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Cytochrome b diversity of Hungarian Botrytis cinerea strains
18-21Views:284In the mitochondrion of eukaryotes, cytochrome b is a component of respiratory chain complex III. Cytochrome b is encoded by the
cytochrome b (CYTB) gene located in the mitochondrial genome. The fungicidal activity of QoIs relies on their ability to inhibit mitochondrial respiration by binding at the so-called Qo site (the outer quinol-oxidation site) of the complex III. Since their introduction, QoIs (like azoxystrobin) have become essential components of plant disease control programs because of their wide-ranging efficacy against many agriculturally important fungal diseases like grey mould on various crops. QoI resistance primarily arises from a target-site-based mechanism involving mutations in the mitochondrial CYTB. As the management of grey mould is often dependent on chemicals, the rational design of control programs requires the information about the diversity of genes connected with resistance in field populations of the pathogen.
Monospore B. cinerea field isolates has been collected during 2008-2009 from different hosts in Hungary. PCR fragment length analysis
indicated the high frequency presence of type large intron in the isolates while in a few strains G143A substitution could also be detected.
These results indicated the heterogeneity of CYTB in the Hungarian B. cinerea populations, which possibly involve the heteroplasmy of this
mitochondrial gene, moreover indicates the existence op azoxystrobin resistant populations in Hungary.
This work was supported by NKFP-A2-2006/0017 grant. Erzsébet Fekete is a grantee of the János Bolyai Scholarship (BO/00519/09/8). -
Characteristics of roe deer bot fly infection in the southern part of the Great Hungarian Plain
57-60Views:306I am investigating the occurance of one of the diseases caused by parasites in plain roe deer populations, namely a botfly larvae, Cephenemyiastimulator, (Clark, 1815) ranged among the Oestridae family. Nasal botfly larvae are common roe deer parasites and are generally spread nationwide. The subject of the present survey is the processing of the data gained about roe deer bucks of the year 2009 and their infestation indices. I investigated botfly larvae in 90 bucks’ nasal and pharyngeal cavity. We have found botfly larva in 18.9% of the examined bucks (prevalence), this means 17 infected specimen. I collected 218 pieces of larvae from the samples, with a 12.8 pieces average intensity. In my investigations prevalence was lower and mean intensity was higher than in previous Hungarian researches. This was manifested both in my overall data, and in our examinations by the different age groups.
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Marketing analysis of the Villány wine region
119-123Views:311Hungarian wine is not in such a bad position as it is considered. We have outstanding specialists and the country’s natural conditions are also proper. In Hungary, there are wine production and wine consumption has a major traditions. The role of marketing in our wine industry is growing more and more, and this trend is expected to continue in the future. The development of tourism in wine-growing regions and advertising would considerably promote the popularity of Hungarian wine.
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Toxic elemental content of Hungarian blaufränkisch wines
87-90Views:579Toxic elemental contents are one of the food safety risks in wines. Therefore International Organization of Vine and Wine (O.I.V.) defined the limit of some elements in it. Thirty Hungarian blaufränkisch wines were analysed by ICP-MS in order to determine the concentration of toxic elements. All wines are passed by the O.I.V limits to toxic element content (Cu, Zn, Cd, Pb) according to analysis. Copper is the only outlier in some samples, but they also did not overstep the limit. In the case of zinc the maximum is approximately fourth, in case of cadmium and lead the highest concentration is less than tenth and sixth of the O.I.V. limit, respectively.
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Global and national economic importance of pig meat production
13-20Views:622The pork production has changed significant in recent decades. Pig production fell significantly due to the need to adapt to new animal welfare rules in the sector. Furthermore, change in the meat consumption habit had also a negative effect on pig meat production. Hungarian pig sector and meat industry are in a difficult situation. Due to the high feed prices, the average production cost of pig farming has often exceeded the producer prices in recent years, therefore an increase of pig population could not be realised yet. Pig market is characterised by cyclical fluctuations of supply and prices, furthermore the decreasing producer price occurred parallel with increasing feed prices.
