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Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:238Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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Germination and sugar content alteration in maize grain caused by Fusarium contamination
42-44Views:130Healthy and Fusarium affected ears were collected in Fészerlak, Somogy County at the end of vegetation cycle of maize (25-30 August). Each pattern contains 25-25 ears. We compared the samples on the basis of visual image of Fusarium affection. The ears were shelled and two 0,5 kg samples were formed: healthy and Fusarium contaminated. After surface sterilisation the uniform sized seeds were soaked in sterile distilled water for 24 hours and there were germinated for 7 days. The α-amylase activity was measured with Phadebas- α-amylase test. Seeds were extracted one by one three times under reflux using 10 cm3 boiling water for 15 minutes. During our investigation germinating activity was detected to measure glucose, fructose, sucrose content and α-amylase activity. In the first seven days of germination the highest values were detected in control seeds followed by the affected seeds. Our results clearly show that stress conditions applied altered not only the saccharide content but decreased their germinating activity as well in the case of maize grain.
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Assessment of electrical conductivity and germinability of groundnut genotype seeds
5-10Views:497Seed quality affects crop establishment and productivity. In addition, the use of good-quality seed is an essential prerequisite for sustainable crop production including groundnuts. Assessing germinability and electrical conductivity provides early evidence of the production potential of a given crop variety or genotype. Therefore, this study assessed the germinability and electrical conductivity of seeds of three groundnut genotypes. A laboratory experiment arranged in a Completely Randomized Design (CRD), replicated three times, was conducted at the Faculty of Agriculture, Kyambogo University, in 2020. Seeds of Igola, Serenut 1, and Serenut 2 groundnut varieties were tested, and data was collected on germination percentage and electrical conductivity. Analysis of variance (ANOVA) was performed using GenStat and means were separated using the least significant difference test at a 5% probability level. Germination percentage and electrical conductivity significantly (p<0.05) differed among the groundnut varieties, with Igola recording the highest germination percentage, followed by Serenut 1, and the lowest was in Serenut 2. The highest electrical conductivity was recorded in Serenut 1 and the lowest in Igola. Since Igola had one of the lowest electrical conductivity and the highest germination percentage, it was concluded that Igola genotype retained higher quality attributes.
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The influence of crop rotation and nutrition regime in winter wheat on the phytomass remains and the deposits of weed seeds remained in the ground
10-13Views:195An important problem refers to soil weeds seed bank with special reference to brown luvic soils and relationship between vegetal remains
(roots + stub) and nutrition regime – crop rotation plant. A great number of seeds can be found in first 20 centimeters of soil (plowing
layer). This fact causes a high weeds density, which has a negative effect on the cultivated plants leading to partial or total crop loss. -
Effect of sowing time and planting space on change of yield and protein content of white lupine (Lupinus albus L.)
85-89Views:258The hinge of the lupine crop cultivation technology is the sowing time and the determination of the breeding area. According to the literature the early sowing (as soon as possible to go to the soil) and larger growing area is recommended for seed production. Based on the results of our 2 years experiment, the effect of sowing time and different spacing is important on yield of white lupine, and the effect of the sowing time is more significant. A decrease in the yield was observed after later sowings. The yield and the rate of its decreasing was varied in one year and among different years depending on wheather condition and sowing time.
The role of sowing time is significant for protein content of seeds. The average protein content decreased due to the later sowings in 2014, while 2015 the protein content of seeds in early sowing time and of plants sowed 4 weeks later was similar. We measured less protein content in the 2. sowing time sowed 2 weeks later than the first one. In the studied years the average protein content of seeds from different row spaces were not different.
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Study of drought stress correlation on yield and yield components of maize cultivars (Zea mays L.)
67-73Views:514This article was investigated to study the correlation and analysis of drought stress regression on maize cultivars' yield and components. The variance analysis results showed a significant difference between drought stress levels in terms of plant height, total dry weight and number of seeds per row, the total weight of cob, grain yield, harvest index, stem diameter, and cob weight with protective leave. Also, there was a significant difference in ear weight without protective leaves, ear diameter, ear length, plant weight, 100-seed weight, and seed per ear on hybrid treatments. There were statistically significant differences between cultivars in plant height, leaf area, ear diameter, ear length, number of seeds per row, number of seeds per ear, the total weight of cob wood, 100-seed weight, harvest index, plant dry weight. The results of the correlation of traits for the mean levels of drought stress showed a positive and significant correlation between plant yield and plant height, seed per row, ear length and weight of 5 pieces of wood and also with a total weight of cob wood, ear weight with bark showed the highest correlation. There is a significant correlation between leaf area and stem diameter, plant weight, total dry weight at the probability level of 0.05. Correlation coefficients between traits in non-stress conditions showed a positive and significant correlation between grain yield and height, ear length and grain in the row, which was a significant increase compared to stress conditions. The correlation of traits under full stress conditions also showed that the correlation coefficient between cob length trait and positive height was positive and significant. From the study of correlation coefficients between maize traits in non-stress conditions, it can be concluded that the most important components of grain yield are cob length and grain per row. While the correlation coefficients under moisture stress conditions show that the grain trait in the row has a positive and significant correlation with yield, under stress conditions in the cob stage did not show any traits with correlation yield.
