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The role of non-optimum Fe-Zn ratio in the development of latent zinc shortage in cucumber (Cucumis sativus L.)
7-11Views:126The general micronutrient deficiency of the soils influences the quality of food production which causes human health problems in several countries as well. The non optimal Fe-Zn ratio can cause latent zinc deficiency – which the plants response in the function of their sensitivity –what has no visual symptoms or the plant shows deficiency symptoms in case of appropriate zinc supply. This phenomenon can cause significant decrease in the crop yield.
The aim of this study was to prove the role of non optimal Fe-Zn ratio in the evalution of latent zinc deficiency.
The non optimal Fe-Zn ratio caused decrease in the number of the leaves, the number and length of the internodes, the relative chlorophyll contents and in the dry matter production. According to the results the non optimal Fe-Zn ratio caused difficulties in the metabolism, which decreased the examined plant physiological parameters in the most cases. It can be concluded if there are higher iron contents in the tissues than zinc it can result latent zinc deficiency.
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Population genetic results of Hungarian Botrytis cinerea isolates establishing new technologies with decreased chemical usage against grey mould
259-261Views:103Botrytis cinerea causes gray mold on a high number of crop plants. Information about the populations of plant pathogen fungi may help to develop new strategies for the effective and economic crop protection with reduced fungicide usage. Hungarian B. cinerea populations were characterized with using different molecular genetic parameters. B. cinerea group I strains, characterized with high rate of fenhexamid resistance, could be detected only in restricted number. The Hungarian B. cinerea populations were characterized with high genetic diversity, and the regular occurrence of sexual reproduction. These results highlight the importance of rotating different type of fungicide in the plant protection technology against grey mould.
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Using research findings in precision maize production
227-231Views:195The effect of crop production factors on maize yield are examined on chernozem soil in a more than 30 year old long-term experiment on the Látókép Experiment Site of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen. The aim of research is to evaluate the effect of fertilisation, cultivation, plant number, genorype and irrigation. The analysis of the data in the database of the examined period makes it possible to evaluate the effect of maize yield, as well as that of the crop production factors and the crop year, while the correlations and interactions between these factors were also examined. During the examination of the cultivation treatments, it was concluded that the highest yield was obtained as a result of autumn ploughing, but its effect largely differs in the irrigated and the nonirrigated treatments. Based on our examinations, strip cultivation should be applied periodically (e.g. strip – strip – ploughing – loosening) in areas with favourable soil conditions free from compacted layers.
In years with smaller, average precipitation supply or when the precipitation was higher than average, higher plant numbers were more favourable. Under drier conditions, but especially in several consecutively dry years, a lower plant number can be recommended which is not higher than 60 thousand per hectare. In the case of favourable water supply, 70-80 thousand plants per hectare can be used. The yield increasing effect of fertilisation was significant in the case of both non-irrigated and irrigated conditions, but it was much more moderate in the non-irrigated treatment. The extent of weed coverage was significantly affected by the previous crop. In the case of a favourable previous crop (wheat), the weed coverage was significantly lower than after an unfavourable previous crop (maize). In the case of the same previous crop (maize), the extent of weed coverage was mostly determined by the crop year and the extent of precipitation supply. Irrigation is not enough in itself, because if it was not accompanied by intensive nutrient management, yields started to decline.
The results of researhc, development and innovation contributed to the technological method which makes it possible to apply locally adjusted sowing seed, fertiliser and pesticide in a differentiated way, as well as to change the method of operations within the given plot. -
Slight damage of the great green bush-cricket (Tettigonia viridissima) (Orthoptera: Tettigoniidae) in some Hungarian maize fields
65-70Views:215Characteristic cricket damage was observed in two maize fields in northern Hungary, at Máriabesnyő, a district of Gödöllő. The damage level of the two fields did not differ significantly and continual monitoring of field1 showed also a stable infestation level. T. viridissima nymphs and a female were found and observed as feeding on maize plants. The crickets must have disappeared after 18.07. because no more fresh damage was observed after this date. The chewing’s number about on one and two % of the examined plants amounted one and six a plant and their size was between one and eight cm2. This infestation was quite little and might have caused apparently no yield loss. Compared this damage of T. viridissima with former Hungarian experience, this was the usual negligible damage despite the explicit draught in July and August 2015. As regards the global warming, orthopteran damage may be more obvious in the future.