Technology is also a critical fact in the Hungarian pig sector because there is a huge gap in the level of technology compared to that of competitive member states. With the development of production methods the efficiency of pork meat production can be improved in an environmentally friendly way. -
E-business in Hungarian Tourism
81-86Views:188Thanks to communication tools, globalization is spreading wordwide. For this reason, it is crucial to protect and strengthen our feature. On the one hand, we are repetitive joining the uniting world of economics, on the other hand, it is our responsibility to preserve our national characteristics with conscious politics.
Responding tardy to the touristic demands of the information society without risking the loss of our competitiveness market is highly dangerous at the current situation of the touristic, as the Hungarian Economic Development Program sets down. Unless we develop a strategy that realized radical changes in the next 4-5 years, Hungary can find itself in a disadvantageous situation regarding the touristic market of the 21st century. -
Influence of Hydrological Parameters on the Biodiversity of the BerettyóRiver I.
215-229Views:395The DIRECTIVE 2000/60/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL orders the measurement of the state of natural and artifical waters. We therefore applied environmental qualification methods, which allow inductions of the searched area in environmental management, conservation and water quality protection points of view. These methods are accepted in interdisciplinary sciences, and are in use in Hungarian and international practice. We applied the range data measured along the Berettyó River, and ratified by the remote sensing, to count hydrological-hydraulical attributes of the stream, by the framework of the Hec-Ras programme. We processed the physical, chemical and biochemical water qualifications, determined the MMCP-index (the point-system of the Hungarian macrozoobenthos taxons), and estimated the Spencer index-numbers, which were based on complex environmental qualification and bioindication. Appointed, that the different results of researches are not inconsistent. This results together the spatial analysis reveal the natural and anthroponetic specialities of the river and the landscape, which determine the environmental flavours and biodiversity. The bioindicators represent better environmental status, than the physical, chemical and biochemical parameters. The natural conservationist importance of drainage canal network of Bihari-Plain is appreciated by these facts.
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Development Level Analysis of the Settlements and Micro Regions of the Hungarian-Ukrainian Borderland
73-78Views:208This paper aims to make a settlement and micro region-level analysis in case of one of Hungary’s most backwater regions, the Hungarian-Ukrainian borderland. After a brief methodological and theoretical review, it creates a complex development indicator with the help of certain ratios belonging to appropriate groups. It analyzes the exceptionally heterogeneous settlement-level values with applied statistics methods and tries to determine the role of population, population density, the distance from the nearest border-crossing point and the travel time from the centre of the micro region and the county. As its main findings the followings can be mentioned: there are significant development level differences in case of the „periphery of the periphery” micro regions which depend on the number of population, population density and the travel time from the centre of the micro regions.
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Search for polymorphism in exon 5 of cattle pituitary adenylate cyclase activating polypeptide gene
17-20Views:630Pituitary adenylate cyclase-activating polypeptide is a neuropeptide expressed primarily in the hypothalamus and in other tissues, which divergent and extensive physiological functions are proved. Large number of SNPs in PACAP are published involved SNP that is associated with phenotypic trait of cattle as well. Hungarian Grey, Hungarian Simmental, Holstein, Charolais and Angus cattle breeds were involved in this study to search for polymorphism in exon 5 1–391 bp and G/A transition. Our results of PCR RFLP and PCR SSCP did not prove the occurrence neither G/A transition, nor any other SNP in cattle breeds involved.
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The effect of collecting area on the element content of Hungarian acacia honeys
129-138Views:555Six macroelements and twelve microelements were identified in thirty-six Hungarian acacia honeys collected from ten counties by inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). One-Way ANOVA (LSD and Dunnett T3 test) and linear discriminant analysis (LDA) were used to determine the statistically verified differences among the honey samples with different geographical origin.
Significant differences were established among the samples from different counties in Na, P, S, Fe, Ni, Cu and Sr concentrations. Based on the macroelement content of honeys, the separation of samples with different geographical origin was not successful because the percent of correctly categorised cases was only 64.9%. However, examining the As, B, Ba, Cu, Fe Mn, Ni and Sr concentration, the separation of different groups was convincing since the percent of correctly classified cases was 97.2%. Thus, the examination of microelement concentration may be able to determine the geographical origin of acacia honeys.
