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Nutritional disorders of arable crop growth in eastern Croatia
273-290Views:136Nutritional imbalances accompanied with growth retardation of crops at early growth stage were found since the last 40 years on certain arable lands in eastern Croatia. In this regard, phosphorus (P) deficiencies in maize and wheat were found mainly on acid soils of the western part of the region, potassium (K) deficiencies in maize, soybean on the hydromorphic neutral to alkaline calcaric drained gleysols of Sava valley lowland, while zinc (Zn) deficiencies were observed mainly in seed-maize and soybean on neutral calcaric eutric cambisols of the eastern part of the region. Cold and moist spring is factor promoting P deficiency symptoms. As oasis of normal crops existed on same arable land, comparison of plant and soil composition was possible from typical sites. P nutrition disorders were in connection with the lower P and the higher aluminum (Al) and iron (Fe) concentrations in the top of plants and the lower soil pH values. K-deficiency as result of strong K fixation and imbalances with high levels of magnesium (Mg) were the main responsible factors of low maize and soybean yields on some drained gleysols. Chlorosis incidences typical for Zn deficiency in maize and soybean were in close connection with the higher soil pH, the lower quantities of mobile Zn, here and there the higher mobile P in soil, the lower concentrations of Zn and the higher levels of Al and Fe in plants. Overcoming the above mentioned disorders and normalization of yields were achieved using ameliorative fertilization either by K or P fertilizers and in case of Zn by foliar spraying of crops with 0.75% ZnSO4 solution. Also, alleviations are possible by selection of more tolerant genotypes of field crops to specific types of nutritional disorders. From this aspect, some practical solutions were recommended for maize with reference to K nutritional problems.
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Analysis of the photosynthetic parameters, the yield and the quality of winter wheat
101-106Views:125The environmental adaptability of crop production is basically determined by the selection of biological background (plant species and
varieties) suitable for the region and the site. The aim of our work is to parametrize the plant assimilation, its intensity, dynamics and the
most important characteristics and the relationships to the quality in winter wheat trials. The measurements were carried out at the research
site of the University of Debrecen in small parcel experiments. We measured the leaf net CO2 assimilation rate, stomatal conductance,
intercellular CO2 level, the transpiration, the leaf temperature and the air temperature by the LICOR LI-6400 portable photosynthesis
system in field trials on the nutrient supply. The soil of the experimental area is calciferous chernozem with favorable water regime.
We have examined the photosynthetic activity, the productivity and yield stability of winter wheat varieties. We have compared the yield
results, at similar agrotechnical conditions in seven cropyears. We also determined the quality parameters of the winter wheat varieties.
Then we valued the yield stability of genotypes with the help of analysis of variance and linear regression equations. We have defined the
connections between assimilation parameters, the yield stability and quality parameters of wheat varieties. -
Evaluation of blue and red corn landraces
239-243Views:73Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
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The imact of crop rotation and fertilisation on the SPAD values of winter wheat on chernozem soil in a long-term experiment
123-126Views:126We have carried out our outdoor field experiments at the Látókép Experimental Farm of the CAS of the University of Debrecen in the cropyear of 2012/2013 on chernozem soil in a long-term experiment. We have studied the effects of two different preceding crops (sweet corn, sunflower) on the development of the SPAD values of wheat varieties of different genotypes in the cases of control, N60+PK and N120+PK fertilizer treatments. According to our research results, we have concluded that the preceding crop, the fertilizer application and the variety selection influenced the SPAD values. According to our data, we have measured higher SPAD values after sweet corn preceding crop, the standard deviations were in a smaller range in the case of the studied varieties. After sunflower preceding crop, smaller averages were experienced with wider standard deviation range. In the case of favourable preceding crop, the differences between the varieties are more pronounced than in the case of unfavourable preceding crop. The maximal SPAD values were measured in milky ripening in the case of N120+PK fertilizer treatment.
