Search

Published After
Published Before

Search Results

  • Effect of Selenium Supplementation on in vitro Radish and Green Pepper Seedlings Germination
    149-155
    Views:
    184

    Selenium (Se) is an essential trace element for animals, microorganisms and some other eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although there is evidence that selenium is needed for the growth of algae, the question of essentiality of Se in vascular plants is unresolved. Therefore Raphanus sativus (Se accumulator) and Capsicum annuum (non Se accumulator) were treated with 0-200 mg/l natrium-selenate. The results showed that lower (2 mg/l) concentration natrium-selenate increased the fitomass and total antioxodant capacity in seedlings.

  • The effect of hybrid, nutrient-supply and irrigation on the grain moisture content at harvest and the starch-content of maize (Zea mays L.)
    89-95
    Views:
    116

    Maize is a worldwide dominant plant. According to nowadays plant production principles it is important to investigate and optimize the site-specific nutrient-supply and other production factors, such as hybrid and irrigation, in the case of this plant as well.
    At the Research Institute of the University of Debrecen, Center of Agricultural Sciences and Engineering, at Látókép the effect of nutrient-supply and irrigation on the quantity and quality parameters of different hybrids were investigated in a small plot long-term field experiment. In this paper we introduce the results regarding the corn moisture-content and the starch content of the yield.
    We have chosen three maize hybrids – that have been bread in Martonvásár – for our investigations. The effect of macronutrients is investigated in this experiment on five levels. The half of the experimental area can be irrigated during the vegetation period – whenever it is needed – by linear irrigation equipment, but on the other half only the water amount originating from the precipitation can be used by plants.
    In the year 2008 the hybrid affected the grain moisture content at P=0.1% level, while nutrient-supply had an effect at P=10% significance level. We haven’t revealed either any effect of irrigation or of interrelationship between production factors. It can be stated that there are differences between the hybrids on each nutrient-supply and on both irrigation levels. The grain moisture content increased parallel to the longer vegetation
    periods.
    The starch content of maize is mostly affected by the hybrid,
    so on P=0.1% significance level. Regarding our results, it can be
    stated, that the starch content shows a decreasing tendency
    parallel to the longer vegetation periods.

  • Q-PCR analysis of the resistance of Hungarian Botrytis cinerea isolates toward azoxystrobin
    41-44
    Views:
    113

    The genes being in the mitochondrial DNA primarily encode the enzymes of cellular respiration. Fungicides belonging to the family of quinol oxidase inhibitors (QoIs) play on important role in the protection against several plant diseases caused by fungi. These fungicides bind to the cytochrome bc1 complex so they block electron transport between cytochrome b and cytochrome c1. This way these fungicides inhibit the ATP synthesis consequently they inhibit the mitochondrial respiration. The QoI resistance has two mechanisms. One of them is the point mutation of the cytochrome b gene (CYTB), e.g. the substitution of a single glycine by alanine at position 143 results in high-resistance. The other is the cyanide-resistant alternative respiration sustained by the alternative oxidase.
    In a cell there are several mitochondria. The phenomenon when the genomes of all mitochondria in the cell are identical is called homoplazmy. If in the cell there is wild and mutant mitochondrial DNA this is called heteroplasmy. Whether the mutation in the mitochondria causes fenotypical diversity or does not depend on the dose, i.e. it depends on the percentage of the changed mitochondrials. During our work we investigated Botrytis cinerea single spore isolates which have been collected in 2008-2009 on different host plants. Our goal was to decide whether heteroplasmy influences the level of resistance. We managed to detect the change of the level of heteroplasmy, so the change the level of the resistance due to the treatment with fungicide.

  • The role of leaf area and photosynthesis in optimising the sowing date of maize
    5-10
    Views:
    139

     

    Our sowing date experiment took place in the Demonstration Garden of Institution of Plant Sciences, Agricultural Center of University of Debrecen, in 2012–2014. The thesis contains data of test year 2014. Our purpose, besides several other examinations, was to observe how sowing date influences leaf area index and activity of photosynthesis of maize hybrids, and how those factors affect fruiting. In the experiment we monitored the change of the leaf area index and the photosynthesis of hybrids with four different growing seasons.