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Evaluation of Hungarian Sporthorse mare performance tests
83-87Views:410Results of the Hungarian Sporthorse mare performance tests were evaluated. Data from the period of 1993-2009 were used, covering
scores of 618 3-year-old and 310 4-year-old mares, 109 of them were tested at both ages. Seventeen traits were scored on the tests, which
covered ten conformational, three free jumping performance and four movement analyses traits, respectively. Breeding value estimation was
based on BLUP animal model. Test year, age and owner were included in the model as fixed effects. Variance components were estimated
with VCE-6 software package. Heritabilities ranged from 0.32 (frame) to 0.50 (saddle region) for conformation traits, from 0.39 (jumping
style) to 0.49 (jumping ability and jumping skill) for free jumping traits and from 0.20 (walk) to 0.48 (canter) for movement analysis traits.
Breeding value indexes were constructed for each trait group. Conformation index was computed based on the weighted scores of the
breeding values of conformational traits. The conformational score scales were used as weightings. Free jumping and movement indexes
contain the proper breeding values with equal weights. A total index was also constructed using conformation index, two times the free
jumping index and two times the movement index. Each breeding values and breeding value indexes were presented with the mean 100 and
standard deviation of 20 for the easier understanding. -
Comparison of major population parameters of Brown hare (Lepus europaeus, Pallas 1758) in two hunting fields of the Great Hungarian Plain
69-74Views:402Hungarian stock of game is not only part of our national treasure but also one of our domestic products and economic income. Not indifferent therefore the number and the state of health of our wildlife. Population decline of the Brown hare (Lepus europaeus, Pallas 1758) (one of our most important small game in Hungary) takes a long time. Demographic parameters of Brown hare was examined, particularly the factors affecting the decline of the species in Hungary. We took samples from typical habitats where the Brown hare could be found in relatively high density in our country. The article reports data of reproductive characteristics, diseases, parasites of Brown hare and other factors such as climatic and anthropogenic which could influence of the population dynamics. We mention sample collection and processing methods eg: population size estimates, examination of reproductive organs, the sex ratio and the age structure as well as the individual condition based on data of domestic and foreign authors and our partial results.
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Use of molecular marker methods in the classification of bamboo taxa: A review
51-59Views:473Bamboo plants are currently attractive to researchers because of their versatile uses. Understanding the bamboos’ genetic level is needed to develop new varieties. Taxonomic identification is the basis for plant development. Bamboos were identified as their taxonomical morphological characters which are dependent on environmental factors. Molecular Marker techniques can be used to perform accurate genotype identification, which can be used for genetic diversity analyses. The RFLP, RAPD, AFLP, SSR, ISSR, iPBS, SCARS, SCoT, SRAP marker systems have been shown to be able to efficiently determine the genetic diversity of bamboo species based on genotyping. This paper summarizes research that aims to analyze the genetic diversity of bamboo species on a molecular basis.
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Morphological and leaf anatomical studies on Cleistogenes Serotina (L.) Keng (Poaceae)
5-13Views:189The grass species Cleistogenes serotina (L.) Keng, generally found in the mountainous region in Hungary, has been observed in the lowlands of the Great Hungarian Plain in the last few decades. Since its first recent mention in sand habitat as well it, has been found in several locations in the interfluve between the Danube and the Tisza. In certain, sandy grasslands, the invasion of this species is considerable (e.g. near Fülöpháza the area invaded by Cleistogenes increased almost fourfold within six years). The success of invasion on new habitats is related to changes in the
characteristics of plants comparatively studied on mountain and sand populations.
We studied blades of leaves on different levels (1st, 5th and 10th) and from different biotops. Cuttings were made, with a thickness of 10-12 μm, at the middle of the blades. The cross sections were observed using a light microscope. Digitised images (DigitPlan) and survey data were arranged into a database.
