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Ovarian performance of Barbados Blackbelly and Dorper crossbred ewes in the mirror of body conditions
23-26Views:92The aim of examination was to detect the puberty and to follow the ovarian cycle of Barbados Blackbelly×Dorper ewes in connection with their body conditions and the effects of meteorological parameters. The blood samples had been taken from 17th of November 2008 untill 08th of June 2009 for analyzing the progesteron concentrations, establishing whether they showed perpetual ovarian performance or not. The end of examination
was timed due to releasing the ram in middle of May 2009.
The daily avarage bodyweight-growth is well featured by the bodyweight tendence. The body conditions were evaluated by ultrasound tissue examination.
During the full length of the examination the avarage daily temperature, the avarage humidity level and also the number of daily sunlighted hours were served by the Agrometeorological Observatory of the University of Debrecen.
The bodyweight tendence and the meteorological parameters were correlated to the breeding season of ewes. In conclusion the bodyweights and their tendences at the young age and the body condition parameters measured at certain anatomical areas can influence the length of the breeding season of the ewes. However the number of daily avarage sunlighted hours, the daily avarage temperature and also the humidity did not effect the cyclic ovarian perfomance of the Barbados Blackbelly×Dorper ewes in Hungary. -
Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
61-64Views:169In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.
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Evaluation of technologies and resources from the spatial and social aspect
73-75Views:212The basic functioning of the world is mostly led by negative trends. One of the fundamental reasons for this phenomenon is that the different technologies (hardware, software, resources) do not act properly and in the proper structure by means of the produced products and services. Therefore, the examination of technologies and resources is a key question of our world and development from various aspects. Technologies convey all positive and negative impacts which can be regarded as the fundamental reasons and causes of harmonious or disharmonious development. For this reason, the role of technologies in development and harmonious functioning is of paramount importance.
As a final conclusion, we established that the different technologies (hardware, software, resources) are interpreted as impact conveyors which predominate by means of products and services in a way that their relation system is determined. By using the thermodynamic interpretation which is the basis of the functioning of the world, we built a system consisting of 14 elements to evaluate different technologies, to interpret its functioning and to develop positive interactions and trends.
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Examination the stresseffect of litter’s let-together system (“kindergarten”) by measuring faecal cortisol metabolites of sows and piglets
87-90Views:175Piglets in commercial intensive pig husbandry are often abruptly weaned between 3 and 4 weeks for economic reasons. The process of weaning is a multifactorial stressor, in which nutritional, social, physical and psychologic stressors are combined. Piglets are often exposed to unfamiliar piglets around weaning which results in a period of vigorous fighting. Stress plays an important part in welfare research. Traditionally glucocorticoids are measured in blood samples but their use is often limited as the act of sample collection may stress an animal. Measurement of faecal cortisol/corticosterone metabolites is a non-invasive method for evaluation adrenocortical activity. The aim of this study was to examine the effect of litter’s let-together system (“kindergarten”) in the farrowing house by measuring faecal cortisol metabolites. According to our results the “kindergarten” system has no stress effect on sows and piglets, respectively.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:212The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Methodological questions of the process structure benchmarking analyses planned in relation to the international Airport of debrecen
147-154Views:172From June 2012 continuous traffic has begun between London Luton and Debrecen at the International Airport of Debrecen, which is considered a milestone in the growth of passenger traffic. The goal of this research is to demonstrate the fact that growt of passengers depends, on the visitors satisfaction with services used by them. The research of the customer service requires to develop process structure that includes the services used by the passengers from arriving at the airport to leaving the airport. A process structure like this allows us to carry out a benchmarking method to compare the passaenger services of other cities. To build up a structure we have to make segmentation according to the travellers’ motivation because they use different services according to their motivation. A questionnaire of customer satisfaction will be presented which results are the basis of the creation of benchmarking and creation of process structures.
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Effects of soil compost application on the leaf surface area per running meter of apple (Malus domesticaBorkh.) leaves cultivated in organic and integrated orchard
111-116Views:146The effect of different compost doses on the nutrient contents of the soil (in depth of 0–30) were measured in an (organic and integrated) apple orchards. The leaf surface area per running meter shoot for certain apple species (Golden Delicious and Pinova) were determined.
The field experiments were conducted in the Research Field of Debrecen-Pallag, University of Debrecen, the analysis of the soil and plant samples took place in the laboratories of the Institute of Agricultural Chemistry and Soil Science.
