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• Genetic transformation of bean callus via Agrobacterium- mediated DNA transfer

  49-53.

  E. Eissa Ahmed

  Views:

  210

  Callus cultures were induced from hypocotyl of young bean seedlings. Callus developed and maintenaned on B5 medium supplemented with 2mg/1 2,4-D and 1 mg/1 kinetin. The results demonstrate that A. tumefacins-mediated transformation is a convenient method to obtain transient gene expression in callus of bean. The results have shown that the bean callus co-cultivated with A. tumefaciens can be transformed to get heibicide Finale (glufosinate-ammonium) resistant GUS positive tissues. Southern blot analysis of transformed calli showed integration of gusA marker gene carried by a binary vector. Transformed calli were selected on herbicide containing media. Data of molecular analysis (Southern blotting) confirmed the insertion of gusA gene in the genome of herbicide resistant calli with bar gene. There are three evidences that calli are stable transformants: (1) herbicide resistance, (2) GUS activity which is indicative since the coding region containing an intron, (3) the results of Southern hybridization technique.

• Evaluation of some Achillea L. accessions based on morphological, cytological and chemical characteristics

  69-74.

  K. Inotai

  É. Németh

  I. Pintér

  K. Jager

  Sz. Sárosi

  Views:

  278

  Taxonomical evaluation of six taxa of the Achillea aggregate was carried out to determine their potential for breeding and cultivation. We used complex morphological, cytological and chemical aspects of characterisation. Three taxa of tetraploid genom and high chamazulene contents (33-40% ess.oil) proved to be A. collina Becker. A strain of wild origin had also high -bisabolol content and high oil level, comparable with the selected cultivars. A population was identified as new chemotype of A. pannonica Scheele with typical morphological and cytological traits, a wide sesquiterpene spectrum but lacking of 1.8 cineole and -pinene. We found a mixed population which consisted of both A. collina and A. pannonica plants. A tetraploid, azuleneless taxon could be concluded as A. pratensis Saukel and Langer not described before in Hungary. The diverse performance of the populations calls the attention to the significance of controlled plant raw in phytopharmaceutical products.

• Self-incompatibility in plums (Prunus salicina Lindl., Prunus cerasifera Ehrh. and Prunus domestica L.). A minireview

  137-140.

  A. Hegedűs

  J. Halász

  Views:

  695

  Japanese plums (P salicina) and cherry plums (P cerasifera) are diploid species, while European plum (P. domestica) cultivars are hexaploids. Most diploid species are self-incompatible while fertility relations of the hexaploid European plums are variable between self-incompatibility and self-compatibility. About twenty S-alleles and six inter-incompatibility groups and one S-haplotype responsible for the self-fruitful phenotype were described in Japanese plum cultivars, but studies on cherry plums and even on the European plum cultivars are severely restricted. This review is focused on the available information obtained from myrobalans and European plums; and discusses recent hypotheses regarding the putative origin of the hexaploid plums, and thereby indicates the possibility of allele flow between different plum species.