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The role of some elicitors in inducing chilling stress resistance in apricot fruit
27-32Views:163This study aimed to study the role of 2 mM salicylic acid and 0.2 mM methyl Jasmonic acid in reducing the chilling injuries on two different apricot varieties fruits (Bergarauge and Flavor cot). These treatments were applied for apricot fruit directly after harvest after measuring the zero time data. Then the fruit were stored at 1 oC for three weeks and were examined 7 day periodically, other group of fruit was stored at 1 oC for 15 days then transport to room temperature and examined after 4 and 8 days. The fruit samples were collected at each examination time, and the measurements containing the fruit firmness (Kg cm-2), chilling and decay index, membrane electrolyte leakage, total phenol content and phenylalanine ammonia lyase mechanism (PAL) activity. The results showed that there are no obvious differences between the tested varieties and the differences may back to genetic variations and they took the same trend for most of measurement after treated with SA and MJ. SA and/or MJ treated fruit achieved low percentage of fruit softening in comparison to water treated fruit. At the same time the chilling and decay index showed that the SA and/or MJ reduced the decay or breakdown symptoms in either tested varieties. The treated fruit with those elicitor help the fruit to keep about stable phenol content while water treated fruit lose the phenol content sharply at early storage and this stable level of phenol my because of the ability of those chemical to raise PAL activity in treated fruit.
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Differences in content of beet root and beet root juice, and it’s suitable to develop functional foods from animal sources
53-57Views:353In Western medicine is beginning to increasingly focus on the key role of healthy nutrition and lifestyle in preventing certain diseases. Therefore, it is important to emphasize the importance of primary prevention in our country, and to promote it; not only the health care team work, but broaden the recommended foods, which have benefit for human health as well.
Polyphenols are antioxidants, which are easily consumable with diet, however, food processing can influence the level of them.
In our study, nutritional assessment was made of raw beet root and a juice, made from this beet root. We investigated in which special diet referenced to eat this foods and whether this is suitable to develop functional foods from animal sources as a row material.
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Adopt the tobacco plastid transformation technics in Hungary
73-76Views:95Under the GENOMNANOTECH Debrecen Regionális Egyetemi Tudásközpont (GND RET) research program in summer 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture experiments were launched to transform tobacco plant by plastid transformation technics.
Our aim was to adopt first in Hungary the tobacco plastid transformation technics, which were used in Waksman Istitute Rutgers, The state University of New Jersey (USA) leading with Prof Dr. Pál Maliga. Scientists won scholarships learn and use this technics in the University of Debrecen. -
Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:91The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Application of microsatellite fingerprints for pedigree analysis of Hungaricum grapevine varieties
71-77Views:151the Carpathian Basin were involved into our examination, which aimed at genotyping their accessions. DNA fingerprints of 101 varieties were determined with 6 microsatellite markers till 2005, resulting in successful discrimination of the accessions. Based on these results for pedigree determination, even more cultivars and primers were involved into the analyses. For studying the origin of Csabagyöngye and for proving the parent-progeny relations of Irsai Olivér and Mátrai muskotály, 19 microsatellite markers were applied, while 11 were selected for tracing the origin of Királyleányka. Genetic distances between the varieties were estimated with cluster analysis and demonstrated by dendrogram, proving that the varieties can be discriminated from each other based on the microsatellite allele sizes. Pedigree of Irsai Olivér and Mátrai muskotály has been confirmed by microsatellite allele size results, searching for the parents of Csabagyöngye and Királyleányka is in progress, since the molecular-marker based pedigree does not correspond with the putative origin of these cultivars. Our results excluded progeny-parent relationships in the
Csabagyöngye-Bronnerstraube-Muscat ottonel (Ottonel muskotály) and the Királyleányka-Kövérszőlő combinations. -
Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:114The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Fruit melanotic ringspot (FMRS) – a disease of resistant Capsicum genotypes infected with Tomato spotted wilt virus (TSWV) on the fruits