The compost treatments increased the amount of the examined nutrients in the top 30 cm of soil originated from organic and integrated orchard. Changes in rainfall were observed in the trends of the leaf surface area of the two apple varieties. 2010 was rainy and 2012 was drier. The leaf surface area of the Pinova increased better under the precipitation/year effect, than in case of the nutrient supply treatments. The opposite was observed in case of the Golden Delicious. -
Survival analysis of sow longevity and lifetime reproductive performance – Review
75-80Views:296Sow longevity plays an important role in economically efficient piglet production. Improving sow longevity results increase in the productivity and profitability of a sow herd. Longevity is a complex trait with many factors that can contribute to a sow having a long and productive life. Not only the sow’s genetics, but also nutrition, environment and the management policies are important. In addition, the removal of non-productive sows along with the introduction of replacement gilts is an essential part of maintaining herd productivity at a constantly high level. The objective of this paper was to summarize the current knowledge about the sow longevity and lifetime performance values, as well as, discusses the survival analysis methods for sow longevity traits. This method can estimate the hazard rate indicating proportional risk of sows being culled at any given time, in addition, it is able to investigate the effects of different factors on longevity. Identifying factors that influence the longevity could assist many commercial pig producers in becoming more efficient.
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Sweet cherry fruit quality by half-intensive orchard management
76-79Views:95We made our study on fruit quality in the summer of 2005 on 6 cultivars („Bigarreau Burlat”, „Germersdorfi 3”, „Germersdorfi 45”, „Katalin”, „Linda” and „Van”) in a half-intensive sweet cherry orchard in Siófok. We measured fruit diameter, fruit width, fruit height, stem length and stem weight, fruit and pit weight and total soluble solids content. There were large differences among cultivars in the datas. These differences are due to genetic fruit-characteristics, because all other conditions were the same. We harvested fruits by 3 cultivars two times to check their characteristics on two ripening phases. We examined on these cultivars all the above listed fruit quality parameters. With these data we can be informed about the role of later than optimal harvesting time and how big an effect it has on fruit quality.
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New methods of compost homogeneity determination in sewage sludge based compost prisms
49-52Views:162There is no effective method of homogeneity measurement of compost prism. The most frequently used technology is the examination of the particle distribution. This method needs a lot of time and large number of samples. The aim of our research is establishing different effective methods to determine the homogeneity of compost prisms. During our work, we examined the homogeneity of a prism made of sewage sludge and saw-dust mixture.
The measurements were based on the different properties of raw materials. According to this we examined the homogeneity by moisture content, heavy metal content and gas distribution measurements.
The most effective method is the measurement of gasconcentration. Although gas-concentration measurements it need special equipment it has more advantages than the other methods. The examination of gas-distribution compensates the problem of sampling because the measurement is direct. It provides the opportunity to estimate the amount of emitted toxic gases and to determine the maturity of the compost and the effectiveness of the degradation. -
The effect of water supply and crop year on the yield potential of sweet maize (Zea mays L. convar. saccharata Koern.) hybrids with different genotypes
203-210Views:182The successfulness of crop production is significantly affected by not only the the average yields that provide cost effectiveness, but also the success of striving for yield safety, therefore, varieties and hybrids tolerant to environmental stress factors are worth being included into the sowing structure. Our aim was to further the decision making of producers in prepaering the right sowing structure by the evaluation of sweet maize hybrids’ tolerance to excess rainfall.
We performed our examinations in an extremely wet year (2010) on chernozem soil on three sweet maize hybrids (GSS 8529, GSS 1477, Overland) in 12 replications. Comparing the yields of 2010 with those that can be expected under optimal rainfall conditions, we showed that the examined hybrids react to the amount of rainfall higher than their needs with yield depression. The excess rainfall tolerance of the examined hybrids is different in the case of each hybrid. -
Effect of the media on morphology of Cryphonectria parasitica (Murr.) Barr isolates and their Vegetative Compatibility Groups
38-42Views:164The most dangerous pathogen for the European and American chestnuts is the blight fungus Cryphonectria parasitica (Murrill) Barr. Short after its introduction a big number of chestnut trees were destroyed on the infested area. The control could be really complicated, because of the numerous vegetative compatibility groups of the fungus. There is a type that carries a mycovirus viz. hypovirus in the cytoplasm. We are able to control effectively this pathogen by using mycovirus-carrying strains (called hypovirulent fungal strains also). In laboratory it is easy to multiply the virulent and the hypovirulent strains of the fungus but do not easy to differentiate colonies visually on simple PDA medium. During our research, we tested different types of media, based on potato and chestnut bark extract respectively. It was observed that on potato medium the virulent strains produce more orange pigments. So it is more easy to differentiate virulent or hypovirulent isolates of chestnut blight fungus based on colony colour and morphology.
