No. 39 (2010): Special crop protection issue
Remembrance to László Huzián (1923-1996)5-11Views:68
László Huzián was a determining personality of the higher education of Hungarian crop protection more precisely that of the agricultural entomology. Training of the scientific and extension specialists of Hungarian agricultural entomology from 1960 till 1983 would have been unimaginable without him. He was the builder of the agricultural entomology school established by Gusztáv Adolf Manninger, the developer of the training’s essential and organisational standard, who sacrificed his life’s work for developing the crop protection. He found, characterized many pests (E.g. Scrobipalpa ocellatella, Lixus scabricollis, Tanymecus palliatus, Mesagroicus obscurus) new for the Hungarian fauna and worked out a control technology against them. It is important to mention his activity in creating the forecasting of the big field crop animal pests mainly that of the sugar beet pests. One top of his educational work was the wonderful manual „Agricultural Entomology” written with Sándor Bognár in 1979. László Szalay-Marzsó said of this book that it was impossible to write a better one. He has been right. The magnificent crop protection library and the unique richness and accuracy of the animal demonstration material (slides, pictures, preparates, showcases, etc.) established at the Gödöllő University of Agriculture can be thanked to his extraordinary systematizing mentality and tireless efforts.
Sour cherry anthracnose and possibilities of the control with special regard to resident Glomerella population in sour cherry plantations of East Hungary12-17Views:122
Anthracnose is considered one of the most destructive diseases for sour cherry production due to the rapid development of the disease on fruits. Glomerella cingulata (Stoneman) Spauld. & H. Schrenk (anam.: Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz.) has been the fungal pathogen responsible for anthracnose in last decades. Yield losses greater than 90% may occur under epidemic conditions. C. acutatum (J.H. Simmonds, 1968) strains were isolated of sourcherry plantations in East Hungary and this pathogen, new for Hungarian microbiont became recently dominant. Contrarily to the former species it is certainly transmitted with ants during fruit ripening. About third of strains proved to be cutinase producers that enable them to actively penetrate via cuticule, and these strains infect directly berries of blackberry, grape and tomato as well as plum and apple. Most of cutinase negative strains could also infect these fruits after mechanic injury. All strains of both species produce amylase, cellulase, lecithinase, lipase, polyfenoloxydase and protease in vitro, although the activity of these enzymes highly varied in the medium. The only C. acutatum strains produced noticeable amount of chitinase. Strains, tolerant to recently applied fungicides to control the anthracnose, could be isolated of sour cherry plantations that might be the cause of ineffectiveness of control measures in 2010. The mycofungicide containing mixture of three Trichoderma species in oil carrier could efficiently depress the development of anthracnose in ripening sour cherry.
Cytochrome b diversity of Hungarian Botrytis cinerea strains18-21Views:85
In the mitochondrion of eukaryotes, cytochrome b is a component of respiratory chain complex III. Cytochrome b is encoded by the
cytochrome b (CYTB) gene located in the mitochondrial genome. The fungicidal activity of QoIs relies on their ability to inhibit mitochondrial respiration by binding at the so-called Qo site (the outer quinol-oxidation site) of the complex III. Since their introduction, QoIs (like azoxystrobin) have become essential components of plant disease control programs because of their wide-ranging efficacy against many agriculturally important fungal diseases like grey mould on various crops. QoI resistance primarily arises from a target-site-based mechanism involving mutations in the mitochondrial CYTB. As the management of grey mould is often dependent on chemicals, the rational design of control programs requires the information about the diversity of genes connected with resistance in field populations of the pathogen.
Monospore B. cinerea field isolates has been collected during 2008-2009 from different hosts in Hungary. PCR fragment length analysis
indicated the high frequency presence of type large intron in the isolates while in a few strains G143A substitution could also be detected.
These results indicated the heterogeneity of CYTB in the Hungarian B. cinerea populations, which possibly involve the heteroplasmy of this
mitochondrial gene, moreover indicates the existence op azoxystrobin resistant populations in Hungary.
