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Impact of ammonium nitrate and Microbion UNC bacterial fertilizer on dry matter accumulation of ryegrass (Lolium perenne L.)
35-39Views:101Pot experiment was performed to investigate the effects of increasing NH4NO3 doses with or without Microbion UNC bacterial fertilizer
application on dry matter production of ryegrass (Lolium perenne L.). Experiment was set up on calcareous chernozem soil of Debrecen-Látókép and on humus sandy soil of Őrbottyán. The bi-factorial trials were arranged in a randomized complete block design with four replications. Grass was cut three times. Dry matter production was determined and the sum of biomass of cuts was calculated as cumulated dry weights. Analysis of variance was carried out on the data in order to provide a statistical comparison between the treatment means. The least significant difference (LSD5%) test was used to detect differences between means. On the basis of our results it can be concluded, that the dry weights of ryegrass cultivated on chernozem soil were higher than on sandy soil. With increasing nitrogen supply the dry matter production of grass significantly increased in both types of soils. In case of sandy soil the increasing effect was more expressed, but dry weights of this soil never reached the appropriate values of chernozem soil. Application of Microbion UNC had positive effect on dry matter production of ryegrass grown on both two types of soils but the effect was more expressed on chernozem soil. Finally it can be concluded that the increasing effect of NH4NO3 on biomass weights was more expressed in both types of soils, the biofertilizer application also increased the dry weights of plant in a small degree. -
Sensitivity study on Virginia fanpetals (Sida hermaphrodita (L.) RUSBY) cv PETEMI to different herbicide agents
89-92Views:86The cultivation of Virginia fanpetals (Sida hermaphrodita (L.) Rusby) is a relatively new phenomenon in Europe. On the basis of the biology of the plant and the practical work implemented, it has been stated that the traditional field practice does not provide the appropriate conditions of biomass production for energetic purpose. The development of the proper weed control is inevitable for the healthy stand, as in the early phenophases the plant is growing slowly and it is exposed to weeds.
Our objective was to test some herbicide agents as no previous relevant data had been published. -
Potential use of bamboo in the phytoremediation in of heavy metals: A review
91-97Views:357There are many literature sources focusing on the phytoremediation of woody plants, but there are only few dealing with the phytoremediation of bamboo plants. Phytoremediation technology has the advantages of little disturbance to the environment and low remediation cost. Bamboo mainly exists in tropical and subtropical regions. As an energy plant, bamboo has a fast growth cycle, large biomass, simple cultivation, high economic efficiency, and convenient harvesting, which highlights the advantages of bamboo in phytoremediation. In addition, bamboo plants have good tolerance and uptake ability to heavy metals and have high application potential and development value in uptaking heavy metal contaminated soil. However, due to climate, temperature and other reasons, bamboo cannot be widely planted in most countries. Research status of remediation of heavy metal contaminated soil by bamboo plants is summarized. The feasibility of its application in heavy metal contaminated soil is discussed in this paper. Aiming at the shortcomings of existing research, bamboo plants have a prospect in the field of plant phytoremediation for the future.
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The effect of different herbicide on the number and activity of living microorganisms in soil
76-82Views:136Sustainable plant growth, considering the difficulties of weed elimination, cannot be effective without the application of herbicides. However, these chemicals have enormous ecological implications, including effects on the microbiological communities of soils. It is advisable to use herbicides that have minimal secondary effects on the environment and soil-living microorganisms. In contrast, herbicides with prolonged growth stimulating or inhibiting effects are not suitable, because both types have strong influences on the number and activity of bacteria, thus causing changes in the ecological equilibrium.
Preceding small plot experiments, laboratory tests were carried out to study the effect of herbicides used in maize cultures on the number of bacteria and growth of microscopic fungi.
