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Penicillium chrysogenum antifungal protein (PAF) production in transgenic tobacco (Nicotiana tabacum) plant
77-82Views:114Under the „Molecular farming” research program (product vaccines and substances for medical use with gene manipulated plant) in 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture Department of Plant Biotechnology experiments were launched to transform tobacco plant by PAF antifungal protein. Our aim was to learn the transformation technics. We chose the
Nicotiana tabacum and PAF as model systems.
Our work was to express several different paf constructions in plants with nuclear and plastid transformation too. After that we confirmed the presence of paf gene in the level of DNA and RNA. -
Different ideas on the pathogenesis and treatment of swine edema-disease
45-48Views:217Although literature data associate the reason of swine edema-disease with certain serotypes of Escherichia coli bacteria, the authors assume that the primary cause of edema is more different. Susceptible agents and factors, mostly of feed compound are involved. During the digestion of some feed-origin protein opiate-like metabolites, exorphins arise, simultaneously arrest the release of acetylcholine. Consequences of acetylcholine shortage are spasm of sphincters (mostly pylorus), intestine-dilatation, contraction of bladder-sphincter, and urine retention. The endorphins and exorphins intensify the insulin release from the pancreas, hypoglycemia evolves, which is associated with loss of balance. According to the authors in edema-disease piglet dies because of hypoglycemia.
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Development opportunities of biomass-based ethanol production in relation to starch- and cellulosebased bioethanol production
71-75Views:161The biomass is such a row material that is available in large quantities and it can be utilizied by the biotechnology in the future. Nowadays the technology which can process ligno cellulose and break down into fermentable sugars is being researched. One possible field of use of biomass is the liquid fuel production such as ethanol production. Based on the literary life cycle analysis, I compared the starch-based (first generation) to cellulose-based (second generation) bioethanol production in my study considering into account various environmental factors (land use, raw material production, energy balance). After my examination I came to the conclusion that the use of bioethanol, independent of its production technology, is favorable from environmental point of view but the application of second generation bioethanol has greater environmentally benefits.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:236The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Physical and chemical treatment of poultry feather from the slaughter-house
51-56Views:90The 15-20% of the by-products of meat – and poultry industry – that unsuitable for human consumption – contains keratin. The slaughter technology of poultry produces large amount of poultry feather with 50-70% moisture content. This means more million tons annually worldwide (Williams et al., 1991; Hegedűs et al., 1998). The keratin content of feather can be difficulty digested, so physical, chemical and/or biological pretreatment is needed in practice, which has to be set according to the utilization method.
Our applied treatments were based on biogas production, which is a possible utilization method. In the IFA (TULLN) Environmental Biotechnology Institute the feather was homogenized, and – according to the previous examinations – the most effective 1:2 feather-distilled water ratio or 1% NaOH-solution was used, and then treated with microwave (70, 130, 160 °C) during 1 hour time period. DM% and oDM% content was analyzed in the original samples, and the pH, Carbon-, Nitrogen-content in the output, too. Based on the received correlation coefficients (R) and related significance values (Sig.) I concluded, that the C-, N-content and the pH values weren’t influenced by any of the additives. The temperature
affected all three tested factors. The temperature showed a strong coherency with the N-content and the pH value when distilled water was used and weak-medium coherency with the Carboncontent. With NaOH-solution treatment the temperature gave strong coherency with the C- and N-content, as well as medium coherency with the pH. Our objective was to determine the method with effectively the pre-treating of poultry feather for biogas production or composting and to prepare of the treated samples for N and C analyzing. Our next aims will be the elaboration of the technological parameters of heat pre-treatment and microbial digestion of poultry feather for biogas production. -
‘Kindergarten’ keeping-system in farrowing house: effect the socialization of piglets on weight performances, fecal cortisol metabolite level and post-weaning behavior
167-174Views:186This study aimed to examine whether litters’ let-together system before weaning (‘kindergarten-system’) has any stress effect and effect on post-weaning behavior, respectively. One week before weaning piglets were socialized by removing four adjacent farrowing crate walls. After weaning, piglets from the same experimental groups were housed in the same rearing crates. The piglet’s body weight was measured at birth than weekly till the 7th life-week. Fecal cortisol metabolites were measured for evaluation of the adrenocortical activity. Personal observation and evaluation were carried out for behavioral analyses after weaning. There were no significant differences in weight development and cortisol metabolite levels between the control and experimental groups. Behavioral analysis showed that pigs grow up in a socialized system, rapidly evolve ranking in the rearing crates, and the self-maintenance and social behavior were more pronounced. In control groups, the activity involving movement (exploring, walking, and running) was much more decisive. Recent work suggested the beneficial effect of the ‘kindergarten’ system in the farrowing house in animal welfare aspects.
