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Genetic discontinuity analysis of Collared Dove (Streptopelia decaocto)
5-11Views:188The Collared Dove conquered continent areas within a few decades. Causes and dispersion pattern of expansion has been investigated in several studies. However, the relationship between the geographic distribution and genetic structure of populations has not been researched. We used 152 individuals from 19 countries in this study. We analyze a 650 bp long mitochondrial COI sequences of each individuals. We were performed Spatial Autocorrelation Analysis, Principal Component Analysis and analysis of the genetic discontinuity in this study. Under 2500 km distance was a positive correlation between the genetic differentiation and different geographical areas. Hidden genetic barriers were found only Carpatian Basin. Could not be detected signs of genetic isolation in other regions. This will probably due to the unevenness of the sample collection, because these areas proportionally much fewer sequences were available. Therefore, is worth repeat this analysis after further sample collection, in the future.
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Water relations composition among Egyptian cotton genotypes under water deficit
5-15Views:207Background: water shortage is one of the major factor effects on growth characters and yield of most crops. Objective: this study was conducted to get to know the reactions of some Egyptian cotton genotypes to water deficit. Methods: The genetic materials used in this study included thirteen cotton genotypes belonging to Gossypium barbadense L., from the Cotton Research Institute (CRI), which was devoted to establishing the experimental materials for this investigation. Results: the ratio of GCA/SCA was less than unity for all studied indices, indicating predominance of non-additive gene action (dominance and epistasis), which is an important in exploitation of heterosis through hybrid breeding. Results: The data showed significant reduction in water relationship characters for all parental genotypes under stress conditions. The Egyptian variety Giza 68 gave high values for most water relationship characters. Data revealed that the greater the value of tolerance index is, the larger the yield reduction is under water deficit conditions and the higher the stress sensitivity is becoming. The parental genotypes Giza 96 showed the highest reduction in yield under water deficit conditions. At the same time, the cross combination Minufy x Australy showed higher values of yield reduction followed by the combinations Giza 67 x Australy. Of the male parents, the Russian genotype 10229 recorded the best GCA values for most water relationship characters. At the same time, the female parents, the old Egyptian genotype Giza 67 recorded the best values and exhibited good general combined for most water relationship characters. The cross combinations Giza 86 x Pima S6, Giza 77 x Pima S6, Giza 94 x Dandra and Giza 96 x Australy showed significant desirable SCA effect for most characters. Conclusion: relative water content %, osmotic pressure, chlorophyll and carotenoids content indicates better availability of water in the cell, which increases the photosynthetic rate. Also, the higher level of proline accumulation in the leaves which was recorded under deficit water suggests that the production of proline is probably a common response of plant under water deficit conditions.
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Some population genetics parameters of the present Hungarian Hucul Horse population
15-22Views:192We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.
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Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:173Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Introduction of DNA-based Methods to Agriculture Through Molecular Taxonomic Examination of Poa Species
139-142Views:117biological methods, one among them is AFLP that is well applicable for taxonomic research. Bluegrass species, that are important components of meadow associations, thus their thorough knowledge is necessary in maintaining biodiversity, were examined with bringing this method to perfection.
Taxonomic relationship of the members of Poa pratensis aggregation is a controversial issue. Present study aimes to identify the members of this group, with a developed AFLP method through molecular taxonomic examination of Poa species in meadows nearby Debrecen, revealing their genetical distances. Species of the aggregation show a great genetic variability, but their genetic proximity approves the use of the term aggregation. Results established wider geographical investigation of three species of the aggregation. The distinctness of the species based on their morphological features was confirmed according to their genetical basis as well. The method overall turned out to be appropriate for the taxonomic research of bluegrass species like Poa pratensis and its aggregation. -
Some strategic aspects of animal protein production
11-19Views:199The access to food shaped human societies and dietary models throughout the history of mankind. Animal protein consumption became a part of human culture. Data are presented showing the relationship of daily calorie and animal protein consumption as affected by capita GDP changes. Examples are presented how genetic improvement of animal and fodder plants influenced the resource efficiency and the overall environmental footprint per unit product. The two examples presented are: the dairy industry of the USA the 1944 and 2007 situation, and the Hungarian broiler chicken sector considering data relevant to 1930, 1960 and 2010. In both cases, dramatic improvements in resource efficiency could be demonstrated. The agricultural area required to animal feed production was reduced by more than 80% in both cases per unit product. Future possibilities are briefly discussed, referring to the still unutilized land reserves of the Globe, the new evolving technologies in progress inclusive the CASPR/Cas 9 genetic editing methods.