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Studying the effects of traits in the genotype of three maize hybrids in Hungary
97-101Views:766In this study, we selected three hybrids (Armagnac, Loupiac, and Sushi) for evaluation of the effect of traits in genotype in Debrecen. In 2017, the total rainfall from May to October was 314 mm in Debrecen, which was 236 mm in the winter period before sowing. The obtained results showed that there was a positive correlation between the weight of the cob maize and the rate of seed/cob, number of rows with number of seeds in column and outer diameter ear with weight of cob and number of rows in grain per ear and the rate of seed / cob; Also, there was a negative correlation between grain weight in ear with seed/cob rate, outer diameter ear and the rate of seed/cob and outer diameter ear with the number of leaves. There was a positive correlation between stem diameter, Seed/cob rate and the number of nodes by GGE biplot. In addition, there are traits of weight of all seeds and outer ear diameter that had the highest effect on average yield. Moreover, the number of seeds per row showed the least effect on the average yield of hybrids.
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Application of GIS, precision agriculture and unplugging cultivation in plant breeding of Karcag
49-56Views:309In the last two decades, the prevailing ecological conditions and climate change have caused negative effects. Therefore, a paradigm shift is needed in the field growing of plants. The latest inventions, digital technologies, precision cultivation are not enough, the mentality of the farmers is more important. For this reason, not only big financial sacrifices, but adequate receptivity are needed on behalf of farmers. Adequate skills and continuous self-education are necessary. The yield of plant growing farms is determined by ecological conditions to a 40% extent, genetic background of the seed has a 30% share and the applied agricultural technology has a proportion of 30%. In different agroecological conditions, bred varieties of plants have bigger tolerance to unfavorable factors of the regions and significant yield stability. Farmers, who buy and sow seeds, can only contribute to the genetic potential of the seeds with cultivation technology. Plant breeding provides stable genetic background and good quality seeds. Breeding activity – choosing variety proposants, breeding them, selection work, classical breeding process for 8-10 years – must create new landraces, which can produce balance, high yield and have good quality parameters in extreme ecological conditions, yearly excursion and have higher tolerance to unfavorable factors of the region giving significant production stability for farmers. In Karcag GIS technology, precision cultivation elements and soil-friendly agrotechnical methods have been introduced which largely support the aims of breeding and can also provide optimal cultivation conditions in extreme years. Because of the specificity of breeding the main aim is not only to increase yield but to provide harmonic growing for bred materials, to decrease the number and the cost of cultivation and to be punctual. In this study, applied new methods and technologies are introduced.
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Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times
5-12Views:306Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.
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Occurrence of woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) in Hajdú-Bihar county, Hungary
119-123Views:160Woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) is native to East Asia, it spreads in several parts of the World and causes difficulties in plant protection, especially in maize. Difficulties in control of Eriochloa villosa originated from several reasons: seeds continue to germinate later in the season, significant part of seeds emerges from a deep layer of the soil, and the species is less susceptible to some herbicides applied to maize than other annual grass weeds.
The first report on the occurrence of woolly cupgrass in Hungary was published in 2008, and it reported about the appearance of this species near to Gesztely village (Borsod-Abaúj-Zemplén county), however, no information has been added about spread of the weed in Hungary until now.
A significant population was discovered next to Debrecen (Hajdú-Bihar county) in summer, 2011, and then weed associations were examined in maize, sunflower and stubble-fields on several km2 in the area to estimate the Eriochloa villosa infection. The weed species was found on every maize field bordering with a ground cover of 0.5-4%. Woolly cupgrass occurred inside of the 50% of maize fields, and reached a ground cover of 76% in case of most infected area, in addition it was found in sunflower and stubble-fields.
