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• Certification programme for production of virus-free propagating material of grapevine and its results in Hungary

  39-43.

  J. Lázár

  J. Mikulás

  G. Farkas

  M. Kölber

  Views:

  139

  In Hungary, detection of virus and virus-like diseases of grapevine began in 1960's at the Research Institute for Viticulture and Enology by János Lehoczky and his colleagues. At present, sixteen virus and virus-like diseases of Vitis vinifera are known to occur in Hungary.

  Regular virological screening of grapevine varieties started in 1972. The present system of screening (visual selection, indexing, ELISA) has been established using methods with continuous improvement according to recommendations of international organizations.

  In the first year symptomless grapevine plants are selected and marked during surveys carried out twice in the vegetation period: at about flowering and in the second half of September. At the first selection time plants are sampled for ELISA.

  In the spring of the second year, overwintered canes are checked by woody indexing on 8 indicator species in the field.

  In the third and fourth years the nursery is evaluated twice again. At the end, the marked grapevine plants, giving negative results on all indicators in every case, are considered virus-free.

  In autumn of the fourth year, the virus-free material is planted out under screenhouse and also in a special mother block (nuclear stock) for maintenance and propagation.

  Mother blocks of virus-free scion varieties have been established on 2 ha and those of rootstock varieties on 0.5 ha planted with the following number of varieties included in the national list: 71 European scion — and 12 rootstock varieties or variety candidates/clones. It is necessary to increase the area of Pre-base, Base and Certified stocks exclusively with tested virus-free (clean) material.

• Translocation of diquat dibromide

  105-107.

  J. Mikulás

  J. Lázár

  G. Kazinczi

  Views:

  117

  The aims of our work were to answer the following questions: 1. Diquat dibromide at different concentrations is a contact or systemic herbicide? 2. if translocation occurs what is the extent and direction of it. 3. by what concentration it is translocated in hydroponics. It has been stated that diquat dibromide at different dilutions (40-5%) had systemic effect in Robinia pseudo-acacia in the fields. Its translocation has been occurred apically first, and later basipetally. The extent of translocation depended on the concentration. In hydroponics diquat dibromide has crytallized on the leaf surface of Galinsoga parvif lora and all concentrations (40-0.078%) proved lethal. Recognition and application of systemic characteristics of diquat dibromide is reported here for the first time. Improvement of application method is in the focus of our future work.

• Research Note virus transmission by grape phylloxera (Daktulosphaira vittfoliae Fitch)

  73-74.

  L. Tóth H.

  Mikulás J.

  Kocsis L.

  Views:

  124

  Grape phylloxera (Daktulosphaira vitifoliae FITCH) infests immature roots of both Vitis vinifera L. and phylloxera resistant rootstocks. A capability to transmit viticultural viruses would make grape phylloxera a phytosanitary threat even under conditions where direct damage by the insect is not likely. We tested the hypothesis that phylloxera could transmit grapevine fanleaf virus (GFLV) by planting infected and non-infected vines in common 10 liter pots and infecting roots of the infected vine with grape phylloxera. In this test infection of a previously non-infected plant in the absence of nematode population suggests that grape phylloxera is a vector of GFLV.