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Molecular analysis of strawberry cultivars using RAPD, AP-PCR and STS markers
24-28.Views:127Seventeen strawberry (Fragaria x ananassa Duch.) cultivars representing the national list of Hungary, were subjected to RAPD, AP—PCR and STS analysis. Of the 31 decamer and oligomer primers tested 26 primers produced polymorphic patterns. 45 polymorphic fragments were analysed, ranging between 200-2800 by in size. Based on the data, similarity coefficients (Jaccard index and Simple matching coefficient) were calculated, and dendrograms were constructed using the unweighted pair group method of arithmetic averages (UPGMA). The dendrograms only partly reflect the known pedigree data. Specific RAPD markers were identified for cultivars F5, Pocahontas and Rabunda.
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Floral biological investigations of apple cultivars in relation to fire blight
9-14.Views:163Floral activity was studied in two apple cultivars: an Erwinia-tolerant (Treedorn') and a sensitive one (`Sampion'). Since more types of protogyny occur in apples, the period of stigma activity is different. Papillae of the exposed stigma in flowers of 'Freedom' function longer (usually more than a week) than in the delayed homogamous `Sampion'. Despite of this, cv. 'Freedom' is tolerant to Erwinia amylovora (Burr.) Winslow et al., suggesting no relationship between the floral biological type (including the exposure and longevity of stigma) and the infection by E. amylovora. According to SEM micrographs, nectary stomata in `Freedom' are already open in the flower bud, where nectar secretion starts and continues until the senescence of the stigma. However, the long period of nectar secretion does not create optimal conditions for bacterial growth, since nectar production is scant in the flowers of 'Freedom'. The surface of the nectary, its nectar-retaining capacity, and the amount and concentration of nectar may influence the susceptibility of apple cultivars. It is manifested well by the smooth nectary surface with nectary stomata rising slightly above the epidermis in flowers of cv. 'Freedom', contrasting the wrinkled, striate nectary surface with slightly sunken stomata in the flowers of 'Sampion'.
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Security of growing habits and bud formation of German sour cherry varieties
45-48.Views:229Intensive sour cherry production is concerned to find the most productive varieties under special growing conditions. High planting density, adapted to manual picking or on the other hand to mechanical harvesting. Almost as important is the prolongation of the ripening season by enlarging the choice of varieties. Unfortunately, three of our four leading commercial varieties are ripe almost at the same date.
Therefore, new varieties ought to be examined thoroughly. In the Pallag Experimental Station of the Debrecen University, five European varieties have been grown (’Schattenmorelle’, ’PI-SA 12,100’, ’Jade’, ’Gerema’, ’Achat’) and a Hungarian one, ’Debreceni bôtermô’ used as a check for the experiments to compare their growing and yielding habits in 2010. The plantation was three years old, standing on Prunus mahaleb rootstocks, in high density (5 x 2 m) and trained to slender spindle crowns. The results are proving that some of the varieties in
question are suitable to prolong the harvesting season. Growing habits and yields of the varieties related to the variety ’Debreceni bôtermô’ were similar or even better as ’Jade’, ’Gerema’ and ’Achat’. The ’Jade’ excelled with its vitality and ’Gerema’ with its generative character. Further studies are expected to prove the utility of
one of them at least to enlarge the ripening season on the fruit market. -
Efficacy of N-phenylphtalamic acid in some Solanaceae species
89-91.Views:117: N-phenylphthalamic acid — Cl4H1 1 NO3 (Nevirol 60 WP) was successfully used for enhancing yield in some important vegetable crops namely, tomato (Lycopersicon esculentum), chilli (Capsicum annuum) and brinjal (Solanum melongena) of Solanaceae. Aqueous sprays with 0.2% and 3.0% significantly enhanced fruit production in chilli and tomato respectively. On the other hand, various treatments in brinjal failed to enhance yield significantly. The increase in yield in both tomato and chilli is largely due to increase in the number of flowers and fruit-set percentage.
