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Genetic progress in winter wheat quality and quantity parameters
71-75Views:173Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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The role and impact of N-Lock (N-stabilizer) to the utilization of N in the main arable crops
51-55Views:236The nitrogen stabilizer called N-Lock can be used primarily with solid and liquid urea, UAN and other liquid nitrogen, slurry and manure. In corn it can be applied incorporated before sowing or with row-cultivator or applied with postemergent timing in tank-mix. In postemergent timing need precipitation for long effect. In oil seed rape and autumn cereals the N-Lock should be applied with liquid nitrogen in tank mix late winter or early spring (February-March). The dose rate is 2.5 l/ha. N-Lock increases the yield of maize, winter oil seed rape, winter wheat and winter barley 5-20 %. The yield increasing can be given the thousand grain weight. In case of high doses of nitrogen it can be observed higher yield. The quality parameter also improved, especially the oil content of winter oil seed rape and protein and gluten contents of winter wheat. The use of N-Lock increases the nitrogen retention of soil and reduces nitrate leaching towards the groundwater and the greenhouse effect gas emissions into the atmosphere. The degradation of the applied nitrogen is slowing down and the plant can uptake more nitrogen in long period. The effect of N-Lock the nitrogen is located in the upper soil layer of 0-30 cm and increasing the ammonium nitrogen form. The product can be mixed with herbicide products in main arable crops.
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The Examination of the Year Effect Based on Cumulative Temperature and Rainfall Curve
70-77Views:93In order to get to know the numerical differencies of favourable and unfavourable weather conditions, our research lean to 29-years-data (1961-1989) of maize yield of productive area of Hajdúság from which we chose two low (1964, 1976) and two high (1969, 1975) yield yerars. Choosing the years we fitted a linear trend to the time series and the valued data was divided by the realised crop. From the cumlative temperature- and rainfall data calculatd curve concern the period between 01. April and 01. September. The basis was the cumulative value of many years daily average value. The temperature- and rainfall curve of favourable and unfavourable yield with the many years average data was represented on the same scale. In order to numericate the differencies we count the differencies monthly frequency. Based on it became sunder the feature of years. The method used by us ensure possibility to separate the temporal types. It was found that informing the yield the rainfall has main role in this productive area and the temperature has only second role.
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Studying the effects of traits in the genotype of three maize hybrids in Hungary
97-101Views:370In this study, we selected three hybrids (Armagnac, Loupiac, and Sushi) for evaluation of the effect of traits in genotype in Debrecen. In 2017, the total rainfall from May to October was 314 mm in Debrecen, which was 236 mm in the winter period before sowing. The obtained results showed that there was a positive correlation between the weight of the cob maize and the rate of seed/cob, number of rows with number of seeds in column and outer diameter ear with weight of cob and number of rows in grain per ear and the rate of seed / cob; Also, there was a negative correlation between grain weight in ear with seed/cob rate, outer diameter ear and the rate of seed/cob and outer diameter ear with the number of leaves. There was a positive correlation between stem diameter, Seed/cob rate and the number of nodes by GGE biplot. In addition, there are traits of weight of all seeds and outer ear diameter that had the highest effect on average yield. Moreover, the number of seeds per row showed the least effect on the average yield of hybrids.
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The role of Debrecen maize hybrids in competitive production
19-21Views:76I examined the effects of plant density and fertilisation on the yield of the maize hybrid Debreceni 377 SC, developed by the Agrárgazdaság Ltd., using the 3 year data sequence of a long term experiment set up at the Látókép experimental station of DU Center for Agricultural Sciences in years with average precipitation supply.
Based on the evaluation of my research results, I found that the application of higher plant densities was more favourable in years with average or higher than average precipitation supply. During the examination of fertilisation effects, it was verified that surplus yield was realised in most cases where smaller fertilisation dosage, 120 kg per hectare active substance was applied. -
Comparative analysis of maize weed control system and the competitive effect of sorghum
97-104Views:110In our investigation we used different weed control technologies in the different phenology states of the maize. The experiment have been
carried out in Hódmezővásárhely, in the Experiment garden of the Pilot farm of University of Szeged Faculty of Agriculture, on meadow
chernozem soil, on 24 m2 plots, in 3 replications, randomized blotch design. The experiment can be regarded as 15 weed-control strategies
where, in addition to the untreated control, six chemicals or chemical-combinations are applied (Spectrum 720 EC, Motivell Turbo D,
Stellar + Dash HC, Clio + Akris SE + Dash HC, Clio + Dash HC) in five different times (pre, early post, post and two late post) and eight
mechanical weed-control technologies were used. Hoeing took place connected to the herbicide treatments in different times: until 2-3-leave
age weedless, in 3-4-leave age hoed once, from 3-4-leave age weedless, in 6-7-leave age hoed once, from 6-7-leave age weedless, in 8-leave
age hoed once, from 8-leave age weedless.