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Timing it right: The Measurement and Prediction of Flowering
17-22Views:60Although the model described here was developed from research in controlled environments, there is now considerable evidence that in can be applied to a very wide range of natural environments in several species. Multi-locational trials augmented by successional sowing and, if considered necessary, supplementary illumination in the field to increase daylength, can be used to estimate the values of the model coefficients: (1) to characterize germplasm collections and so predict flowering behaviour elsewhere; (2) for interpreting and understanding crop adaptation; and (3) for genetic analysis of photoperiod sensitivity. We do not yet know whether the model has any contribution to make to the understanding of the biochemical mechanisms of photoperiod and temperature responses, but at the very least, it should provide the basis for indicating the most appropriate environmental conditions, genotypes and physiological stage of the plants most suitable for such investigations.
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Investigation of the heat shock gene Hsp70 polymorphism in different sheep genotypes
41-45Views:126Nowadays the climate change has an increasing effect on the animals. The warming climate brings up several problems on the area of the animal husbandry, which ones are really important. From the first time the living beings have defensive mechanisms against the heat shock. In current examination we use– from Hungary and from other countries collected –samples of sheep breeds, which are living on different climate. Our fundamental assumption was, that the animals living on other climate adapted to the changes of the environment and there are differeces in their genetic background. These fixed mutations we are looking for in the HSP70 heat shock gene, but we haven’t found any polymorphism yet. We are going to involve further breeds and more individuals in the investigations.
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Genetic structure of the Lipizzan horse breed in Hungary through the mare families
71-78Views:95Modern animal husbandry has drastically changed the genetic structure of some domestic species. The varieties, genotypes that we think we do not need at the moment can only be saved from extinction with the help of gene conservation. Traditional Hungarian horse breeds have a long history (more than 200 years) and a demonstrably different genetic structure from other horse breeds in Europe and the rest of the world. Consequently, their enormous genetic value is undoubted. The subject of our research was to study the structure of the mare families found in the population of the Lipizzan horse breed and the genetic structure of the Hungarian population. Out of the total 61 mare families in the world, 35 are present in Hungary. There are 11 Hungarian, 12 families of Fogaras, 7 original, 4 Croatian and 1 Slovenian mare families in the Hungarian population. The proportion of mare families in Hungary and Fogaras is almost the same. The proportion of Croatian and Slovenian families is negligible. In terms of their number, they are not significant in Hungary. For this reason they have little effect on the Hungarian Lipizzan population. Except one of the original mare families are in the same situation as the Croatian and Slovenian families. The Presciana / Bradamanta mare family is the most populous of all families due to their long stay in Hungary. The proportion of families is unbalanced. Seven mare families accounting for 56.36% of the total population. Given the basic requirements of gene conservation work, this condition is far from optimal.
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Examination of Fattening and Slaughtering Performance of Hungarian Merino, Ile de France F1 and Suffolk F1 Lambs
16-23Views:67In our examination, we evaluated Hungarian Merino (ram n=30, ewe n=30), Ile de France F1 (ram n=10, ewe n=10) and Suffolk F1 (ram n=10, ewe n=10) lambs. The best fattening performance was shown by the Ile de France F1 lambs (361 g/day). After the fattening period, Hungarian Merino, Ile de France F1 and Suffolk F1 genotypes lambs were slaughtered with a live weight of 30-34 kg. Carcasses were evaluated for dressing percentage, weight of valuable carcass cuts, percentage of valuable meat, bone to meat ratio, as well as meat conformation and fat cover (S/EUROP grading).
The slaughter performance proved to be the best for the Suffolk F1 concerned dressing percentage (51,7%), percentage valuable carcass cuts (81,9%). The best percentage valuable meat presented (79,4%) the Ile de France F1 lambs. The best meat conformation and fat covered were in the Suffolk F1 lambs; Hungarian Merinos showed less favourable results.