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Growing characteristics of apple cultivars in environmentally friendly growing systems
7-10.Views:244Nowadays the condition of the good saleability of the fruit is the application of controlled environmentally friendly technologies. Success of the growing is basically influenced by the production value of the cultivars, and their flexibility to the different technologies. In our examinations the effect of the integrated and organic farming system has been evaluated on the growing characteristics of the apple cultivars. According to our results the trunk thickness of the trees both in the initial and both in the later bearing period is higher in the integratedproduction compared to the organic farming system. This differences most likely caused by the variance of the crop load.
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Relation of sour cherry blooming dynamics and meteorological variables
17-23.Views:250The aim of our research was to identify the role of weather parameters in the development of the start date and length of blooming. In order to achieve this, we examined how meteorological conditions of a particular year influence the start date and length of blooming in different years (dry, wet, cool, hot, sunny, cloudy). The meteorological parameters were the following: maximum temperature, minimum temperature, average temperature, precipitation, length of sunlight, difference of daytime and nightime temperatures, potential evaporation-PET, Huglin-index,Winkler-index, climatic water balance which can be calculated as a difference of precipitation and potential evaporation. In this study we wanted to find out whether early start of blooming results in a longer blooming period or if there is a generally faster blooming period when blooming starts later. Based on the results we can say that early start of blooming resulted in extended blooming period for three sour cherry varieties at the examined production sites. The start of blooming showed the closest relation with the difference of average daytime and nightime temperatures of the 30-day period prior to blooming. Significant relation was also detected with the thermic indexes as well as with maximum temperatures, climatic water balance and the degree of potential evaporation.We examined how weather 30 days prior and during blooming influenced the length of phases. Results showed that precipitation prior and during blooming is in significant relation with the length of blooming. We detected significant relation between daytime and nightime temperature differences and the length of blooming. The nature of the relation indicates that blooming periods were shorter in case of increasing temperature differences.We found that shorter blooming lengths occurred when maximum temperatures averaged between 13.5–14.5 ºC 30 days prior to blooming when examining the relation between blooming length and maximum temperature. If the average of maximum temperatures was below 13 ºC or exceeded 15 ºC during this period, then we could calculate with a blooming period longer than ten days. We proved that little precipitation and high temperature accelerates physiological processes, therefore we could calculate with an accelerated blooming and shorter blooming period.
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Effects of different pollination treatments in genotypes of Prunus salicina Lindl.
141-146.Views:123The low productivity in the Japanese plum (Prunus salicina Lindl) is related with self-incompatibility characteristics, so other species or varieties that act as pollinators need to be present to improve fruit production. The objective of this work was to study the efficiency of pollination in different genotypes of P. salicina using treatments of natural self-pollination, cross-pollination with P. armeniaca cv. Giada and open pollination. These treatments were evaluated through viability techniques and in vitro and in vivo germination of pollen grains; the growth of pollen tubes along the pistil was also observed. Genotypes used in this study showed differences for each one of the pollination treatments. Some genotypes showed signs of self-sterility and interincompatibility with P. armeniaca cv. Giada, while others showed partial self-fertility characteristics or pseudocompatibility. Moreover, some genotypes showed a higher affinity coefficient with cv. Giada and these will be indicating a possible intercompatibility. These studies will be an important contribution breeding and selection of intra and intercompatible genotypes to be used in commercial orchards.
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Organic versus integrated apple growing: I. differences in soil and leaf parameters
57-60.Views:208The aim our study was to establish whether significant differences in nutrients uptake and quality of soil and leaf exist between organic and integrated grown apples. The study was performed at the orchard Fruit Research Station, University of Debrecen, at Debrecen-Pallag during 2002–2004. Macro and micro elements were measured in soil and plant samples. Analyses of variance of soil nitrogen data indicated highly significant differences between the two management systems (P < 0.001) for each examined nitrogen fraction. Analyses of variance of soil phosphate data indicated significant differences (P < 0.05) between the two management systems for orto-PO4 3– contents. Our data indicated that highly significant differences between the two management systems (P < 0.001) for magnesium, copper, and zinc; while significant differences between the two management systems was at P = 0.007 for calcium. Three year’s data of leaf phosphorus, sulphur and zinc were not shown significant differences between production systems. Nevertheless manganese and copper contents of leaves were higher in the organic orchard compared to the integrated one.