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Micropropagation of Leuce-poplar clones and its role in selection breeding
43-48Views:192Leuce-poplars (mostly white poplar and its natural hybrid grey poplar) are native tree species trough Hungary. They are covering more than 4.0 per cent of the Hungarian forested area (77 000 ha). The white (grey) poplars play a significant role in the forestation under sandy soil site conditions as well as they are of importance from nature conservation point of view as well.Long-term selection breeding work is going on at the National Agricultural Research and Innovation Centre Forest Research Institute (NARIC FRI), involving selection of fast-growing Leuce-poplar clones under dry site conditions.Micropropagation technology is relatively quickly spreading in forestry. In vitro multiplication of trees is applied mainly in fruit growing in Hungary, in case of forestry it is used mainly for selection breeding.This paper presents a short overview on the micropropagation trials with different Leuce-poplar clones and the early evaluation of the seedlings growing of the micropropagated clones/varieties. -
Food problem and its solution
19-23Views:128The Globalization processes substantially changed relations between countries. One of main factors of these processes is forming in ХХ century of Worldwide auction organization, which activity provided with swift growth of volumes in trade of food commodities. Thus, the solution for global food problem was found. At the end of the second half of ХХ century the production volumes of agrarian goods in countries with a different level of economic development grew considerably. However, a food crisis 2007–2008 led to the necessity to define factors, which would allow to grow production, converting an agrarian sector into sustainable growing industry, passing ahead the increase of quantity of population. -
Energy production systems of phototroph microorganisms (classification of photobioreactors)
35-39Views:72In the field of alternative energy sources there is an argue in the comparison of its effects on the benefits and disadvantages to the economics and the environment. New studies are born which are in contradiction with each other. The demand for bioenergy feedstock is growing rapidly however there are the environmental problems caused by the extending energy crop plantations. There is such a significant need for land to grow traditional energy crops on (rape, soy, palm-oil, sugarcane, etc.) that the food purpose agricultural capacity could be in danger. Probably the extensively
growing energy crops play a role in the very high prices of food. In some countries like China for example laws prohibit the use of food based crops such as corn for energie production. In the case of corn based ethanol production the cost only for the feedstock itself is over 60% of the whole preparation costs which significantly effects the entire economy of the energy productions process.
The microalgaes however have a huge biotechnological potential and their production is notably cheaper then the traditionaly grown food crops growing expenses. They play a significant role as feedstocks in todays industrial production in such fields as comestible production, cosmetics, pharmaceutics and biotechnology especially in biofule production. In the field of economy the major aspects here are also the technological designs
and the construction. For the future industrial production the closed type systems seem to have more advantages compared to the open, pond-type systems. For high value material production the more innovative and more easily developed closed photobioreactors are the profitable regardless the vast techological designs used in the construction. -
A simple method for preparing elemental selenium nano- coating inside a silicone surface
35-43Views:275Selenium nanoparticles (SeNPs) with a bright red colour have aroused worldwide attention due to their unique properties in selenium supplementation because of their low toxicity and favourable bioavailability. A simple method was developed for making a red selenium nanolayer on the inner surface of Polyvinyl chloride (PVC) and silicone tube. The selenium nanoparticles were produced by the reaction of sodium selenite and ascorbic acid. Red amorphous selenium nanoparticles have been successfully synthesized by the reaction of 500 mg dm-3 Se (sodium selenite) solution with 10 g dm-3 ascorbic acid solution at room temperature, and morphology was confirmed by X-ray diffraction analysis (XRD). The coating density was compared on PVC and silicone surfaces by using Scanning Electron Microscopy (SEM) and Energy-dispersive X-ray (EDS) analysis. The nanolayer with about 16 µm thickness on the silicone surface significantly evenly distributed compared to the PVC surface. The selenium coated silicone tube could be a good source of selenium for a continuous, low-level selenium supplementation of farm animals via drinking water.
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Effect of noninvasive castration method on weight gain, behavior and meat quality of ram lambs
21-29Views:178In the course of producing heavyweight lambs (above 35 kg), males need to be separated from females at the end of the fattening period. If not, the rams must be castrated because they reach sexual maturity, and their activity bothers the ewes or unwanted pregnancy may occur. The present study surveys if the Hungarian sheep keepers know or use the non-surgical elastrator method for castration and assess the effect of castration (surgical and non-surgical) on daily weight gain, behavior, moving activity, and meat quality of rams, respectively. We found many advantages regarding the use of the elastrator method. Based on the survey results, 100% of farmers who used elastrator had a positive experience and favorable opinion about this method. There is no need to separate the rams, which allows for less area requirement and more economical technological conditions. The traditional castration (with a knife) process is longer (4–6 minutes), and caused longer-lasting stress while the elastrator application is bloodless, took only 20–30 seconds, and were stress-free. The number of steps of ring-gelded individuals was much lower than that of the non-castrated rams. The difference in steps number could also be seen in ewes separated into different ram groups. The weight gains of individuals castrated by the ring were better than the surgically castrated ones and also individuals with testicles. The palatability of the meat from the non-castrated group was less favorable, and the chewiness of the ringed group was the best. Finally, our results highlighted the benefits of the noninvasive elastrator method in animal welfare aspects.
