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SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
17-24Views:263The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers. -
Pre- and postharvest technologies to extend the shelf life of Prunus cerasus
85-89Views:210Fresh tart cherry consumption cannot be increased without the development of an appropriate technology for its elongated storage. This requires the development and optimization of the pre- and postharvest treatments. Currently, we have only limited knowledge about tart cherry (Prunus cerasus L.) postharvest technology, however, related studies on sweet cherry (Prunus avium L.) may be adopted. In this article, we have collected the most important research results in this topic.
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How does the S-locus determining self-incompatibility in stone fruits work in self-compatible peach?
93-100Views:88The majority of stone fruit species are self-incompatible, a feature that is determined by a specific recognition mechanism between the S-ribonuclease enzymes residing in the pistils and the F-box proteins expressed in the pollen tubes. Failure in the function of any component of this bipartite system resulted in self-compatibility (SC) in many cultivars of Prunus species. Peach (Prunus persica (L.) Batsch.) is the only species in the Prunoideae subfamily that is traditionally known to be self-compatible, but its molecular background is completely unknown. Isoelectric focusing and S-gene specific PCR revealed that SC is not due to functional inability of pistil ribonucleases. We hypothesize that SC may be a consequence of a kind of pollen-part mutation or the action of one or more currently unknown modifier gene(s). Only two S-alleles were identified in a set of peach genotypes of various origin and phenotypes in contrast to the 17–30 alleles described in self-incompatible fruit trees. Most important commercial cultivars carry the same S-allele and are in a homozygote state. This indicates the common origin of these cultivars and also the consequence of self-fertilization. According to the available information, this is the first report to elucidate the role of S-locus in the fertilization process of peach.
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The setting of fenological- stadium of plum (Prunus domestica) varieties in 2012
93-96Views:113We planted experimental trees, namely 6 plum varieties grafted on 6 plum rootstocks in the spring of 2010. Our aim was to observe differences in the fenological-stages of plum rootstock and variety combinations. ‘Cacanska lepotica’, ‘Jojo’,
‘Katinka’, ‘Topfive’, ‘Topper’, ‘Toptaste’ plum varieties were planted on ‘Mirobalan’ (Prunus ceresifera var. ceresifera cv. myrabolan); damson (Prunus institicia) – ‘St Julien A’, ‘St Julien GF655/2’; and ‘Wawit’; ‘Wangwnheim’; ‘Fereley’ rootstocks. We observed the bud burst, the flowering course: at the start of the flowering, during the main flowering, and at the end of flowerings and the ripening of the plum. Finally we observed the difference in leaves falling observed in the case of the different varieties and rootstock combinations.
The bursting of buds started with ‘Cacanska lepotica’, in March 16 and finished with ‘Jojo’ / ‘Mirobalan’ combination, in March 22. The starting of flowering course was in March 29 with ‘Topper’ / ‘St Julien GF655/2’ combinations and the end of flowering course finished with ‘Toptaste’ varieties, in April 3–4. The start of ripening of the plum was with ‘Katinka’ / ‘St Julien A’ combinations, in July 17. and the end of ripening of the plum finished with ‘Topper’ variety. The start of leaves falling began with ‘Cacanska lepotica’, in Sept 5–8 depending on irrigation, and ‘Katinka’, ‘Jojo’ varieties finished, in November 28–29.
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Determination methods of quality parameters of Prunus cerasus
107-111Views:138Sour cherry (Prunus cerasus) is a non-climacteric fruit. Storage optimization would enable the expansion of fresh consumption. The quality parameters are required for storage optimization. Those parameters are sugar content, acid composition, mineral content and alcohol content,
moreover it is also planned to determine antioxidants, vitamins and patulin mikotoxin under different conditions. In this paper, we demonstrate the measurement options of these parameters. -
Ideas on the European stone fruit yellows – as an entomologist can see them
30-34Views:172The European stone fruit yellows (ESFY) is an important endemic disease in Europe which causes in both, the Mediterranean countries and Central Europe serious damage. Its pathogen is the ‘Candidatus Phytoplasma prunorum’. The treatment and healing of the diseased trees and plantations with chemicals do not promise success. Thus, prevention may be the only solution. The transmission and spread of the pathogen happen by infected propagation material (grafting) or a vector (the psyllid, Cacopsylla pruni). Mechanism of the pathogen’s transmission and population dynamics of the vector have been extensively investigated in several European countries, which may allow by the control of C. pruni even to hold back the disease. Diseased stone fruit trees and wild Prunus spp. as main host species play an important role in maintaining and spreading the pathogen. C. pruni collects the pathogen by feeding on these plants and it carries persistently ‘Ca. P prunorum’. Researchers in Hungary have been characterized the disease only in terms of plant pathology, but neither the significance of the vector nor the role of wild Prunus spp. have been studied. This summary intends to give clues to these researches, that not only axe and saw should be the instruments of national control, but knowing the role and population dynamics of the vector the stone fruit production should be more successful.
