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Estimation of direct and maternal genetic parameters for weaning weight in Hungarian Simmental cattle
17-22Views:175The aim of the current research was to estimate variance components and genetic parameters of weaning weight in Hungarian Simmental cattle. Weaning weight records were obtained from the Association of Hungarian Simmental Breeders. The dataset comprised of 44,278 animals born from 1975 to 2020. The data was analyzed using the restricted maximum likelihood methodology of the Wombat software. We fitted a total of six models to the weaning weight data of Hungarian Simmental cattle. Models ranged from a simple model with animals as the only random effect to a model that had maternal environmental effects as additional random effects as well as direct maternal genetic covariance. Fixed effects in the model comprised of herd, birth year, calving order and sex. Likelihood ratio test was used to determine the best fit model for the data. Results indicated that allowing for direct-maternal genetic covariance increases the direct and maternal effect dramatically. The best fit model had direct and maternal genetic effects as the only random effect with non-zero direct-maternal genetic correlation. Direct heritability, maternal heritability and direct maternal correlation of the best fit model was 0.57, 0.16 and -0.78 respectively. The result indicates that problem of (co-)sampling variation occurs when attempting to partition additive genetic variance into direct and maternal components.
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New Integrated Breeding Evaluation Method Used for German Warm-blooded Horses
3-6Views:116In Germany, the new integrated breeding value estimation is based on a multiple-trait animal model, considering simultaneously information from performance test of stallion on station, performance test of mares as well as competition results of sport horses. The objective of this study was to estimated the genetic parameters of the traits used in the integrated breeding evaluation including all German warmblood breeds. The analysed data consisted of 4527, 40670 and six million records for performance tests of stallions, mares and for competition results, respectively. Genetic parameters were estimated with a multivariate BLUP animal model. Heritabilities for traits obtained from stallions tested on station ranged from 0.33 (jumping under rider) to 0.51 (trot) and for the traits from mare performance test from 0.27 (walk) to 0.38 (trot). Genetic correlations between corresponding traits recorded on performance test of stallions and mares ranged from 0.87 (canter) to 0.98 (free jumping). Heritabilities of competition traits from horses (build up for sport) were estimated as 0.12 and 0.11 for dressage and jumping, respectively. The use of all traits for estimation of genetic values of horses is expected to reduce pre-selection and optimal combines all information resources based on the estimated genetic parameters.
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Estimation of genetic parameters and genotype effects for crossing constructions of Hungarian Pigs
57-60Views:156Authors estimated the genetic parameters and genotype effects of average daily gain (ADG), age (AGE) and lean meat percentage (LMP) using the field test data of Pietrain (Pi), Duroc (Du), Hampshire (Ha) pigs and their crosses. Data was collected by the Agricultural Agency of Administration between 1998 and 2010 originating from 68 herds. Datasets of the different crossing combinations (Pi, Du, Pi × Du; Pi, Ha Pi × Ha) were evaluated separately using bivariate animal models. The estimated heritabilities were moderately low: 0.24–0.29, 0.22–0.26 and 0.18–0.19 for average daily gain (ADG), age (AGE) and lean meat percentage (LMP), respectively. The estimated genetic correlation coefficients were negligible: -0.07–0.01 (ADG-LMP), -0.01–0.04 (AGE-LMP). The Pi × Ha and Pi × Du crosses showed 6.76% and 4.96%; 6.74% and 4,17% and 0.08% and 0.44% heterosis for ADG, AGE and LMP, respectively. Among the environmental factors the herd effects were substantial: 41.17%, 53.67% and 14.16% for AGD, AGE and LMP, respectively. The smallest environmental influences were found for LMP.
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Random regression models for genetic evaluation of performance of the Hungarian show-jumping horse population
87-91Views:148The aim of the study was to estimate genetic parameters for show-jumping competition performance using random regression model. Show-jumping competition results collected between 1996 and 2009 were analyzed. The database contained 272 951 starts of 8020 horses. Identity number and gender of the horse, rider, competition date, the level of the competition and placing were recorded in the database. Competition levels were categorized into five groups. Weighted – competition level used – square root transformed placing was used to measure performance of horses. The random regression model included fixed effects for gender, year and place of competition, and random effects for rider, animal and permanent environment.