Our results were assessed by chemical efficiency examination, maize length measurement, corncob-length and fertility examination,
Sorghum plant-number determination and yield weighing carried out in four periods. The data were evaluated by a one-factor analysis of
variance and a two-factor linear regression analysis. -
Slight damage of the great green bush-cricket (Tettigonia viridissima) (Orthoptera: Tettigoniidae) in some Hungarian maize fields
65-70Views:245Characteristic cricket damage was observed in two maize fields in northern Hungary, at Máriabesnyő, a district of Gödöllő. The damage level of the two fields did not differ significantly and continual monitoring of field1 showed also a stable infestation level. T. viridissima nymphs and a female were found and observed as feeding on maize plants. The crickets must have disappeared after 18.07. because no more fresh damage was observed after this date. The chewing’s number about on one and two % of the examined plants amounted one and six a plant and their size was between one and eight cm2. This infestation was quite little and might have caused apparently no yield loss. Compared this damage of T. viridissima with former Hungarian experience, this was the usual negligible damage despite the explicit draught in July and August 2015. As regards the global warming, orthopteran damage may be more obvious in the future.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:252Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Evaluation of decreasing moisture content of different maize genotypes
147-151Views:293An experiment was conducted to evaluate the decrease in grain moisture content in three maize hybrids in Debrecen in 2017. Armagnac, Sushi and Loupiac were the examined hybrids in this study. The culture medium and temperature conditions were applied uniformly for all three hybrids. According to the results obtained from the ratio of moisture content of seeds per day, the Armagnac variety in the intensive drying down phase loses more time and moisture content, so it can be concluded that the produced dry matter is more than in the case of other varieties. Armagnac requires more time to achieve yield, while the Sushi and Loupiac hybrids produce less harvest. Regarding the slope of the regression line, the rate of loss of moisture in the grain has been negatively correlated with the amount of “b” in the three examined hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97% in the Loupiac, 95% and in the Sushi 90% of the total dynamic value of moisture motion.
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Impact of precision irrigation on the unit income of maize production
157-162Views:80The study of the economic/economic impact of precision farming should be a priority area in digital agriculture, as the results, profitability, and efficiency indicators can have a significant decision-support effect on the development of both the agronomic and the technical regions of individual farms both in the longer and shorter term. Individual firms, companies, farmers, and family farms quantify the effectiveness of their farming processes. The modern age offers the possibility of digitally recording all the elements of farming technology, making it possible to analyse the cost-effectiveness of a farm more effectively and, in some cases, to carry out more detailed analyses. Nevertheless, the number of farms demonstrating their profitability with such precise economic calculations is still minimal.
Our analyses were conducted on a 56,02 ha field of Balogh Farm-Tépe Ltd. The agricultural operations carried out were fully documented so that the inputs (seeds, fertilisers, pesticides, crop enhancers) were recorded in coordinates and kind, as well as the specific yields, grain moisture data, irrigation norms, and irrigation rotations. At the same time, the company's owner provided the data's monetary value. The main econometric indicators (yield, production value, cost of production, income, cost price) related to the evaluation of the enterprise management were evaluated along with the spatial data in the irrigated and non-irrigated tables. Our calculations show that a given year's climatic and market characteristics fundamentally determine the cost and income relations of a plot of land (and thus of an entire farm). In addition to additional inputs, introducing some elements of precision farming and intensification and increasing yields improves yield security and allows for excellent yield stability.