Presently, the results derived from Hungarian practice that Hungarian Merino lambs are not eligible for fattening to great live body weight (30 kg). Therefore, it is necessary to revise the Hungarian Merino lambs with meat sheep breeds. -
Folic acid content of beetroot leaf and root by different growing stages and genotypes
115-119Views:219An increasing interest has been observed of beetroot leaf as a salad component due to recent studies focusing on their nutritional value. The randomized field experiment was carried out on lowland chernozem soil with 6 varieties, 3 replications and 2 sowing dates. Sampling was performed on 23 of August 2018 at the stage of 30 and 50 days of vegetation, where leaf (30 and 50 days) and root (50 days) were collected. Total dry matter, folic acid and nitrate content were evaluated.
The results of this investigation show that higher total dry matter content was measured in the root (8.47–10.30%) compared to the leaf in both developmental stages (6.47–9.20%). Nevertheless, higher folic acid content was found in the young leaves of 30 and 50 days of development (58.77–113.86 µg 100g-1). Among the examined varieties, Bonel has presented great amount of folic acid not only in the leaves (99.35–113.61 µg 100g-1), but also in the root (89.99 µg 100g-1). Finally, lower nitrate content was found in Libero (316.16 mg kg-1) at 30 days and in Akela (340.41 mg kg-1) at 50 days of development. Thereby, fresh consumption of beetroot leaves are highly recommended.
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Evaluation of decreasing moisture content of different maize genotypes
147-151Views:225An experiment was conducted to evaluate the decrease in grain moisture content in three maize hybrids in Debrecen in 2017. Armagnac, Sushi and Loupiac were the examined hybrids in this study. The culture medium and temperature conditions were applied uniformly for all three hybrids. According to the results obtained from the ratio of moisture content of seeds per day, the Armagnac variety in the intensive drying down phase loses more time and moisture content, so it can be concluded that the produced dry matter is more than in the case of other varieties. Armagnac requires more time to achieve yield, while the Sushi and Loupiac hybrids produce less harvest. Regarding the slope of the regression line, the rate of loss of moisture in the grain has been negatively correlated with the amount of “b” in the three examined hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97% in the Loupiac, 95% and in the Sushi 90% of the total dynamic value of moisture motion.
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Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen
133-138Views:237The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in undeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.
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Evaluation of reaction to plant density of sunflower hybrids by regression and Kang’s stability analysis
101-109Views:91The world production area and the total production of sunflower has significantly been growing. The harvested yield was 23.4 and 21.1 million ha in 2005 and 2001, respectively. The total sunflower seed production has also unexpectedly increased.
Although sunflower is produced on lower quality soils in Hungary, in 2005 the average harvested yield was 2.43 t ha-1, which was the highest in the world.
Sunflower is a typical commercial plant and fits well in the crop structure. Since in terms of acreage the most significant crops are corn and cereals, the partial monoculture cultivation cannot be avoided. Sunflower production is a way to eliminate this problem, therefore it has an important role both in cultivation and ecological points of view.
Accordingly, sunflower has an important role in reducing the monoculture cultivation of some plants, as well as increasing biodiversity. Sunflower well adapts to Hungary’s climatic conditions and its production is easily practicable in our country.
The reaction of sunflower hybrids on crop density change is different. Some hybrids are more some are less sensitive to this parameter. In different crop years, the crop density optimums of the different genotypes are also different. In Hungary, the yield and quality is primarily determined by fungal infections, while viruses and bacteria are less important.
The research was conducted at the Látókép farm and Regional Research Institute of the University of Debrecen, Centre of Agricultural Sciences. The research institute is situated by Road 33,15 km from Debrecen in the Hajdúság. The duration of the experiment was seven years, 10 hybrids were examined in each year.
Two hybrids used every year, Aréna/PR and Alexandra/PR hybrids were tested by Kang’s stability analysis. We found that Alexandra/PR was most balanced at every levels of crop density. Both hybrids performed most stable yield at 65000 ha-1 crop density level and less balanced at 35000 ha-1 crop density level. As a result of improved environmental conditions, the yield increase of Aréna/PR was higher than that of Alexandra/PR.
