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A simple but powerful tool for the assessment of financial progress in EU co-funded grant programmes – Simple Progress Overview Tool (SPOT)
67-72Views:116Mid-term evaluation of grant programmes always aim to assess the financial progress of the programme. Progress to date assessment is conducted via addressing three issues: by overviewing progress so far, by exploring the reasons behind the current level of progress, and by answering the specific evaluation questions formulated as a result of progress overview. The Simple Progress Overview Tool is a method and a tool facilitating the overview of financial progress, supporting the exploration of reasons behind the current level of progress and enabling the identification of targeted further evaluation questions. The methodology applies the Du Pont approach of division to factors, in which the variable describing a phenomenon is divided into the multiplication of factors (milestone stages of the process) influencing the variable. Also, this division to factors facilitates the identification of reasons behind progress. The factors carry individually meaningful information to the evaluation of progress. This method was used by many EU member states including the Czech Republic, Romania, and Malta for the evaluation of progress in the course of the mid-term evaluation of their National Strategic Reference Frameworks.
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Using of e-learning in agricultural training programs
117-124Views:62We use the Moodle at the University of Debrecen, Businessand Agricultural Department since January 2007. The Moodle is an open source Learning Management System. Learning Management System (or LMS) which is a software package, which enables the management and delivery of learning content and resources to students. Most LMS systems are web-based to facilitate „anytime, anywhere” access to learning content and administration. LMS tracks student progress in a course and indicates completions. At the least, learning management systems
track individual student progress, record scores of quizzes and tests within an online learning program, and track course completions. The Moodle has more and more function at our Department in education. In January we worked to introduce Moodle in our Faculty. Therefore, we took lessons for the tutors about the usage of the Moodle. Our aim was to develop such a learning system, which is an integral part of educational process,
to improve the efficiency of agricultural higher education. -
Gene resources for improvement of drought tolerance and yield quality in dry pea breeding
105-111Views:86The effectiveness of selection for improved drought tolerance and consumption quality in the progeny of crosses between pea cultivars with semi-leafless (afila) and normal leaves and different origins, respectively, were investigated. After single crosses, parent cultivars and F1, F2 and F3 generations were grown under non-irrigated conditions in the same trials. We created a colour scale from 1 to 9 to measure statistically the shade of seed colour. The tolerance of genotypes against high temperature was measured by the number of pods per plant. The 3:1 segregation
observed in the F2 generation of crosses between semi-leafless and conventional cultivars indicated that the semi-leafless character is determined by a recessive gene. In contrast, the ratios of conventional (Af) and semi-leafless (af) genotypes were 7:1 and 9:1 ratio in the progenies of crosses of Af × af. The genetic progress was effective for improving the seed quality in F3 generation from crosses Af x af where we found that multiple
dominant alleles controlled the orange colour of cotyledons and its high heritability (h2 A=0,63). Selection is more effective in producing the genotypes with high yield and normal leaves if the crosses were made between the western European cultivars such as semi-leafless Profi and Delta used as maternal cultivars and conventional Auralia cultivars. In this case, there were decreases in the consumption quality, such as seed size and shade of colour. The selection based on the seed weight of single plants for increasing drought tolerance seemed to be more effective in F4
strains with normal leaves originated from Czechoslovakian maternal cultivar Y228; however, the genetic progress in the improvement of seed size and colour quality was slow. -
A practical means of setting the scene for evaluating aggregated absorption risk in EU co-funded programmes related to project failure
63-66Views:106The main purpose of the mid-term evaluation of grant programmes is to assess relevance, progress, effectiveness and efficiency. There are several methodologies, tools and techniques applied in various evaluations to assess these programme aspects. An important area of the evaluation of both the financial progress and the effectiveness of the programme is to evaluate the risk of absorption which is indicated by various factors, inter alia risk of supported project failure. If a project fails in any EU co-funded programme in the stage of preparation or implementation, then the fund already committed to that particular project (assumed to have already been absorbed or “spent”) is to be reused again or is to face decommitment (funds have to be paid back to the EU). There are strict EU regulations governing the time scale of this re-use (n+2, n+3 rules), therefore it is of major importance to assess the risk severity (measured as the resultant of the volume of grant at risk and the chance of project failure) and build up an early warning mechanism which indicates if the risk reaches a critical level that requires immediate intervention.