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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:325Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Application of mycorrhizae and rhizobacteria inoculations in the cultivation of processing tomato under water shortage
111-118Views:113The effect of mycorrhizal fungi and plant growth promoting rhizobacteria on some physiological properties, yield and soluble solid content (Brix) of ‘Uno Rosso’ F1 processing tomato was studied under water scarcity. Inoculation was performed with mycorrhizal fungi (M) and rhizobacteria preparation (PH) at sowing (M1, PH1) and sowing + planting (M2, PH2). The treated and untreated plants were grown with regular irrigation (RI = ET100%), with deficit irrigation (DI = ET50%) and without irrigation (I0). In drought, the canopy temperature of plants inoculated with arbuscular mycorrhizal fungi (M1, M2) decreased significantly, however, the decrease was small in those treated with the bacterium (PH1, PH2), while the SPAD value of the leaves of plants treated only with Phylazonit increased significantly. On two occasions, inoculations (M2, PH2) significantly increased the total yield and marketable yield, however, under water deficiency, a higher rate of green yield was detected than untreated plants. In dry year using deficit irrigation, the one-time inoculation (M1, PH1) provided a more favorable Brix value, while the double treatments reduced the Brix. In moderate water scarcity, the use of mycorrhizal inoculation (M2) is preferable, while under weak water stress, the use of rhizobacteria inoculation (PH2) is more favorable.
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Alternatives of the multiple use of Virginia mallow (Sida hermaphrodita L. Rusby) as a shrub-replacing mallow in the temperate climate zone
51-56Views:172In the seventies of the previous century, Dr. Zoltán Kováts set two directions in the research of mallows. One of the directions was the biotechnology of the mallow species and the other direction is using the mallow species as biomass material. In order to do this he brought mallow mother spawns of ornamental and biomass sorts from botanical gardens abroad and tested many of them, including the a Sida hermaphrodita kind. Fourty years later, for the second time this plant, known as the „energy mallow” got back to Hungary again, right into the sight of hungarian biomass business with the help of László Balogh and his associates using help from Poland. This genus got into the center of our research, because of it’s valuable attributes. The latest experiments are about using it as an energy plant, without examining genetic details. The plant grows up to more
than 3 meters, has high growing rate and produces big amount of green mass. We don’t have any hungarian data about whether the plant continues the sufficient growing rate or not, after cutting it back.
We have to explore the potentials in the Sida’s sublimation. The plant is mostly suitable for ornamental and energy utilization, so further sublimation should be aiming for these aspects. During my research period, we are willing to get to know these potentials and the best possible use of them. -
Fungi detected in trunk of stone fruits in the Czech Republic
121-127Views:240This study was focused on detection of the spectrum of fungi in the wood of stone fruits using molecular genetic methods. Samples were obtained from apricots, plums and sweet cherry trees from region of Moravia, one sample was obtained from Myjava (Slovakia). Segments of symptomatic wood were obtained from dying stone fruit trees with very significant symptoms. This study describes detection of the fungi in the wood of 11 trees in general in 5 localities. The cultivation of the fungi from symptomatic wood and sequencing of ITS was carried out. Eleven fungal genera were determined in the stone fruits wood, particularly Irpex lacteus, Fomes fomentarius, Neofabraea corticola, Calosphaeria pulchella, Cytospora leucostoma, Phellinus tuberculosus, Stereum hirsutum, Collophora sp., Pithomyces chartarum, Aureobasidium pullulans,Fusarium sp. The results of this study demonstrate that the reason of declining of stone fruit trees in Moravia is caused probably by trunk pathogens.
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Review on the fatty acid profile and free fatty acid of common carp (Cyprinus carpio)
99-105Views:161Carp or ponty in Hungarian, is considered commercial freshwater fish, which is an adaptable species in both wild and cultured conditions. Carp has high nutritional value content, favorable taste, it is rich in protein, and low in saturated fat. The nutritional content in fish is composed of many chemical constituents and influenced by many factors. One of the components that its content may be different due to internal and external factors is fatty acids, which may vary depending on endogenous and exogenous factors. The endogenous or internal factors include the genetic, size, sexual maturity, and life cycle phase. While microclimate, water quality, quality of food or diet habit, and the amount of available food or starvation are considered as exogenous or environmental factors. Freshwater fish has the ability to convert essential fatty acid into long chain polyunsaturated fatty acid like AA, EPA, and DHA. Most results showed that palmitic acid and oleic acid were the dominant SFA and MUFA in carp both for wild and farmed carp in all seasons. The PUFA for wild carp was mainly dominated by DHA, while on farmed carp by LA. It confirmed that high LA content in farmed carp was related to the diet habit. The amount of lipid and FA were changed in line with the season. Even the statistical analysis showed no significant difference, but some studies showed a contrasting result. Moreover, most obtained results acknowledged that FA tends to decrease during the spawning period. The amount and composition of FA were affected by the total lipid content. The lipid must be broken down into simpler compounds such as FA or FFA for the metabolism of fish. The result of metabolism then transported into the utilising tissue and used as energy.