The spread of woolly cupgrass is expected in this area, which requires the consideration of this species in the planning of weed management technologies. -
Exogenous salicylic acid treatments enhance tolerance to salinity of wheat (Triticum aestivum) plantlets
34-38Views:204Salt stress, an abiotic stress, determines modifications of some biochemical indicators, like, antioxidant enzymes, proline (amino acid
accumulate in higher plants under salinity stress) content, and some physiological processes including: plant growth and development. In
this paper we studied the influence of exogenous treatment of wheat seeds, with 0.1 mM salicylic acid (SA) solution, in the plant response to
salt stress. The treatment was applied by presoaking the seeds in the treatment solution for 12 hours before germination. The results showed
that exogenous 0.1 mM SA solution, administrated to the wheat cariopses significantly ameliorated the negative effect of salt stress in first
week of germination in laboratory conditions. -
Economics of site specific crop density in precision sunflower (Helianthus annuus L.) production
91-96Views:371In this research, the crop density of sunflower was examined, which, thanks to the tools available for precision crop production and knowledge of the market environment of sunflower production, best fits the heterogeneous areas of the given production zones and meets the economic requirements. These components together directly influence the effectiveness of sunflower production. In the year of 2021 and 2022, we carried out a site-specific crop density sunflower experiment in two fields with the same soil type, by sowing significantly different amounts of seeds within the given zones. We have established that the sunflower, although a plant with excellent adaptability, reacts sensitively to the place of production and the effect of the year, in zones with heterogeneous productivity, and shows a reaction to sowing with a variable number of seeds per zone, even when examined based on economic aspects.
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Examination of the usability of the MALDI-TOF method in sunflower genetic identity analyses
33-38Views:308Economically, one of our most important crop is the sunflower. Regarding its production it is essential to use top quality seeds. As the conditions of seed certification and the certification parameters are regulated by laws and ordinances, it is highly important to farmers and seed producers to detect seeds of low quality or dubious origins.
Nowadays, the examination of the cultivar homogenity of sunflower is based on a reference method of the International Seed Testing Association Rules International (ISTA), Chapter 8. This standard reference method uses ultrathin poliakrilamid isoelectric focusing gel electrophoresis (IEF-UTL, or simply IEF). With this work we have set out to compare the results of MALDI-TOF to the test results – used as reference results – achieved by this reference method. The aim is to develop a new, quick, cheap and reliable method. In this article I summarized the results of some of the experiments that will provide basis for my further work.
During our previous experiments we have concluded that out of four extracting agents we can get the most protein markers using NaCl acid buffer, 1 propanol buffer. -
The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)
39-44Views:477Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.
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Behavior of some Hungarian wheat varieties to seed soaking in gibberellic acid under salt stress
7-17Views:350Various abiotic stresses including high salinity strongly affect seed germination. Three Hungarian wheat landraces Gamási, Fóti and Kartali have been tested for seed germination, seedling growth and chemicals parameters when seeds were soaked in gibberellic acid (GA3) at the concentrations of 0, 75 and 150 ppm and at four levels of salinity stress 0, 5, 10 and 15 dSm-1. A laboratory experiment took place at Research Institute of Nyíregyháza. Factorial Experiment based on Randomized Complete Block Design (RCBD) in four replications has been conducted. Seed soaking in GA3 before sowing significantly affected germination characteristics, seedling properties and the results of chemical analysis. The highest germination as well as K+ content of seeds were observed at seed soaking into 150 ppm GA3. However, Na+ and proline contents in seedling were decreased. The behavior of the tested Hungarian wheat varieties was genotype-dependent. Increasing salinity stress up to 15 dSm-1 significantly affected germination characteristics, seedlings parameters and chemical analysis. Fóti variety turned out to be the best at 150 ppm GA3 compared to the other two varieties. Moreover, it had the lowest Na+ and proline contents and highest K+ content.
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Effects of different crop years and sowing date on maize yield
93-96Views:276We carried out the tests in the flood meadow soil formed on the alluvial cone of Nagykereki, Sebes-Körös belonging to the Bihar plane small region. The aim of the study was to analyse the effect of the different sowing date of maize on the yield trend based on a comprehensive study conducted for 6 years (2007–2012).
The sowing date of maize hybrids is a factor that significantly influences yield, however, its effect is not significant in each crop year. In the years when the date of sowing has a modifying effect, the reliable yield level can be reached with optimal sowing date management (24 April).
