Search

Search Results

• Influence of different growth regulators on the in vitro morphogenesis of an ornamental variety of carnation

  55-57.

  Zs. O. Kiss

  A. Mándy

  Views:

  227

  Callus formation, as a prerequisite for the induction of somaclonal variability, was achieved successfully with certain molar ratios between 2,4-dichlorophenoxyacetic acid and benzyladenine. Regeneration of new plants from shoot apex meristems could be significantly improved by the combined addition of very low amounts of indolebutiric acid, benzyladenine and gibberelic acid, dissolved in the Murashige-Skoog nutrient medium. These in vitro treatments may contribute to a more efficient micropropagation of the Rimini variety of carnation.

• The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'

  77-83.

  M. Ördögh

  E. Jámbor-Benczúr

  A. Tilly Mándy

  L. Lelik

  Views:

  363

  The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.

• Influence of antiobiotics on NAA- induced somatic embryogenesis in eggplant (Solanum melongena L. cv. Embil)

  88-95.

  E. A. T. Picoli

  W. C. Otoni

  P. R. Cecon

  M. Fári

  Views:

  342

  The influence of increasing concentrations of naphthaleneacetic acid and the antibiotics cefotaxime, timentin, kanamycin, and hygromycin on eggplant (Solantun melongena L. cv. Embil) somatic embryogenesis was investigated. Cotyledon explants were excised from 16 to 20 days old in vitro grown seedlings. NAA promoted somatic embryogenesis, although its concentrations had no influence on the mean number of embryos. Callusing decreaSed significantly with increasing NAA concentrations. Morphogenesis was stopped with 50 to 100 mg L^-1 kanamycin and 7.5 to 15 mg L^-1 hygromycin. Although early globular embryos were observed up to 15 mg L^-1, further embryo development was inhibited at 10 mg L^-1. Interestingly, cefotaxime (250 and 500 mg L^-1) promoted a marked effect on enhancing fresh weight of calli, accompanied by decrease in embryo regeneration, whereas timentin concentrations (150 and 300 mg L^-1) did not affect embryo differentiation as compared to the control treatment.

• Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures

  15-23.

  E. A. de T. Picoli

  S. H. Brommonschenkel

  P. R. Cecon

  D. J. H. Da Silva

  M. G. Fári

  W. C. Otoni

  Views:

  407

  The effects of kanamycin and hygromycin-based selection and co-culture temperature ranging from 22 to 28 °C upon eggplant transformation efficiency were evaluated. Both morphogenic pathways, somatic embryogenesis and organogenesis, were adopted using cotiledonary and hypocotyl explants, respectively. Somatic embryos were recovered in the presence of both antibiotics, although lesser escapes were observed in hygromycin-supplemented medium. Indeed, selection provided by this antibiotic was more efficient compared to kanamycin, nevertheless, shoot regeneration was not observed with hygromycin. Significant difference on the frequency of cotiledonary explants displaying callus (FEC) was observed as embryogenesis was concerned, although a higher number of embryos was observed in hygromycin selective media. The frequency of explants presenting callus (FEC), embryos (FEE) and shoots or buds (FERG) did not differ statistically for the tested co-culture temperatures, although higher regenerant number was observed at 24 °C.

• Rate of scab infection and quality parameters of apple fruit in organic and integrated production systems

  63-67.

  I. Gonda

  I. J. Holb

  K. Bitskey

  Views:

  776

  In this study, the rate of scab infection and quality parameters (content of vitamin-C, dry matter, sugar, total acids, specific weight, quality index and flesh firmness) of some apple cultivars are given, moreover, the relation between those characters was examined in two environmental systems (organic and integrated) in a rainy season. In the organic fruit production, scab infection of leaves was significantly higher than in the integrated system, in case of the same cultivars, which means that the rate of decrease in leaf area was heavier in the organic production. Cultivars did not differ statistically in parameters of inner content when the integrated system was compared to the organic one. Results did not show statistical difference between loss of leaf area caused by apple scab and the parameters of inner content, which is attributed to the regeneration of leaf area due to favourable weather conditions. Data on apple scab and inner content of the fruit justify essentially the environmental systems of apple production.

   

• Summer pruning of sweet cherry trees and an inquiry of winter frost damages

  41-44.

  B. Vaszily

  I. Gonda

  M. Soltész

  Views:

  9922

  One of the most demanded research projects is the intensification of fruit production. The use of dwarfing stocks is a moderate solution as their effect is scarcely satisfactory. Climatic conditions of Hungary are continental in Eastern Europe, where Atlantic and Mediterranean effects are interacting with the continentals in a kind of basin with characters of its own. Capricious meteorological episodes are often disturbing the security of development and fruiting of trees:
  • winter frosts are damaging the cambium and fruiting structures of trees
  • late spring frosts destroy cambium and flowers
  • early autumn frosts hurt the leaves
  • excessive precipitation impairs the growing fruits
  • drought periods during the summer caused water stress disturbing water husbandry.

  Vigorous stocks still prevail in the practice, and they ought to withstand challenges of weather hazards. The strong vigour of plants delaysthe process of senescence and the tendency of getting bald, and regeneration of plants is a sign of vitality. In present research, the trees have been trained on vigorous Prunus mahaleb stocks. Summer pruning was one of the important tools of intensive growing techniques. They were compared with traditional techniques and with plastic foil protected trees observing the vegetative as well as generative growth of them.