Later performance of show-jumping horses measured with weighted square root ranks is less influenced by rider and permanent environmental effects than performance at the beginning of a horse’s sporting career. Heritability increased continuously from 6.3 years of age (2296 age in days), values were in the range of 0.07 and 0.37. Higher heritability was found in later ages. Weak genetic and phenotypic correlation was found between the early 4–5–6 years of age and older (7, 8, 8+) age classes. From 8.5 years of age (3132 days old) there were strong genetic and phenotypic correlations between neighboring age groups. For the same age classes moderate and strong genetic and phenotypic correlation was found. Genetic correlation between 13.5 years of age and older horses was very strong.
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Estimating Genetic Parameters using a Random Regression Model
53-55Views:135One of the most important part of the genetic evaluation using a random regression model is the estimation of variance components. This is the topic of many papers because the large computational costs. We can use restricted maximum likelihood (REML), Gibbs sampling and ℜ method for the estimation of genetic parameters. The variance components are necessary to calculate the heritabilities and repeatabilities.
The aim of our paper is to estimate the variance components using a random regression repeatability model from test day data set of Hungarian Holstein-Friesian dairy cows and to analyse the change of additive genetic and permanent environmental variance, heritability and repeatability over lactation. -
Genetic progress in winter wheat quality and quantity parameters
71-75Views:173Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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Population genetic results of Hungarian Botrytis cinerea isolates establishing new technologies with decreased chemical usage against grey mould
259-261Views:127Botrytis cinerea causes gray mold on a high number of crop plants. Information about the populations of plant pathogen fungi may help to develop new strategies for the effective and economic crop protection with reduced fungicide usage. Hungarian B. cinerea populations were characterized with using different molecular genetic parameters. B. cinerea group I strains, characterized with high rate of fenhexamid resistance, could be detected only in restricted number. The Hungarian B. cinerea populations were characterized with high genetic diversity, and the regular occurrence of sexual reproduction. These results highlight the importance of rotating different type of fungicide in the plant protection technology against grey mould.
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The effect of immigration on some population genetic parameters of the Hungarian Hucul population
91-96Views:151Population genetic indicators of the Hungarian Hucul population were calculated taking also into account the effect of imported horses.The birth year of the examined 3002 individuals ranged between 1871–2015. The last year involved in the examination was 2015, when the research was conducted on the stock under stud book control. The calculations on the population were made using the statistic indicators in the Endog programme, with and without the imported Polish horses. Figures were depicted using R software. Number of offspring of the Polish individuals was presented in tables; the distribution of the offspring per stud and per mare family, as well as the Nei’s genetic distance,were presented graphically.
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Some population genetics parameters of the present Hungarian Hucul Horse population
15-22Views:183We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.
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The significance of biological bases in maize production
61-65Views:179The comparative trial has been set up in the Demonstration Garden of the Institute of Crop Sciences of the University of Debrecen, Centre for Agricultural and Applied Economic Studies, Faculty of Agricultural and Food Sciences and Environmental Management in 2012, with 24 hybrids with different genetic characteristics and growing periods. The soil of the trial is lime-coated chernozem, with a humus layer of 50–70 cm.
The weather of the trial year was quite droughty; the monthly average temperature was 3–4 oC higher than the average of 30 years. High temperature, together with lack of precipitation occurred during the most sensitive phenophases of maize (flowering; fecundation, grain saturation).
The following characteristics have been observed: starting vigour, date of male and female flowering, plant and cob height, dry-down dynamics during maturation and the change of yield composing elements has also been quantified. The yield was recalculated to 14% moisture content grain yield after harvesting.