This work was supported by NKFP-A2-2006/0017 grant. Erzsébet Fekete is a grantee of the János Bolyai Scholarship (BO/00519/09/8).
Yearly change of Wheat dwarf virus infection rate during 1996-2010 in winter barley22-28Views:92
Yearly change of the infection of Wheat dwarf virus was studied in winter barley during 1996-2010. Surveys were carried out at Kompolt (Rudolf Fleischmann Research Institute, Róbert Károly College), in winter barley breeding lines showing leaf yellowing and stunting symptoms. In 1996, 250 winter barley samples were tested. During the period of 1997–2005, 100 samples were collected in each year. In 2006, 490 winter barley samples were tested. In 2007 and 2008 the number of samples collected was 500 from winter barley. In 2009 year 100, and in 2010 year 100 winter barley samples were collected for virus testing. Virus diagnosis was carried out using DASELISA for the detection of Wheat dwarf virus (WDV), Barley yellow dwarf viruses (BYDV-MAV, BYDV-PAV, BYDV-RMV, BYDV-SGV), and Cereal yellow dwarf virus (CYDV-RPV). During the ten of the last fifteen years, the occurrence of Wheat dwarf virus in infected samples exceeded those of other viruses causing leaf yellowing and dwarfing symptoms. There were years (1997, 2002, 2004, 2007, 2009 and 2010) when only the Wheat dwarf virus played the main role in development of yirus symtoms. A contrasting tendency can be observed between the degrees of infection of WDV and BYDV. With a rise of infection in the WDV, the proportion of BYDV decreased and vice-versa.
Inland and foreign Erwinia amylovora isolates by carbohydrate utilization29-33Views:70
Fire blight, a plant disease caused by the bacterium Erwinia amylovora, produces serious losses in apple and pear orchards all over the world. Since the appearance of fire blight in Hungary (Hevesi, 1996) Erwinia amylovora isolates were collected in different years, from different hosts and areas in order to establish gene bank for future epidemiological studies. We had isolates from foreign countries as well. The aim of our research was to compare all of the Hungarian and foreign isolates by carbohydrate utilization.
In our experiments effect of carbohydrates on E. amylovora multiplication was determined using API 50 CH strip (bioMérieux, France). By the API 50 CH strip method we checked a number of unstudied carbohydrates. The results of the tests shows colour changes. Based on utilization of 49 carbohydrates of API 50 CH kit by E. amylovora isolates, two groups of carbohydrates can be defined: “Utilized” - and “Not utilized” carbohydrates. All isolates utilized 20 different carbohydrates after 164 hour incubation. Conversely, isolates also could be divided into four groups (1, 2, 3, 4) by arbutin and raffinose utilization. In group 1.-isolates utilize arbutin; 2.- utilize raffinose; 3.- utilize both arbutin and raffinose; 4. -utilize neither arbutin nor raffinose. Presumably carbohydrate content of nectar could play an important role on invasion of the (E. amylovora) bacterium via flower.
It could be concluded that the carbohydrate utilization – completed with genetic analysis – can be used for characterization of Erwinia amylovora isolates.
Dieback of apricot plantations caused by 'Ca. Phytoplasma prunorum' in Borsod-Abaúj-Zemplén county (Northern-Hungary)34-41Views:96
Plant diseases caused by phytoplasmas have increasing importance in all over the world for fruit growers. Lately, phytoplasma diseases occur on many fruit varieties and responsible for serious losses both in quality and quantity of fruit production. In the long-run these diseases cause destruction of fruit trees. The apricot phytoplasma disease (Ca. Phytoplasma prunorum) was first reported in Europe in 1924 from France. In 1992 the disease has also been identified in Hungary. On the base of growers' signals serious damages of "Candidatus Phytoplasma prunorum" Seemüller and Schneider, 2004 (formerly: European stone fruit yellows phytoplasma) could be observed in different stone fruit plantations in the famous apricot-growing area nearby Gönc town, Northern-Hungary. Field examinations have been begun in 2009 in several stone fruit plantations in Borsod-Abaúj-Zemplén County mainly in Gönc region which is one of the most important apricot growing regions in Hungary, named “Gönc Apricot Growing Area”. Our goals were to diagnose the occurrence of Ca. Phytoplasma prunorum on stone fruits (especially on apricot) in the North-Hungarian growing areas by visual diagnostics and confirm data by laboratory PCR-based examinations. All the 28 collected samples were tested in laboratory trials and at 13 samples from apricot, peach, sour cherry and wild plum were confirmed the presence of phytoplasma (ESFY). On the base of observations it seems evident that the notable losses caused by "Ca. Phytoplasma prunorum" is a new plant health problem to manage for fruit growers, especially apricot producers in Hungary.