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Practical experiences in Moodle LMS
129-133Views:59We use Moodle at the University of Debrecen, Business- and Agricultural Department since January 2007. Moodle is an open source Learning Management System.
Learning Management System (or LMS) is a software package that enables the management and delivery of learning content and resources to students. Most LMS systems are web-based to facilitate "anytime, anywhere" access to learning content and administration. LMS tracks student progress in a course and indicates completions. At the least, learning management systems track individual student progress, record scores of quizzes and tests within an online learning program, and track course completions.
Moodle has more and more function at our Department in education. At present, we work to introduce Moodle in our Faculty. Therefore, we took lessons for the tutors about the usage of the Moodle. Our aim to develop such a learning system, which is an integral part of educational process. -
Effect of the claw disorders on production performance in dairy cattle
15-19Views:158The aim of the study was the influence of claw disorders on production traits in dairy cattle. Observed were claw traits as claw angle, claw length, heel index, claw height, claw diagonal and claw width. Right hind claw after functional trimming was evaluated. Occurrence of claw disorders like interdigital dermatitis and heel erosion (IDHE), digital dermatitis (DD) and sole ulcer (SV) as well as the progress of disease were observed. Basic summary and variation statistics was performed by the SAS software. Two herds were included in the study. Holstein dairy cows (n=101) produced 11 875 kg of milk, 468 kg (3.94%) of fat and 396 kg (3.34%) of proteins. Holstein cows were affected with IDHE in 22 cases, with DD in 6 cases and with sole ulcer in 10 cases. Slovak Simmental dairy cows (n=101) produced 5834 kg of milk, 258 kg (4.44%) of fat and 209 kg (3.59%) of proteins. Slovak Simmental cows were affected with IDHE in 8 cases, with DD in 5 cases and with sole ulcer in 19 cases. Observed was that claw disorders caused deviations of milk production.
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Will there be a horticultural triangle (cluster)? Thoughts about the reconstruction of the Hungarian horticulture between two European regions
21-27Views:99The authors of this study seek the answer to the question how to develop, in the first decade or decades of the 21st century, the university-level
horticultural scientific training, the horticultural innovation and the scientific co-operation between companies and universities in Debrecen and
in the North Great Plain Region and – in a wider sense – in Hungary to a standard being competitive even in European terms. With the synthesis
of the prospects of past, present and future, they drew the following conclusions. The reconstruction of agriculture, horticulture and food industry
is a part of reforming Hungary's countryside. Horticulture, producing high added value, will be able to decisively contribute to the plan whereas
the value presently produced in an agriculturally cultivated area of 1,000 euros/hectare can reach 2,000 to 3,000 euros in the next two decades.
A necessary and indispensable precondition to achieving this is the strengthening of the innovation output of the Hungarian horticultural sector.