The beginning of the growing period was advantageous, therefore the analysed hybrids could grow a high (above 300 cm) and strong stem. The yield of the hybrids changed between 10.33 and 11.87 t ha-1, but as a result of the unfavourable climatic extremes, their genetic yield potential prevailed only at a rate of 30–40%. However, moisture content by the time of harvesting was good despite its early date (12th September); it remained under below 14% in most cases. Dry-down was measured on a weekly basis between 14th August and 5th September.
The analysis of the qualitative parameters of the maize hybrids (protein %, oil % and starch %) resulted in significant differences. The most significant difference has been observed in the case of protein content (LSD5%=2.01). Oil content was the most advantageous in the case of hybrids belonging to the mid-late growing group (FAO 400). The X9N655 and 36V74 hybrids had the highest oil content (around 4%), while hybrids P9915 and 37F73 had significantly lower oil content. Starch content was above 70% in the case of every hybrid.
Hybrid selection is highly important in terms of yield and yield security of maize, as well as the application of modern biological fundamentals and hybrid specific technology for the improvement of the level of cultivation technology.
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Application of GIS, precision agriculture and unplugging cultivation in plant breeding of Karcag
49-56Views:187In the last two decades, the prevailing ecological conditions and climate change have caused negative effects. Therefore, a paradigm shift is needed in the field growing of plants. The latest inventions, digital technologies, precision cultivation are not enough, the mentality of the farmers is more important. For this reason, not only big financial sacrifices, but adequate receptivity are needed on behalf of farmers. Adequate skills and continuous self-education are necessary. The yield of plant growing farms is determined by ecological conditions to a 40% extent, genetic background of the seed has a 30% share and the applied agricultural technology has a proportion of 30%. In different agroecological conditions, bred varieties of plants have bigger tolerance to unfavorable factors of the regions and significant yield stability. Farmers, who buy and sow seeds, can only contribute to the genetic potential of the seeds with cultivation technology. Plant breeding provides stable genetic background and good quality seeds. Breeding activity – choosing variety proposants, breeding them, selection work, classical breeding process for 8-10 years – must create new landraces, which can produce balance, high yield and have good quality parameters in extreme ecological conditions, yearly excursion and have higher tolerance to unfavorable factors of the region giving significant production stability for farmers. In Karcag GIS technology, precision cultivation elements and soil-friendly agrotechnical methods have been introduced which largely support the aims of breeding and can also provide optimal cultivation conditions in extreme years. Because of the specificity of breeding the main aim is not only to increase yield but to provide harmonic growing for bred materials, to decrease the number and the cost of cultivation and to be punctual. In this study, applied new methods and technologies are introduced.
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Study the correlation of morphological and production traits of honey bee (Apis mellifera L.) (Review
61-65Views:224There are several morphological differences among the honey bee subspecies, which proofs that they adapted different way to the environmental factors of the given area. Morphological observations are not the only way to separate subspecies, it is possible on their genetic speciality as well. The authors’s aim in this present study was to examine morphological characteristics of subspecies, furthermore looking for correlation between morphological parameters and honey yield. According to their results, among the most important parameters considered by Hungarian breeders, in the case of the native Apismelliferacarnica subspecies, the proboscis length could be the indirect tool for honey-productivity focused selection.
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Effect of divided nitrogen and sulfur fertilization on the quality of winter wheat
27-31Views:190The ecological characteristics and agro-ecological conditions in Hungary provide opportunities for quality wheat production. For the successful wheat production besides the favorable conditions; the proper use of expertise and appropriate cultivation techniques are not negligible. Successful cultivation affected by many factors. To some extent we can affect, influence and convert the abiotic factors.