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The effect of crop protection and agrotechnical factors on sunflower in the Hajdúság region
39-46Views:92Extreme weather conditions are becoming more and more frequent in the crop years, thus increase the risk of sunflower production.
The objective of researches into plant production is to minimize these effects as much as possible. In this sense, the optimization of
agrotechnological factors is of high importance. Within these factors, the appropriate crop technology (sowing time, crop density)
and optimized, rational crop protection technologies are important, especially in the highly sensitive sunflower cultures. The effect of
sowing time, crop density, and fungicide treatments on the yield of sunflower hybrids was analysed in different crop years in 2008
and 2009. In each case, the infection was highest with the early sowing time and at the highest crop density level (65000 ha-1). When
one fungicide treatment was applied, the rate of infection decreased compared to the control treatment. The further decrease of the
infection rate was less after the second fungicide treatment.
In the humid year of 2008 the crop yield was the highest at 45000 ha-1 crop density level in the control treatment and at 55000 crop
ha-1 crop density level when fungicides were applied. In the draughty year of 2009 the maximum yield was gained at 55000 ha-1 crop
density level in the control treatment and at 65000 crop ha-1 when fungicides were applied. In 2008 and 2009 as regards the crop
yield, the difference between the optimal and minimal crop density levels was higher in the fungicide treatments than in the control
treatment (in 2008: control: 517 kg ha-1; one application of fungicides: 865 kg ha-1; two applications of fungicides: 842 kg ha-1), (in
2009: control: 577 kg ha-1; one application of fungicides: 761 kg ha-1; two applications of fungicides: 905 kg ha-1).
In each and every case, the first treatment with fungicides was more effective than the second. In 2008, the highest yield was
obtained with the third, late sowing time in each fungicide treatment. The differences between the crop yields with different sowing
times was less than in 2009, when the results of the second treatment exceeded those of the first and third treatment in each case. -
Movement of Particles in the Air
22-26Views:108The physical characteristics of particles (seeds and fertilisers) can strongly influence their movements both in seeding and spreading machines and in the air. It is therefore essential to study these particles when constructing such machines. In this respect the size, shape, coefficient of friction and aerodynamic resistance of particles are of great importance.
Due to their irregular shape, determining the size of particles is a troublesome process. A precise description of particles has to include several sizes and can be obtained from their screen size. Many physical properties of particles are relevant during movement in the air, but the aerodynamic resistance coefficient is the most important (Hofstee et al., 1990). Two types of wheat and four types of fertiliser particles were investigated (supported by the National Scientific Foundation OTKA, T-026482). An elutriator was designed and constructed (Csizmazia et al., 2000), in which an airflow is supplied by a centrifugal fan. Air velocity was measured with a thermal sensor. Particle sizes, mass and terminal air velocity were measured. The influence of the aerodynamic resistance coefficient on the particles’ motion is also discussed. -
Molybdenum - accumulation dynamics of cereals on calcareous chernozem soil
81-85Views:131This work is about the molybdenum-accumulation of cereals analyzing soil and plant samples from a field experiment set in
Nagyhörcsök by Kádár et al. in 1991.
In this long-term field experiment different levels of soil contamination conditions are simulated. Soil and plant samples were collected
from the experiment station to study the behaviour of molybdenum.
In this report results of maize, winter wheat, winter barley and soil analysis are presented. The conclusions are as follows:
– Analysing soil samples from 1991 we have found that roughly half of the molybdenum dose applied is in the form of NH4-acetate+EDTA soluble
– Comparing element content of grain and leaf samples we have experienced that molybdenum accumulation is more considerable in the vegetative plant parts
– Winter wheat accumulated less molybdenum then maize in its vegetative parts. Comparing molybdenum content of winter wheat to winter barley we found that the concentration of the element in wheat was lower by half than in the winter barley. It seemed that molybdenum accumulated to the least degree in winter wheat. -
Spectral analysis of stress symptoms caused by apple powdery mildew (Podosphaera leucotricha
83-88Views:191An orchard can be examined on the basis of spectral data, using methods with which the reflected radiation can be divided into a large number of (several hundreds) small spectral channel (some nm). Calculated on the basis of such hyperspectral data from different index numbers the water supply of foliage conditions can be well characterized.