Despite the numerous technical criticisms formulated in connection with the serious problems of Hungarian agricultural and horticultural
scientific innovation, no progress has been made in this field for the past one and a half decade. The scientific research of this topic hardly
continued or did not continue at all, the up-to-date surveys and in-depth analyses were missing. The objective, basic principles and tasks of the
Act CXXXIV of 2004 (TTI) enacted concerning research-development and technological innovation are clear and progressive. The co-operation
between the National Research Technology Office and the Hungarian Academy of Sciences, the setting up of the Innovation Fund are heartening
opportunities. These – along with the new Higher Education Bill to be passed – may as well be suitable for restarting the Hungarian agricultural
and horticultural scientific innovation. In our opinion, this requires a new, well-considered national agricultural programme, which can be
conceived in the framework of the "Ferenc Entz National Horticultural Plan" proposed by us for horticulture. In the most eastern Hungarian
university knowledge centre, at the University of Debrecen, the continuing of the horticultural scientific innovation strategy started in the last
decade may be the focal point and generator of the development of the so-called "Hungarian Horticultural Triangle”, or "Hungarian
Horticultural Cluster". This region comprises the Northern and Southern Great Plain Regions and the area between the Danube and Tisza
Rivers. Here, about 70 to 75% of the total Hungarian horticultural commodity stock is produced. The objective of the HORT-INNOTECH
DEBRECEN programme planned in 2004 by the University of Debrecen, Centre of Agricultural Science is to establish the horticultural scientific
research-development and technological innovation structure and knowledge base of the Hungarian Horticultural Triangle / Hungarian
Horticultural Cluster. In harmony with this, the objectives are to bring about competitive, new horticultural products, to improve the conditions
of utilising them, to enhance the competitiveness of enterprises based on technological innovation, to make use of the research-development and
innovation opportunities available in the regions in an efficient manner, to as full extent as possible, to encourage the creation of places of
employment producing high added value in the field of horticulture, to improve the technical skills of those employed in horticultural researchdevelopment and to promote their enhanced recognition by the society. -
History of origin and development of replacement of plants crop rotations is in world agriculture
53-56Views:118In practice of world agriculture a long ago the known problem of decline of harvests of agricultural cultures at their permanent growing, scientific explanation of this phenomenon became possible only with appearance of natural sciences. At first the declines of harvests bound to the toxic action of root excretions cultures on her repeated sowing, with development of humus theory of feed of plants of diminishing of harvests at the permanent sowing began to explain impoverishment of soil on a humus.
During a few centuries the known farmers development the looks in relation to forming of scientific bases of construction of replacement of plants crop rotations in the world systems of agriculture, set history of their development and improvement. The analysis of influence of possibility of optimal satiation of replacement of plants crop rotations is conducted by agricultural cultures on the level of fertility of soil, water and nourishing modes and their productivity. For the terms of the insufficient moistening a positive action is marked black pair on the improvement of the water mode of soil in crop rotations.
In historiography the problem of introduction and mastering of replacement of plants crop rotations for the decision of scientific and practical tasks of agricultural production is represented in many-sided aspects, worked out and the recommended replacement of plants crop rotations that are base on zonal principle of development of world agriculture that passed the protracted term of test and counted on various specialization of economies. But for today development of scientific and technical progress requires intensification of agricultural production with the use of intensive crop rotations and growing of high-performance cultures.
Hereupon there was a necessity of realization of analysis of the systems of historical value of scientifically-practical knowledge about development and improvement of replacement of plants crop rotations, as it gives an opportunity to work out to recommending a production with the use of the most effective elements of the past on modern agrarian business and allows to forecast them on the future.
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Some strategic aspects of animal protein production
11-19Views:178The access to food shaped human societies and dietary models throughout the history of mankind. Animal protein consumption became a part of human culture. Data are presented showing the relationship of daily calorie and animal protein consumption as affected by capita GDP changes. Examples are presented how genetic improvement of animal and fodder plants influenced the resource efficiency and the overall environmental footprint per unit product. The two examples presented are: the dairy industry of the USA the 1944 and 2007 situation, and the Hungarian broiler chicken sector considering data relevant to 1930, 1960 and 2010. In both cases, dramatic improvements in resource efficiency could be demonstrated. The agricultural area required to animal feed production was reduced by more than 80% in both cases per unit product. Future possibilities are briefly discussed, referring to the still unutilized land reserves of the Globe, the new evolving technologies in progress inclusive the CASPR/Cas 9 genetic editing methods.