Today, a particularly topical issue is the question of nutrition and that the species’ genetic code can be validated using the appropriate quantity and quality fertilizer. Beyond determining the fertilizer requirements of the winter wheat it is important to align the nutrient to the plant’s nutrient uptake dynamics and to ensure its shared dispensing. In any case, it is important to note the use of autumnal base-fertilizer as complex fertilizer. Hereafter sharing the fertilizer during the growing season with the recommended adequate nitrogen dose.The first top dressing of winter wheat in early spring (the time of tillering) can be made, the second top dressing at the time of stem elongation, and the third top dressing at the end of the blooming can be justified. Determining the rate of fertilizer application depends on the habitat conditions and the specific nutrient needs of plants. In autumn the 1/3 of the planned amount of basic fertilizer should be dispensed (in case of N). During setting our experiment we used 3 doses (0 kg ha-1 N-1 active ingredient; 90 kg ha-1 N-1 active ingredients and 150 kg ha-1 N-1 active ingredient). Application dates beyond the autumn basic fertilization are the following: in one pass in early spring, divided in early spring and the time of run up, early spring and late flowering. In addition to nitrogen the replacement of sulfur gets a prominent role as a result of decreased atmospheric inputs. The proper sulfur supply mainly affects the quality parameters. It influences positively the wheat flour’s measure of value characteristics (gluten properties, volume of bread, dough rheology.
In terms of nitrogen doses; the larger amounts (150 kg ha-1 N-1 drug), is the proposed distributed application, while in the case of lower nitrogen (90 kg ha-1 N-1 drug) in a single pass in the early spring can achieve better results. After using sulfur the quality values among the nutritional parameters that can be associated with gluten properties took up higher values than the samples not treated with sulfur.
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Simulation Experiments for Observation of Different Genetic Parameters
46-49Views:98The change of genetics variance in various simulated population were examined trough 25 generations. The population size were 1000, 2000, 4000, 8000 and 16000. The number of genes were1, 2, 3, 4 and 5. Two allels per gene were applied. It was assumed that base population was randomly mated. Also it was assumed that a pair of allel genes was independent, there was no epistasis, linkage between alleles of different loci, dominance and overlapping generations. In the selection was kept the 93% of females and 0.186% of males. The size of populations was close to constant through generations.
The size of populations and the number of loci were effects that how many generation is necessary to get genetical maximum. The change of the genetical mean of the population and the genetical variance were well subscribed cube and logistical curve. We can use to estimate these genetical parameters with the previous functons. -
Effects of Site on Winter Wheat Quality 2002/2003
100-107Views:140The demand of modern societies for high food quality is evident. Thus, it is important for agriculture to produce row materials that are valuable for nutrition and have favourable characteristics for food processing. For this we need a knowledge about the factors which determine the quality of products. One of the main features of plant production is the “immobility”. This way the characteristics of the field influence the quality of the product, like example winter wheat, which is the main cereal in Hungary and Europe.
The Concordia Co. has charged the Central Laboratory of Debrecen University, Agricultural Centre with laboratory testing of the 2002/2003 winter wheat crop. The samples consist of thirteen winter wheat varieties from six different sites under the same cultivating conditions. Therefore, the important wheat quality factors were analysed solely against site conditions with the use of Győri’s “Z” index, which contains these parameters.
Soils were tested first. In this experiment excepting the negligible differences between the sites, there were no linear relations found between quality factors, productivity and soil features. The case is the same with the relation between precipitation, temperature and quality parameters. However, it must be noted that additional soil analyses are required to interpret the extreme results obtained from Karcag.
The calculated Győri’s Z-index shows relative stability concerning certain varieties, although considerable deviation can be found in varieties related to the sites. According to these results, it can bestated that winter wheat quality was not linearly influenced by soil and weather in the 2002/2003 vegetation period. As the same cultivation technology was used in the experiment, the index was determined by genetic features. It must be noted that these findings are relevant only to this experiment. -
Evaluation of blue and red corn landraces
239-243Views:135Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
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Agrobotanical Analysis of Bean (Phaseolus vulgaris L.) Equilibrium Populations Suitable for Regional Land Cultivation
148-152Views:72The local bean landraces maintained at small-scale farming level should have importance as complementary source for completion of plant genetic resources with potential role in local land cultivation. Also, they should be used as primary material in breeding activities targeted on improving the foodstuffs quality. In the research were used especially landraces collected in Nyírség, Körös-mellék and Tápióvidék region. Our main objective was to reveal the relationship between the adaptation ability of landraces and the distance from their original collecting place. For that reason the location of the experimental plots was choosen by taking into account that they must be checked in each region, on it’s original region and similarly in other two different regions. On the base of the recorded agro-morphological traits we can get answers on the shaping of adaptation ability, and in the same time by a general chemical characterisation we should know more about the variation of qualitative parameters with a potential economical role. In the designing of experiment a main importance had the conditions where the research is taking place. All activities related to the maintenance of experiment were done in frame of a low input agricultural system to emphasize as much as possible the natural endowments of regeneration places.
