The aim of this study was to test the use of commercially available arbuscular mycorrhiza (AM) inoculant Symbivit, a mixture of six species of Glomus spp., in spice pepper field cultivation. The inoculants containing arbuscular mycorrhizal fungi (AMF) was able to establish a symbiosis in the rhizosphere of pepper plants and mycorrhizal inoculation increased fresh and dry weights of shoots of spice pepper cv. Szegedi and only fresh weight of Kalocsai type. There were no significant differences in the root weights due to treatment only in fresh weight of Kalocsai pepper type. Treated plants of both variants exhibited an increase in cumulative crop production compared with control non-treated plants and the growth response of pepper was higher for var. Szegedi than var. Kalocsai. Mycorrhizal inoculation had a great positive effect on external hyphal length of AMF also showing differences in that between Kalocsai and Szegedi variants. The root colonization showed seasonality by treated and non-treated plants. The lowest degree of colonization was observed in June in general and colonization percent increased during vegetative development and there was a slight decrease at harvesting. In conclusion, it can be stated that inoculation with Symbivit containing mycorrhizal fungi could be an integral part of spice pepper production.