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The development of the scientific bases of horticulture and the history of horticultural innovation in the Northern Great Plain region and in Debrecen
17-20Views:75It can be told about the second half of the XXth century that, apart from preferring the large-scale growing of field plants and the largescale
livestock farming, corresponding to the central political will of the communist era, the significance and innovation output of
horticultural education in Debrecen was rather of follow-up trait, of secondary importance. The Tobacco Research Institute continued the
research work, and then even this activity was stopped. According to a survey finished in 1997, the mentioned institute had no invention,
granted patent, protection for registered model or any application for patent in progress at the Hungarian Patent Office. Until this time, invention activity at the University of Agricultural Sciences was of medium standard. In the National Patent Office, seven patent applications related to agricultural production and nine patent applications for other fields submitted under the inventors’ names were recorded. In the same period, the Cereal Research Institute (Szeged) led the absolute innovation list of Hungarian agricultural R+D institutions and university education institutes by submitting 164 own patent applications. Both in domestic and international terms, the horticultural innovation conducted at the University of Agricultural Sciences, despite the individual research results and achievements deserving recognition, without appropriate background – remained unnoticed. Let us put it this way: for the past decades, the light of the Debrecen Flower Carnival has not been thrown on the horticultural teachers and researchers of Debrecen. -
Biofuel regulations in the USA and the EU
25-31Views:113Climate change, the increasing dependency on crude oil and other fossile fuels, the expanding import and the increasing energy costs make the world’s societies and its economies vulnerable.
The development of effective renewable energy resources has been a priority for the US since the first oil embargo. While there was only little progress for around two decades, biofuel use and production has been showing a rapidly increasing tendency since the mid 90’s.
The EU’s objective of renewable energy resources is one of the main priorities of the Europe 2020 strategy. The European Union aims to increase the share of energy produced from renewable resources in the whole energy supply and by 10% in the transport-related energy supply by 20% until 2020.
The renewable energy sector is the only one which is able to reduce GHG emission and pollution while utilising local energy resources and stimulating cutting edge industrial sectors; therefore, it is indispensable to develop a proper framework and regulation for supporting renewable energies.
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Genetic progress in winter wheat quality and quantity parameters
71-75Views:137Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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Changes in weed flora of basket willow (Salix viminalis L.) under different soil nutrient supply
116-120Views:129The world is in a continuous progress, as a result of which energy consumption and with this the release of gases with adverse impact show rapid increase. As a result of the survey conducted by the International Energy Agency, if the major economic powers do not initiate a change in their energy policy, the increase of energy consumption may as well reach 40 % by 2030. This increased energy demand is getting more and more difficult to fulfill with the fossil energy resources, which is to lead to an increasing significance of renewable energy resources. In Hungary, these energy resources are the best to provide with biomass growth. Biomass growth for energetic purpose can mostly be provided by energy plants, out of which “energy willow” (Salix viminalis L.) is outstanding with its high yield and with its excellent burning technology characteristics of its timber. The willow’s cropping technology is being established in our country. One of our tasks is to work out an adequate weed control plan. The professional and safe use of herbicides can increase the success of production. In our paper, we discuss the weed flora data collected on treatments applied in the different fertilizer and compost. We started our survey in 2010. We examined twelve different fertilizer and compost treated areas. The dominant weeds were: Amaranthus retroflexus, Chenopodium album, Echinochloa crus-galli among annuals; Cirsium arvense and Agropyron repens among the perennials.