64-69Views:89Etiology of pepper fruit melanotic ringspot (FMRS) disease (Salamon, 2009) was studied on fruit samples collected in forced pepper populations. It was noticed that in spite of heavy thrips (Frankliniella occidentalis) infestations and of TSWV epidemy detected in the forcing houses, FMRS occurred only in plants having healthy foliage. Symptomatological surveys strongly suggested that FMRS appeared exclusively in specific pepper genotypes. The size of melanotic ringspots has been observed to grow at room temperature during postripening of diseased fruits. A mechanically transmitted plant virus was isolated from symptomatic parts of 9 white pepper fruits affected by FMRS. On test plants each of the virus isolates caused systemic symptoms characteristic to TSWV. Using cDNA/PCR technique and TSWV N-gene specific primers a ca. 300 bp long DNA fragment has been amplified from total nucleic acid extracted from symptomatic tissues but never from asymptomatic parts of the fruits showing FMRS. Plant progenies grown from seeds of FMRS diseased fruits segregated in respect of resistance and/or susceptibility to TSWV infection. TSWV was also detected in and isolated from three fruits showed non-melanotic yellow rings (one of them was infected with a tobamovirus, too). Seedlings derived from these fruits proved to be susceptible to TSWV. Based on the above results we could conclude that the FMRS disease developed on fruits of “cecei” type white peppers that carry a TSWV resistance gene, most likely the Tsw gene in heterozygous form. These fruits were infected with thrips transmitted TSWV and FRMS appeared as a hypersentive reaction (HR) manifested in fruits.
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The Fattening and Slaughtering Examination at the Hungarian Racka Sheep
37-42Views:108Fattening and slaughtering characteristics of both types (black and white) of the Hungarian racka sheep were investigated at the site of the National Institute for Agricultural Quality Control in Atkár. The Hungarian merino, the dairy cigaja and the British milksheep were applied as control groups. 10 animals were examined from both sexes in each genotype. The examination was carried out according to the principles set in the Codex for sheep breeding. During the test acute diarrhoea occurred in each control group. However, only few lambs were excluded from the test from the British milksheep and the Hungarian merino groups because of significant changes in their condition. Consequently, the results obtained were not significantly influenced. No symptoms of any illness could be recognised in the Hungarian racka sheep, though. The significance of the difference between the average of the examined groups were checked by T-tests.
Daily gain was significantly less compared to the control groups, except for the white racka males. The lambs starving for 24 hours before slaughtering weighed 25.3-30.0 kgs on average. The black and white racka females weighed the least while the Hungarian merino females weighed the most. The quantity of abdominal and kidney fat was significant in the case of our native females. The quantity of abdominal fat was significantly bigger compared to each control group. On comparing the investigated groups it turned out that the white racka males had the biggest skin weight while the white Hungarian racka females had the smallest, that is the two extremes were recognised in the same colour (white) of one bred. On qualifying the slaughtered animals females had better results than males in each bred; the racka sheep were better than the dairy cigaja but were worse than the Hungarian merino and British sheep groups.
The huge differences in the carcasses of the groups were seen clearly when chopping. Examining the leg, it was noticed that the native groups proved to be smaller than the control groups, except for the black racka males. As for the short loin, the Hungarian racka and the Hungarian merino sheep proved to be the best, whereas the dairy cigaja and the British milk males had much smaller ones. The results gained when investigating the rack were similar to those of the short loin. As far as the rib and shoulder are concerned, the black racka females and the dairy cigaja females had the worst results. After chopping, the right side was also boned in every group. The small proportion of bone in the case of racka sheep was easily noticeable that resulted in the fact that the white racka females had the highest quantity of short loin compared to the other groups. When examining the back quarter, the bone proportions of the 4 racka groups proved to be significantly less than those of the control groups but it was not true for the meat proportions. As for the front quarter meat, the racka males were prominent in the great shoulder meat and in the small proportion of bone. The quantity of the first quarter bone was the least in the case of racka sheep and was the biggest for dairy cigajas. This difference proved to be significant in most cases.
