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Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:468The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally. -
N-fertilization using „Biofert” in Sustainable Maize Production
30-33Views:611In synthetic fermentation of lysine (amino-acid) a by-product (Biofert) originates which can be characterized by 6% N-content and other ingredients (vitamins, enzymes, micro-elements etc). In small and large plot experiments Biofert was studied in different agroecological (cropyear, soil), biological (genotypes) and agrotechnical (non-irrigated and irrigated; N-splitting etc) conditions in order to obtain information about agronomic efficiency and environmental effects of its applications.
Our results proved that Biofert has the same agronomic efficiency as traditional N-fertilizers (applied in equal doses and splitting), but Biofert has economic and environmental advantages (less N-leaching in soils) for maize production. We found a special interaction between N-supply and irrigation. In maize production (irrigation) with the optimum application of nutrient- (N-fertilization, Biofert) and water- supply we could stabilize maize yields at a high level (11.0-14.0 t/ha) fairly independently of agroecological factors. When applying Biofert in autumn, NO3-N leaching was less in 100-200 cm chernozem soil-layers than for applications of traditional N-fertilizer. There were no differences between different maize genotypes concerning the agronomic efficiency of Biofert. In maize production 120-190 kg/ha N (chernozem soil) and 165 kg/ha N (meadow soil) doses of Biofert were the optimum doses in splitting applications (autumn + spring). -
The Usage of New Multispectral Satellites in Agro-Environmental Protection
282-289Views:581GIS, and especially remote sensing, offers great help in performing agro-environmental protection tasks. It can process a large amount of data to an arranged set of databases, and is also an excellent source of information. Moreover its keeps the geographical coordinates of all data during processing. The usage of remotely-sensed data is one of the most up-to-date and effective ways to observe, analyze and understand the complex phenomena taking place in all the spheres of agricultural production (soil, water, air), and also to track and monitor the changes of different environmental parameters, as they constantly change in time and space. Several indices will be described, which can be derived from remote sensed data. Next, a relatively new hyperspectral satellite sensor (MODIS) will be introduced, as it can be a input data source in research performed in agri-environmental protection. Last, a new meteorological satellite (MSG-1) will be introduced, as its data are available for public usage, and it could be an important data source.
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Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
81-85Views:643In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking water bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium. -
Stevia (Stevia rebaudiana B.) yield in the case of different production technologies
71-77Views:455Stevia rebaudiana B. offers a natural alternative of sweetening, potential health promotion plant, and our country shows increased interest about cultivation in Hungary in addition to the neighboring European countries. The agricultural production system installation necessary
understanding of the needs of the plant, as well as exploring the agronomic potential. Field experiments were conducted in 2015 with the aim to highlight some of the technological production parameters and correlations between them. We have reviewed the most important agronomic factors, the spacing (50×50 cm and 33×33 cm), ground covering (agro-cloth covered and uncovered/ control), their vegetative growth (first-,second-order branch), herb yield and quality in effect.
Based on empirical evidence, that the Hungarian climates also have the opportunity of 3 cuttings during a growing season, besides of early planting, optimal climatic conditions (in October didn’t reduce the daily minimum temperature below 0 °C, after cuttings the maximum interval values remain below 40 °C), and adequate water supply and crop protection facility. The 33×33 cm spacing evaporates more than 50×50 cm spacing, because of the dense population, the continuous canopy less able to breathe, so there are serious chances to the pathogen colonization.
Based on the results of our research to the wider 50×50 cm spacing favorable appreciate the stevia optimal progress in terms of qualitative and quantitative parameters of the yield. The stevia yields produced in the Northern Great Plains field cultivation can produce similar results as stevia crop yields in warmer climates, where the primary crops. Our research experience suggest that there is a viable domestic stevia cultivation, developing the necessary technology is still growing further investigation justifies. -
Effect of plant growth promoting Rhizobacteria (PGPRS) on yield and quality of processing tomato under water deficiency
19-22Views:545Chlorophyll fluorescence was measured of H1015 tomato hybrid with different bacterial treatments (B0–B1–B2–B3) and three irrigation treatments: regular irrigated (RI), deficit irrigated (DI) and non-irrigated conditions (I0). The aim of the experiments was to show the effects of plant growth promoting rhizobacteria on the yield, dry matter and vitamin C content of processing tomato during different irrigation treatments, and measuring the chlorophyll fluorescence during the ripening and development stages. According to the results, none of the bacterial treatments had a statistical effect on the quantity and quality of the tomato and on the chlorophyll fluorescence, only the irrigation. Further studies are needed.