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The importance of millet production in regional production, with special emphasis on climate change
141-146Views:137Regional production is a traditional production structure developed adjusting to the geographical, climatic, biological and soil conditions in given production regions, a certain territorial specification of agricultural production, and a type of farming that best fits the natural conditions and takes the biological needs of plant and animal species into account as fully as possible. The most probable element of risk in plant production is the changeable, extreme weather. That is the reason why the specific characteristics of the place of production and the characteristics of regional production should be considered to a greater extent. The establishment of the range of varieties appropriate for the place of production is the key issue in regional production. One of our historically grown cereal plants that perfectly fits regional production is millet. Due to its short growing season, favourable reproduction ratio and the fact that it is relatively undemanding, it used to be grown in larger quantities in the middle ages. Its good nutritional values made it an important food item, but over time, as a result of industrialisation and technological progress; it has been eclipsed by other cereal crops. In our country it is mainly used to cook porridge, but it is also used in the form of flour and as a base material in the spirit drinks sector. In the recent decades, millet has been applied only in a small area, mostly as a secondary crop in areas that dried out from drainage water in late spring, or as a replacement of extinct sowings due to its late sowing time. Water will be the most significant factor for the future of agriculture, especially considering climate change.My examinations took place in the area of the Institutes for Agricultural Research and Educational Farm of University of Debrecen, in the Research Institute of Nyíregyháza, in a small-plot experiment with four replications in 2016. -
Open Method of Coordination in the European research policy
182-187Views:69Research, development and innovation (R+D) are of great importance nowadays as regards the competetiveness and economic growth of any given country. In the European Union the Lisbon Strategy considers R+D as a crutial means to enhance european competetiveness. The EU is substansially lagging behind its main competitors (USA, Japan) in this domain, furthermore developing countries (China, India) have recently been making
great progress, thus posing additional challanges to our continent. The EU needs to assess the present situation as soon as possible and meet the latest challenges in the most efficient way. In order to achieve this objective, the concept of the European Research Area (ERA) has been elaborated with the aim of reorganising, regrouping and integrating existing structures. The open method of coordination (OMC) is playing an important role in the implementation of this common area. It constitutes an alternative policy-making merthod to pure „intergovernmentalism” and the
„community method”. The OMC in the field of research policy is coordinated by the Scientific and Research Committe (CREST), which provides a forum for member states to share their experience and reach their common goals. -
Experiments for Isolating and Culturing Soil-borne Mycobacteria (Contemporary Publication)
107-110Views:58On grounds of the several thousand tests performed in the field of this topic, the following conclusions may be arrived at:
1. The informations available and the experimental data on soil mycobacteria are very incomplete.
2. Of the 77 strains isolated from similar soil types so far, and adaptable for pure basic culture, 47 strains are confusingly similar, from morphological aspects, to the mycobacteria isolated from clinical material.
3. The apparently homogeneous cultures isolated from the soil are generally co-infected and, therefore, the morphological, biochemical, and other physiological characteristics of the isolated strains can be studied only on base cultures after purification.
4. For the isolation of the soil mycobacteria experiments qualified hitherto as most suitable processes the 4 or 1 per cent NaOH neutralized with H2SO4, and the 1 per cent NaOH or 1 per cent Na3PO4 treatments, on Gottsacker agar medium with plate or top pouring, at a temperature of 29 to 37 C°, in a soil suspension sequence of 1:500 to 1:5000 final dilution.
5. The Ziehl-Nielsen staining of the isolated mycobacteria composed to sub-cultures is best performed by heating with an infra red radiator from above, instead of the gas flame used so far to heat from below.
The repetition of the biochemical test of the hitherto isolated 77 purified strains is under progress, and will be reported on in our next scientific publication. -
Comparison of supplier quality assurance methods for compound feed manufacturing from efficiency, product consistency and economical point of view
29-34Views:87Feed manufacturing and human food production are the main routes of use for agricultural products. The food industryparticularly has intensively implemented the recent quality management principles and developed systems that facilitate the continuous improvement and efficiency of the industrial production. Feed production has taken similar approaches however the intensity of deployment at the manufacturer and the rollout towards its supply chain has shown slower progress. The methods, that the feed manufacturer manages the supplier chain of mainly primer agricultural products according to, have a certain impact on the efficiency of the inbound operations, feed product quality and its consistency as well as on other resources. These methods have been built on sound quality management principles that are stated not only in quality standards but also in relevant regulations. Current study addresses the questions related to the link between supply chain quality management and feed product. The objective of the frontier research was to highlight the theoretical possibilities and benefits of the robust design methodimplemented into animal feed manufacturing dealing with highly variable ingredients.