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Evalution of energy for bioethanol production
77-80Views:135The objective of this study was the ethanol which classified as agro fuels. The aim of our research was the calculation of efficiency of bioethanol production, and evaluates the yield of maize hybrids grown for this purpose. We examined the energy demand of corn production per hectare in two vintages of 2009 and 2010. The focus of the experiment was placed in three different doze of fertilizer. Results show that the control corn plot used the least amount of non-renewable energy. Improving starch yield by adding fertilizer required additional nonrenewable energy inputs. So then the invested energy has a great impact on the efficiency.
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Effect of extreme crop year on soil moisture in maize
35-40Views:84We examined the change of the time of water balance of soil in 25 years old experiment, on chernozem soil, in different croprotation systems (mono-, bi- and triculture) in two extreme cropyear in 2007 and 2009 in maizestock.
According to our findings the values of waterdeficit of soil of maizestock were about 100 mm before the sowing time that grew because of considerable deficit of precipitation and high average temperature in months of summer. Values of waterdeficit achieved at the end of August the maximum and lessed a little bit to end of crop time. Decrease of waterstock stopped because of irrigation treatments in irrigated plots but the difference between two irrigation treatments (Ö1-Ö3) vanishedat the end of summer, waterdeficit were higher with 17 mm in monoculture in irrigated plot than value of not-irrigated plot. Considerabler precipitation in Jun effected on waterbalance of soils of three of crop-rotation systems favourable, rapid waterloss starting to april began to lessenat the end of May and started to increase from early in July. Precipitation in Jun had positiv effect on yield also. -
The effect of NPK fertilization and the plant density on maize yield and bioethanol production
13-18Views:126For industrial (bioethanol) production of maize, a new production technology is needed. I tested and selected hybrids appropriate for this purpose and set up fertilization and plant density experiments. The experiment were set up on chernozem soil in 2008.
In bioethanol production, the selection of a high-yielding hybrid with high starch content, a slight reduction of N, increase of potassium, the application of the highest plant densities of the optimum interval, harvest at full maturity (when starch content is the highest compared to protein content) are of great importance. -
The Effect of Drought on the Yield of Winter Barley Lines in the Great Cumania Region
127-129Views:79Winter barley is the third most important fodder plant in Hungary after winter wheat and maize.
Its accommodation to the changing climatic conditions – mainly to drought in Great Cumania, the driest region of our country is of great importance from the point of view of plant breeding and growing.
The vegetation period of 2002/2003 can be considered to have been very droughty with 247.5 mm precipitation.
According to our results six- rowed barley lines have better drought tolerance than two-rowed lines. The average yields of the six-rowed barley lines were 12-25 per cent higher than the yields of the two rowed lines.
Due to the joint effect of delayed sowing and drought, significant yield depression was detected both in the case of the early and the medium maturity group, and assessable data could be gained only from 35 per cent of the total territory. -
Agronomical and economic evaluation of various cultivation systems on meadow soil
103-106Views:122The requirements and objectives of cultivation are in constant change. There are different cultivation aims if the objective is soil protection, the prevention of its moisture content or on areas with different precipitation supply or production site endowments. Based on the experimental database of the Institute for Land Utilisation, Regional Development and Technology of the University of Debrecen, Centre for Agricultural and Applied Economic Sciences and the KITE Plc., the various cultivation systems in Hajdú-Bihar country were examined with maize as indicator plant. The sample area can be found in the outskirts of Biharnagybajom on meadow soil. On the examined plot, spring strip basic cultivation, loosening and autumn ploughing were applied on 15-15-15 ha, respectively. At the time of taking undisturbed soil samples, soil conductivity measurements were also performed with a Penetronik penetrometer. Undisturbed soil samples were taken from each treatment before sowing (on 5th April 2012). The yield obtained in the strip cultivation treatment increased that of the ploughing and the loosening technology. The economic indexes are the most favourable in the strip cultivation.
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Evaluation of critical factors determining the profitability of sweet maize
97-102Views:137The main goal of this analysis was to determine, whether the production of sweet corn competitive is from the point of view of profitability and identify the main factors determining profitability. The hypothesis of this research was that sweet corn production is profitable and output factors (yields, selling prices) affect most significantly the profitability. The total costs of production with irrigation are 560 000 HUF ha-1. Average yield is 18 t ha-1, while selling prices on average of the last 5 years were about 38 000 HUF t-1. The realisable profit in the sector was 248 828 HUF ha-1. Direct cost-related profitability was 48.6% and profit level 26%. Elasticity analysis pointed out that the changes of selling prices and yields affect profitability. The critical value shows the turning point of profitability, which was at the yield of 13.82 t ha-1. It can be concluded, the hypothesis was true, because sweet corn production was profitable compared to other field crops.