    Based on the results, it can be concluded that most of the examined hybrids reached their smallest leaf area with the third sowing date and with the highest yield results. Hybrid Da Sonka had the largest leaf area (4.10 m2 m-2), and hybrid DKC 4590 produced the highest yield (13.16 t ha-1)

    with the third sowing date.

    During testing the photosynthetic capacity, the extremely high performance of the youngest plants with the third sowing date is outstanding, which can be explained by the different ripening periods. Examination of the correlation between the photosynthetic capacity and the yield, by linear regression analysis, also proves that photosynthesis has a determinative role in fruiting.

    The results obtained confirm that not only the environmental and agricultural factors in the growing season have effect on the yield, but also other factors like the leaf area index and the photosynthesis are determinative parameters, and all those factors together, modifying effects of each other, develop average yields.

  • Grain yield and quality of maize hybrids in different FAO maturity groups
    126-131
    Views:
    74

    An improvement in the quality of maize grain by increasing the level of components responsible for its biological value is possible
    by using genetic means. However, a change in the genotype, together with improving the nutrient properties of the grain, also has some
    adverse consequences connected with a fall in yield and in resistance to diseases.
    Field experiments were conducted during three years (2003, 2004and 2005) to evaluate environmental effects on grain yield and
    quality responses of maize hybrids. Twenty one hybrids of various maturity groups (FAO 150-400) were planted to achieve an optimum
    (60-70 000 plants per hectare) plant populations and grown under the medium-N (80 kg N ha-1) fertilization. Environmental conditions
    significantly affected maize hybrid responses for grain yield, starch, oil and protein contents, and consequently, starch, oil and protein
    yields per hectare. Hybrids of flint type, which have a short vegetation period, had high protein and oil content but the yield averages
    were low due to the slower rate of starch incorporation. Hybrids of the dent type have a longer growing season and more intense
    carbohydrate accumulation, but low protein and oil contents. In wet years there was a higher rate of starch accumulation, while dry
    years are favorable for protein and oil accumulation. Positive correlation existed between starch content and grain yield and 1000-
    weight as well as between oil content and volumetric weight among tested hybrids. Negatively correlation existed between grain oil and
    starch content as well as between oil content and grain yield and 1000-weight. Thus, end-users that require high quality maize may need
    to provide incentives to growers to off set the negative correlation of grain yield with oil and protein content.

  • Timing it right: The Measurement and Prediction of Flowering
    17-22
    Views:
    73

    Although the model described here was developed from research in controlled environments, there is now considerable evidence that in can be applied to a very wide range of natural environments in several species. Multi-locational trials augmented by successional sowing and, if considered necessary, supplementary illumination in the field to increase daylength, can be used to estimate the values of the model coefficients: (1) to characterize germplasm collections and so predict flowering behaviour elsewhere; (2) for interpreting and understanding crop adaptation; and (3) for genetic analysis of photoperiod sensitivity. We do not yet know whether the model has any contribution to make to the understanding of the biochemical mechanisms of photoperiod and temperature responses, but at the very least, it should provide the basis for indicating the most appropriate environmental conditions, genotypes and physiological stage of the plants most suitable for such investigations.

  • Experiences of Waste Management at Agricultural Enterprises in Hajdú-Bihar County
    50-55
    Views:
    74

    The general tendency of the last few years has been that besides large agricultural Ltd.’s and firms, smaller agricultural enterprises also have to correspond to stricter environmental regulations.
    This process suits the EU’s environmental and waste management regulations. Recently, manure, liquid manure and addle are registered as non hazardous agricultural waste in waste management in Hungary.
    In the case of the amount of these wastes is larger than 200 t/year, one has to make an individual waste management plan.
    Accordingly, several small agricultural enterprises have to make individual waste management plants in Hajdú-Bihar County.
    This article is based on the results of these companies experiences.