In short, the Hungarian racka groups had the best results in meat quantity, which was especially significant compared to the dairy cigaja sheep. However, the quantity of external fat increased. -
Results of fast neutron induced mutations in M1 and M2 generations of sorghum (Sorghum bicolour)
49-54Views:98Due to climatic barriers, the genetic variability of Sorghum varieties decreases away from the gene centre, therefore hybrids cultivated in Hungary are restricted to a relatively limited genetic basis. This limited genetic basis results in a lower rate of genetic improvement, so the genetic basis of cytoplasm factors are to be extended. We enhanced the diversity of primary materials by fast neutron treatment. As a result of this treatment we detected sterile, deformed specimens with double panicles in the stock treated with a dose of 12.5 Gy in the M1 generation. Agronomically useful
specimens were found in the stock treated with 10 Gy and 7.5 Gy doses, where the leaf area index (LAI) and Harvest- index gave higher values than those of the control group. In the stock treated with 5 Gy dose, variations were less significant. In the reseeded M2 generation we detected albino specimens with extreme chlorophyll defects in the 10 Gy Zádor stock. -
Investigation of genetic diversity in irradiated maize lines and its relation to hybrid performance
20-26Views:228Knowledge of genetic diversity among available parental lines is fundamental for successful hybrid maize breeding. The aims of this study were to estimate (1) genetic similarity (GS) and genetic distance (GD) (based on Jaccard index) in four maize inbreed lines; (2) to classify the lines according to their GD and GS; (3) to determine hybrid performance based on GD and heterosis for yield ability in 4x4 full diallel system. We used morphological description and AFLP (amplified fragment length polymorphisms) for estimation genetic polymorphism in four maize inbred lines. We estimated the applicability of genetic similarity in SC and reciproc hybrids for prediction of their performance.
Three primer combinations were used to obtain AFLP markers, producing 207 bands, 70 of whit were polimorphic. The dendogram based on genetic similarities (GS) and genetic distance (GD) and morphological description separated four inbred lines into well-defined groups. Morphological description just with AFLP analysis showed reliable results. In view of genetic distance, the UDL 1 line and their linear and reciprocal crosses showed significant heterosis effect, which was confirmed by heterosis calculation based on grain yield. -
Alternatives of the multiple use of Virginia mallow (Sida hermaphrodita L. Rusby) as a shrub-replacing mallow in the temperate climate zone
51-56Views:172In the seventies of the previous century, Dr. Zoltán Kováts set two directions in the research of mallows. One of the directions was the biotechnology of the mallow species and the other direction is using the mallow species as biomass material. In order to do this he brought mallow mother spawns of ornamental and biomass sorts from botanical gardens abroad and tested many of them, including the a Sida hermaphrodita kind. Fourty years later, for the second time this plant, known as the „energy mallow” got back to Hungary again, right into the sight of hungarian biomass business with the help of László Balogh and his associates using help from Poland. This genus got into the center of our research, because of it’s valuable attributes. The latest experiments are about using it as an energy plant, without examining genetic details. The plant grows up to more
than 3 meters, has high growing rate and produces big amount of green mass. We don’t have any hungarian data about whether the plant continues the sufficient growing rate or not, after cutting it back.
We have to explore the potentials in the Sida’s sublimation. The plant is mostly suitable for ornamental and energy utilization, so further sublimation should be aiming for these aspects. During my research period, we are willing to get to know these potentials and the best possible use of them. -
Lambing Frequency in the Ile de France, Suffolk and Babolna TETRA Stocks of Babolna c.o., Szendrő
29-32Views:179Lambing frequency of three different genotypes was evaluated in the breeding stock of Babolna c.o. Szendrő. Ile de France, Suffolk and Babolna TETRA breeding ewes gave the data for the further evaluation. The main aim of this research was to find out if there is any significant effect of selected parameters (registration class, lambing season, lambing type and the years) on the lambing frequency, the period between consecutive lambings. Production parameters of 9,471 Ile de France, 1,244 Suffolk and 7,618 Babolna TETRA ewes were collected and processed. The analysis covered the period between the years 1989-1999. It was concluded that not all of the evaluated parameters had a significant effect on lambing frequency. Not all of the genotypes showed significant differences, according to the examined parameters.