Germination and sugar content alteration in maize grain caused by Fusarium contamination42-44Views:56
Healthy and Fusarium affected ears were collected in Fészerlak, Somogy County at the end of vegetation cycle of maize (25-30 August). Each pattern contains 25-25 ears. We compared the samples on the basis of visual image of Fusarium affection. The ears were shelled and two 0,5 kg samples were formed: healthy and Fusarium contaminated. After surface sterilisation the uniform sized seeds were soaked in sterile distilled water for 24 hours and there were germinated for 7 days. The α-amylase activity was measured with Phadebas- α-amylase test. Seeds were extracted one by one three times under reflux using 10 cm3 boiling water for 15 minutes. During our investigation germinating activity was detected to measure glucose, fructose, sucrose content and α-amylase activity. In the first seven days of germination the highest values were detected in control seeds followed by the affected seeds. Our results clearly show that stress conditions applied altered not only the saccharide content but decreased their germinating activity as well in the case of maize grain.
Laboratory diagnoses of the isolates of chestnut blight disease fungus Cryphonectria parasitica (MURR. BARR)45-52Views:79
Chryphonectria parasitica, the casual agent of chestnut blight, is one of the most important fungal pathogens of chestnut (Castanea spp.) in Europe and Hungary. In this study, we analyzed the ITS region of five Cryphonectria parasitica strains isolated from different location of Hungary. The differences among the Cryphonectria parasitica isolates were not insignificant because only two sites were considered as informative for the parsimony analysis. As the differences among geographically different isolates were insignificant, we mean that the evolutionary distance by ITS sequences within Hungarian Cryphonectria parasitica isolates is too small to get well based consequences for the phylogenetic relationships.
Distribution and relative abundance of the economically important click beetles (Elateridae: Agriotes sp.) in Hungary53-60Views:85
Click beetle (Elateridae: Agriotes sp.) species of 24 sites in different regions of Hungary were studied in 2010. A. brevis, A. sputator, A. obscurus, A. lineatus, A. rufipalpis and A. ustulatus were sampled by pheromone traps in maize fields. During the study more than 80000 beetles were caught. The three most common species were A. ustulatus, A. sputator and A. rufipalpis. The distribution of the studied species was uneven. In south Hungary A. ustulatus, A, rufipalpis and A. sputator were the three most abundant species. In the Transdanubia A. sputator was the most abundant. A. ustulatus reached higher abundance in only three sites (3/12). A. obscurus occurred only in west Hungary (Transdanubia). In eastern Hungary the abundance of studied species was higher. In this reason we have to monitor the populations of these pests and if it is necessary we have to take actions against them. In Transdanubia the abundance were generally lower but in many cases reached the threshold of significant damage. Beyond that 13 additional species were sampled so the total number of sampled species was 19.
Monitoring raspberry cane midge (Resseliella theobaldi) on the basis of temperature data61-64Views:80
The ’midge blight’, in which the raspberry cane midge (Resseliella theobaldi) has an important role, is the greatest problem in the raspberry protection. The basis of the chemical protection against raspberry cane midge is the prediction of adult midge emergence. Before the application of sex pheromone traps developed for the investigation of flight pattern, the usage of the accumulated temperature was attempted for the prediction of egg laying, but these temperatures are different in each country. The aim of this paper is to give information on the time of raspberry cane midge emergence and flight pattern by using sex pheromone traps and accumulated temperature calculation.
