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Inner pelvic measurements in dairy breeds
31-35Views:453Dystocia causes great financial losses: due to dystocia milk production is decreased and the probability of calf loss is increased. There are many factors that may cause dystocia. One of the factors –often investigated in beef cattle- is pelvic measurements. There have not been inner pelvic measurement comparisons done on dairy breeds in Hungary.
After comparing the imported, primiparous cows, Jerseys turned out to have the smallest absolute inner pelvic measures. According to their age and weight, Brown Swiss cows had the largest pelvic dimensions. Ayrshire, Norwegian and Swedish Red, the three dairy breeds which share similar genetic background did not differ in most measures. Holsteins were closest to the apparent ideal 1:1 horizontal and vertical diameter ratio; however, this breed suffers the most from dystocia. Jerseys, despite having the smallest pelvic area are famous of their calving ease, perhaps not by coincidence. Although this dairy breed is the lightest, when the pelvic area was compared in ratio of body weight Jerseys were not smaller than the 130 and 114 kg heavier Norwegian and Swedish Red cows. -
The role of agriculture in regional development
25-30Views:780Agriculture is the leading national economic sector of Hajdú-Bihar county. The proportion of employees in this sector and the share of pro ducts produced in this region is above the country average. The contribution of agriculture to the GDP is 6.3%, as opposed to the average value of the country (3.3%). Based on their primary activity, 8% of the enterprises in the county are classified as agricultural and forestry companies, while this proportion is only 5.3% in the whole country.
Nearly three quarters (71.1%) of the sowing area is occupied by maize and other cereals, mainly wheat. This proportion is slightly more than the country average (69.2%). The proportional area of oil crops - including sunflower – and roughage is nearly the same as the country average. However, there is a significant difference in vegetable production. Due to the higher intensity of vegetable production, the area occupied by vegetables is three times the country average. 19.4% of the crop products, 23.5% of animal products and living animals, 25.8% of the income generated by production factors and 29.8% of net enterprise income of Hungary originates from the North Great Plain region.
The facilitation of creating local markets, e.g. local public food supply, satisfying the needs of food trade with local goods, the necessary awareness raising actions, secondary and higher education of experts, as well as the more specific and targeted utilisation of development resources are agricultural development tasks.
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Pest species of Macrolepidoptera in the Game Reserve of Velyka Dobron’ (Transcarpathia, Ukraine)
58-64Views:574The Game Reserve of Nagydobrony extends on a marginal area of a former peatland and is covered with extended hardwood gallery forests and oak-hornbeam forests and is surrounded by a mosaic-like agricultural landscape. Due to its richness of nature-like and semi-natural habitats it supports a diverse insect assemblage. By light and bait trapping 383 species of macro-moths were recorded from which larvae of 85 species are feeding either on forest trees and scrubs or on cultivated plants thus these can be considered as potential pest species. Thirteen species (mostly Geometridae and Erebidae: Lymantriinae) have a special significance for forestry due to defoliating activity in gradation periods. Considering the habitat connections, the composition of moth assemblage is dominated by generalist species with broad spectrum of ecological tolerance but the species connected with humid forested habitats are also richly represented. The bulk of species consists of widely distributed Euro-Siberian species, but also some Holo-Mediterranean species with more southern character and Mediterranean-Subtropical migrant species were registered. The bait trapping provided significant results on the phenology of the dominant species. The faunistically significant and/or protected species were observed in a low number of individuals only, thus the applied trapping methods did not damage the faunal composition.
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Comparison of different pheromone traps for monitoring of click beetle Agriotes ustulatus (Coleoptera Elateridae)
155-159Views:350The effectiveness of two trap types (YATLORf and VARb3 with CSALOMON® sex pheromone bait) for monitoring click beetle Agriotes ustu latus was compared near Karcag (East Hungary) in 2016. Additionally effectiveness of YATLORf traps placed on ground and placed on a 25 cm high mound was also compared. Contrary to our expectations traps caught very few individuals thus our study could provide only preliminary results. Between traps and methods there were not significant differences but YATLORf traps placed on mounds caught more individuals than others. The swarming imagos of A. ustulatus were also detected two weeks earlier in 2016 than as usual. To prove the effect of the way of usage on the effectiveness of YATLORf traps new studies should be made.