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Determining factors of test weight in maize (Zea mays L.)
40-42Views:147Most domestic maize production products are sold on markets abroad. Among the increasingly restrictive quality requirements, the demand for the measurement of test weight has also appeared. This measurement is not unfamiliar in the case of other cereals, such as wheat and barley, but it has not been applied widely in maize. It is likely for this reason that we have such little information and research available on this topic. In this study, we show the current state of this field with references from domestic and international literature.
The density of maize is the weight of a particular volume and the most frequent unit is the test weight (kg/hl). This physical quality factor plays important roles in the storage, transport and mill industries. The value of test weight is influenced by many factors. The most important ones are the moisture content of grains, drying temperature, drought, precipitation, early frost, and the hybrid characters of a given genotype (grain type, FAO number). In general, the grain with higher moisture content has lower test weight and the higher temperature during (above 82°C) desiccation also leads to unfavourable values. Factors such as a drought interval after flowering, early frost in the case of hybrids with higher FAO numbers, injuries by insects, as well as fungal infections also influence the structure and moisture content of the maize grain.
In the future, broader studies (hybrid testing, application of new agrotechnical elements) will be needed for understanding of the factors effecting test weight. -
Results of weed surveys in greening plants
53-57Views:106Greening crops play an essential role in Hungary's agriculture. Weeds can also cause many problems during the development of greening plants. Our research aimed to evaluate the weed control properties of greening crops sown with different germination rates. Analysis of the effect of crop rotations on weed density. Comparison of weed growth in control, fertilised and greened areas. In October of 2021, a weed survey was carried out in lupin (Lupinus albus L.), common vetch (Vicia sativa L.), oil radish (Raphanus sativus var. oleiferus L.) and buckwheat (Fagopyrum eculentum Moench). During the weed survey, we determined the different weed species and their abundance. In terms of seed rates, the higher seed rates for lupin, oil radish, and buckwheat may be worth choosing for weed suppression. Plots in rotation III had the lowest weed incidence of all greening crops. The probable reason for this finding is that there was no prior greening in rotation III. For greening, the choice of buckwheat and oil radish will result in higher weed pressure. The most important weeds were the cereals sown before the greening crop. Fertilised plots had minimally fewer weeds than control plots. Research results show the difficulties of weed control in herbicide-free greening crops.
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Possibilities of selection and production of blue and ornamental corn (Zea mays L.)
15-19Views:117The cultivation of alternative crops has an important role in world agriculture. Their market share is continuously growing in the food industry sector. In the present study, we show the cultivation and breeding perspectives of ornamental and blue corn. There exists possibility to cultivate ornamental corn in Hungary. There are many cultivars of ornamental corn, with various kernel colors, husks, stalks and leave colors. Blue corn is unique among other corns. Blue corn higher in protein, iron and zinc than commercial dent corn. Pests affective ornamental and blue corn are the same as those of other commercial corns. Ornamental and blue corns need to be grown a minimum of 500 m away from commercial yellow dent corn to minimize any cross pollination which may result in off-color kernel. Our aim in the presentation of this review was to broaden Hungarian literature.
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Experiments on the Nutrient Removal and Retention of an Integrated Pond System
18-23Views:205A combined intensive-extensive fishpond system developed for the purification and re-use of intensive fishpond effluent water was studied during a three-year experimental period. The investigated pond system consists of five small-size intensive culture ponds of 1 ha total water surface area with 1.5 m water depth and a 20 ha extensive culture pond with 1.0 m average water depth. The water was recirculated between the intensive and extensive ponds with around 60 days retention time in the extensive treatment pond.