Phytoseiid mites on conifers65-69Views:57
The abundance and species diversity of phytoseiids were studied in the evergreen collection of the Corvinus University of Budapest at Soroksár from March to August 2010. 329 phytoseiid mite specimens were collected from 15 cultivars of coniferous trees belonging to the following 4 genera: Juniperus, Abies, Picea and Pinus. 6 phytoseiid mite species have been identified: Amblyseius andersoni (Koch, 1957), Amblyseius tenuis Westerboer 1963, Anthoseius bakeri (Garman, 1948), Anthoseius involutus Livshitz et Kuznetzov 1972, Typhlodromus baccetti Lombardinii 1960 and Typhlodromus cotoneastri Wainstein 1963.
The dominant phytoseiid species were Amblyseius andersoni and Anthoseius involutus.
Amblyseius tenuis and Typhlodromus baccettii are new to the Hungarian fauna. Typhlodromus baccettii was found on Juniperus scopulorum 'Silver Star' and 'Moonglow' while Amblyseius tenuis was collected from Picea glauca 'Alberta Globe'.
The occurrence and phenology of moth pests in different granary of Borsod-Abaúj-Zemplén County70-75Views:77
The aims of my studies were the followings: primarily to find correlation between the conditions of granary and the occurrence of moth pests. Secondly I studied the effect of disinfection on individual numbers in the population of moths. My studies were started in May 2009 in six different places of Borsod-Abaúj-Zemplén County. Indianmeal moth (Plodia interpunctella) and Mediterranean Flour Moth (Ephestia kuehniella) traps with pheromone were installed in four repetitions as well as Angoumois Grain Moth (Sitotroga cerealella) traps in also four repetitions. Control traps without attractant were also placed at every place.
From my researches, it became clear that the disinfection alone is not enough to protect cereals from moths. As in the case of crop protection, we need to apply integrated pest management.
We have to make differences between preventive protections from moth pests and the elimination of them by chemicals.
Up to my opinion, the regular checking and cleaning of the granary are important as well as the prognosis of the possible occurrence of moths. The prognosis is considered important because the studied moths do not feed at the adult stage or only at a low level. However, the caterpillars coming from the eggs placed by females can cause a significant damage in the stored cereals.
The studied sex pheromone traps are proved to be useful for the reduction of number of moths since the traps caught lots of them. These traps are relatively cheap because only the temporarily changes of pheromones increase the cost.
Occurrence and damage of the alfalfa root longhorn beetle (Plagionotus floralis Pallas, 1773) in old alfalfa stands in Hungary82-88Views:86
The alfalfa root longhorn beetle is a pest of small importance in old alfalfa stands. It is rarely cited in the international, national literature, or even mentioned in specialized manuels. Plagionotus floralis is common in Hungary but its population density is low. It is a polyphagous species but it has importance - regarding the references – only in alfalfa. This paper analyses data gained in old alfalfa fields at two different regions of the country (Máriabesnyő, Debrecen). Damage of P. floralis was 56% in the roots of an 8-year-old stand in Máriabesnyő. Lenghts of tunnels were between 3 and 22 cm. In spite of the tunnels the alfalfa plants were viable. There was no root longhorn beetle in the 15-year-old Debrecen stand, however it has been found former in the region. The reason for the important density of P. floralis in Máriabesnyő could be the considerable diversity and covering of flowering weeds around the field. Adults of P. floralis feed on flower pollen and nectar. As to the Debrecen area,weeds were cut regularly, so the edge vegetation was poor. Present conditions (no pest control on alfalfa fields, overuse of old and failing alfalfa stands, many untrained growers) favour the reproduction and possible damage of alfalfa root longhorn beetle.