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The influence of crop rotation and nutrition regime in winter wheat on the phytomass remains and the deposits of weed seeds remained in the ground
10-13Views:127An important problem refers to soil weeds seed bank with special reference to brown luvic soils and relationship between vegetal remains
(roots + stub) and nutrition regime – crop rotation plant. A great number of seeds can be found in first 20 centimeters of soil (plowing
layer). This fact causes a high weeds density, which has a negative effect on the cultivated plants leading to partial or total crop loss. -
Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil
111-115Views:185We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.
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Effects of soil cultivation and environmental changes on maize yield
97-100Views:126We evaluated the relationships among soil cultivation and other agrotechnical factors (fertilization, number of plants and hybrid) within the framework of a multifactorial long-term experiment set at the Látókép Experimental Site of the Centre for Agricultural Sciences of the University of Debrecen in mid-heavy chalcareous chernozem soil based on a long-term experiment conducted for a 5-year period (2002–2006).
Based on the evaluation of soil cultivation by the average of treatments, it may be assessed that spring ploughing (8.204 t ha-1) provides more favourable conditions to the stand compared to spring shallow cultivation; however, this did not result in a significant difference. Spring ploughing considerably increased the yield of hybrid FAO 300 in dry years, whereas it considerably increased the yield of hybrid FAO 400 in favourable crop years. A stand of 70 thousand stems/ha provided the higher yield result in both soil cultivation types. It was sufficient to use a fertilizer dose of 120 kg N ha-1 for economical production.
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Gene resources for improvement of drought tolerance and yield quality in dry pea breeding
105-111Views:86The effectiveness of selection for improved drought tolerance and consumption quality in the progeny of crosses between pea cultivars with semi-leafless (afila) and normal leaves and different origins, respectively, were investigated. After single crosses, parent cultivars and F1, F2 and F3 generations were grown under non-irrigated conditions in the same trials. We created a colour scale from 1 to 9 to measure statistically the shade of seed colour. The tolerance of genotypes against high temperature was measured by the number of pods per plant. The 3:1 segregation
observed in the F2 generation of crosses between semi-leafless and conventional cultivars indicated that the semi-leafless character is determined by a recessive gene. In contrast, the ratios of conventional (Af) and semi-leafless (af) genotypes were 7:1 and 9:1 ratio in the progenies of crosses of Af × af. The genetic progress was effective for improving the seed quality in F3 generation from crosses Af x af where we found that multiple
dominant alleles controlled the orange colour of cotyledons and its high heritability (h2 A=0,63). Selection is more effective in producing the genotypes with high yield and normal leaves if the crosses were made between the western European cultivars such as semi-leafless Profi and Delta used as maternal cultivars and conventional Auralia cultivars. In this case, there were decreases in the consumption quality, such as seed size and shade of colour. The selection based on the seed weight of single plants for increasing drought tolerance seemed to be more effective in F4
strains with normal leaves originated from Czechoslovakian maternal cultivar Y228; however, the genetic progress in the improvement of seed size and colour quality was slow. -
Possibilities of Weed Beet (Beta vulgaris, L. ssp. marritima) Regulation
133-136Views:87Weed infestation regulation in sugar beet belongs to the most difficult growing measures, the aim of our work was to find out, if active substances contained in preparations Roundup and Fazor influence number, weight and germination of clusters in plants of weed beets. Statistically significant differences in germinability were found among control and all variants in which preparation Fazor was applied. The Roundup treatment did not have significant influence on germination. Presented results are only preliminary and one-year results, more experiments are necessary to determine the effects of the studied applications.
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Pest species of Macrolepidoptera in the Game Reserve of Velyka Dobron’ (Transcarpathia, Ukraine)
58-64Views:213The Game Reserve of Nagydobrony extends on a marginal area of a former peatland and is covered with extended hardwood gallery forests and oak-hornbeam forests and is surrounded by a mosaic-like agricultural landscape. Due to its richness of nature-like and semi-natural habitats it supports a diverse insect assemblage. By light and bait trapping 383 species of macro-moths were recorded from which larvae of 85 species are feeding either on forest trees and scrubs or on cultivated plants thus these can be considered as potential pest species. Thirteen species (mostly Geometridae and Erebidae: Lymantriinae) have a special significance for forestry due to defoliating activity in gradation periods. Considering the habitat connections, the composition of moth assemblage is dominated by generalist species with broad spectrum of ecological tolerance but the species connected with humid forested habitats are also richly represented. The bulk of species consists of widely distributed Euro-Siberian species, but also some Holo-Mediterranean species with more southern character and Mediterranean-Subtropical migrant species were registered. The bait trapping provided significant results on the phenology of the dominant species. The faunistically significant and/or protected species were observed in a low number of individuals only, thus the applied trapping methods did not damage the faunal composition.