Our regression analysis found that the maximum yield of Lympil, Louidor, Hysun 321, PR63A82 and PR64A63 hybrids were harvested at 47000-60000 plant ha-1 crop density level. The statistical analysis showed that the highest yield was harvested from Lympil and Hysun 321. As regards the crop yield, the most stable hybrids were Louidor and Lympil. The optimum crop density interval of Rigasol/PR and Larisol (58000 plant ha-1) was wider than that of Diabolo (46000 plant ha-1). The maximum yield of Larisol was higher at the optimal crop density level. As regards yield, Diabol was the most stable hybrid.
The statistical analysis on the stability of the yield of Alexandra/PR and Aréna/PR showed that Aréna/PR is more stable, and its optimal crop density level is lower than that of Alexandra/PR. -
Age of puberty and conception rates of Hungarian Merino, (Merino x Somali) F1 and (Merino x Barbados Blackbelly) F1 ewes
15-19Views:163The aim of our examination was to detect the puberty of the Hungarian Merino and its hairsheep crossbred ewes, and also to determine their average conception rates after having ram exposed in May and June. Hormon diagnostation was used to evaluate the exact date of the very first ovulation. Ultrasound technique was used for checking the conception rates of ewes. The pregnancy detection was applied after a month of the date of taking off the ram.
We evaluated the ages of ewes at puberty. Statistical deviation and standard error were calculated. The results proved the Hungarian Merino x Somali (N=10), ((x ± s) = 173 ± 43) and also the Hungarian Merino x Barbados Blackbelly crossbred genotypes (N=7), ((x ± s) = 186 ± 19) have their puberty much earlier as compared to the fullbred Hungarian Merino ewes (N=10), ((x ± s) = 231 ± 95). We experienced the highest conception rate in the group of the (Hungarian Merino x Somali) F1 ewes after exposing the ram in the „out-of season” period of time. -
Effect of genotype on the hematological parameter of TETRA-SL and Hungarian Partridge coloured chickens at young age
99-104Views:181Indigenous chickens are well known for their dual-purpose function and palatable meat. However, the information on their hematology parameters is lacking and hence hampering the poultry industry production of local breeds. The main objective of this study was to examine the hematological parameters of Hungarian Partridge Coloured hen (HPC) and TETRA-SL (TSL) genotype and determine the hematological reference interval values. This trial was part of the larger experiment where a total of 200 chicks (HPC + TSL) were reared. For this trial the blood samples were obtained from brachial wing veins of 8 chicks of each genotype for blood hematological analysis. The results indicated that some of the hematological parameters such as red blood cells-RBC, white blood cells-WBC, hemoglobin-Hb, hematocrit-Ht, platelets-Plt, lymphocytes-LYM, and granulocytes-GRAN were significantly affected by the genotypes (p < 0.05). The genotype did not affect the mean corpuscular volume-MCV, mean corpuscular hemoglobin-MCH, mean corpuscular hemoglobin concentration-MCHC, and GRAN (p > 0.05). The hematological reference interval values were slightly higher in the TSL genotype compared to HPC chicks. It is concluded that genotype has a significant effect on the hematological parameters. The results from this trial will be help and design the baseline reference values for the HPC genotype which will be useful in assessing the health status of these indigenous chickens.
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Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:88The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Comparison of the physiological responses of an oil-pumpkin cultivar and hybrid under different nitrogen supply conditions
65-69Views:177Cucurbita pepo var. styriaca is known as a medicinal crop among other cucurbits. The benefits of cucurbits fruits are very important in terms of human health, purification of blood, removal of constipation, digestion and supplying energy. The mutant styrian oil-pumpkin (Cucurbita pepo L. convar. Pepo var. styriaca Greb.) exposes a complete lack of lignification of the seed testa. For this reason, this kind of naked pumpkin seed is more consumable as snack than other seeds. The vegetative growth of plants is crucial for fruit production. Nitrogen is the most imperative element for the proper vegetative growth and development of plants which significantly increases and enhances yield and its quality by playing a vital role in the biochemical and physiological functions of plants.