The research site is an intensive apple orchard, which located in University of Debrecen, Agricultural Sciences Centre, Farm and Regional Research Institute at Pallag. During my experiments the preliminary evaluation of spectral, non-invasive measurement method are carried out for detecting stress symptoms caused by Podosphaera leucotricha.
Based on the results narrow band greenness indices (NDVI705, mNDVI705, mSR705 and REP) can be used for determination of diseased canopy and for the detection of stress symptoms of Podosphaera leucotricha,. These statements can be utilized in precision plant protection systems, since it can be a basis for such integrated active sensors with LED or laser light source, measuring reflectance at the certain spectral range, which can facilitate real time status assessment of orchards and can control precision fungicide utilization.
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Modelling forestation alternatives
35-41Views:226Agroforestry systems are part of the history of the European Union rural landscapes, but the regional increase of size of agricultural parcels had a significant effect on European land use in the 20th century, thereby it has radically reduced the coverage of natural forest. However, this cause conflicts between interest of agricultural and forestry sectors. The agroforestry land uses could be a solution of this conflict management. One real – ecological – problem with the remnant forests and new forest plantation is the partly missing of network function without connecting ecological green corridors, the other problem is verifiability for the agroforestry payment system, monitoring the arable lands and plantations.
Remote sensing methods are currently used to supervise European Union payments. Nowadays, next to use satellite imagery the airborne hyperspectral and LiDAR (Light Detection And Ranging) remote sensing technologies are becoming more widespread use for nature, environmental, forest, agriculture protection, conservation and monitoring and it is an effective tool for monitoring biomass production.
In this Hungarian case study we made a Spatial Decision Support System (SDSS) to create agroforestry site selection model. The aim of model building was to ensure the continuity of ecological green corridors, maintain the appropriate land use of regional endowments. The investigation tool was the more widely used hyperspectral and airborne LiDAR remote sensing technologies which can provide appropriate data acquisition and data processing tools to build a decision support system.
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Results of weed surveys in greening plants
53-57Views:86Greening crops play an essential role in Hungary's agriculture. Weeds can also cause many problems during the development of greening plants. Our research aimed to evaluate the weed control properties of greening crops sown with different germination rates. Analysis of the effect of crop rotations on weed density. Comparison of weed growth in control, fertilised and greened areas. In October of 2021, a weed survey was carried out in lupin (Lupinus albus L.), common vetch (Vicia sativa L.), oil radish (Raphanus sativus var. oleiferus L.) and buckwheat (Fagopyrum eculentum Moench). During the weed survey, we determined the different weed species and their abundance. In terms of seed rates, the higher seed rates for lupin, oil radish, and buckwheat may be worth choosing for weed suppression. Plots in rotation III had the lowest weed incidence of all greening crops. The probable reason for this finding is that there was no prior greening in rotation III. For greening, the choice of buckwheat and oil radish will result in higher weed pressure. The most important weeds were the cereals sown before the greening crop. Fertilised plots had minimally fewer weeds than control plots. Research results show the difficulties of weed control in herbicide-free greening crops.
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The effect of keeping technology on the microbiological status of raw milk
67-75Views:118The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,
deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.
The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.
During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.
The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and therefore
equipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk. -
Evaluation of population parameters and biometric data of an Eurasian Collared Dove (Streptopelia decaocto Friv.) population in the Great Plain of Hungary
89-92Views:339The Eurasian Collared Dove shows different patterns in density all over Hungary. In some cases, the population has decreased to a lower level, but there are habitats where the population remained stable. Several factors were taken into consideration during the evaluation of this phenomenon. These publications state that the increased urbanization of corvids, the lack of food sources, and the changes in nesting sites caused the decrease in numbers. During our research, we intend to contrast the differences in population structure of Collared Doves that differ from each other in the density of predators presented at nesting sites. As a first step, in this paper, numerous samples (n=118) were taken from a population that is less influenced by corvid species’ nest predation. Our prediction was that the population parameters remained at the level of 1980’s, in which the population of Eurasian Collared Dove has reached its maximum, according to further publications.