The leaf structure is characterised by the kranz anatomy, and a double layered bundle sheath, which is typical of the aspartate type of C4 grasses. There were significant differences only in cross section area and the number of bundles for various ages of the leaves. There were more bundles in the older leaves than in the younger ones. We did not find any difference in the ratio of tissues among leaves of different ages and sources. According to our study, the ratio of leaf tissues characteristic to species was neither significantly influenced by the biotop, nor by the age of the leaves. However, there is a difference in tissues size. Summarising the results, the species can be considered „only” as ecotypes on the
different studied areas.
Our new result is that kranz anatomy can also be found in the leaf-sheath.
This research was supported by the Hungarian Scientific Fund (OTKA), grant No. T016511 and T026346. -
Analysis methods of quality and traceability for honey
25-32Views:647Honey is our essential food since ancient times. Thanks to the excellent dietetic and medical features, it has an important function in our nourishment. In Hungary about 25.000 tons of honey are produced each year. Most of the produce, about 80% is exported to different countries of the world. Hungarian types of honey have excellent quality. Through exporting, the prestige and demand of Hungarian honey can be increased. In recent years the adulteration of honey caused many damages to producers and traders. The adulteration of honey is not easy to prove. Thereinafter, we represent several methods, such as oligosaccharide analysis or 13C/12C isotopic ratio analysis, which can identify the artificial substances of honey (for example: corn syrup, high fructose corn syrup) or can help to determine the ratio by which original honey can be identified.
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Creating potential agricultural clusters in the Northern Great Plain Region
63-67Views:205The crisis of the hungarian agricultre is continuous since the ’80s. The compensation is made a dual farm system, which has created barriers to efficient agricultural production. It is confirmed by the economic indexes as well. But the most important natural resources of our country is still the land. Over the past 20 years, the government was not able to work out strategic aims and adequate system for the hungarian agriculture. In the past three decades the clusters has become the most common tool in economic development worldwide. Clusters always base on some local knowledge, and a country can be competitive on the industry in which the resources are concentrated. So the question is, is it possible to „clusterize” the
agriculture and through this develop the rural economic. -
The Carpathian lingonberry, raspberry and blackberry fruit extracts feature variable antimicrobial efficiency
27-32Views:563Wild berry is an excellent source of phytonutrients and/or bioactive compounds associated with significant therapeutic properties, so that they have been utilized in folk medicine and traditional nutrition throughout centuries. Multiple health-promoting effects, such as anti-inflammatory, anti-diabetic, anti-heart and coronary disease properties were attributed to such wild berries. It has also been proved that berries could feature antimicrobial effects that could be of a great importance for the prevention of food-feed poisoning and fighting back antibiotic resistance.
In this study, we investigated the antimicrobial properties of lingonberry (Vaccinium vitis-idaea), raspberry (Rubus idaeus) and blackberry (Rubus fruticosus) crude and ethanolic extracts prepared from fruits obtained from the spontaneous flora of Eastern Carpathian Mountains situated in Transylvania. The antimicrobial effect of crude and alcoholic extracts were assessed on four Gram-negative, five Gram-positive bacteria and one yeast species using the agar diffusion method. The studied bacteria can cause food or feed spoilage and foodborne diseases. Our results indicate the significant inhibitory effect of lingonberry extracts in the case of Gram-negative bacteria like Proteus vulgaris and Salmonella Hartford, while among Gram-positive bacteria the strongest inhibitory effect was observed for Bacillus species like B. cereus, B. subtilis, B. mojavensis and Micrococcus luteus. The raspberry and blackberry extracts featured milder inhibitory effects in the case of the studied bacteria species. Furthermore, we have studied the crude or ethanolic extract combinations associated antimicrobial effects synergistic/additive or antagonistic properties. Interestingly, the triple and double ethanolic extract mixes had stronger antimicrobial properties, whereas the crude extract mixes showed relatively reduced effects, if any. Our results indicate that the antimicrobial activity of studied fruit extracts obtained from wild berries can vary upon the applied extraction method and their combination formulae, so that all these considerations must be taken into account when such fruit extracts are considered for foodstuff development.