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Investigation of Chromium(III)-Picolinate Adsorption on Some Soil Types
190-193Views:302In the experiment adsorption characteristics of different soil types (humic sand, meadow soil, leached chernozem and meadow solonec) was examined on the basis of adsorption isotherms for Cr(III)-picolinate. The Langmuir equation was used to describe the isotherms by which the amounts of metal ions actually and maximally adsorbed by the soils were determined concerning the given complex. A comparison was made among the organically bound Cr(III)-picolinate, an inorganic Cr(III) compound and a Cr(VI) form examined in a previous study. Based on the adsorption isotherms, adsorption capacity of the Cr(III)-picolinate was found 20 times smaller on sandy soil and 50 times smaller on the chernozem comparing to that of the inorganic Cr(III)-chloride, thus, the bio-availability of the chromium for the plants is 20 and 50 times higher in case of the given soil types. For the well-known toxic Cr(VI)-form, the adsorption was 2 times higher in case of sandy soil and 5 times higher for chernozem than in case of the organic Cr(III)-complex compound.
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The study of headspace sampling for the measurement of Volatile Organic Compounds byGC-MS
35-38Views:469Headspace sampling was studied for GC-MS measurements. A quick, direct method was developed for the measurement of petrol, gasoline and volatile organic solvent contamination of soil. According to our results this method is suitable for quick qualitative analysis of VOC contamination of soil, and helps to make decisions about further measurements. We concluded that an accurate quantitative measurement is not possible.
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Horn colour varieties of the Hungarian Grey cattle
83-87Views:429The Hungarian Grey Cattle breed is a ‘success-story’ of the national genetic conservation work. Traits of the breed have been subjects of several research projects, although many relationships remained unclear. Our present research results were meant to call attention to a less emphasized trait of the breed: the different horn colour varieties. Research work was carried out in the Hungarian Grey stock of the Hortobágy Non-profit Company for Nature Conservation and Gene Preservation. Ratio of the three main horn colour varieties (white, ‘cardy’, green) were determined in the observed female, male and steer stocks. Our results showed no significant differences (P<0,05) in the distribution of the horn colour varieties of the female and bull stocks, and of the female and steer stocks. We found that on the basis of the ratio of white colour, four sub-categories can be distinguished within the ‘cardy’ colour variety. Results of the statistical analysis (P<0,05) confirmed that the colour of the horn and the ratio of the black part on the horn tip are two different traits.
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Opportunities of utilization of real-time ultrasound technique in beef cattle breeding
45-52Views:401The goal of the authors was to evaluate the repeatability and accuracy of real-time ultrasound technique under Hungarian conditions. Ribeye area was measured at first time on live animals, before the day of slaughtering with Falco 100 ultrasound equipment in Charolais fattening bulls. Ultrasound pictures were traced at two times by the same operator. After slaughtering, ribeye area (REA) was measured on the carcass by planimeter at the same anatomical point like ultrasound measurements. Statistical analysis was carried out to examine the relationship between carcass and ultrasound REA. After it, repeatabilty was established by comparing the ultrasound pictures twice.
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”FOR CROP PROTECTION” Establishment medallion in honour of Antal Gulyás (1884-1980)
5-10Views:295The Public Utility for Development of Crop Protection Teaching (NOFKA) and The Hajdú-Bihar County Regional Association of Hungarian Chamber of Crop Protection Specialists and Plant Doctors (Chamber) established a joined Award Committee in September of 2011, which intend to serve as moral appreciation to prominent persons with excellent achievements by award Antal Gulyás medallion for crop protection“ which are available for outstanding teachers, researchers, and practical plant protectionists.