  • Preface
    5
    Views:
    36

    In the frame of a common “Hungarian-Ukrainian Intergovernmental S&T Cooperation Programme” which title is “Change of soils ecological characteristics of Ukraine and Hungary in the conditions of anthropogenic transformed ecosystems and optimization of biological processes of plants primary feeds elements mobilization” a Workshop was held in Debrecen. The member institutes of project participated with different presentation in this program.
    The title of Workshop was: “Anthropogenic effect on the properties of Middle and Eastern European chernozem soils and on the sustainable agricultural production”.
    The aim of the Workshop was to give relevant information about the present situation of the Middle and Eastern European Chernozem soils, especially emphasize the effect of different loading on the quality (properties) of chernozem soils. With the Workshop we would like to create a tradition for discussion about the anthropogenic effect on the soil properties and through it on the productivity of different soils. It was a forum for discussion of research results related to problems and possibilities for prevention of soil quality. With this possibility we would like to contribute to the sustainable agricultural production.
    The papers were read for the publisher and we would like to show them in a separate supplement of Journal of Agricultural Sciences, Acta Agraria Debreceniensis as one of the results of the project.
    The papers comply with the requirements of the scientific issue except those two which show the university and the department of the Ukrainian partner taking part in this project.
    The participant Institutes of the project:
    - Dnepropetrovsk National University, Faculty of Biology and Ecology;
    - Kryvyi Rig Botanical Garden NAS of Ukraine, Plant Physiology & Soil Biology Department;
    - Department of Agrochemistry and Soil Sciences of Centre for Agricultural and Applied Economics;
    - Research Institute of Karcag, Centre for Agricultural and Applied Economics. The collaboration with Ukrainian partners was successful and we have confidence in the further cooperation in scientific research.

  • Element content analyses in the Institute for Food Sciences, Quality Assurance and Microbiology
    203-207
    Views:
    130

    The role of chemical elements to ensure and promote our health is undisputed. Some of them are essential for plants, animals and human, others can cause diseases. The major source of mineral constituents is food, drinking water has a minor contribution to it, so the knowledge of elemental intake through food is crucial and needs continuous monitoring and by this way it promotes the food quality assurance and dietetics.
    With the evolution of spectroscopic methods increasingly lower concentrations could be determined, so the elemental composition of a sample could be more precisely and fully described. Due to the results the gathered knowledge up to the present is supported and new observations can be done helping us to understand such complex systems as biological organisms are.
    The quality of a food is determined by the full process of its production, consequently it starts with agricultural production so elemental-analysis usually cover the whole soil – plant – (animal) – food chain, by this way the „Fork-to-Farm” precept is true in elemental analysis field also.
    The history of elemental analysis in the University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Food Processing, Quality Assurance and Microbiology goes back to 1980s when the so called Regional Measurement Central gave the background for research. The continuous deployment resulted in an obtain of an inductively coupled plasma atomic emission spectrometer (ICP-AES) in 1988, which extended the scope of examinations due to its excellent performance characteristics
    compared to flame atom absorption (FAAS) and flame emission spectrometers (FES). The instrumental park retain up to date correlate to the developing analytical techniques due to acquiring a newer ICPAES in 1998 and an inductively coupled plasma mass spectrometer in 2004 – which sensitivity is three order of magnitude better compared to ICP-AES. The Institute supports the work with its own ICP-AES and ICP-MS since 2011. 

  • Examination of lead absorption ability on chernozem soil and the observation of the accumulation effect of Lactusa sativa L. in pot experimentation
    101-104
    Views:
    146

    In our research a chernozem soil sample formed on loess was collected from an area under cultivation. Our aim was to determine the lead adsorption capacity using a soil column experiment. The study showed saturation of lead content of the soil. The lead accumulation capacity of Lactuca sativa L. was measured in the sections of roots and leaves applying pot experiments. It could be observed that the lettuce accumulated lead easily from the chernozem soil. The lead content was increased in the analyzed sections of the plants against an increasing lead content.