Carbon, nitrogen and phosphorus budget and water purifying capacity were described and evaluated by means of regular measurements of nutrient concentrations in the water and sediment. During the three-year test period, 81.5% of organic carbon, 54.7% of nitrogen and 72.2% of phosphorus were retained by the system as a percentage of the total input of each nutrient. A significant amount of the total nitrogen input was removed by the harvested fish, which was much higher than in traditional fishponds or intensive fish culture systems. The efficiency of nutrient removal is clearly indicated by the 27.3% nitrogen assimilation.
Only a small percentage of the total nutrient input was discharged into the environment during fish harvest, which was 9.0% for organic carbon, 13.2% for nitrogen and 12.1% for phosphorus. The combination of intensive and extensive fishponds with water recirculation resulted in significant reduction of nutrient discharge into the surrounding aquatic environment, primarily due to the high nutrient processing and retention capacity of the extensive fishpond ecosystem. -
Evaluation of blue and red corn landraces
239-243Views:135Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
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Evaluation of the role of common vetch (Vicia sativa L.) green manure in crop rotations
161-171Views:206Common vetch (Vicia sativa L.) is an annual legume, grown as green manure provide rapid soil cover, can increase soil moisture and organic matter content and reduce soil erosion during fall. During the fallow period, legumes grown as catch crops are known by releasing large amounts of mineral nitrogen (N) for the subsequent crop. By taking advantage of these benefits, it is possible to increase the yield of the next crop in an environmentally friendly and sustainable way. The goal of this study was to determine the value of common vetch as a green manure, considering its effect on soil conditions and the yield of next crops. We examined three different common vetch seed rate as a green manure in a crop rotation with triticale, oat and corn. Next to the green manured treatments, we used fertilized and bare fallow control treatment for comparison. In our study we evaluated the aboveground biomass weight of spring vetch green manure and its effect on the moisture content of the soil. We examined the green manure’s effect on the next crops plant height and yield. We found that the moisture content of the green manured plots was significantly higher during summer drought. On the green manured plots, 37.9% higher triticale yield, 50% higher oat yield and 44% higher corn yield were measured compared to the control plots. The insertion of spring vetch green manuring into crop rotations could be a good alternative to sustainable nutrient replenishment methods. It can be used to reduce the input needs of farming, reduce carbon footprint, contribute to the protection of soils and increase the organic matter content of the soil.
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Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:119We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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The father of the term ‘biotechnology’ was Hungarian: The forgotten works of Károly Ereky
107-110Views:374In the Nature, Robert Bud gave an account of the fact that the generator of the concept "biotechnology" was the Hungarian Karl Ereky who, in his book published in Berlin in 1919 entitled "Biotechnologie der Fleisch-, Fett- und Milcherzeugung im landwirtschaftlichen Grossbetriebe" ("Biotechnology of Meat, Fat and Milk Production in a Large-Scale Agricultural Farm"), disclosed his observations and new views in that regard. Later on, Ereky's two essays, published in German language again, and other contemporary German sources have been processed which have confirmed the assumption whereas – in scientific terms – the further first applications of the expression "biotechnology" can be attributed to Ereky's works. Recently, we have explored and found biographical sources and documents which had been published by Ereky, which, however, have already been forgotten, by dint of which the story of origin of the first concept "biotechnology" can as well be clarified in a factual manner.