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The main influencing factors effecting the yield of maize
137-141Views:119Maize is one of Hungary’s major cereals. In the 1970s and 1980s, we were in the frontline regarding yields and genetic advancement. However, yield fluctuation in maize has increased to 50-60% from 10-20% since the 1980s, which was partly caused by the increase in weather extremes due to climate change and by agrotechnical shortcomings.
The experiments were carried out on typical meadow soil in four repetitions in the period of 2007-2008. In the sowing time experiment, sowing was performed on 10 April, 25 April, 15 May under a uniform fertilization of N120, P2O580 K2O 110 kg/ha. In the fertilization experiment, the yielding capacity of 10 hybrids with different genetic characteristics was studied in a control (non-fertilized) treatment and basic treatment of N40 P2O5 25, K2O 30 kg ha-1 active ingredient and a treatment with fivefold dosages of the basic treatment. In the plant density experiment, the relationship between plant density and yield was analysed at plant densities of 45, 60 and 75 thousand plants per ha. We found a tight correlation between sowing time and yield and grain moisture content at harvest. We found that grain moisture can be reduced by 5-10% by applying an earlier sowing time.
The agroecological optimum fertilizer dosage was N 40-120, P2O5 25-75, K2O 30-90 kg ha-1 active ingredient at a plant density of 60-90 thousand plants ha-1 depending on the hybrid and the year. -
The relationship between the nutrient supply and the yield of maize hybrids with different genetic traits on chernozem soil in variant years
27-31Views:216The experiments were set on lime-coated chernozem soil in 2013 and in 2014, in our study four hybrids were included with different FAO number. We studied the effect of NPK fertilization and row spacing on the yield. The fertilizer doses were based on a 25-year longterm experiment. Compared to control, the N40 +PK treatment has also achieved a significant yield increase, although some hybrids responsed with yield loss to the increasing fertilizer doses; this effect was observed especially in 2014. The majority of hybrids reached higher yields in both years using the 50 cm row spacing. The water release of hybrids was measured weekly during the maturation, at the same time points. The rainy September slowed ripening and the water release of the hybrids in 2013, so the grain wet content at harvest showed higher values. The moisture contents were increased for some hybrids, in spite of the positive and favorable dynamic of water loss.
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Unraveling changes in the duck microbiome and inflammatory processes due to allithiamine-enriched feed
163-171Views:130The gastrointestinal tract of poultry harbors a diverse and intricate microbiome that plays a crucial role in nutrient digestion and absorption, immune system development, and enhances resistance against pathogens. Maintaining a healthy state and proper production is fundamentally determined by the symbiosis between the host and microbes. Due to genetic and technological improvements, intensive growth rate can be associated with many pathological conditions, such as increased susceptibility to infections. Intestinal inflammation in poultry industries detrimentally affects productivity by hindering nutrient absorption and the efficient allocation of nutrients for growth. The host releases different biomarkers in response to inflammation. Hence, there is an utmost interest of reliable, precise, sensitive and robust biomarkers to evaluate both the gastrointestinal health status and inflammation in poultry. The aim of this study was to determine how the developed feed prototype (allithiamine) affects the community diversity in raised duck, and the relationship between gut microbiome composition and inflammatory factor as calprotectin, using targeted 16S rRNA gene amplicon sequencing and Chicken Calprotectin ELISA Kit.
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Agrobotanical Analysis of Bean (Phaseolus vulgaris L.) Equilibrium Populations Suitable for Regional Land Cultivation
148-152Views:74The local bean landraces maintained at small-scale farming level should have importance as complementary source for completion of plant genetic resources with potential role in local land cultivation. Also, they should be used as primary material in breeding activities targeted on improving the foodstuffs quality. In the research were used especially landraces collected in Nyírség, Körös-mellék and Tápióvidék region. Our main objective was to reveal the relationship between the adaptation ability of landraces and the distance from their original collecting place. For that reason the location of the experimental plots was choosen by taking into account that they must be checked in each region, on it’s original region and similarly in other two different regions. On the base of the recorded agro-morphological traits we can get answers on the shaping of adaptation ability, and in the same time by a general chemical characterisation we should know more about the variation of qualitative parameters with a potential economical role. In the designing of experiment a main importance had the conditions where the research is taking place. All activities related to the maintenance of experiment were done in frame of a low input agricultural system to emphasize as much as possible the natural endowments of regeneration places.