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The Effect of Atmosperical Aridity on the Changes of Quantitative Parameters of Horticultural and Arable Crops
40-45Views:397The occurence of atmospherical drought causes serious water-supply problems in the most cases of our domestic agricultural plant species. This paper was studied, how can we quantificate the atmospherical drought, with the help of a low input (relative humidityof the air, temperature) index. If this index (LSZI) characterized the atmospherical drought well, it will suitable to estimate the yield amount of agricultural plants.
The index elaborated by the authors was tested on county average crop yield of 14 agricultural plant species. Moreover we compared the atmospherical drought index (LSZI) to other aridity parameters, how suitable for estimate the yield amount.
Result of experiments show that, the atmospherical drought index (LSZI) can be used well by several agricultural plant species in especially coern and sugar-beet to estimate yield amount. Excellent results were found by comparison to other aridity indexes, this means it is worth using in the aridity researches in the future. -
Effect of crop residues on soil aggregate stability
23-32Views:219Soil structure may be improved by adding readily decomposable organic matter. The extent of amelioration depends on the chemical build-up and decomposability of the crop residues. Three different kinds of organic matters were investigated: (1) maize stem, (2) wheat straw, and (3) maize stem
& wheat straw. Comparing the aggregate stabilizing effects of the differently decomposable organic matters to each other, the expected maize stem & wheat straw (mw) > maize stem (m) > wheat straw (w) order was proved. -
The effect of cobalt-chloride on the production parameters and homogenity of barramundi (Lates calcarifer) larvae
21-25Views:505Barramundi (Lates calcarifer) is a predatory fish species native in Southeast Asia and Australia. Based on the geothermal potentiality of Hungary the high market potential warm-water fish barramundi can be produced economically. Living nourishment organisms such as Artemia nauplii play an essential role in the larval rearing of barrramundi. However, zooplanktons in natural aquatic enviroments contain minerals in a higher concentration than the usually fed newly hatched Artemia. Therefore the goal of recent study was to investigate the effect of cobaltchloride on the larval growth and survival of barramundi when fed individually and combined with Zn and Mn supplemented Artemia.
In our experiment a 24 hours period was used for the enrichment of newly hatched Artemia nauplii with cobalt chloride in itself, as well as in combination with zinc sulphate and manganese chloride. A total of 1900 barramundi larvae from 15–30 day post hatching were fed with supplemented Artemia in 9 groups of treatments in duplicate. The growth performance and elemental concentration of 40 larvae from each group was determined. All the groups produced significantly improved growth compared to the control (p<0.05). The lower concentration of individual Co supplementation resulted in a higher growth performance while the opposite dose relation occured when combined the Co with Mn. Cobalt had a significant negative effect on the Mn uptake of the larvae – significantly higher Mn accumulation compared to the control group was only observed when Mn was fed in itself (p<0.05). In case of Co-Zn-1 and Co-Zn-2 treatments significantly higher Zn concentration was measured than in the others (p<0.05).
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Effects of combined nutrient supply treatments on some physiological parameters of autumn wheat
241-251Views:353The Fleischmann Rudolf Research Institute in Kompolt is not only famous for plant breeding but the institute also surveys the effects of different nutrient supply methods since 1918. In 2017, we joined this research supported by EFOP 3.6.1 project. Our aim was to investigate photochemical processes – which is one of the most determinant in case of yield – of crops by in vivo field measurements. We measured the chlorophyll content of leaves using Minolta SPAD 502. We used miniPAM fluorometer to determine actual photochemical efficiency and non-photochemical quenching of PSII during natural light conditions and also to evaluate the pigment (chlorophylls and carotenoids) and water content of leaves we applied field spectrophotometer (ASD FieldSpecPro 3). We utilized these methods by various treatments (1. treatment with soil bacteria + head and base fertilizer; 2. treated by only head fertilizer; 3. treated by only base-fertilizer) in field experiment of autumn wheat (4.1–2.43–1.19 ha) in June, 2017. The difference between treatments was clearly detectable. In the case of the first treatment, physiological processes were more intense and the ripening occurred earlier. The obtained yield was the highest in the case of the area treated by soil bacterial. Based on the results, the first treatment can be recommended in practice.