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Stevia (Stevia rebaudiana B.) yield in the case of different production technologies
71-77Views:185Stevia rebaudiana B. offers a natural alternative of sweetening, potential health promotion plant, and our country shows increased interest about cultivation in Hungary in addition to the neighboring European countries. The agricultural production system installation necessary
understanding of the needs of the plant, as well as exploring the agronomic potential. Field experiments were conducted in 2015 with the aim to highlight some of the technological production parameters and correlations between them. We have reviewed the most important agronomic factors, the spacing (50×50 cm and 33×33 cm), ground covering (agro-cloth covered and uncovered/ control), their vegetative growth (first-,second-order branch), herb yield and quality in effect.
Based on empirical evidence, that the Hungarian climates also have the opportunity of 3 cuttings during a growing season, besides of early planting, optimal climatic conditions (in October didn’t reduce the daily minimum temperature below 0 °C, after cuttings the maximum interval values remain below 40 °C), and adequate water supply and crop protection facility. The 33×33 cm spacing evaporates more than 50×50 cm spacing, because of the dense population, the continuous canopy less able to breathe, so there are serious chances to the pathogen colonization.
Based on the results of our research to the wider 50×50 cm spacing favorable appreciate the stevia optimal progress in terms of qualitative and quantitative parameters of the yield. The stevia yields produced in the Northern Great Plains field cultivation can produce similar results as stevia crop yields in warmer climates, where the primary crops. Our research experience suggest that there is a viable domestic stevia cultivation, developing the necessary technology is still growing further investigation justifies. -
Investigation of the Quality of Winter Wheat in a Sulphur Fertilisation Experiment, 2001/2002
153-156Views:103We have started a small parcell and a factorial S fertilization experiment with winter wheat in the 2001/2002 cropping year to examine its effect on yield and quality. The scene of experiment was the Latokep Experimental Station of the DE ATC (calcareous chernozeem) in case of small parcell examination and the Agricultural Company of Felsőzsolca (brown forest soil) in case od factorial examination.
The protein and gluten content of the grain was investigated with PerCon Inframatic 9001 NIR Analyser, then we have measured these parameters with PerCon 8620 infra appliance. After the milling we measured the following parameters: glutenindex, farinographic parameters (farinographic index, water absorption capacity, dough development time, stability, softening, extension) and valorigraphic index.
Based on the results there’s no justified relation between the fertilization and the protein and gluten content. The valorigraphic index of the samples taken from Felsőzsolca factory characteristically increased as a result of the S-fertilization. In the small-parcell experiment the values of the water absorption capacity, the dough development time and the softening parameters from the valorigrphic parameters depended significantly from the mineral treatments. Signifikant quality improvement wasn’t experieced.
There was sampling in all of critical phenophase (…). The green plant samples were examined on element content with ICP-OES. These measurements are currently in progress. -
Information and Communication Technologies in tourism
49-53Views:144Technological progress and tourism have gone hand in hand for years. Information communication technology (ICT) and tourism are two of the most dynamic motivators of the emerging global economy. Tourism can be considered as one of the most profitable sectors of the Hungarian economy, and in rural areas it is often the only successful economic activity.
Development of ICTs and the expansion of the Internet have changed dramatically in the past few decades. This process is noticeable in
Hungary, as well. Platform of tourism increasingly get to the Internet nowadays, which is vitally important because tourism is an information-intense industry. Therefore, it is critical to understand changes in technologies in order to maintain the crucial role of this sector in the Hungarian economy.