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Morpometric study of Hungarian honey bee (Apis mellifera L.) colonies
59-63Views:308The honey bees are essential for the pollination of agricultural plants. The Pannonian honey bee, Apis mellifera pannonica, is native to Hungary, only these subspecies are being bread in our country. The parameters have been separated the pannon and italian honey bee subspecies, the colour of tergit, the cubital index and proboscis length. The morphometric analisys is of special importance because this, on the one hand, shows correlation with honey bee production and on the other hand, the pure morphometric charactersitics is the basis of any potential honey bee export. Mitochondrial DNA and microsatellites are the common methods to define genetic diversity and the separation of subspecies.
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Fruit quality of sweet cherry cultivars in superintensive orchards
75-81Views:260The fruit quality of 15 sweet cherry cultivars (’Canada Giant’, ’Celeste’, ’Chelan’, ’Ferrovia’, ’Germersdorfi Rigle’, ’Katalin’, ’Karina’, ’Kordia’, ’Linda’, ’Regina’, ’Sam’, ’Sandra Rose’, ’Sunburst’, ’Sylvia’ and ’Techlovan’) was studied under super-intensive growing conditions at Nagykutas. We measured the fruit diameter, fruit width, fruit height, stem length and stem weight, fruit and pit weight and the total dry matter content. There were large differences among the cultivars. These differences are due to the genetic characteristics of fruits because all other conditions were the same. For 11 cultivars, we collected fruit samples several times /2-4/. We examined on this cultivars all the above listed fruit quality parameters. When examining these samples, we have gained information how earlier or later than optimal harvest time influences fruit quality.
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Sweet cherry fruit quality by half-intensive orchard management
76-79Views:108We made our study on fruit quality in the summer of 2005 on 6 cultivars („Bigarreau Burlat”, „Germersdorfi 3”, „Germersdorfi 45”, „Katalin”, „Linda” and „Van”) in a half-intensive sweet cherry orchard in Siófok. We measured fruit diameter, fruit width, fruit height, stem length and stem weight, fruit and pit weight and total soluble solids content. There were large differences among cultivars in the datas. These differences are due to genetic fruit-characteristics, because all other conditions were the same. We harvested fruits by 3 cultivars two times to check their characteristics on two ripening phases. We examined on these cultivars all the above listed fruit quality parameters. With these data we can be informed about the role of later than optimal harvesting time and how big an effect it has on fruit quality.
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The effect Benefit PZ biostimulant on the fruit size and yield production of apricot varieties (Prunus Armeniaca)
16-18Views:141Fruit size is determined by the genetic makings of varieties the pomology and enviromental factors. The climate and the previous year’s harvested yield have a great effect on yield volume. It is not easy to harmonize the qualitative and quantitave parameters of the fruit. High yield usually causes smaller fruits.