Substances that were observed to have stronger influences were applied in small plot experiments set up in the experimental garden of the Department of Plant Protection of the University of Debrecen. We studied the effects of four herbicides (Acenit A88EC, Frontier 900 EC, Merlin SC and Wing EC) on the microbiological properties of the soil. These herbicides were used in different concentrations in maize culture, and we investigated the effects in different soil layers.
In the laboratory experiments, we determined the total number of bacteria and microscopic fungi and examined the growth of Aspergillus niger, Trichoderma sp. and Fusarium oxysporum on peptone-glucose agar containing herbicides.
During the small plot experiments, soil samples were collected 3 times a year from 2-20 cm depth. The total numbers of bacteria and microscopic fungi were determined by plate dilution method, while the method of most probable number (Pochon method) was used to determine the numbers of nitrifying bacteria and cellulose decomposing bacteria. To evaluate the microbiological activity of the soil samples we measured carbon-dioxide release (after 10 days incubation), nitrate production (after 14 days incubation) and the concentration of C and N in the biomass.
We can summarize our results as follows:
• In laboratory experiments, herbicides caused a decrease in the number of bacteria and inhibited the growth of microscopic fungi.
• Frontier 900 EC and Acenit A 880 EC had the strongest inhibiting effect on microorganisms.
• In small plot experiments, herbicide treatment decreased the total number of bacteria and microscopic fungi.
• Herbicides caused a significant increase in the number of nitrifying and cellulose decomposing bacteria.
• Different herbicides containing the same active compound had similar influences on soil microoorganisms.
• A significant increase was observed in the physiological processes of tolerant microorganisms surviving the effects of herbicides -
Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:323Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Assessment of Environmental Susceptibility/Vulnerability of Soils
62-74Views:100Soils represent a considerable part of the natural resources of Hungary. Consequently, rational land use and proper soil management – to guarantee normal soil functions – are important elements of sustainable (agricultural) development, having special importance both in the national economy and in environment protection.
The main soil functions in the biosphere are as follows: conditionally renewable natural resource; reactor, transformer and integrator of the combined influences of other natural resources (solar radiation, atmosphere, surface and subsurface waters, biological resources), place of „sphere-interactions”; medium for biomass production, primary food-source of the biosphere; storage of heat, water and plant nutrients; natural filter and detoxication system, which may prevent the deeper geological formations and the subsurface waters from various pollutants; high capacity buffer medium, which may prevent or moderate the unfavourable consequences of various environmental stresses; significant gene-reservoir, an important element of biodiversity.
Society utilizes these functions in different ways (rate, method, efficiency) throughout history, depending on the given natural conditions and socio-economic circumstances. In many cases the character of the particular functions was not properly taken into consideration during the utilization of soil resources, and the misguided management resulted in their over-exploitation, decreasing efficiency of one or more soil functions, and – over a certain limit – serious environmental deterioration.
Soil resources are threatened by the following environmental stresses:
– soil degradation processes;
– extreme moisture regime;
– nutrient stresses (deficiency or toxicity);
– environmental pollution.
Environmental stresses caused by natural factors or human activities represent an increasing ecological threat to the biosphere, as well as a socio-economic risk for sustainable development, including rational land use and soil management.
The stresses are caused by the integrated impacts of various soil properties, which are the results of soil processes (mass and energy regimes, abiotic and biotic transport and transformation and their interactions) under the combined influences of soil forming factors. Consequently, the control of soil processes is a great challenge and the main task of soil science and soil management in sustainable development.
The efficient control of these processes necessitates the following consecutive steps:
• registration of facts and consequences (information on land and soil characteristics, land use, cropping pattern, applied agrotechnics, yields, with their spatial and temporal variability);
• evaluation of potential reasons (definition and quantification of soil processes, analysis of influencing factors and their mechanisms);
• assessment of the theoretical, real, rational and economic possibilities for the control of soil processes (including their risk-assessment and impact analysis);
• elaboration of efficient technologies for the „best” control alternatives (best management practice).