  • Basil (Ocimum basilicum L.) harvest and plant replacement methods in aquaponia
    91-98
    Views:
    97

    The aim of the study is to investigate the potential of basil leaf mass production under aquaponic conditions with different harvest and plant replacement methods. Aquaponics is a combination of soil-less crop production hydroponics and aquaculture and it is can use and clean the wastewater of intensive aquaculture systems. Three groups were established in the 6 units during the six-week harvest and seedling rotation cycles. Group 1 individuals remain in the units throughout the breeding season. Group 2 individuals were replaced every 12 weeks, while Group 3 individuals were replaced every six weeks, at the same time as harvest. Data from the experiment were analysed to determine how the harvest and replacement protocol of basil plants influences the amount of leaves harvested, the percentage of leaves harvested relative to the plant stem, and the changes in plant height, SPAD and NDVI during harvest and replacement. A continuously maintained and harvested healthy basil stock under aquaponic conditions can provide a consistent leaf mass all year round without the extra cost of replacing and producing seedlings.

  • The Effect of Zanthium Extracts on Germination in Relation to Interior and Exterior Factors
    65-69
    Views:
    93

    In Hungary, the cocklebur species significantly endanger row crops (i.e. corn, sunflower, sugar beet). Their fast spreading is caused by many reasons: long-lasting emergence, reduced sensitivity to many kinds of herbicides, vigorous competitional ability, allelopathy, fast initial growth and changes in climate. The means of allelopathy is used by many species of plants in the competition with one another, as well as by the cockleburs. But the efficiency of this is influenced by many factors. Species with allelopathical effect could show different behaviour under different conditions: they can produce compounds with retarding and stimulating effects in different quantities or compositions. The actual condition of the acceptor plant and the mediator agent (i.e. soil) influence the final effect.
    In this experiment influence of phenology and rainfall was studied on allelopathy of cockleburs in May, July and September. A stronger inhibition was observed on growth of cress before rain in July than in May, but this retarding effect disappeared after rainfall. However, the samples collected in September have significant inhibitory effect after rain. Inhibition of sugarbeet’s germination was stronger in May than in July, and the difference increased after rain.

  • The effect of copper fungicides on the rate of photosynthesis and the transpiration of hop plants
    117-123
    Views:
    107

    The paper evaluates the effect of copper fungicide spraying on the rate of photosynthesis and transpiration of hops, the influence of spraying on the elemental copper content in the leaves and cones hop variety Agnus. Photosynthetic rate was measured by LC pro+ (infrared analyzer) in the Hop Research Institute Saaz in the field in some periods of 2008, 2009 and 2010. Dry cones and leaf samples (taken before and after application of copper fungicides) were analyzed in an accredited laboratory for elemental copper.

  • Phytopatological properties of symbiotic Rhizoctonia solani strains associated to orchids
    65-71
    Views:
    155

    The mycobiota of the Orchidarium of ELTE Botanical Garden (Budapest) has been studied applying aerobiological methods and isolating of tissue samples taken from 92 individuals of sixty orchid species. Among isolated basidiomycetaceous fungi 13 strains of Rhizoctonia solani were surviving in axenic culture. These symbiotic R. solani strains proved to be pathogenic on 24 cultivated plant species at varying degree. The symptoms of disease caused by R. solani strains isolated from orchids did not differ from that caused by reference strains. Three groups of strains could be separated regardless of their source or aggressivity. The host plants clustered into two groups, and their taxonomic position had no role in this respect. In general, we can assume that orchid associated Rhizoctonia strains are potential plant pathogens, and removed or withdrawn orchid stools should be treated as hazardous waste.