Field studies were conducted to study the effect of nitrogen nutrition on certain physiological parameters and their differences between the styrian oil pumpkin hybrid and cultivar (Gleisdorfi Classic cultivar and GL Rustical hybrid). The small block experiment was carried out based on a factorial experiment with a completely randomised block design and four replications in the Research Institute of Nyíregyháza during the summer period of 2017 and 2018. Three different concentrations of nitrogen fertiliser was applied at sowing. Photosynthetic pigments (chlorophyll-a, -b and carotenoids) were analysed as one of the basic factor of efficient photosynthesis. The yields of two genotypes were also measured in all treatments as seed-weight. The contents of photosynthetic pigments were higher in the oil-pumpkin hybrid, mainly the carotenoids in 2018. The vegetation period of 2018 was drier than in 2017, the hybrid oil-pupmkin was more efficient in stress tolerance than the cultivar with higher carotenoids pool. The obtained yield was more pronounced in the oil-pumpkin hybrid than the cultivar in all examined years. The hybrid GL Rustical was more sensitive to nitrogen treatment than the cultivar Gleisdorfi Classic.
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Test of the Plant Density Reaction of Genotype Sunflower Hybrids
113-119Views:111In order to produce sunflower in Hungary today it is important to develop hybrid-specific cropping technologies. The ever widening number of hybrids makes the constant examination of genotypes necessary from the viewpoint of genotype-environment interactions and critical elements. Plant density as a complex factor puts strain on the pathological features, yield and quality of sunflower. The experiment’s main objective is to find the optimal plant density for both the genotype and external factors.
As a result it can be stated that the optimal crop density is between 45,000-75,000 plant/ha. In 2001 the optimal density was 55,000 plant/ha. The Aréna PR and the Alexandra PR hybrids produced the greatest yields (3511 kgha-1; 3338 kgha-1). In the growing season of 2002, the yields were higher than in the previous year and the optimal crop density was 45,000-65,000 plant/ha. The best yields were produced by the Aréna PR and Alexandra PR hybrids in this year again (4102 kgha-1; 4267 kgha-1) and in 2003, 45,000-65,000 plant/ha proved to be the best crop density. The highest yield was produced by the Alexandra PR.
Analyzing the growing seasons of 2001, 2002 and 2003 it can be declared that as a result of dry climate of the three years yields were higher. It can be stated that the yield is decreased by higher than average of precipitation in the growing season. -
Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104Views:118The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations. -
Improvement of black locust (Robinia pseudoacacia L.) growing under marginal site conditions in Hungary: case studies
129-133Views:317In Hungary, black locust (Robinia pseudoacacia L.) is considered as an important exotic stand-forming tree species growing mostly under unfavourable ecological conditions for forest management. Due to climate change effects, its importance is increasing in many other countries, too. As a result of a selection programme, new black locust clones were tested in clone trials. Juvenile growth of 12 micropropagated black locust clones in two plots series established at different dates were evaluated in central Hungary under marginal site conditions. At age of 7 the clone R. p. ‘Bácska’ (‘KH 56A2/5’), at age of 10 the clones R.p. ‘Homoki’ (‘MB17D3/4’) and ‘PV201E2/4’ appeared to be especially promising for mass production. Based on the data obtained from the performed trials, it can also be concluded that tissue culture can be considered as a suitable tool for propagating superior individuals and offers new prospects for the rapid cloning of selected genotypes used for plantation forestry.
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Effect of the fertilization on some quality parameters of winter wheat
105-110Views:89We studied the different mineral fertilizer rates effect on the baking quality of five different winter wheat genotypes (GK Öthalom, Lupus, KG Széphalom, GK Békés, Mv Mazurka) on chernozom soil in 2008.
The bigger fertilizer doses increased the wet gluten content, gluten elasticity and the protein content. The high doses of fertilization has got a moderate effect on the valorigaph value, water absorption, and falling number. The optimum level of the fertilizer doses were the N90-120+PK.
The Pearson’s correlation analysis proved strong relationships between the fertilization and gluten content, the quality of the gluten, a valorigraph value, and the protein content. The analysis verified a strong correlation between genotype and falling number, and the protein content.