The aim of our trials was to evalaute the effect of Benefit PZ biostimulant on fruit size. We examined two apricot varieties (Bergeron, Magyarkajszi C235) for the efficacy of the product. The three applications were made with 400 l/ha water with 2500 ppm. The optimal interval of application with Benefit PZ is during in the early phases of development. The size of the final fruits depends on the number of cells making up the fruits. The later application could not increase fruit size growth, there are no significant differences between the Benefit PZ sprayed and the control plots. The totally harvested fruit weight was 13.5% more on the trees of cv. Bergeron, it was caused by the higher fruit set. On the sprayed trees were 12.2% more fruit. Therefore were no differences between the average fruit weight. Fruit weights of the treated trees were 44.37 g on cv. Bergeron, 44.15 g on cv. Magyarkajszi C235 and on the no treated trees were 43.83 g as well as 45.33 g. -
Andrological examination of Hungarian Large White and Landrace boars
5-10Views:237The Hungarian Large White and Hungarian Landrace pig breeds have outstanding lifetime performance, production parameters and crossbreeding ability. Nevertheless, during the recent decades, these breeds could not compete with the West European hybrid pigs concerning on production results. In this study, we made a complex andrological evaluation of boars in different Hungarian nucleus breeding stocks. After taking blood and semen samples, performing gonadotropin-releasing hormone (GnRH) challenge, ultrasonic and thermographic examinations were done. Laboratory tests were accomplished in reproduction labs of NARIC ABNMS. Our goal was to determine the reproductive performances of boars and collect samples for future genetic examinations, respectively.
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Examination of the Effect of Cropyear on the Yield Potential and Yield Stability of Winter Wheat Varieties
62-67Views:150Variety selection is one of the most important, determinative elements of sustainable winter wheat production. Yield potential, and yield stability are the most important elements in the variety selection of winter wheat, but baking quality parameters play an important role, too.
Several winter wheat varieties were tested for yield and yield stability on chernozem soil in the Hajdúság (in the eastern part of Hungary), in the 2001-2002-2003-2004 cropyears. The management factors were the same for all cropyears. 15 varieties in early the maturity group, 14 varieties in the middle maturity group and 4 varieties in the late maturity group were tested in the above mentioned cropyears. The climatic conditions were average in 2001, dry in 2002, extremely dry in 2003, and very favourable in 2004.
We obtained 5298-6183 kgha-1 yield from early maturity varieties, 5683-6495 kgha-1 from middle, 5694-6031 kgha-1 from late ones in the average of four years. The cropyears had strong influence on the yields, even on chernozem soil, and were characterized by excellent water – and nutrient – husbandry. Averaging of cropyears and genotypes, we obtained 6984 kgha-1 in 2001 (average cropyear), 5452 kgha-1 in 2002 (dry cropyear), 3120 kgha-1 in 2003 (extremely dry cropyear) and 8400 kgha-1 in 2004 (optimum cropyear), respectively. The yield differences between the minimum and maximum yields were 885 kgha-1 in early varieties, 812 kgha-1 in middle and 337 kgha-1 in late maturity varieties, respectively. The varieties characterized by high yield potential and the varieties characterized by good yield stability were different, so in variety selection we have to take both genetic traits into consideration. There were positive, significant correlations among the yields of winter wheat varieties (early, middle, late), the temperature of spring months. (March-April), and the rainfall of spring months (March-April) (R2=0,703**-0,768** and R2=0,681**-0,749**, respectively). We found a high negative correlation between the temperature of early summer months (May-June) and the yields of wheat varieties (R2= -0,856**- -0,918**).
According to the results of our experiment, it is very important to harmonize yield potential and yield stability in the variety selection of winter wheat. -
Examination of the usability of the MALDI-TOF method in sunflower genetic identity analyses
33-38Views:187Economically, one of our most important crop is the sunflower. Regarding its production it is essential to use top quality seeds. As the conditions of seed certification and the certification parameters are regulated by laws and ordinances, it is highly important to farmers and seed producers to detect seeds of low quality or dubious origins.
Nowadays, the examination of the cultivar homogenity of sunflower is based on a reference method of the International Seed Testing Association Rules International (ISTA), Chapter 8. This standard reference method uses ultrathin poliakrilamid isoelectric focusing gel electrophoresis (IEF-UTL, or simply IEF). With this work we have set out to compare the results of MALDI-TOF to the test results – used as reference results – achieved by this reference method. The aim is to develop a new, quick, cheap and reliable method. In this article I summarized the results of some of the experiments that will provide basis for my further work.
During our previous experiments we have concluded that out of four extracting agents we can get the most protein markers using NaCl acid buffer, 1 propanol buffer.