The members of Committee: dr. István Szarukán, president, dr. György Kövics, secretary, dr. István Dávid member (Public Utility), dr. László Kiss, president, dr. Gábor Tarcali, secretary (Chamber). Handing over of medallion, charter and gold badge will be happened generally once a year in a special ceremony. The award was established in commemoration of Antal Gulyás, who was the first eminent professor of plant protection in Debrecen and reached outstanding achievements in the field of teaching of agronomists for more than 30 years, and research on plant pathology. He became one of the first Honoris Causa Doctors of Debrecen Agricultural University in 1978. The Committee made its decision on the first meeting that the first medallion will be award to dr. Oszkár Tóth retired reader of plant pathology for his excellence in teaching plant protection in 2011. His laudation is available in a separate article of this issue. -
Comparative analysis of Carpathian Braunvieh’s morphological traits
15-19Views:646The Carpathian Braunvieh cattle established by the cross-breeding of the Schweizer Braunvieh and the local breeds of the Carpathian basin approximately 150 years ago. The evolved three usage breed was durable and resistant, however in comparison with the high-productivity breeds was less competitive. The dramatic lay-off, and the endangered status of the breed requires a conservation programme. University of Debrecen took the investigation of the Hungarian population and it’s detailed genetic studies. Within this research project among others we carry out body measurements and rates of the body traits. Depending on these results, we may decide on the subsequent gene-reserving objects. Based on body measurements and live weight we established that the breed at Mikóháza favours to the one lived in Hungary in the 1960’s, and to another, named Ukrainian Carpathian Brown. Compared to the earlier data (Horváth, 1966) smaller body traits can be seen. Our livestock is proportionately smaller than the other breeds in height at withers, as well as in live weight. Based on the comparative analysis, we established, that – thanks to the aware sorting – the imported individuals represent the ancient, primitive Carpathian variant.
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Optimal age of breeding gilts and its impact on lifetime performance
15-20Views:962Age at first breeding and lifetime reproductive performance was analyzed on 17,558 F1 Landrace x Yorkshire gilts from 9 piglet producing herds of Midwest, United States entered in herds between 1st Jan 2014 and 31st July 2016. At the time of data collection Dec 2018, 15% of the sows were still active in the herds hence excluded from the analysis. Individual gilt data included date of birth, age in days at first mating, piglet total born by parity, lifetime piglet total born and reason for culling. Quality data checks were done before analysis to eliminate all outlier values together with sows that had no entry information for any listed category. The total database of the sows was classified into 6 classes according to age at the first mating in days 170-190(n=754), 191-211(n=4683), 212-232(n=7123), 233-253(n=3385), 254-274(n=1002) and 275-369 (n=611). Piglet total born obtained from each sow during the lifetime production was significantly (P < 0.05) greater for gilts bred between 233-253 days of age at first mating. Gilts that were bred at <233 days appeared to have a higher risk of removal by farrowing productivity as compared to the other groups. However, the results show that the risk of being culled due to health problems and conformation issues increases as the age at first mating is delayed. Overall reproductive failure appears to be the most economical culling reason across all age groups. There is a need to evaluate the best management decisions for gilt initiation in a herd to maximize her lifetime performance. The results indicate that gilts mated for the first time at the right age, 233–253 days, are more productive, both in lifetime total born and have a minimal risk of culling due to farrowing productivity.
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Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:246We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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Following-up organic pollutants in the course of producing foods
244-248Views:620We analysed the raw materials in various food products, including additives, carrier solvents and end product for several selected organic pollutants. We analysed these food products for the type of chlorinated hydrocarbon pesticides, polychlorinated biphenyls and other currently used pesticides.
We analysed three product categories: bakery products, canned mushroom and meat products. The analysis of the bakery products and of the canned mushroom did not conclude in positive findings, which means that these products contained the agents given during the production of the product and the environmental pollutants in undetectable low concentration.
However, the results for meat and fish products indicate that polychlorinated compounds are persistent and are of a lipophyl character, as some of these components have been detected in these food products, the concentration of the PCBs were detected in the range of 9.8-24 μg/kg. -
Study of traditional folk foods from the point of view of nutrition in Hajdú-Bihar County
47-51Views:496Healthy nutrition is one of the major topics of our days. The occurrence of the so called “civilization diseases” is increasing continuously as a result of the changes in our nutritional habits and life-style. It was thought that folk nutrition at the beginning of the 19. Century had several beneficial effects on health. For this reason it was decided to study the food and nutritional habits of the population of Hajdu-Bihar County around the 1930s on the
basis of literary sources to see how well they do match with modern requirements of healthy nutrition. -
Studies of plant density increase – on maize hybrids of various genotypes on chernozem soil
87-92Views:541The yield and crop safety of maize are influenced by numerous ecological, biological and agrotechnical factors. It is of special importance to study one of the agrotechnical elements, the plant density of maize hybrids, which is influenced by the growing area conditions and the selected hybrid.
We have investigated the effects of three different plant numbers (50 thousand plants ha-1, 70 thousand plants ha-1 and 90 thousand plants ha-1) on the yield of 12 maize hybrids of different genotypes in Hajdúság, on calcareous chernozem soil, in the Látókép Research Farm of the University of Debrecen, Centre for Agricultural Sciences, in 2013. The experiment was set in four replications, besides commonly applied agrotechnical actions. In the experiment, 1 hybrid of very early (Sarolta), 9 of early (P 9578, DKC 4014, DKC 4025, P 9175, NK Lucius, Reseda, P 37N01, DKC 4490, P 9494) and 2 of medium (Kenéz, SY Afinity) maturation were used.
With the increase of the plant number, the number of individuals per unit area increases. According to our experimental results, we have concluded that with the increase of the plant number, the yield increased in the average of the hybrids. In the average of the hybrids, in the case of 50 thousand plants ha-1, the yield was 13 130 kg ha-1, in the case of 70 thousand plants ha-1, it was 13 824 kg ha-1, while in the case of 90 thousand plants ha-1, the yield became 13 877 kg ha-1.
In addition to plant density increase, it is necessary to determine the optimal plant number that is the most favourable for the certain hybrid under the given conditions. To fulfil this aim, we have determined the optimal plant number corresponding to the maximum yield of the given hybrid, within the given plant number range. The optimal and applied plant numbers differ, since the optimal one could only be applied under ideal conditions. Since the agrotechnical actions cannot always be carried out in appropriate quality and one has to adapt to the weather conditions, thus we have determined a plant number range in the case of each hybrid. The hybrids were classified into categories of producible in narrow and broad plant number range.
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Measurement of the degradation of abandoned turf
145-149Views:365With the decreasing number of grazing livestock in Hungary, the role of the turf cultivation is also significantly decreasing. The proportion of the under- and non-utilized turf is increasing. In the research conduced at the University of Debrecen, IAREF Research Institute Karcag, we studied four types of turf utilization in three replicates on a salt field with timothy grass. We determined the flora composition of the experimental area, measured the soil moisture and the carbon-dioxide content of the soil.
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Occurrence of the tulip tree aphid (Illinoia liriodendri Monell, 1879 (Hemiptera: Aphididae) in Hungary
93-96Views:277The genus Illinoia is found primarily in North America. Illinoia liriodendri (Monell, 1879), the tulip tree aphid, has been observed and caught for the first time in Hungary. Nymphs, apterous and alate viviparous females in colonies of I. liriodendri were found on a tulip tree, Liriodendron tulipifera L. (Magnoliaceae), in Debrecen, Hungary. Tulip tree aphid feeds on the underside of tulip tree leaves. The consequences of this are honeydew and associated black sooty mould causing a mess – in countries where the tulip tree is native or abundant - for walking people and parked cars. A short report is presented here on the discovery in Hungary and characteristics of this aphid.
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Inner pelvic measurements in dairy breeds
51-56Views:519Dystocia causes great financial losses: due to dystocia milk production is decreased and the probability of calf loss is increased. There are many factors that may cause dystocia. One of the factors –often investigated in beef cattle- is pelvic measurements. There have not been inner pelvic measurement comparisons in dairy breeds in Hungary.
After comparing the imported, primiparous cows, Jersey turned out to have the smallest absolute inner pelvic measures. According to their age and weight, Brown Swiss cows had the largest pelvic dimensions. Ayrshires, Norwegian and Swedish Red, the three dairy breeds which share similar genetic background did not differ in most measures. Holsteins were closest to the seemingly ideal 1:1 horizontal and vertical diameter ratio; however this breed suffers the most from dystocia. Jerseys, despite having the smallest pelvic area are famous of their calving ease, perhaps not by coincidence. Though this dairy breed is the lightest, when pelvic area was compared in ratio of body weight Jerseys were not smaller than the 130 and 114 kg heavier Norwegian and Swedish Red cows. -
In vitro analysis of the effect of ragweed extract against Monilinia laxa
117-120Views:214Nowadays in Hungary nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia). According to the public opinion the ragweed is a weed. From agricultural and public health point of view it is exceptionally dangerous plant. As it contains a number of useful active ingredients, based on this the ragweed is consider a medicinal plant. Our goal was to present that the ragweed contains antifungal active substances as well. In the experiments we used the pre-flowering plants with roots and we extracted the biological active components of dried plant. We tested the biological activity of the extracts against Monilinia laxa in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Monilinia laxa.
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The impacts of spring basal and side dressing on maize yield
83-86Views:362The yield potential of maize is very high. According to Tollenaar (1983), maize yield potential is as high as 25 t ha-1 (absolute dry yield) which is the highest among all cereals. In order to fully utilise this high yield potential, proper nutrient replenishment is of chief importance among all agrotechnical factors.
The aim of research was to examine the effect of nitrogen fertiliser applied as basal and side dressing on maize yield.
The measurements were performed at the Látókép experiment site (47° 33’ N, 21° 26’ E, 111 m asl) of the Centre for Agricultural Sciences of the University of Debrecen on mid-heavy calcareous chernozem soil with deep humus layer in an established experiment in 2011, 2012 and 2013. The trial design was split-split-plot with two replications.
Based on the experiment results, it can be established that the nutrient uptake of maize is greatly dependent on the amount of water store in the soil. From the aspect of the development of the maize plant and water supply, the most determinant factor was the distribution of precipitation over the growing season and not the amount precipitation. This is shown by the fact there was only 276 mm precipitation – which was favourably distributed – in 2012 to increase the availability of nutrients and the main average was the highest in this year (14.394 t ha-1).
Spring basal dressing helped maize development in all three years even on chernozem soil which is well supplied with nutrients. Although the effect of side dressing did not result in any yield increase, it could still contribute to mitigating the stress effects caused by environmental factors. Altogether, nutrient supply adapted to the various development stages of maize can favourably affect the success of maize production.
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Recent data about the fish consumption of great Cormorant in the area of Hortobágy Fish Farm Co
75-80Views:502The cormorant (Phalacrocorax carbo) is a bird species that nests sporadically but in colonies, besides larger fishponds and rivers in Hungary. The number of its individuals has been increasing during the last two decades. The species eats solely fish, therefore it can cause serious depredation of the fish stocks in fishponds of intensive system and after the freezing of the ponds, in larger rivers, which are not yet frozen. The aim of our research was to reveal the damages the birds can cause on the studied areas and the extent of the losses the Hortobágy Fish Farm Co. has to realize.
Our studies were carried out between April 2012 and November 2014. During cleaning, the investigation of the craw contents of the birds and their biometric studies were conducted. The results revealed the diverse food base of the species. In the samples, we have determined 379 fish individuals; in 368 cases, the exact fish species were determined, too. Significant differences were found between the consumed fish species rations of the units (P=0.05% beside). We have also found significant difference in the fish consumptions of adult males and females.
Under the ever harder fish production conditions, the presence and thus the permanent predation of the birds affect the fishermen. In favour of maintaining ecological balance as well as to hold the income of the fish farmers, elaboration of adequate protective and preventive methods will be needed in near future.
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Investigating the potential for dual-purpose use in winter oats
35-42Views:507Dual-purpose usage is not a common technology for oats in Hungary, which is not surprising, as the area sown to our main crop is also very low. Yet the crop has a lot of untapped potential, not to mention its excellent nutritional parameters. In our experiment we investigated how much biomass can be expected after mowing winter oats at different phenological stages and whether we can expect a valuable grain yield after this process. Mowing was carried out at one-week intervals on a total of 4 occasions with 2 cutting heights. Plant height and NDVI values of the vegetation in the plots were measured during the experiment. After the harvest of the fully ripened grains, the yield and the thousand kernel weight (TKW) were determined. Our results show that with a lower cutting height (6 cm), a higher biomass yield can be achieved. The cutting height had no statistically verifiable effect on either NDVI or plant height. Droughty weather conditions during subsequent mowing significantly reduced the regeneration capacity of the plants, which was strongly reflected in the measured parameters. Furthermore, a higher grain yield was measured at a cutting height of 9 cm, which decreased gradually with the mowing time. Neither the cutting height nor the mowing time had any effect on the TKW.
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Comparison of the physiological responses of an oil-pumpkin cultivar and hybrid under different nitrogen supply conditions
65-69Views:535Cucurbita pepo var. styriaca is known as a medicinal crop among other cucurbits. The benefits of cucurbits fruits are very important in terms of human health, purification of blood, removal of constipation, digestion and supplying energy. The mutant styrian oil-pumpkin (Cucurbita pepo L. convar. Pepo var. styriaca Greb.) exposes a complete lack of lignification of the seed testa. For this reason, this kind of naked pumpkin seed is more consumable as snack than other seeds. The vegetative growth of plants is crucial for fruit production. Nitrogen is the most imperative element for the proper vegetative growth and development of plants which significantly increases and enhances yield and its quality by playing a vital role in the biochemical and physiological functions of plants.
Field studies were conducted to study the effect of nitrogen nutrition on certain physiological parameters and their differences between the styrian oil pumpkin hybrid and cultivar (Gleisdorfi Classic cultivar and GL Rustical hybrid). The small block experiment was carried out based on a factorial experiment with a completely randomised block design and four replications in the Research Institute of Nyíregyháza during the summer period of 2017 and 2018. Three different concentrations of nitrogen fertiliser was applied at sowing. Photosynthetic pigments (chlorophyll-a, -b and carotenoids) were analysed as one of the basic factor of efficient photosynthesis. The yields of two genotypes were also measured in all treatments as seed-weight. The contents of photosynthetic pigments were higher in the oil-pumpkin hybrid, mainly the carotenoids in 2018. The vegetation period of 2018 was drier than in 2017, the hybrid oil-pupmkin was more efficient in stress tolerance than the cultivar with higher carotenoids pool. The obtained yield was more pronounced in the oil-pumpkin hybrid than the cultivar in all examined years. The hybrid GL Rustical was more sensitive to nitrogen treatment than the cultivar Gleisdorfi Classic.
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The Effect of Atmosperical Aridity on the Changes of Quantitative Parameters of Horticultural and Arable Crops
40-45Views:397The occurence of atmospherical drought causes serious water-supply problems in the most cases of our domestic agricultural plant species. This paper was studied, how can we quantificate the atmospherical drought, with the help of a low input (relative humidityof the air, temperature) index. If this index (LSZI) characterized the atmospherical drought well, it will suitable to estimate the yield amount of agricultural plants.
The index elaborated by the authors was tested on county average crop yield of 14 agricultural plant species. Moreover we compared the atmospherical drought index (LSZI) to other aridity parameters, how suitable for estimate the yield amount.
Result of experiments show that, the atmospherical drought index (LSZI) can be used well by several agricultural plant species in especially coern and sugar-beet to estimate yield amount. Excellent results were found by comparison to other aridity indexes, this means it is worth using in the aridity researches in the future. -
Phylogenetic studies of soybean pathogen Phoma species by Bayesian analysis
53-61Views:513We carried out phylogenetic study analyzing sequences of genetic markers in the taxonomy of Phoma and Phoma-like fungi. Different species of Phoma and Phoma-like fungi occurring on soybean (Phoma pinodella, Phoma sojicola, Phyllosticta sojicola, Phoma exigua var. exigua) are difficult to identy because of their high morphological and symptomatic similarities.
Twenty-two isolates of nine different Phoma species were obtained from reference culture collections. Seven of them were isolated from soybean, the others were collected from different hosts.
The Phoma isolates were firstly characterised by morphologically, and then we employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1), ITS region, as well as β-tubulin partial sequences as potential genetic markers to infer
phylogenetic relationships among different Phoma species..Finally, their ITS and tef1 sequences were sequenced and analysed by Bayesian approaches.
According to phylogenetic trees inferred by Bayesian analysis of tef1, ITS and β-tubulin sequences, different Phoma species can be separated proving that these phylogenetic markers are well suited for phylogenetic studies of Phoma species. However, the phylogenetic tree does not support the traditional Phoma sections based on morphological characterization.
Bayesian analyses of the three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The molecular data provide evidence for reclassification of formerly mentioned soybean pathogens.