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A review on fish in vitro digestion studies
55-63Views:150(1) Background: In vitro experiments in fish have been developed to search for dietary substitutes of fish meal in aquafeeds by measuring the digestibility of various feedstuffs by simply simulating the conditions of the stomach or portions of the digestive tract. In vitro digestion studies involving mainly commercial fish species have been conducted to determine the digestibility of conventional and alternative ingredients in fish diets using diverse digestion models. However, there remains a significant knowledge gap, particularly regarding the enzyme functionality, digestion mechanisms in many fish species, and the factors influencing enzymatic activity. This review article is focused on the importance of the use of different enzyme sources in the in vitro digestion model to predict protein hydrolysis.;(2) Methods: For this review, a comprehensive analysis of articles was conducted to gain insights into diverse enzyme interactions and feed evaluation assessments, from the nutritional science and biotechnology sector.; (3) Results: A total of 36 peer-review papers, which include original, and review articles were selected.; (4) Conclusions: In vitro digestion studies offer a valuable tool for evaluating the digestibility of conventional and alternative feed ingredients, helping to identify sustainable dietary substitutes for fish meal while reducing dependence on live animal trials.
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Analysis of changes in resources and outputs of companies and partnerships in Hungarian agriculture
65-68Views:237The aim of the study was to examine the achievement of Hungarian agricultural companies and partnerships, particularly agricultural co-operatives based on the aggregated database of National Tax and Customs Administration (NAV)1. From the methodological aspect, descriptive statistical methods and time series analysis were used. One of the most important conclusions is that the socio-economic weight of the agricultural co-operatives was strongly decreased in the period after the EU accession. The other important statement is that apart from the general examination of economic actors on aggregated level the comparative analysis of the single organizations forms would be needed. In the case of co-operatives, separated examination of the former type producer co-operatives and the so-called new type ones (e.g. marketing co-operatives etc.) would be necessary.
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Daily behaviour of Hungarian Grey Cattle under range grazing conditions
45-48Views:368Cattle behaviour on rangeland depends on external factors, such as grass allowance and quality, temperature, net solar radiation, distance from water-source, wind speed and direction, air pressure changes and the applied breeding technology. Our research is based on previous empirical observations and modern methods to analyze the behaviour of the Hungarian Grey Cattle. Today it is crucial to use cost-effective solutions in modern beef cattle farming therefore we introduce a cost-efficient method to study and follow cattle herds. We are studying relation between traveled daily distance and air pressure. The various weather fronts influence behavioural characteristics and traveled daily distance. According to our hypothesis, the pressure-change and the wind direction has significant effect on cattle activity on pasture. As the different air masses alter the barometric conditions and unbalance the neuroendocrine system, indirectly cause relaxed or agitated behaviour
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Antimicrobial effect of dried sage on the microbiological state of fresh Hungarian sausage
189-192Views:303The purpose of this study was to evaluate the microbial effect of dried sage (Salvia officinalis L.) on the traditional Hungarian sausage. We added 0.5, 1, 1.5, and 2 w/w% of sage to the sausages and tested them on the 0th, 7th and 14th day. The added dried sage had no effect on the tested microorganisms, but the sage extract inhibited Salmonella, Enterococcus faecium and Staphylococcus aureus.
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Nitrogen Content of Hungarian Soils and Nitrogen Fertilization
51-61Views:237For crop production and agricultural production, the most important natural resource is the soil that can optionally renew. Paralelly with this, soil plays a major role in the geological and biological cycle of elements. As a result of the big (geological) and small (biological) element cycles, the elements and combines neccessary for organisms can accumulate in the soil creating suitable living conditions for plants and other organisms. Soil is a heterogenous system both horizontally and vertically, and soil constituents show great variety in all the three dimensions, in addition, most of the parameters can also change between two examination dates. When talking about the factors influencing plant production, one should take into account this variation and heterogenity in time and space. When making fertilization recommendations, these factors should all be considered. In any consultation system, most of the mistakes and errors made are due to the unsatisfying soil testing and the negligence of soil heterogenity. In the practice of fertilization the biggest mistake is the improper soil sampling, then comes the methodical mistake of soil testing, which is followed by the inaccuracy of instrumental analysis and the subjectivity of result evaluation, but the latter two are negligible compared to the others. Under normal, i.e. production conditions, the quantity and distribution of nutrients in the soil are greatly dependent upon the applied technology, the amount and form of the applied natural and artificial fertilizers and the quality of fertilization.