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Effects of the cropyear and the agronomical factors on agronomical elements of different sweet corn (Zea Mays L. convar. saccharata Koern.) genotypes in long-term experiment
105-110Views:116In the crop season of 2010 (rainy year), we studied the effect of three agrotechnical factors (sowing time, fertilization, plant density) and four different genotypes on the agronomical characteristics of sweet corn on chernozem soil in the Hajdúság. The experiments were carried out at the Látókép Experimental Farm of the University of Debrecen. In the experiment, two sowing dates (27 April, 26 May), six fertilization levels (control, N30+PK, N60+PK, N90+PK, N120+PK, N150+PK) and four genotypes (Jumbo, Enterprise, Prelude, Box-R) were used at two plant densities (45 thousand plants ha-1, 65 thousand plants ha-1). The amount of precipitation in the season of 2010 was 184 mm higher, while the average temperature was 0.8 oC higher in the studied months than the average of 30 years. Weather was more favourable for sweet maize at the first sowing date, if we consider the yields, however, if we evaluate the agronomical data and yield elements (number of cobs, cob length and diameter, the number of kernel rows, the number of kernels per row) it can be stated that the size of the fertile cobs was greater at the second sowing date due to the lower number of cobs. The largest number of fertile cobs was harvested in the case of the hybrid Enterprise (72367.9 ha-1) in the higher plant density treatment (65 thousand ha-1) at the fertilization level of N120+PK when the first sowing date was applied. The largest cobs were harvested from the hybrid Box-R (cob weight with husks: 516.7 g, number of kernels in one row: 45.7) at the lower plant density (45 thousand plants ha-1) in the second sowing date treatment. Cob diameter and the number of kernel rows were the highest for the hybrid Prelude.
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Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:91Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
Response of maize seedlings to the latent zinc deficiency under different Fe/Zn rati
125-128Views:103Zinc (Zn) deficiency is a critical nutritional problem for plants and peoples all over the world. Almost half of the world’s cereal crops are deficient in Zn, leading to poor crop yield. In this study, the effect of different Fe/Zn ratio on some physiological parameters of maize seedlings were investigated on the dry matter of shoots and roots and their ratio, SPAD index and the total length of shoots. The relative chlorophyll contents significantly decreased under increasing Fe given to the Zn deficient. The results showed that the different Fe/Zn supplies decreased the total length of shoots - ranging from 9% to 65% - by latent Zn deficiency. Corresponding to several scientific findings, it was observed that the non-optimal Fe/Zn ratio of tissues take part in the evolution of latent Zn deficiency in the case of high Fe concentration.
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Effects of some herbicides on the microbiological characteristics of soil nitrogen cycle under maize plantation
93-100Views:89Nitrogen is a key element for the living organisms and influence not only for the quantity but for the quality of the yield, considerable. Availability of nitrogen from the soil is influenced by several microbiological processes of the Nitrogen-cycle. Among the intensive agricultural production the herbicide application cannot be omitted more information needs therefore about the inhibitor effect of herbicides on the different microorganisms.
An experiment was set up on calcareous chernozem soil under maize culture. Effect of four different herbicides (Acenit, Frontier, Merlin, and Wing) was investigated. The effect of herbicides was measured to four microbiological parameters of the Nitrogencycle (abundance of nitrifying bacteria, nitrate solubilisation, biomass nitrogen and urease enzyme activity). There were singledouble- and five times of recommended doses of herbicides applied for two onsecutive vegetation periods.
From the results of the different doses of herbicides, the following can be stated:
– The Acenit has a stimulating effect on nitrifying bacteria in general. The Frontier and Merlin also influenced the quantity of nitrifyers, however in certain cases decreased in another cases increased the number of bacteria.
– The double doses and five times doses of herbicides was found to be increasing the nitrate content of soil, -especially in 2006.
– The quantity of microbial biomass nitrogen increased in the 60% of treatments and decreased in the 40% of the treatments.