What has Harmonia axyridis to do with Dante's Divine Comedy82-88Views:73
The multicoloured Asian ladybird beetle (Harmonia axyridis (Pallas, 1773)), a former successful biological control agent in the USA and Western Europe became an invasive alien species threatening the diversity of native aphidophagous insects through competition and praying, a horticultural pest consuming various fruits and adversely affecting the wine quality and a human nuisance when occurring at high densities in buildings. Due to this dubious fame, attention has been paid to it also at national level which made inevitable to find for it a fitting name in different languages. In some cases and countries a version of the English name has been chosen however, regarding the meaning of this translated term in Hungarian, the try cannot be called fortunate. The objective of this contribution is to stress the function of common animal names in the Hungarian Sprachraum and to find a right and proper alternative with the help of etymology, mythology, classical literature, art (painting) and the experiences of Hungarian fruit and grape growers.
Sensitivity study on Virginia fanpetals (Sida hermaphrodita (L.) RUSBY) cv PETEMI to different herbicide agents89-92Views:66
The cultivation of Virginia fanpetals (Sida hermaphrodita (L.) Rusby) is a relatively new phenomenon in Europe. On the basis of the biology of the plant and the practical work implemented, it has been stated that the traditional field practice does not provide the appropriate conditions of biomass production for energetic purpose. The development of the proper weed control is inevitable for the healthy stand, as in the early phenophases the plant is growing slowly and it is exposed to weeds.
Our objective was to test some herbicide agents as no previous relevant data had been published.
Observation of some parameters on adult millet (Panicum miliaceum L.) individuals by pre-post herbicides controlling mostly grass type annual weeds93-96Views:55
The experiment was conducted in warmer mean daily temperature than the many years average, and almost in the same precipitation conditition as the many years average. The herbicides employed were sprayed in the 3-4 leaf stages developing phase of the millet (Panicum miliaceum L.) population by the dose of officially proposed. On the basic of the results, the herbicides didn’t effect deleteriously, in most instances, on the cultur plants in sort and long periodus at all. Tendenciously the values of parameters observed were sligtly better after the Stomp 330EC than the Dual Gold 960EC. ( The values of the parameters are significantly better at the treated variants than the control ones, are in realation with the wheather and development stile of the millet, beside the different weeding status of the plots.)
Comparative analysis of maize weed control system and the competitive effect of sorghum97-104Views:79
In our investigation we used different weed control technologies in the different phenology states of the maize. The experiment have been
carried out in Hódmezővásárhely, in the Experiment garden of the Pilot farm of University of Szeged Faculty of Agriculture, on meadow
chernozem soil, on 24 m2 plots, in 3 replications, randomized blotch design. The experiment can be regarded as 15 weed-control strategies
where, in addition to the untreated control, six chemicals or chemical-combinations are applied (Spectrum 720 EC, Motivell Turbo D,
Stellar + Dash HC, Clio + Akris SE + Dash HC, Clio + Dash HC) in five different times (pre, early post, post and two late post) and eight
mechanical weed-control technologies were used. Hoeing took place connected to the herbicide treatments in different times: until 2-3-leave
age weedless, in 3-4-leave age hoed once, from 3-4-leave age weedless, in 6-7-leave age hoed once, from 6-7-leave age weedless, in 8-leave
age hoed once, from 8-leave age weedless.
Our results were assessed by chemical efficiency examination, maize length measurement, corncob-length and fertility examination,
Sorghum plant-number determination and yield weighing carried out in four periods. The data were evaluated by a one-factor analysis of
variance and a two-factor linear regression analysis.
Research on the allelopathic effect among the species Tanacetum vulgare and some agricultural crops105-109Views:101
The research presents the results of the allelophatic effect upon the germination and growth of plants, immediately after springing, viewed as the interaction between the species of Tanacetum vulgare sin Chrysanthemum vulgare and three other crops: wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), rape (Brassica napus ssp. oleifera L.). The experiments that were performed consisted in applying treatments with aqueous extracts obtained from the roots, leaves, stems and flowers harvested from T. vulgare plants upon the seeds of the three agricultural crops mentioned before. In all aqueous extracts, the results indicate the presence of some chemical compounds that have inhibiting allelophatic effect. The plants upon which tests were made showed great sensitivity, the results on their germination and growth being significantly negative and highly significantly negative.
The conclusions of this research sustain the idea of setting new research objectives in order to discover the chemical compounds from T. vulgare extracts that have such a stong effect and the possibilities they offer.