The aim of my study is to support the significance of tourism in Hungary and especially in the North Plain Region with the help of statistical data. Then, I try to show how ICTs appear in this sector and emphasize the role of these tools with some concrete examples. -
The specification of the growing temperature on PDA of chestnut blight pathogen (Cryphonectria parasitica (Murr.) Barr) to optimize timing for biocontrol treatments by hypovirulent strains
29-32Views:161The most destructive pathogen for the European chestnut trees is the blight fungus Cryphonectria parasitica (Murr.) Barr. The biological control is the only effective possibility to apply in situ biocontrol by hypovirulent strains against compatible virulent (wild) fungus strains. The infested bark tissues can inoculate by drilling holes surrounding and putting into agar discs interwoven by the appropriate vegetative compatible group (VCG) hypovirulent fungus strains. This latest can pass those virus-like particles (VLPs) by parasexual contact (called hypha-anastomosis)which responsible to hypovirulence. A laboratory experiment was made to find the optimal times to carry out the treatments. The intensity of growth of fungal colonies were analysed on different temperatures. The growth of the fungus on low temperature were rather slow,according to our expectations. On higher temperature the colony progress were the same as on the optimal 20–25 °C. These observations and the environment determine the date of the field applications under Hungarian weather conditions. It means the optimal treatment periods can be May or end of September to middle October in Hungary.
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Application of microsatellite fingerprints for pedigree analysis of Hungaricum grapevine varieties
71-77Views:99the Carpathian Basin were involved into our examination, which aimed at genotyping their accessions. DNA fingerprints of 101 varieties were determined with 6 microsatellite markers till 2005, resulting in successful discrimination of the accessions. Based on these results for pedigree determination, even more cultivars and primers were involved into the analyses. For studying the origin of Csabagyöngye and for proving the parent-progeny relations of Irsai Olivér and Mátrai muskotály, 19 microsatellite markers were applied, while 11 were selected for tracing the origin of Királyleányka. Genetic distances between the varieties were estimated with cluster analysis and demonstrated by dendrogram, proving that the varieties can be discriminated from each other based on the microsatellite allele sizes. Pedigree of Irsai Olivér and Mátrai muskotály has been confirmed by microsatellite allele size results, searching for the parents of Csabagyöngye and Királyleányka is in progress, since the molecular-marker based pedigree does not correspond with the putative origin of these cultivars. Our results excluded progeny-parent relationships in the
Csabagyöngye-Bronnerstraube-Muscat ottonel (Ottonel muskotály) and the Királyleányka-Kövérszőlő combinations. -
Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:250Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Examination for Susceptibility of CAE
33-36Views:95Caprine Arthritis Encephalitis (CAE) is a group of diseases in goats caused by a retrovirus, namely the Caprine Arthritis Encephalitis Virus. CAEV belongs to the lentivirus group within the retrovirus family. The CAEV is intimately associated with white blood cells, therfore any body secretions which contain white blood cells are a potential source of virus to other goats in the flock. Once goats are infected with CAEV, it remains infected for life and has to be eliminated.
Since not all goats infected with CAEV progress to disease, very important to test goats for infection using a serology test (ELISA, AGID, IDT). These serological tests demonstrate the presence of antibodies to CAEV in goat serum or detect the virus genome in the white blood cells. However, Swiss results (Dolf and Ruff, 1994) pointed out individual and species differencies in predisposition for this disease. This result was considered as a starting point to our examination. Microsatellite analysis was used in order to find whether there was any association between genotype and serological results, and to look for a marker associated with this disease. To date, altogether 135 goats have been examined. Unfortunately, a significant association between serological results and genotype was not found using the Chi2 test -
Phytopathological aspects of precision agriculture
135-139Views:86This paper illustrates the efforts based on the results obtained in the funding of precision agriculture, during more than two decades of cooperation between University of Debrecen and University of Oradea, within the framework of joint, EU co-financed projects, and put into practice on both sides of the border. Common plant-health databases, interactive Web pages, consultation activities, professional publications, professional training activities, laboratory infrastructure improvements, common research themes proves the progress made to date and create conditions for further development of joint research activities.