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The bioactive compounds of sour cherry (Prunus cerasus) with special regards to antioxidant activity and antioxidant density
83-87Views:165Evidence suggests that a diet high in fruits and vegetables may reduce the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation.
In our work, we identified the total antioxidant capacity and ”antioxidant density” of sour cherry varieties named ”Újfehértói fürtös”, ”Debreceni bõtermõ”, ”Kántorjánosi” and ”Érdi bõtermõ” and those of the ”Bosnyák” sour cherry clones. ”Antioxidant density” is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, per 1 Calorie. -
Review of the biology of plant psyllid (Cacopsylla pruni, Scopoli 1763), and its role in the spreading of European stone fruit yellows, ESFY-phytoplasma with Hungarian data
25-33Views:295The European stone fruit yellows (ESFY) phytoplasma disease caused by pathogen ’Ca. Phytoplasma prunorum’ induces serious damages in cherry, sour cherry, peach, and apricot orchards mostly in Europe. Its known vector is the plum psyllid (Cacopsylla pruni). Many articles report on the biology (morphology, taxonomy, life cycle etc.) and the method of transmission of the pathogen by the vector, and the possibilities of their control. This paper reviews our knowledge about the vector, and summarises the results of an inland research carried out in a northeastern Hungarian apricot orchards. Our goal was to show some important data for the farmers or anyone who is interested in this disease and its vector. And give some known method that we can protect our orchards against them to prevent the appearance of the disease. As the psyllid that became infected with the pathogen can hold its infectionous capacity during their lifetime, it is very important to have enough knowledge about their lifecycle, that we can determine the right time and method to control them. We also have to know how to identify them; therefore, this paper lists several important data which can be helpful. The most important keys of identification are their wing color, which dark borwn in the apex and brown is in the remaining part of the forewing. The length of the antennae is also an important factor, since other genuse’s species have longer antennae than twice the width of the head. C. pruni has as long antennae as twice the width of the head. They return to Prunus species in early spring and we have to protect our orhards in this period against them. We have to use preparations with a knock down effect on them to prevent the inoculation of the pathogen into the trees in our orchards.
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Correlation of pruning time and fruiting part differentiation of sweet cherry cultivars
131-134Views:65There are several extended studies in sweet cherry production in Hungary and all over the world i.e. for creation and maintainence of smaller tree crown and high density orchards. The use of suitable dwarf rootstocks for this fruit species are very limited. On one hand, most of the draf rootstocks do not cause enough growth reduction and on the other hand these rootstocks are get old very quikcly and their fruits become small, and therefore, they not serve the requirements for intensification. In summary, there is a need for those rootstock which are vital, regeneration enhanching and delay ageing. Due to ensuring above features, Prunus mahaleb is still an obvious solution for intesive production. Increasing intensification can be obtained by use of modernisation of technological elements and suitable cultivar choice.
According to this increasement of intensity through application of novel technological elements (timing, manner and severity of pruning) and selection of the proper cultivar is implement able. Important differences are experienced between sweet cherry cultivars in their growth attributes, light demand and dynamics of fall back in regenerative potencial of different aged wood parts. From this point knowledge of the abow detailed is very important in order to maintain rentability of already established plantations. Our work shows the formation of production part in
9-year old plantation with spike spindle and free spindle crown forms depending on pruning timing (winter, summer) and determining of their various effects. -
A forgotten sour cherry pest, the stone fruit weevil (Anthonomus /Furcipes/ rectirostis L.) appeared again
104-106Views:68The stone fruit weewil (Anthonomus rectirostris L.) has been known as the kernel pest of the wild cherry in Hungary. There have been no data about its harm on sour cherry (Prunus cerasus L.) in our country, yet. 5-10% of stone infection has been observed on some sour cherry trees (cultivars: Debreceni bőtermő, Újfehértói fürtös) in the eastern side of an orchard at Debrecen-Józsa adjacent to a wood in early July 2011. The damage can be in connection with the fact that the yield has not been harvested for years.