– Except of the result of Wing in 2006 and Merlin in 2005, the effect of simple dose herbicides was the smallest on the urease enzyme activity. According to the results the effect of Merlin was positive; the effect of Wing was negative on the soil enzyme’s activity.
Regarding the application of four different herbicides in three different doses on the microbiological parameters of soil (at two consecutive years-) in 39% of the treatments has resulted a significant inhibitory effect, 28% of the treatments, however have significant stimulating effect on the parameters measured. More than 50% of the inhibitory effect was measured in case of the Wing, at more than 50% of the Frontier the microbiological parameters have not changed. -
Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program
45-51Views:115In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials.
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Economic questions of precision maize production on chernozem soil
293-296Views:132It is one of the main topical objective to establish the conditions of sustainable farming. The sustainable development in crop production also calls for the harmony of satisfying human needs and providing the protection of environmental and natural resources; therefore, the maximum consideratio of production site endowments, the common implementation of production needs and environmental protection aims, the minimum load on the environment and economicalness. Precision farmin encompasses the farming method which is adjusted to the given production site, the changing technology in a given plot, the integrated crop protection, cutting edge technologies, remote sensing, GIS, geostatistics, the change
of the mechanisation of crop production, and the application of information technology novelties in crop production. Modern technology increases efficiency and reduces costs. The efficiency of crop production increases by reducing losses and the farmer has access to a better decision support information technology system. In addition, we consider it necessary to examine the two currently most important economic issues: “is it worth it?” and “how much does it cost?”. During the analysis of agricultural technologies, we used the precision crop production experiment database of KITE Zrt. and the Institute for Land Utilisation, Regional Development and Technology of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen.
During our analytical work, we examined three technological alternatives on two soil types (chernozem and meadow). The first technology is the currently used autumn ploughing cultivation. We extended our analyses to the economic evaluation of satellite navigationassisted ploughing and strip till systems which prefer moisture saving. On chernozem soil, of the satellite-based technological alternatives, the autumn ploughing cultivation provided higher income than strip till. In years with average precipitation supply, we recommend the precision autumn ploughing technological alternative on chernozem soils in the future. On meadow soil, the strip till cultivation technology has more favourable economical results than the autumn ploughing. On soils with high plasticity – considering the high time and energy demand of cultivation and the short amoung of time available for cultivation – we recommend to use strip till technologies. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:178We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Challenges and agroecological approaches in crop production
75-89Views:178Never has the need been greater for an ecosystem approach to agriculture. As our global population exceeds 9 billion in the next 30 years, with a concomitant demand for agricultural products, ever more pressure will be placed on our agricultural systems. Meanwhile, climate change is altering the ecological settings in which agriculture is practiced, demanding adaptation. Knowledge generated by long-term research will help to address one of the grand challenges of our time: how to meet sustainably the growing world demand for agricultural products – in a way that minimizes environmental harm and enhances the delivery of a diverse array of ecosystem services.
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A dual infection of two microscopic fungi on common milkweed (Asclepias syriaca) in Hajdúság region (East-Hungary)
189-195Views:195Common milkweed (Asclepias syriaca L.) is one of the most noxious and invasive weed species in Hungary. A. syriaca invades arable lands, horticultural and forestry plantations, natural and semi-natural habitats too. In cases of field crops it can cause considerable yield losses mostly in maize (2–10%), soybean (12–32%) and sorghum (4–29%), but only with high rate of coverage. It can also increase these problems that the common milkweed can be serve as reservoir and host for viruses, other pathogens and pests.
Because of the importance of common milkweed and in spite of demand to develop effective biological control, until now has not been developed a proper control program against A. syriaca. The aim of our research was to identify the necrotrophic fungal pathogens, which were involved in notable disease occurrence on this weed in different parts of Hajdúság region of Eastern-Hungary in 2016.
To the isolation of fungi from leaves and their identification were based on morphological colony characters on potato dextrose agar (PDA) and Sabouraud dextrose agar (SDA). To the description of conidia features were used PDA for Alternaria and synthetic low-nutrient agar (SNA) for Fusarium species, respectively. The examination of axenic cultures revealed that the fungi isolated from the leaves of common milkweed were Fusarium sporotrichioides and Alternaria alternata.