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Phenometric studies on stalk juice and sugar contents of silo sorghum types
41-49Views:92Bioenergies (among them e.g. bioalcohol) can be solutions for the replacement of fossil fuels. For its production, plants with high sugar or starch content can be used. Juice pressed from the stalk of sugar sorghum has high sugar content (14-17%) that makes it suitable for bioethanol production. During our experiment, we examined 53 restorer male lines; among them 22 were silo type sugar sorghum. We studied the following traits: plant height, breeding time, level of foliation, stalk diameter, characteristics of stalk medulla, juice content of stalk, sugar content of stalk juice. According to examined characteristics, we selected six restorer male lines for studies in the forthcoming years: RL 1, RL 2, RL 3, RL 4, RL 5, RL 9, RL 12, RL 15, RL 18. Their stalk medullas were wet, stalk diameters were medium-thick, sugar contents of juices varied between 17 and 24% at the end of milk mature. Harvest was made in September, they can be classified into early maturation group. Male sterile female lines were the following: SL 1, SL 2, SL 3, SL 4, SL 5. The maintainer male lines were: CL 1, CL 2, CL 3, CL 4, CL 5. In Hungary, there are only a few male sterile female
lines, so we will use these lines for hybrid production during the next years. -
The effect of diet composition, a probiotic and a symbiotic treatment on the ileal microbiota composition of one-week-old broiler chickens
213-220Views:194A healthy microbiota present in the small intestine contributes significantly to small intestinal function, including digestion, nutrient absorption and health. The current study investigated the effects of a prebiotic, a probiotic and a symbiotic supplementation on ileal microbiota composition of broilers at 7 days of age. In a total of 574 male Ross 308 day-old chickens were divided into four groups using six replicate pens and 24 chickens per pen. A maise-soybean based control diet (C), a control diet supplemented with probiotics (Broilact; Br), a control diet supplemented with symbiotic (inulin, yeast, Bacillus subtilis; Sy) and a wheat based diet supplemented with wheat bran (W) were formulated. On day 7 of life, two chickens per pen were slaughtered and ileal chymus samples were collected. For microbiota analysis 16S rRNA (V3-V4 region) gene targeted Illumina MiSeq sequencing was used. Feeding all diets supplemented increased the diversity to varying degrees compared to the control (C) diet (p=0.006). As a conclusion, all supplementation substantially influenced ileal microbiota of broiler chickens at an early age. All these results could offer some information for the future study on the relationship between early intestinal microbiota and the compounds of the feed.
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Survey of the environmental impacts of pond fish farming
19-24Views:94The aim of this study was to survey the nitrogen, phosphorus and organic matter loads and the discharge of fishponds. The inputs and outputs of nutrient amounts of fishponds and their sources are described. The impact of a fishpond on the nutrient loads of receiver waters was determined. The investigations of this study were to determine and evaluate the nitrogen, phosphorus and organic matter budget of fishponds representing different technologies and areas.
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Harvesting system established for the utilisation of Miscanthus sinensis ‘tatai’ “energy cane” in biomass power plants
143-150Views:158The increasing demand for energy worldwide and the resulting environmental impacts of fossil fuels forced many countries to turn to renewable energy resources as a clean and sustainable alternative. More than a third of Europe’s binding renewable energy source target of 20% by 2020 will come from solid biomass for electricity and heating according to the National Renewable Energy Action Plans submitted by member states of the European Union (EU) to the European Commission. To achieve this goal long-term yield studies in renewable energy plants are important to determine mean annual biomass and energy yield, and CO2 emission. Field experiments worldwide and also in Europe have demonstrated that Miscanthus, a fast-growing C4 rhizomatous grass can produce some of the highest biomass and energy yield per hectare of all potential energy plants. Miscanthus is a plant that originates from the southern slopes of the Himalayas. It was bred for the Hungarian climatic conditions in 2006 under the name of Miscanthus sinensis ‘Tatai’ (MsT). The species has high frost and drought tolerance and high energy value. This is why there is growing demand for the biomass (lignocellulose) produced by growing this plant. The biomass, produced from the high yield energy reed, can be transported to power plants in large quantities, in forms of bales. Its household consumption is not yet significant. This study presents the external features, characteristics, propagation and plantation process of MsT energy reed. The study also demonstrates the harvest technology of the species worked out between 2009–2012 in Tata, Hungary and the options of supplying to biomass
power stations.
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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:111Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase