SNP analysis of ITS1-5.8S-ITS2 rDNA loci in modern and ancient melons (Cucumis melo)

Zoltán Szabó; Gábor Gyulai; Zoltán Tóth; László Heszky

doi:10.34101/actaagrar/27/3114

ITS (internal tanscribed spacer) profiles of the aDNA (ancient DNA) of seed remains extracted from an extinct sample recovered from the 15th century (Budapest, Hungary) were compared to 31 modern melon cultivars and landraces. An aseptic incubation followed by ITS analysis was used to exclude the exogenously and endogenously contaminated (Aspergillus) medieval seeds and to detect SNPs in ITS1-5.8S-ITS2 region of rDNA (ribosomal DNA). SNPs were observed at the 94–95 bp (GC to either RC, RS or AG) of ITS1; and at 414 bp (A-to-T substitution), 470 bp (T to Y or C), 610 bp (A to R or G) of ITS2. The results facilitate the final aim of molecular and morphological reconstructions of ancient melon tpyes.

Lejla Budai, József Racskó, Zoltán Szabó, Miklós Soltész, Ervin Farkas, Sándor Thurzó, Gábor Drén, János Nagy, Mária Dani, József Nyéki, The effect of sunburn damage on the fruit quality parameters of apple (Malus domestica cv. Idared) Acta Agraria Debreceniensis: No. 17 (2005): Special Issue

József Racskó, József Nyéki, Zoltán Szabó, Miklós Soltész, Ervin Farkas, Effect of Rootstocks on Blooming Capacity and Productivity of AppleCultivars Acta Agraria Debreceniensis: No. 15 (2004)

Zsuzsanna Galbács, Stella Molnár, Gábor Halász, Sarolta Hoffmann, Anikó Veres, Zsolt Galli, Antal Szőke, Zsófia Tóth, Katalin Pilinszky, Barnabás Wichmann, Pál Kozma, Erzsébet Kiss, László Heszky, Application of microsatellite fingerprints for pedigree analysis of Hungaricum grapevine varieties Acta Agraria Debreceniensis: No. 27 (2007)

Lajos Horváth, Gábor Gyulai, Zoltán Szabó, Richárd Lágler, Zoltán Tóth, László Heszky, Morphological diversity of current melons (Cucumis melo) compared to a medieval type Acta Agraria Debreceniensis: No. 27 (2007)

Miklós Soltész, József Nyéki, Zoltán Szabó, Local strategical tasks in the Hungarian fruit production with respect to the global economic and climatic changes Acta Agraria Debreceniensis: No. 17 (2005): Special Issue

József Nyéki, Zoltán Szabo, The past, present and future of the institute for extension and development (1996-2005) Acta Agraria Debreceniensis: No. 17 (2005): Special Issue

László Lakatos, Tibor Szabó, József Racskó, Zoltán Szabó, Miklós Soltész, József Nyéki, The effect of daytime and nighttime temperature on the cover colour of fruits in an apple gene bank Acta Agraria Debreceniensis: No. 17 (2005): Special Issue

Attila Hegedűs, Júlia Halász, Zoltán Szabó, József Nyéki, Andrzej Pedryc, How does the S-locus determining self-incompatibility in stone fruits work in self-compatible peach? Acta Agraria Debreceniensis: No. 17 (2005): Special Issue

Richárd Lágler, Gábor Gyulai, Zoltán Szabó, Zoltán Tóth, László Heszky, Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages Acta Agraria Debreceniensis: No. 27 (2007)

József Racskó, Zoltán Szabó, László Lakatos, Gábor Lőrinczy, József Nyéki, The Effect of Sunburn on Fruit Quality of cv. Idared Apple Acta Agraria Debreceniensis: No. 19 (2006)

Acta Agraria Debreceniensis

Acta Agraria Debreceniensis

No. 27 (2007)

SNP analysis of ITS1-5.8S-ITS2 rDNA loci in modern and ancient melons (Cucumis melo)

Authors

View

Keywords

How To Cite

Abstract

Most read articles by the same author(s)

Name	Gyöngyi Kovács
E-mail:	kovacsgy@agr.unideb.hu