The flowering dynamics of Ocimum basilicum L., (a common population maintained at the UHFI) was studied with the aim to create an exact and practically applicable method for definition of phenological phases.
According to our observation the development of individual flowers of the basil can be characterized by 8 distinguishabl...e phases, which must be considered for description of the actual phenological stage of a spike.
An accurate model was created for the unambiguous description of flowering process of different flowers within the spike and for the individual plant as a whole. The new flowering index formula is calculated from the number of flowers weighed by their phenological phases.
The time dependence of flowering is presented by functions fitted to values of the flowering index. The results reflected different patterns of the main inflorescence, the inflorescences formed on the side shoots in the first or in the second half of the flowering period. However, for description of the flowering process of the whole plant, a sigmoid function proved to be the appropriate model.
The accumulation process of the essential oil could be characterized by the flowering index values. They showed close correlation (r=0.964) at high probability.
The new method assures an exact definition of phenological phases in basil. Its application seems to be optimal during breeding procedures, seed production and even in production of high quality drugs, according to the requirements of the Good Agricultural Practice (Guidelines for GAP, 1998).
Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which...the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.
The flower bud development is an especially complex process from initiation to blooming. Our main objective was to analyze paradormancy; the first stage of this process in our collection of varieties in the vicinity of Budapest, in Hungary. We have analyzed three varieties with different winter hardiness. `Ceglédi bíborkajszi' is one of the m...ost frost susceptible in our collection of varieties, when the flower bud differentiation started in early August, and all flower organ initials evolved in beginning of September. The flower bud differentiation of the most winter hardy variety, `Rózsakajszi C.1406' started in the end of August, and all flower organs were noticed at middle of September. `Gönci magyar kajszi' is a medium frost hardiness apricot variety, its phenological process composes transition between two mentioned above varieties.
The seasonal dynamic of macro- and micronutrients uptake of a sweet cherry cultivar cv. 'Katalin' (Prunus avium L.) was studied according to apply different flower thinning techniques. Beside control treatment, three thinning treatments were performed: (I) thinning for I flower/inflorescence, (2) thinning for 2 flower/inflorescence, (3...) thinning for 3 flower/inflorescence. Soil examination was carried out to establish the growing conditions of orchard site. Moreover, for studying the temporal dynamic of nutrient uptake plant analytical examination was performed four times per year based on leaf collecting according to the phenological phases. It was found that the macro- and micronutrients contents of leaves were showed significant differences between treatments before ripening, at ripening and after ripening stage. It was found that thinning has influence on mineral composition of leaf. Flower removal unbalanced the equilibrium of generative and vegetative processes. The applied manual flower thinning treatment resulted improving vegetative processes like nutrient uptake and storage.
In this study, the susceptibility of 7 commercially important sour cherry cultivars to Monilinia laxa was studied. Artificial inoculation was made with M. laxa isolates, which were isolated from different woody plants. Artificial inoculation was prepared in the laboratory and in the field. In laboratory, flowers of sour cherries while in the fi...eld, the two-year old twigs were inoculated in 2006 and 2007. According to results of stigmata inoculation, there were infection ability differences among the isolates originated from five different stone fruit host. Cultivars could be sorted into two susceptibility groups. In the field, twig inoculation in 2007 was made at blossom period and in 2007 at harvest. Seven sour cherry cultivars were inoculated with 8-day-old mycelial culture of M. laxa originated from sour cherry and almond. The agressivity and pathogenicity of the two isolates were measured by the degree of floem death: Results showed that year and phenological stage considerably influenced the degree of symptoms caused by the fungus. After artificial inoculation, tissue death progression was studied by fluorescent microscope. According to results, sour cherry cultivars were sorted into disease susceptibility groups. Susceptibility orders were identical to results on stigmata inoculation.