The advantage of early sowing (10 April) proved to be dominant in the year of 2012, the seeds were placed into the still wet soil therefore shooting was more balanced. Maize seeds sown at the time of optimal (24 April) and late (10 May) sowing dates were placed into the already dry soil, which deteriorated germination and the strength of early initial development that had an effect on the yield.
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Correlations study between the selenium content of wheat grass (Triticum aestivum L.) and wheat seeds grown on different soil types
117-121Views:257In the course of the research we determined selenium and dry-matter content of 35 wheat grasses and 35 wheat seeds. The selenium content of the preparation plant samples was measured by spectrofluorimetric determination (ʎexcitation=380 nm, ʎemission=519 nm) of the resulted piaz-selenol complex. It was established that between the selenium content of the wheat grass and wheat seed the correlation coefficient was 0.36 at p=0.05 level which indicates a medium close correlation. Similarly, there was a medium close correlation between selenium content of the wheat grass calculated on dry-matter basis and total selenium content of the wheat, with a correlation coefficient of 0.40 at p=0.02 level.
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Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:194We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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Utilization of Okra (Abelmoschus esculentus L. Moench.)
122-125Views:202Okra is warm season crop native to Africa. Presently it is widely grown in the northeastern parts of Brazilia, both northen, and western parts of Africa, southeast Asia, and in the eastern parts of the Mediterraneum. In Hungary it is a rarity. It’s adaptation to Hungary began about 40 years ago.The optimal sowing time under Hungarian climatic conditions is middle of May. The harvests is started – depending on the weather – first, by the middle of July, and picking continues until the first of September.
According to our results, seed soaking, sowing under a perforated foil sheet-covered trench (2 weeks earlier the normal sowing time), and 55-65 g thousand weight seeds are favourable.
The harvest period under Hungarian circumstances is appr. one and half months. -
Alternative protein sources in the nutrition of farm animals
21-31Views:608Protein requirement and its demand of farm animals became one of the critical problems in nutrition on a global scale. Protein requirement has been an explicit demand for a long period with soybean meal and animal protein, but recently there are some limitations in relation to their use and the availability of the high quality fishmeal decreases constantly. For this reason there is increased demand for finding new protein sources which could be the alternatives of soybean meal and fishmeal. Alternative protein sources can be divided into seven categories, according to their origin. In different countries, their use depends on the availability in large quantity and at reasonable price.
There is a long tradition of using legume seeds, as alternatives of soybean. Most of them contain some anti-nutritive compounds, but it can be reduced with systematic selection. Oilseed meals are also generally use in poultry and pig nutrition, but those crude protein content varied, depending on the oil extraction technology. Green fodder and leaf protein was also proposed as alternative protein sources, but their use is limited, in particular because of the market price. The amount of bioethanol and starch industry by-products increases gradually in recent years, therefore those became alternatives of soybean meal, or in much less extend, fishmeal. However, amino acid composition of such by-products are far from optimal for poultry and pig; therefore, in the case of their use amino acid supplementation is necessary. Several novel protein sources are proposed in the last decade, such as algae or insect proteins. Recently, their availability and use is limited, but in the near future those would be alternative protein sources in monogastric animal nutrition.
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Statistical comparison of soil analysing results of chernozem soils
93-99Views:311The soil fertility was degraded as a result of unreasonable tillage, therefore the application of site-specific nutrient replacement is necessary. It is essential for the application of precision fertilization to know the location, extension, soil properties and nutrient-supply of the different soil types of
cultivated areas.
We collected soil samples from 580 hectares of land in 2006. Soil samples were collected from every 5 ha in 30 and 60 cm depths during Spring from 20.05.2006 to 12.06.2006 and again in Autumn from 09.19.2006 to 02.10.2006. Soil samples were analysed at the Department of Agricultural Chemistry and Soil Science of DE-ATC.
The two examined soils are slightly calcareous, weakly saline, poor in zinc. The calcareous chernozem soil is slightly acid, the content of humus, nitrogen, phosphorus and potassium is medium in this soil. The meadow chernozem soil is slightly alkaline, and properly supplied with humus and potassium, and middling supplied with nitrogen and phosphorus. The meadow chernozem soil is more heterogenous in soil plasticity, lime, saline, nitrogen phosphorus and potassium content and less heterogenous in pH and zinc content than the calcareous chernozem soil.
