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• Overwintering capability and spring population size of honeybee colonies (Apis mellifera L.) in Hungary

  153-156.

  I. Ludányi

  Views:

  107

  Honeybee races and ecotypes of different genetic background have different population development in spring. Some of them can reach the necessary population size by the beginning of Robinia pseudoacacia (black locust) blooming period. There were significant differences in the spring population development between the colonies of different genetic background. The Italian races (A. m. ligustica) and their cross-breeds over-wintered poorly in Hungary, their spring population was low and they collected small amount of Robinia honey. The Austrian improved Carniolan (A. m. carnica) colonies over-wintered well, they had the largest spring population in both years. There was no significant difference between the size of the spring population of the same colonies of different genetic background in 1995 and 1996. The rate of the population development of the colonies was different in the two examined years. There was strong correlation (r = 0.8) between the spring population size and the Robinia honey yield, and between the mid-April population size and the Robinia honey yield of the colony groups of different genetic background. Spring population size also important in the effective pollination of fruit tree species that bloom earlier than the black locust trees.

   

• Development of microsatellite markers for Rhodiola rosea

  37-42.

  A. Veress

  B. Lendvay

  A. Pedryc

  Z. György

  Views:

  202

  Rhodiola rosea L. is an important adaptogen medicinal plant. In this study two new microsatellite markers were developed. The assessment of the genetic diversity of R. rosea has recently started with molecular markers, but only a few species-specific microsatellite markers have been published so far. However the small number of markers allows only a limited insight into the genetic variability of the species therefore the aim of our work was to develop new microsatellite markers for R. rosea with a microsatellite enrichment library technique. Genomic DNA was cleaved with an endonuclease enzyme followed by adaptor ligation and PCR amplification. DNA fragments that contained microsatellites were first isolated using a biotin-streptavidin linkage based magnetic selection and then cloned into plasmids. Out of forty-three sequenced clones three contained  microsatellites, in these cases primers were designed for the amplification of the microsatellite repeats. The newly developed primer pairs were tested on individuals from distant R. rosea populations and the variability of the amplified fragments was estimated by fragment-length analysis. The locus RhpB14a was found to be monomorphic while RhpB14b and RhpB13 were polymorphic. As a result of the present study, two novel variable microsatellite loci were identified in the genome of R. rosea.

• Studies on the Tobamovirus resistance of the pepper (Capsicum annuum L.) cultivar Greygo

  71-75.

  P. Salomon

  G. Venczel

  Views:

  144

  Resistance of the Hungarian pepper (Capsicum annuum L.) cultivar "Gre_.ygo" to Tohamoviruses has been investigated. All plants of the population of Greygo proved to be resistant to tobacco mosaic and tomato mosaic viruses (TMV, ToMV), both represent the pepper pathotypes P_o of Tohamoviruses. Individuals of Greygo, however, were found to be susceptible to pathotypes P₁₂ and P₁₂₃ of pepper mild mottle virus (PMMV). When inoculated with the XM isolate of dulcamara yellow fleck virus (DYFV, pathotype P1) the population of Greygo segregated in resistant and susceptible plants. These results as well as inoculations of the progenies of three TMV resistant plants clearly showed, that besides the resistance allele Li the cultivar Greygo possesses also an another allele. This allele, provisionally marked by L²g behaves like to the allele L² characteristic to Capsicum frutescens cv. . Tabasco. Determination of the identity of the allele L²g to the allele L² needs further genetic and pathological informations. Relations between the Tohamoviruses pathogenic to pepper and the alleles of the resistance gene L are outlined for the discussion.