction was that the population parameters remained at the level of 1980’s, in which the population of Eurasian Collared Dove has reached its maximum, according to further publications.
During the evaluation process, our data were collected from hunting bags near Nyíregyháza. Results were compared with former publications. We stated that the population structure, such as age and gender distribution remained stable in the evaluated population. In addition, morphometric factors of the collected individuals were also measured, such as weight, wing length, 3rd primary feather, and tail length, which were compared with former surveys. During statistical tests no significant difference was revealed among population parameters, but there were significant differences between most of the morphometric data, (p>0.05).
The common carp (Cyprinus carpio L.) is the most important fish species in Hungary, it is more than 70% of the total Hungarian fish production. The common carp production is important not only just in Hungary but in Middle-East Europe, as well as Southeast Asia. Majority of the production comes from fishpond culture. If the production secto...r wants to meet the increasing customer demands, there is need to intensify research on the intensive fish production opportunities for example all-female common carp technologies. The all-female technology is one of a genom-manipulation technology. Its production showed better growth rate than mixed-sex population in pond culture. Our experiment combined the recirculation aquaculture system (RAS) and the all-female common carp stocks intensification technologies. The reason for the experiment, is that there are no result about the growth of all-female common carp growth in RAS.
The experiment used the „Tatai grey scale type” common carp stocks. We propagated two all-female stocks (T2 and T3) and a control group (TK). Due to technological characteristics of RAS, the water quality parameters were the same for all treatments and corresponded to the technological tolerance of common carp.
The experimental period was from July 10, 2019 – November 20, 2019. Result of growth performance showed that the growth of mixed-sex stock was significantly higher than all-female stocks; (Control=3692.0±590.5g, T2=3438.8±415.4g, T3=3294.1±659.1g). Feed conversation ratio (Control=1.3±0.1 T2=1.5±0.2 T3=1.6±0.5) and SGR% (Control=0.8±0.0 T2=0.7±0.1 T3=0.7±0.1) were similar.
By the results it can be said the all-female common carp technology has neither advantages nor disadvantages compared to the mixed-sex stock. The all-female technology can be beneficial if the consumers need female common carps. It is worth continuing the experiment and examine how the stocks will perform above 3kg body weight.
The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of informati...on on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.