  • Some strategic aspects of animal protein production
    11-19
    Views:
    191

    The access to food shaped human societies and dietary models throughout the history of mankind. Animal protein consumption became a part of human culture. Data are presented showing the relationship of daily calorie and animal protein consumption as affected by capita GDP changes. Examples are presented how genetic improvement of animal and fodder plants influenced the resource efficiency and the overall environmental footprint per unit product. The two examples presented are: the dairy industry of the USA the 1944 and 2007 situation, and the Hungarian broiler chicken sector considering data relevant to 1930, 1960 and 2010. In both cases, dramatic improvements in resource efficiency could be demonstrated. The agricultural area required to animal feed production was reduced by more than 80% in both cases per unit product. Future possibilities are briefly discussed, referring to the still unutilized land reserves of the Globe, the new evolving technologies in progress inclusive the CASPR/Cas 9 genetic editing methods.

  • The olive (Olea europaea) and the almond (Prunus amygdalus) related phytonutrients, and the associated health-promoting biological effects, a review
    11-24
    Views:
    246

    With the increasing attention to the health promoting activities of the bioactive compounds from some plants, many researchers are focusing on the biological potential and mechanisms of certain cultivated plant species. In this review, we survey the olive and almond based extracts specific phytoconstituents and their associated health promoting effects that have been evaluated in experimental and clinical studies.

     

  • Morphological and leaf anatomical studies on Cleistogenes Serotina (L.) Keng (Poaceae)
    5-13
    Views:
    90

    The grass species Cleistogenes serotina (L.) Keng, generally found in the mountainous region in Hungary, has been observed in the lowlands of the Great Hungarian Plain in the last few decades. Since its first recent mention in sand habitat as well it, has been found in several locations in the interfluve between the Danube and the Tisza. In certain, sandy grasslands, the invasion of this species is considerable (e.g. near Fülöpháza the area invaded by Cleistogenes increased almost fourfold within six years). The success of invasion on new habitats is related to changes in the
    characteristics of plants comparatively studied on mountain and sand populations.
    We studied blades of leaves on different levels (1st, 5th and 10th) and from different biotops. Cuttings were made, with a thickness of 10-12 μm, at the middle of the blades. The cross sections were observed using a light microscope. Digitised images (DigitPlan) and survey data were arranged into a database.
    The leaf structure is characterised by the kranz anatomy, and a double layered bundle sheath, which is typical of the aspartate type of C4 grasses. There were significant differences only in cross section area and the number of bundles for various ages of the leaves. There were more bundles in the older leaves than in the younger ones. We did not find any difference in the ratio of tissues among leaves of different ages and sources. According to our study, the ratio of leaf tissues characteristic to species was neither significantly influenced by the biotop, nor by the age of the leaves. However, there is a difference in tissues size. Summarising the results, the species can be considered „only” as ecotypes on the
    different studied areas. 
    Our new result is that kranz anatomy can also be found in the leaf-sheath.
    This research was supported by the Hungarian Scientific Fund (OTKA), grant No. T016511 and T026346.

  • The Effect of Atmosperical Aridity on the Changes of Quantitative Parameters of Horticultural and Arable Crops
    40-45
    Views:
    127

    The occurence of atmospherical drought causes serious water-supply problems in the most cases of our domestic agricultural plant species. This paper was studied, how can we quantificate the atmospherical drought, with the help of a low input (relative humidityof the air, temperature) index. If this index (LSZI) characterized the atmospherical drought well, it will suitable to estimate the yield amount of agricultural plants.
    The index elaborated by the authors was tested on county average crop yield of 14 agricultural plant species. Moreover we compared the atmospherical drought index (LSZI) to other aridity parameters, how suitable for estimate the yield amount.
    Result of experiments show that, the atmospherical drought index (LSZI) can be used well by several agricultural plant species in especially coern and sugar-beet to estimate yield amount. Excellent results were found by comparison to other aridity indexes, this means it is worth using in the aridity researches in the future.