Effects of spray carrier quality on biological activity of terbuthylazine + mesotrione herbicide combination110-115Views:92
Field experiments were conducted to study affects of pH and hardness of spray water on efficacy of a herbicide combination (terbuthylazine + mesotrione) influenced by several pH adjusters and adjuvants in Debrecen, Hungary in 2008, 2009 and 2010. Favourable or unfavourable effects of pH and hardness of spray water could be observed under field conditions. Evaluation of weed control efficacy is suitable for examination of affects of spray water pH and hardness on herbicides. The terbuthylazine and mesotrione herbicide combination is suitable to control monocotyledonous and dicotyledonous weed species, however, significant effects of hardness and pH of spray carrier was observed only in control of monocotyledonous weeds. Certain pH adjusters (e.g. ammonium nitrate) can lessen harmful affects of water hardness effectively. Significant loss of efficacy of sensitive herbicide was found in hard water (by about 50-60%), and surfactants was not able to eliminate that harmful affect. However, biological activity was the same as in soft water with ammonium nitrate which can overcome the antagonism of salts. That pH adjuster had a more significant affect on the efficacy of the herbicide than the surfactant had in that experiment.
Changes in weed flora of basket willow (Salix viminalis L.) under different soil nutrient supply116-120Views:124
The world is in a continuous progress, as a result of which energy consumption and with this the release of gases with adverse impact show rapid increase. As a result of the survey conducted by the International Energy Agency, if the major economic powers do not initiate a change in their energy policy, the increase of energy consumption may as well reach 40 % by 2030. This increased energy demand is getting more and more difficult to fulfill with the fossil energy resources, which is to lead to an increasing significance of renewable energy resources. In Hungary, these energy resources are the best to provide with biomass growth. Biomass growth for energetic purpose can mostly be provided by energy plants, out of which “energy willow” (Salix viminalis L.) is outstanding with its high yield and with its excellent burning technology characteristics of its timber. The willow’s cropping technology is being established in our country. One of our tasks is to work out an adequate weed control plan. The professional and safe use of herbicides can increase the success of production. In our paper, we discuss the weed flora data collected on treatments applied in the different fertilizer and compost. We started our survey in 2010. We examined twelve different fertilizer and compost treated areas. The dominant weeds were: Amaranthus retroflexus, Chenopodium album, Echinochloa crus-galli among annuals; Cirsium arvense and Agropyron repens among the perennials.
Yield of herbicide tolerant sunflower hybrids due to the different herbicide treatments121-125Views:111
Sunflower is our most important oil-plant grown on the largest area in Hungary. In Europe sunflower has been grown since the 16th century. In recent years sunflower growing area is between 450-500 thousand hectares. Weed management in sunflower production is getting more and more difficult in case of annual and perennial dicotyledonous weeds, especially in dry springs. Two active ingredients, imazamox and tribenuron-methyl could be a solution for farmers for the control of these weeds in herbicide tolerant sunflower hybrids (Christensen-Reisinger 2000, Hódi-Torma 2004, Nagy et al. 2006). Most of the farmers choose the Clearfield technology and the use of tribenuron-methyl herbicides. In 2009 imazamox- (IMI) and tribenuron-methyl- (SU) tolerant sunflower hybrids were produced on 200 hectares in Hungary, of which 150 hectares was IMI, while 50 hectares was SU-hybrids. Small plot experiments were carried out to investigate the phytotoxicity of herbicides on imazamox (IMI) and tribenuron-methyl (SU) tolerant sunflower hybrids under field conditions. At harvest we measured the moisture content of achenes and average yield.
Usage of interactions among plants and pests in biogardens126-129Views:95
Experiences has been gained in the last 25 years with plant extracts, fermented juices, infusions and brews of plant origin presented in present paper. Interactions among vegetables growing in mixed cultures have been also summarized with special regard to insect repellent plants and to those interaction when the target plant’s odour is covered and the pest cannot find it. These methods – after the ecological balance of the garden has been returned – can help the growers to keep the pest density under the economic threshold. The allelopathy can be the basis of the presented results.