Scientifically based planning and implementation of sustainable land use and rational soil management to ensure desirable soil functions, without any undesirable environmental side-effects, require adequate soil information. In the last years such data were organized into a computer-based GIS soil database in Hungary, giving opportunities for the quantification, analysis, modelling and forecasting of the studied environmental stresses and for the efficient and scientifically based prevention, elimination or reduction of environmental stresses and their unfavourable ecological and economical consequences.
Special attention was paid to the assessment of various soil degradation processes, as: (1) soil erosion by water or wind; (2) soil acidification; (3) salinization and/or alkalization; (4) physical degradation (structure destruction, compaction); (5) extreme moisture regime: drought sensitivity and waterlogging hazard; (6) biological degradation; (7) unfavourable changes in the plant nutrient regime; (8) decrease of natural buffering capacity, (9) soil (and water) pollution.
The actions against undesirable environmental stresses and their unfavourable consequences are important elements of sustainable, efficient, economically viable, socially acceptable and environmentally sound crop production and agricultural development. These are joint tasks of the state, decision makers on various levels, the land owners, the land users and – to a certain extent – of each member of the society. -
Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:91The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Magnesium uptake dynamism of maize (Zea mays L.) on prairie soil
83-89Views:86Different influence factors on the magnesium (Mg) uptake in case of three maize hybrids with different long vegetation period have been investigated at the Experimental Station of the University of Debrecen, Centre of Agricultural Sciences and Engineering, at Debrecen-Látókép. The soil of the experiment is a calcareous chernozem, based on loess, with high fertility, that is characteristic for soils of the region Hajdúság.
Upon irrigation is the experiment divided to main plots, by different hybrids into sub-plots, while treatments of five nutrientsupply levels with fixed N:P2O5:K2O rate (beside control)mean sub-subplots. Soil samples were taken from the upper, cultivated soil layer 3 times during the year 2008. Their pH has been measures in a 0.01 M CaCl2-solution and their Mg-content from the same solution and from ammonium-lactate acetic acid (AL) extract. Plant samples were taken seven times in the vegetation period, of which we measured the Mg-content. Beside this, the during the
vegetation period by maize biomass extracted Mg-amount has been calculated using fresh and dry matter weights. The effects of irrigation, hybrids and nutrient-supply levels on the soil pH and on the AL- and CaCl2-extractable Mg-amount have been studied, as well. After that I tried to find a correlation between soil pH and the Mg-content of soil determined in different extractants, beside this between the by the two solutions extracted Mg-amount. -
Estimation of the vine-shoot yield in the Gyöngyös district
21-26Views:136Every year, a significant amount of vineyard prunings is generated in Hungary. Instead of utilization them, it is burned directly on the field in spite of the limitations of legalization highly pollute the environment in recent decades. The vineyard pruning itself or with other by-products from pomology and forestry can be significant amount of fuel source. However, before the planning to utilization the essential task is to estimate the potential. This study examine that how many vineyard is generated in the micro-region of Gyöngyös in every year on appointed vineyard.
In order to vine-branch potential calculate more accurate data was necessary so the products had to be determined for all plant. Thus, the biomass resource and the utilization become plannable. As the result of the experiment we can estimate that 1,5 t/ha vineyard pruning are generated. It means more than 9 thousand tons pruning in the micro-region of Gyöngyös. This amount can be convert into fuel source more than 150 thousand GJ heating value.
The results evince that the vineyard pruning is important fuel source in every year in the micro-region of Gyöngyös. Further usage is suggested for other regions in other size.