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Ecological Conditions of Agricultural Land Use in Transcarpathia
190-194Views:224The unbalanced anthropogenic effects for several decades resulted in significant technogen damages in the ecosystem of Ukraine. Excessive land development, including the use of slopes, effected the disintegration of the natural balance of lands – arable-lands, meadows, forests, and watershed areas – producing quite a negative effect on the landscape’s nature itself. It has to be stressed that according to other indexes, too, agricultural lands show a tendentious deterioration.
Erosion, caused by water and wind, is one of the most influential factors in the degradation of agricultural soils and in the reduction of the productiveness of benefital lands. Nowadays the degree erosion became significant and it directly endangers the existence of the soil which is a principal chain-link of the agricultural cultivation as well as an irreplaceable element of the biosphere.
The social and political changes in Ukraine’s life demand fundamental modernization in the land utilization both in ecological and in economical aspects. However, these aims can be realized only if, during the developments, we base on the up-to-date results of agronomics, and we do further research in the relations of agricultural land use and environmental protection. According to the latest theories, rational and environmental-safe agricultural production relates to the optimum correlation of the natural- and agricultural- ecosystems as well as to the reconstruction of agricultural areas built on the basis of environmental protection. -
Heat treatment and microbial digestion of poulty feather for biogas production
215-219Views:478The aim of this research was the elaboration of the technological parameters of heat pre-treatment and microbial digestion of poultry feathers for biogas production. Feathers were treated at 70, 100, 140 °C, and subsequently digested by Bacillus licheniformis, or keratin disintegrator bacteria. Investigations focused on the optimalization of parameters influencing poultry feather biodegradation. The optimal range of pH, temperature,
feather size and bacillus:feather ratio were determined in the experiments, as well as the analysis of relationship between the examined parameters. In order to be able to track the dynamics of the biodegradation, we determined the extinction level of the liquid phase of the biodegraded material in the different experimental treatments. The results showed that the rate of hydrolysis was significantly higher in the treatments with bacteria than in the treatments without it. Regarding the pretreatments at 70, 100 and 140 °C, the digestion of feather was the most intensive at 70 °C. The most extensive digestion was observed in case of 1:3 feather:water ratio. The highest intensity of feather digestion was
detected in the treatment with 1% microbe ratio. -
Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:522Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.
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Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:778Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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The effects of the extraction of non-ferrous metals on the ichthiofauna of the Lapus river bassin
6-8Views:184Over the period 2003-2005 we made ichthyologic research in the basin of the Lapus river. During the sampling we noticed that in some area the water was polluted. In those areas fishweretotallyorpartiallymissing.
The main pollution sources are those related to nonferrous metal extraction and processing, but there is also pollution from organic substances resulted from the communal residual waters, as well as the “tuica”(alcoholic drink) distilleries.
Confronting the spots of the pollution sources with the results of the ichthyologic research.we noticed a significantcorrespondencebetween the qualitative and quantitative component of the ichthyofauna and the presence of these sources.
We could, therefore prove the effect of the process of self-purificationofthewater,aswellastheexistenceofsomespeciesoffishshowing a great degree of tolerance towards pollution. -
Effect of cultivation factors on the yield and yield security of maize
263-265Views:405Cultivation factors have a significant effect on the yield and yield security of maize. Ensuring a suitable green crop is important. Tricultural crop rotation (pea–wheat–maize) in the average of 25 years provided a 2 t ha-1 higher yield compared to monocultural cultivation. A harmonious NPK nutriment supply determines yield and yield security, which can be especially realized by means of the application of precision cultivation technologies. Under average circumstances N 80 kg ha-1, P2O5 50 kg ha-1, K20 60 kg ha-1 active ingredient is the agro-ecological dosage of artificial fertilizer.
Plant density is a factor that determines yield. Optimal plant density – beside the genetic characteristics of the hybrid – is mostly influenced by the level of water and nutriment supply.