Standard deviation of measured values in pH, soil plasticity, humus and nitrogen content significantly differ between the examined soil types. The soil plasticity, pH, humus, nitrogen and zinc content significantly differ among calcareous chernozem soil and meadow chernozem soil, but the difference in phosphorus content can be statistically proven only in case of Spring soil sampling. -
Presence, feeding and health status of collared doves (Streptopelia decaocto Friv. 1838) on animal breeding farms
49-54Views:253In the course of our examinations we were determining the gender of collared doves (Streptopelia decaocto) feeding at animal breeding farms by biometric data. We were estimated their nourishment and necropsies were carried out.
We compared the results of the nutrition analysis with similar data of collard doves feeding at agricultural areas.
We analyzed the crop and gizzard content and recorded the body proportions of collard doves that were captured between December of 2004 and August 2005. In the course of the necropsy examinations we did parasitological, bacteriological examinations as well as examinations for the detection of Newcastle disease. In the course of the gender analysis of the collard doves that were collected at animal breeding farms, both the wing length and the length of the third quill-feather has shown a significantly greater value in the case of males (p>0.05).
From the orts found in the crop and gizzard it has been stated that the feed consumed the most was corn (80.77% m%). The collared doves consumed much less other cereal seeds (wheat 0.82%, barley 1.26%).
In the course of the necropsy examinations done in the Veterinary Institute of Debrecen, the birds proved to be free of parasites, and the results of the Salmonella and Newcastle disease analysis were negative as well. E. coli bacteria had been detected in the liver of not more than three birds. -
Evaluation of chickpea (Cicer arietinum L.) in response to salinity stress
105-110Views:682Soil salinity is a severe and expanding soil degradation problem that affects 80 million ha of arable lands globally. Chickpea (Cicer arietinum L.) is very sensitive to saline conditions; the most susceptible genotypes may die in just 25 mM NaCl in hydroponics. Approximately 8–10% yield loss in chickpea production is estimated due to salinity stress. However, it is still not established why chickpea is so susceptible to salt affection. Salinity (NaCl) impedes germination of seeds, though chickpea varieties considerably differ from one another in this respect. Some chickpea genotypes are more tolerant in the stage of germination, tolerating even 320 mM NaCl. The reasons of this variation are unrevealed; there is a shortage of knowledge about the germination abilities of chickpea genotypes in saline conditions. Nevertheless, the effect of salt stress on vegetative growth can be analysed in hydroponics, in pot or field conditions, regardless the experimental environment, the ranking of genotypes regarding salt resistance is coherent. Chickpea genotypes can be different in their ability to retain water, maybe under salt affection; the more salt tolerant lines can maintain higher water content in the shoots, while the more sensitive ones cannot. The identification of salt tolerant chickpea landraces based on developing genetic variability is a suitable strategy to combat against salinity problems arising in arid and semi-arid areas.
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Fruit melanotic ringspot (FMRS) – a disease of resistant Capsicum genotypes infected with Tomato spotted wilt virus (TSWV) on the fruits
64-69Views:145Etiology of pepper fruit melanotic ringspot (FMRS) disease (Salamon, 2009) was studied on fruit samples collected in forced pepper populations. It was noticed that in spite of heavy thrips (Frankliniella occidentalis) infestations and of TSWV epidemy detected in the forcing houses, FMRS occurred only in plants having healthy foliage. Symptomatological surveys strongly suggested that FMRS appeared exclusively in specific pepper genotypes. The size of melanotic ringspots has been observed to grow at room temperature during postripening of diseased fruits. A mechanically transmitted plant virus was isolated from symptomatic parts of 9 white pepper fruits affected by FMRS. On test plants each of the virus isolates caused systemic symptoms characteristic to TSWV. Using cDNA/PCR technique and TSWV N-gene specific primers a ca. 300 bp long DNA fragment has been amplified from total nucleic acid extracted from symptomatic tissues but never from asymptomatic parts of the fruits showing FMRS. Plant progenies grown from seeds of FMRS diseased fruits segregated in respect of resistance and/or susceptibility to TSWV infection. TSWV was also detected in and isolated from three fruits showed non-melanotic yellow rings (one of them was infected with a tobamovirus, too). Seedlings derived from these fruits proved to be susceptible to TSWV. Based on the above results we could conclude that the FMRS disease developed on fruits of “cecei” type white peppers that carry a TSWV resistance gene, most likely the Tsw gene in heterozygous form. These fruits were infected with thrips transmitted TSWV and FRMS appeared as a hypersentive reaction (HR) manifested in fruits.
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Application of AFLP-Method in Plant Sample Identification
207-213Views:237One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.