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Comparative study of honeys and herbhoneys quality paramertes
117-120Views:172Honey is one of the oldest known and consumed food. Besides the pleasant sweet taste it has been consumed because of the favorable health benefits. In addition to large number of honey plants there are many popular herbals which cannot serve as a nectar source. The herbhoney has been developed that consumers can joy the pleasant properties of herbals and honeys positive impact on the same product. In our research we compare the quality parameters of honeys and herbhoneys to each other and with the relevant regulation.
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The effect of the plant density for the yield of the maize hybrids
50-61Views:87In order to enchance the yield stability of maize, the effect of plant density on yields was studied on a typical meadow soil in Hajdúböszörmény between 2002-2004. In the plant density experiment, we used the method of Béla Győrffy. The plant densities applied therefore 20 to 100 thousand plants/ha by ten thousand scale. The application of fertilizer rates for the maize hibrids in every year were N: 110 P: 90 K: 120 kg/ha. We used a manual soiling-gun in the experiment. In every year we used plant protection techniques against monocotyledonous and dicotyledonous weeds. The harvest was done by hand. The facts were read by variancie analysis and linear regression analysis. The moisture and the temperatures were extreme in 2002, 2003, 2004. We have to mention defficiery of moisture in 2003 which is shown that the hot days number increased. After evaluating our findings we can conclude that most hybrids showed a significant correlation between increased plant density and the volume of yields. On the basis of the experiments we divided the hybrids into four groups: the first group included the hybrids suitable for increased plant density with a wide range of optimal density values; the second group included hybrids, which did not require high plant density, were capable of good individual performance and tended to grow several ears; the third group included flexible corn types, which grew longer ears in favourable years, thus yielded more; and the fourth group included the hybrids, which were sensitive to increased plant density and which showed a narrow range of optimal density values. Finally, plant density determines the yield; we have to consider optimal plant density intervals as well as optimal plant density, and we also have to place a high emphasis on the use of hybrid-specific technologies.
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Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:146Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
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Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:83We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:130Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Morphological and leaf anatomical studies on Cleistogenes Serotina (L.) Keng (Poaceae)
5-13Views:76The grass species Cleistogenes serotina (L.) Keng, generally found in the mountainous region in Hungary, has been observed in the lowlands of the Great Hungarian Plain in the last few decades. Since its first recent mention in sand habitat as well it, has been found in several locations in the interfluve between the Danube and the Tisza. In certain, sandy grasslands, the invasion of this species is considerable (e.g. near Fülöpháza the area invaded by Cleistogenes increased almost fourfold within six years). The success of invasion on new habitats is related to changes in the
characteristics of plants comparatively studied on mountain and sand populations.
We studied blades of leaves on different levels (1st, 5th and 10th) and from different biotops. Cuttings were made, with a thickness of 10-12 μm, at the middle of the blades. The cross sections were observed using a light microscope. Digitised images (DigitPlan) and survey data were arranged into a database.
The leaf structure is characterised by the kranz anatomy, and a double layered bundle sheath, which is typical of the aspartate type of C4 grasses. There were significant differences only in cross section area and the number of bundles for various ages of the leaves. There were more bundles in the older leaves than in the younger ones. We did not find any difference in the ratio of tissues among leaves of different ages and sources. According to our study, the ratio of leaf tissues characteristic to species was neither significantly influenced by the biotop, nor by the age of the leaves. However, there is a difference in tissues size. Summarising the results, the species can be considered „only” as ecotypes on the
different studied areas.
Our new result is that kranz anatomy can also be found in the leaf-sheath.
This research was supported by the Hungarian Scientific Fund (OTKA), grant No. T016511 and T026346. -
In vitro analysis of the effect of ragweed extract against Monilinia laxa
117-120Views:103Nowadays in Hungary nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia). According to the public opinion the ragweed is a weed. From agricultural and public health point of view it is exceptionally dangerous plant. As it contains a number of useful active ingredients, based on this the ragweed is consider a medicinal plant. Our goal was to present that the ragweed contains antifungal active substances as well. In the experiments we used the pre-flowering plants with roots and we extracted the biological active components of dried plant. We tested the biological activity of the extracts against Monilinia laxa in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Monilinia laxa.