  • Effects of soil cultivation and environmental changes on maize yield
    97-100
    Views:
    155

    We evaluated the relationships among soil cultivation and other agrotechnical factors (fertilization, number of plants and hybrid) within the framework of a multifactorial long-term experiment set at the Látókép Experimental Site of the Centre for Agricultural Sciences of the University of Debrecen in mid-heavy chalcareous chernozem soil based on a long-term experiment conducted for a 5-year period (2002–2006).

    Based on the evaluation of soil cultivation by the average of treatments, it may be assessed that spring ploughing (8.204 t ha-1) provides more favourable conditions to the stand compared to spring shallow cultivation; however, this did not result in a significant difference. Spring ploughing considerably increased the yield of hybrid FAO 300 in dry years, whereas it considerably increased the yield of hybrid FAO 400 in favourable crop years. A stand of 70 thousand stems/ha provided the higher yield result in both soil cultivation types. It was sufficient to use a fertilizer dose of 120 kg N ha-1 for economical production.

  • Performance of agricultural factors on yield of  sweet corn (Zea mays L. Saccharata ) - A review
    143-156
    Views:
    100

     Sweet corn producers and industries require more reliable cultivars which could be accomplished by hybrid breeding. However, progressive phenological growth may be affected by different factors. In this paper, we analyze the key factors that determine the growth and yield of sweet corn. Environmental factors such as temperature and photoperiod were strong determinants of dates of flowering and harvest which are often crucial to yield in diverse climates and agricultural systems, besides the country's pedological conditions, especially soil fertility, affected phenological development. The effectiveness of fertilization in improving sweet corn growth performance was significantly influenced by the soil characteristics, the water supply, the genotype, and the agrotechnological factors. Therefore, genetic improvement of hybrids should be incorporated into the climate and soil elements to stabilize sweet corn yields in various agroecosystems. Decisions made in the sowing period are very significant, as up to 30% of the obtained yield may depend on making the proper choice. Deviation from the optimum date (either early or late sowing) may decrease yield. When deciding about the sowing date of maize, one needs to consider climate, soil quality, geographical location, temperature, weed infestation, sowing seed quality, and the ripening time of the hybrid to be produced.

  • Heavy Metals in Agricultural Soils
    85-89
    Views:
    78

    The soil constitutes the basis of the food chain. To keep soil conditions in a good trim is very important, it’s part of the sustainable development and of producing food supply harmless to health.
    In some cases, soil productivity is the only important part, qualitative requirements or economical characteristics can improve it. The soil is threatened by two danger factors: the soil degradation and the soil pollution. The accumulation of different harmful and/or toxic substances in the soil is well known. Heavy metals constitute a part of it. Metals in the soil and in the soil-solution are balanced. This balance depends on the type of the metal, on the pH, on the cation-band capacity of the soil, on the redox relations and the concentration of cations in the soil.
    To be able to handle the metal contamination of the soil, it is important to estimate the form, the possible extension and the concentration of metals.
    Of course, the different types of soils have different physical-chemical, biological and buffer capacity, they can moderate or reinforce the harmful effects of heavy metals. To draw general conclusion of the dispersion and quantitative relations on the metals originated from different contamination sources is hard, because in some emissive sources contamination is limited in small areas but on a high level, some others usually expand on larger areas, and as a result of equal dispersion, the contamination’s level is lower.
    Heavy metals – unlike alkali ions – strongly bond to organic materials, or infiltrate in a kelát form. Their outstanding characteristic is the tendency to create metal-complex forms. Kelats take part in the uptaking and transportation of heavy metals. Heavy metals exert their effects mostly as enzyme-activators.
    The metals cannot degrade in an organic way, they accumulate in living organisms, and they can form toxic compounds through biochemical reactions.
    Lot of the heavy metals accumulate on the boundaries of the abiotic systems (air/soil, water/sediment), when physical or chemical parameters change, and this influences their remobilization.
    Human activity plays a great part in heavy metal mobilization, results in the human origin of most biochemical process of metals.
    To understand the toxic influence of accumulated metals of high concentration, their transportation from soils to plants or their damage in human health, must clearly defined and investigated.
    For effective protection against soil pollution, the types and levels of harmful pollution to soil must identified, regarding legal, technical and soil-science aspects, preferable in a single way. Difficulties in this area mean that toxicity depends on loading, uptake, soil characteristics and living organisms (species, age, condition etc.), furthermore, local and economic conditions considerably differ.

  • Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
    34-39
    Views:
    142

    Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
    The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
    The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested.

  • Effect of soil-compost proportion on the abiotic and biotic parameters of soilplant system
    99-104
    Views:
    111

    The environmental awareness, coming to the front in the 21st century, motivates us to supply the plant nutrient demand (in point of the plant, the environment and the human health) with natural materials.
    Composting is known since the beginning of civilization. We came to know more the processes of composting as a result of last decades’ research, but numerous unexplained questions remained up to this day. The good compost is dark gray or brown, and it should not create an odor. It has aggregate structure, and it’s pH is neutral. Compost is soil-like (Fehér, 2001), nutrient-rich material, which contains valuable nutrients extracted from soil, so if we recycle this, we can decrease the chemical fertilizer and other (example: mineral energy) expenses.
    The reason of that we chose the more accurate cognition of compost utilization is to do more effective the site-specific nutrient supply. This increases the average yield and the quality of yield. Besides we can decrease the harmful effects, which endanger the plant, the environment, and the human body.
    During the compost utilization experiment we blended the  acid sandy soil with compost in 4 different volumetric proportions (5 treatments) than we set the pots randomized. The advantage of this method is that we can provide equal conditions for plants so we can measure the effect of  treatments correctly. Our experimental plant was ryegrass (Lolium perenne L.), that grows rapidly, tolerates the glasshouse conditions, and indicates the effect of treatments well. After the harvest of ryegrass we measured the fresh and dry weight of harvested leaves and the total C-, N-, S-content of the dry matter and of the soil, we examined the pH and the salt concentration of  soil as well. 
    Our aim was to study and evaluate the relations between the compost-soil proportion and the nutrient content of soil and plant. In our previous experiments we confirmed (based on variance analyses) that the compost has a beneficial effect on soil and increases the nutrient content of the soil (Szabó, 2009). But it’s important to appoint that the compound of compost is seasonally change: in winter the selective gathered municipal solid waste contains salt that were applied for non-skidding of roads, but salt has a negative effect to the plant. We proved that in our experiment the 25/75% compost/soil proportion was ideal for the plant. This content of compost effected 6 times higher green matter weight compared to the 100% sandy soil. 

  • In vitro analysis of the effect of ragweed extract against Monilinia laxa
    117-120
    Views:
    119

    Nowadays in Hungary nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia). According to the public opinion the ragweed is a weed. From agricultural and public health point of view it is exceptionally dangerous plant. As it contains a number of useful active ingredients, based on this the  ragweed is consider a medicinal plant. Our goal was to present that the ragweed contains antifungal active substances as well. In the experiments we used the pre-flowering plants with roots and we extracted the biological active components of dried plant. We tested the biological activity of the extracts against Monilinia laxa in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Monilinia laxa.

  • Varietal dependent response of barley to soil-borne Waitea circinata infection
    100-106
    Views:
    169

    The disease syndrome caused by Waitea circinata, a soil-borne pathogen introduced in the past decade into Carpathian basin, visually indistinguishable of those caused by various Rhizoctonia strains in diverse host plant. Dicotyledonaceous species in general proved to be more tolerant to this new pathogen than monotyledonaceous ones. This mesophilic fungus can seriously damage cereals. The barley varieties, similarly to other plants, exhibited highly different individual reaction to soil borne infection, Bivoy being the most while Maresi the less tolerant among the 9 tested varieties. Two groups could be separated on the base of their response to Rhizoctonia; Jubilant, Bivoy, Pasadena formed one group being moderately tolerant and Anabell, Scarlett, Rex and Omega the other group of more susceptibles. Three significant factors influence on the virulence of Rhizoctonia strains comprised 62% of total variation.