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Investigation of the decomposition and leaching dynamics of Salix, Populus and mixed leaves in the area of Lake Balaton and Kis-Balaton Wetland
119-124Views:207In lakes and wetlands, leaf litter input from the coastal vegetation represents a major nutrient load and plays a basic structural and functional role in several ecosystems. In Hungary, at the banks of lakes and wetlands, Salix and Populus trees are the most common species. In an experiment in Lake Balaton and Kis-Balaton Wetland between 16 November 2017 to and 3 June 2018, the decomposition rates and leaching dynamics of Salix, Populus and mixed leaves (50% Salix and 50% Populus) were investigated. Total nitrogen and phosphorus content of biomass samples were measured at the beginning and end of the experiment for the leaching dynamics experiment. We found that litter mass losses (Salix, Populus and mixed leaves) were not significantly different between the two mesh size litterbags and between Lake Balaton and Kis-Balaton Wetland. Different amounts of the total nitrogen and phosphorus leaching from Salix, Populus and mixed leaves were detected. The total nitrogen contents of the plant samples were around 8-18% at the end of the investigated period. Slightly higher values were measured compared to phosphorous (27-29%).
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Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:91Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:157Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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Evaluation of the role of common vetch (Vicia sativa L.) green manure in crop rotations
161-171Views:206Common vetch (Vicia sativa L.) is an annual legume, grown as green manure provide rapid soil cover, can increase soil moisture and organic matter content and reduce soil erosion during fall. During the fallow period, legumes grown as catch crops are known by releasing large amounts of mineral nitrogen (N) for the subsequent crop. By taking advantage of these benefits, it is possible to increase the yield of the next crop in an environmentally friendly and sustainable way. The goal of this study was to determine the value of common vetch as a green manure, considering its effect on soil conditions and the yield of next crops. We examined three different common vetch seed rate as a green manure in a crop rotation with triticale, oat and corn. Next to the green manured treatments, we used fertilized and bare fallow control treatment for comparison. In our study we evaluated the aboveground biomass weight of spring vetch green manure and its effect on the moisture content of the soil. We examined the green manure’s effect on the next crops plant height and yield. We found that the moisture content of the green manured plots was significantly higher during summer drought. On the green manured plots, 37.9% higher triticale yield, 50% higher oat yield and 44% higher corn yield were measured compared to the control plots. The insertion of spring vetch green manuring into crop rotations could be a good alternative to sustainable nutrient replenishment methods. It can be used to reduce the input needs of farming, reduce carbon footprint, contribute to the protection of soils and increase the organic matter content of the soil.
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The Presentation of Debrecen University’s Solar Energy Power Plant
259-263Views:97Energy plays an important role in everyday life and in the economy. The use of fossil energy sources greatly damages the environment. Besides this, the quantity of these energy sources is limited. Therefore, it is important to increase the share of renewable energy sources (solar, wind, water, biomass) in energy generation. Huge amounts of energy (1150-1300 kWh/m2) arrive at the earth from the sun, which utilized in passive and active ways. One of the active applications is photovoltaic current production, in the course of which electricity is produced directly with PV-Panels. This can be fed into the grid.
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Leaf protein analyses in order to utilise the leaf shoot of artichoke
43-47Views:163The constat growth of the Earth’s population brings with itself a higher demand for food and protein not only in human nutrition but also for the feeding of livestock. Currently, the feed industry is mainly built onseed-based protein, wherethebaseplant is soybean, which is large lycovered by imports in Hungary, similar toother European countries. However, the long-term economically sustainable lifestock breeding demandschanges which has also worked out strategies. An alternative protein sources could be green leafy plants.
In current work the Jerusalem artichokes as an alternative source of protein was studied, compared to alfalfa as a valuable protein plant. Our results indicate that fiber fraction ofJerusalem artichoke shootswas 34 to 37% after pressing in the autumn period while alfalfa slightly lower values were obtained (30%). On the other hand extracted green leaf protein concentration was higher in alfalfa than in Jerusalem artichoke. Along with this higher protein content could be measured from the leaf protein concentration of alfalfa and almost each amino acids were more, as well comparing to Jerusalem artichoke.
Overall, the alfalfa proved to be advantageous as expected both in leaf protein extraction efficiency, both regarding the content of the protein in the Jerusalem artichoke. However, considering aminoacid composition and green biomass production, Jerusalem artichokecould be a promisingplant species asplant protein sourceinthefuture.