During the evaluation process, our data were collected from hunting bags near Nyíregyháza. Results were compared with former publications. We stated that the population structure, such as age and gender distribution remained stable in the evaluated population. In addition, morphometric factors of the collected individuals were also measured, such as weight, wing length, 3rd primary feather, and tail length, which were compared with former surveys. During statistical tests no significant difference was revealed among population parameters, but there were significant differences between most of the morphometric data, (p>0.05).
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Responses of pea (Pisum sativum L.) to the rising atmospheric concentration of carbon-dioxide
185-188Views:202The atmospheric concentration of carbon dioxide increases from decade to decade in increasing pace. In 1957, atmospheric carbon dioxide levels were around 315 ppm, while in 2012 it amounted to 394.49 ppm concentration. In parallel, the global temperature is rising,which is projected to average 1.5–4.5 °C. The carbon dioxide concentration is a key factor – in interaction with the light – affects the plant's photosynthesis. Among the various factors significant interactions prevail: environmental factors affect - the growth and the development of plants, leaf area size and composition, the function of the photosynthetic apparatus, the duration of growing season.
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The Effect of Sunburn on Fruit Quality of cv. Idared Apple
23-31Views:102The aim of this study is to investigate the effect of sunburn injury on fruit quality parameters (colour-coverage, depth of affected tissue, fruit flesh firmness, soluble solids content) of apple.
The symptoms of sunburn injury appeared in concentric ring shape, differed from each other and surface colour-coverage. This can be due to the ratio of the injury. The authors observed the following colours on the fruit surface (from the epicentre of the blotch on the transversal diameter of the fruit) dark brown (strongly affected), light brown (moderately affected), pale red transition (poorly affected), red surface colour-coverage (not affected).
Sunburn of apple fruits is a surface injury caused by solar radiation, heat and low air relative humidity that in the initial phase results in a light corky layer, golden or bronze discolouration, and injuries to the epidermal tissue, in the surface exposed to radiation. Thus it detracts from its appearance, but in most cases, it would not cause serious damage to the epidermal tissue. The depth of affected tissue is not considerable, its values are between 1.5-2.0 mm generally. It is commonly known that the tissue structure of apple fruit is not homogeneous. Accordingly, the degree of injury shows some differences under the different parts of the fruit surface.
On the basis of flesh firmness research, the authors established that the measure of flesh firmness of the affected part of apple fruit increases with the effect of sunburn. The consequence of this is the suffered plant cells will die, the water content of this tissue decreases and the fruit gets harder. This water-loss caused the increase of soluble solids content. -
Application of advanced environmental assessment methods in orchard management
221-225Views:137Our reseaches were carried out in apple and pear orchards at Farm and Regional Research Institute in Pallag of the University of Debrecen and Pear Gene Reservoir in Újfehértó. Aim of this study is to interpret and analyse field studies with the aim of a GIS based database. Furthermore, beside field measurements, airborne and field hyperspectraldatacollection and analysis were also made to facilitate special watermanagement and irrigation related surveys. The integration of unified, geoinformatics systems with high spatial resolution and calibrated airborne hyperspectral data are appropriate tool for decision support systems, which support the continuous update and actualization of the changing cropping data, the analysis of cropping results in a unified complex data system, the acquiring of agro environmental subsidies, the establishment of monitoring system, and the optimization of irrigated fruit production.
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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:284Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Association analysis of TNNI1/XbaI polimorphism on carcass quality in hybrid pigs
59-62Views:174The contractile protein, which is encoded by troponin I 1 (TNNI1) gene, is located on the thin filaments of slow fibres in striated muscle. TNNI1 protein is a part of the troponin complex which plays an important role in regulation of muscle contraction by preventing actin-myosin interaction in absence of calcium. According to biological role, this gene can be potential marker for meat production related traits. The aim of this study is to define whether the previously reported gene polymorphism (EU743939:g.5174T>C) is connected with the slaughter traits measured in a standard slaughterhouse of the examined four-line European hybrid. The study included data from 404 gilts and barrows from 2 different samples. The polymorphism was detected using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method with XbaI restriction enzyme. In this study the allele frequencies were found as follows: C: 0.84 and 0.808; T: 0.16 and 0.192. Based on result of the present study no significant impact of polymorphisms on production parameters was found.