Fertilization recommendations are needed because in the layer which is accessible for plant roots only a part of the nutrient content is available for plants in a specific production cycle. An illustration of this is that though the upper 1 m layer of an average chernozem soil contains more than 5000 kg N, 12000 kg K2O and 1500 kg P2O5 (form of expression mostly used in Hungary), the application of fertilizer doses which are just fractions of these quantities is essential. This is due to the fact that the available amount of the total nutrient content depends from the quality of soil, the environmental factors (the physical and chemical qualities of the soil) and the specific nutrient’s qualities (solubility, adsorption). Knowledge of these processes and the examination of the factors influencing the actual nutrient content are vital for working out a fertilization practice, which does not put more strain on the environment than neccessary.All of the above mentioned should be considered when applying inputs in the fields. In a well-functioning practice that considers the economic and environmental conditions (unfortunately the present production and economic conditions do not enable an appropriate level and degree), three nutrients are supplemented generally (and were supplemented in the last decades): nitrogen, phosphorus, potassium.
Studying the nutrient balance of the Hungarian field production’s last hundred years, we can draw some interesting conclusions.
The nutrient balance became positive for nitrogen and potassium in the second half of the 1960’s, while for phosphorus it was positive from the first half of the 1960’s and this period lasted until the end of the 1980’s.
Neither before the 1960’s, nor since the 1990’s has the amount of nutrients supplemented in a specific year reached the amount of the nutrient uptake of the same year. -
Characterization of Hungarian touristical turnover 2006-2009 – focus on health tourism
47-50Views:233Tourism is one of the biggest and mostly increasing branch all over the world. Since it is getting more and more significant in Hungary as well in this article I analyze the turnover of Hungarian tourism on the basis of guest nights spent on commercial lodgings. I examine its changes in time and its territorial spreading. Finally I explore the health touristic volume, because it is one of the most important touristic product of Hungary and the North Plain Region and the infrastructure based upon preserves unique opportunities both for health and wellness tourism.
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Labour productivity in the Hungarian agriculture
119-128Views:299This paper presents the situation of the agricultural labour employment and evaluates the agricultural labour productivity in Hungary. Since Hungary’s EU accession, the share of agricultural employment in the total employed population has been stabilized at around 5% in the country. Due to low wages, low profit and low prestige, agricultural jobs are not attractive. The gross and net labour productivity gap between Hungary and the EU have been significant since the EU accession. The agricultural labour productivity of the Hungarian regions also shows a different picture. The labour productivity influences the cost, profitability and competitiveness of products. The advantage of the cheap labour force in Hungary’s agriculture significantly decreased due to low labour productivity. There are several opportunities to increase the agricultural labour productivity such as the increase of labour force qualification, moving toward producing higher value added agri-food products, rejuvenation of population in agriculture and the improvement of the conditions of financing agriculture.
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Possibilities of conceptualization and operacioanlizaton of the safety culture and safety climate in the international safety management researches
117-126Views:163In this paper the author summarized the definitions and operationalisations of the safety culture and safety climate concepts. He analyzed the Hungarian and international safety
management scientific literatures. He also analyzed the scientific definitions and summarised the common and different elements. The author emphasizes that the many different scientific safety culture researches had been created good possibilities to find the common surfaces. Based on the international safety culture operationalisation practices the author identified a composition of safety culture dimensions which could be a possible basis for the Hungarian safety culture researches. These dimensions are:
???? values (individual and organizational) from this result scale of values,
???? attitudes (individual and organizational),
???? motivations,
???? perceptions,
???? social status and estimation of the occupational safety and health,
???? position and estimation of the occupational safety and health communication,
???? position and estimation of the occupational safety and health tranings,
???? measuring of confidence, optimism, fatalism and anomic characteristics,
???? good practices and behavior,
???? influence and estimation of the work,
???? risk perception and safety level perception,
???? contentment.with occupational safety and health.