The variety Mv Mazurka has got the best baking quality parameters. -
Correlation between cultivation methods and quality in some vegetable species
313-317Views:111Quality parameters of 5 table root varieties were tested on 3 sowing dates with different cultivation methods: open field on 15 April and 9 July 2010 and under plastic tents on 19 August. The highest red pigment content (betanin) was measured in the varieties Akela and Mona Lisa (~ 80 mg 100 g-1) of the second (July) crop. This crop is in general use in Hungary. In comparison, in the late sown varieties (August, under plastics) a further pigment increase (10–20 mg 100 g-1) was observed in the same varieties as related to the earlier sowing dates. Yellow pigments (vulgaxanthins) showed similar trends. Roots of the late sowing date (with harvest in December) contained the highest vulgaxanthin values (103.3–124.18 mg kg-1).
Varieties reacted differently to temperature changes during the production period and thus to sugar accumulation. In the second crop (July) higher water soluble solids content was measured on the average of varieties (10.12%) in comparison to the April sowing (7.76%). Beetroots of the spring sowing are recommended for fresh market while the second (July) crop with autumn harvest can satisfy industry requirements. Late sowing under unheated plastic tents supply us with fresh beetroot in late autumn and early winter and prolong the usability of plastic tents.
Six lettuce species/subspecies were tested in the open field and under plastic tents in 3 repetitions for nitrate nitrogen, vitamin-C, polyphenol (gallus acid equivalent – mg GAE 100 g-1) and mineral element (Ca, K, Mg, Na) contents. Our measurements showed lower nitrate nitrogen values under plastic than in the open field (89.10± 8.13 and 127.06±14.29 mg kg-1) on the average of genotypes. Lettuce grown in the field had higher vitamin-C content (1.4 mg%) which is nearly 50% more than in plants under plastic. The highest polyphenol content was found in samples from the field with a conspicuous value of 804.17±56.47 mg GAE 100 g-1 in Piros cikória. Samples grown under plastic were richer in mineral elements (Ca, K, Mg, Na) which can be explained by the higher nutrient content of the soil. In this environment superior Mg content was observed in Edivia (4616.33±
311.21 mg kg-1).Besides the well- known headed lettuce, Piros cikória (Red chicory),the red leaved Lollo Rossa and Tölgylevel (Oak leaf lettuce) should be
mentioned which well deserve further testing in order to supply us with nourishing, healthy food. -
Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:83We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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Effect of the plant density on different maize (Zea mays L.) hybrids yields and leaf area index (LAI) values
51-56Views:145We have investigated the plant number reactions of three maize hybrids of various genotypes in a small-plot field experiment. The plant numbers were 50, 70 and 90 thousand ha-1, while the row distances were 45 and 76 cm. The experiment was set on the Látókép Experimental Farm of Centre for Agricultural Sciences of the University of Debrecen in four replications on calcareous chernozem soil.
The assimilation area and the leaf area index have important role in development of the crop yield. The studied three different genotype maize hybrids reached its maximum leaf area index at flowering. The maximum leaf area index increased linearly with increasing plant density. The season-hybrids reached less yield and leaf area index. According to our experimental results, we have concluded that with the decrease of the row spacing, the yield increased in the average of the hybrids. The studied hybrids reached the maximum yield at 70 and 90 plants ha-1 plant density. We determined the optimal plant number that is the most favourable for the certain hybrid under the given conditions.The higher plant density was favourable at 45 cm row spacing than 76 cm. The hybrids reached the maximum grain yield at 45 cm row spacing between 76 712–84 938 plants ha-1, while the optimum plant density at 76 cm row spacing changed between 61 875–65 876 plants ha-1.
The leaf area index values between the applied plant density for the flowering period (July 1, 24), we defined a significant differences. In the archived yields were significant differences at the 45 cm row spacing between 50 and 70, 90 thousand ha-1 plant density, while the number for the 76 cm row spacing used did not cause a significant differences in the yield. There were significant differences between the examined hybrids of yields.