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The olive (Olea europaea) and the almond (Prunus amygdalus) related phytonutrients, and the associated health-promoting biological effects, a review
11-24Views:208With the increasing attention to the health promoting activities of the bioactive compounds from some plants, many researchers are focusing on the biological potential and mechanisms of certain cultivated plant species. In this review, we survey the olive and almond based extracts specific phytoconstituents and their associated health promoting effects that have been evaluated in experimental and clinical studies.
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Effect of Storage on Fruitquality of Apricot (Prunus Armeniaca)
164-169Views:96The quality of a product is determined by numerous characteristics. As quality characteristics are polygenic, they cannot be improved easily. Moreover, there often is a negative correlation between the different parameters in the case of apricot. The firmness of fruits decreases with increasing size. The year effect also has a great influence on the expression of the potential quality of the given cultivar.
Our examinations were carried out in 2004 at Boldogkőváralja, in the cold storage plant of the Northcot Ltd. We monitored the development of fruits from fructification until harvest, and also the quality changes during storage. 100 fruits were observed from each cultivar directly after harvest. We measured height, width, weight, firmness, dry matter content and seed weight. From each cultivar, 100 fruits were stored, of which 25 fruits were observed each week. The value and the dynamics of the changes in the parameters compared to the values at harvest varied greatly for the different cultivars. Strong correlations between the parameters have not been found within one cultivar either. The heavier fruits did not lose more weight, either in absolute value or relatively, than the lighter fruits. The increase in the firmness of fruits was not accompanied with a desirable decrease in the dry matter content. -
The setting of fenological-stadium of plum (Prunus domestica) rootstock-variety combinations in 2011–2013
25-29Views:102We planted experimental trees, namely 6 plum varieties and 6 plum rootstocks in the spring of 2010, which of aim were the phenological -phases of variety-rootstock combinations analyses.
We observed 18 plum varieties – rootstocks combination in Kecskemét, in Garden of Kecskemét Collage. We set the examination with 2 kind irrigations. A plum combination appear in the examination in 6 repetition depend on irrigation. The vegetative period started bursting of buds in 2011 March 15–18.; 2012 March 16–19. and 2013 March 12.–April 2. This was followed green buds stadium shorter or longer periods, than appeared white buds stadium and in the following day we could see started of flowering. The main flowering kept 7–10 days, except in 2012, when arriving frozen by April 8. morning stopped the main flowering. Following flowering we observed fruit falling 3 occasion, these weren’t noticed calendar date. These were in order: fruit falling after set, fruit falling in June, fruits falling before harvest. In 2013 these fruit falling there weren’t considerable. The ripening was characteristically varieties. The most of earlier ripening was ‘Katinka’/‘St Julien A‘, from which we didn’t harvest in 2011, but in 2012 July 17, and in 2013 July 9. pick up some plum fruits. The ‘Cacanska lepotica’ ripped in July 21–30, this was followed in ripening time the ‘Topfive’ in July 19.–August 6., The ‘Toptaste’ in August 5–23., The ‘Jojo’ in August 2–26., and the ‘Topper and ‘Katinka’/‘Mirobalan ’ stopped ripening in August 22.–September begin. The colouring leaves and the falling leaves started continue after harvesting, but we experienced the end of the falling leaves in the first bigger frozen time, October 24.–November 26.
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The effect Benefit PZ biostimulant on the fruit size and yield production of apricot varieties (Prunus Armeniaca)
16-18Views:87Fruit size is determined by the genetic makings of varieties the pomology and enviromental factors. The climate and the previous year’s harvested yield have a great effect on yield volume. It is not easy to harmonize the qualitative and quantitave parameters of the fruit. High yield usually causes smaller fruits.
The aim of our trials was to evalaute the effect of Benefit PZ biostimulant on fruit size. We examined two apricot varieties (Bergeron, Magyarkajszi C235) for the efficacy of the product. The three applications were made with 400 l/ha water with 2500 ppm. The optimal interval of application with Benefit PZ is during in the early phases of development. The size of the final fruits depends on the number of cells making up the fruits. The later application could not increase fruit size growth, there are no significant differences between the Benefit PZ sprayed and the control plots. The totally harvested fruit weight was 13.5% more on the trees of cv. Bergeron, it was caused by the higher fruit set. On the sprayed trees were 12.2% more fruit. Therefore were no differences between the average fruit weight. Fruit weights of the treated trees were 44.37 g on cv. Bergeron, 44.15 g on cv. Magyarkajszi C235 and on the no treated trees were 43.83 g as well as 45.33 g.