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Identification of Rabbiteye Blueberry Cultivars (Vaccinium ashei Reade) and Analysis of Genetic Relationships Using Amplified Fragment Length Polymorphism (AFLP)
27-30.Views:109Proper cultivar identification is a requisite for commercial planting and breeding nurseries of cross-pollinated blueberry (Vaccinium ashei Reade) cultivars to insure high crop yields and optimize germplasm maintenance and utilization. Fourteen rabbiteye blueberry cultivars and three non-identified clones were screened with amplified fragment length polymorphism (AFLP) analysis with the aim of developing a fast and reliable identification technique. The selective primer pair applied (M-CTG/ E-ACC), which was previously tested, resulted in a large number of reproducible polymorphic fragments for cultivar identification. After comparison of the AFLP fingerprints, the Jaccard similarity indexes were calculated, and an UPGMA dendrogram was constructed. It was revealed that the three non-identified clones belong to the Tifblue' cultivar. Moreover, AFLP technique proved to be a fast, successful and reliable way in rabbiteye blueberry identification.
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Identification of the apple firmness: two case studies
32-35.Views:106Firmness tests were performed with peeled and entire fruits of Elstar and Jonagold apple cultivars for identification and comparison. The normal distribution of the tested population was acceptable (level: 95%). The green and the red sides did not show differences within the cultivar but they were different in firmness. There was not significant difference between the flesh tissue firmness values, however the firmness of the entire (not peeled) fruits was different. This result was caused by the effect of the peel. The variability of the firmness with Jonagold was caused by the peel, but such a result was not found with Elstar. The test of the peel effect would be interesting with different cultivars and a sequence according to the firmness can be estimated.
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Determination of (in)compatibility genotypes of Hungarian sweet cherry (Prunus avium L.) accessions by PCR based methods
37-42.Views:163Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.
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Molecular analysis of strawberry cultivars using RAPD, AP-PCR and STS markers
24-28.Views:121Seventeen strawberry (Fragaria x ananassa Duch.) cultivars representing the national list of Hungary, were subjected to RAPD, AP—PCR and STS analysis. Of the 31 decamer and oligomer primers tested 26 primers produced polymorphic patterns. 45 polymorphic fragments were analysed, ranging between 200-2800 by in size. Based on the data, similarity coefficients (Jaccard index and Simple matching coefficient) were calculated, and dendrograms were constructed using the unweighted pair group method of arithmetic averages (UPGMA). The dendrograms only partly reflect the known pedigree data. Specific RAPD markers were identified for cultivars F5, Pocahontas and Rabunda.
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Fire blight (Erwinia amylovora) resistance in apple varieties associated with molecular markers
53-57.Views:223The invasive bacterial disease fire blight, caused by Erwinia amylovora has the potential to destroy fruit tree orchards all over Europe. Effective plant protection methods are lacking in many countries, highlighting the increasing importance placed on identification of germplasm with heritable disease resistance. Recent l y. a promising QTL (quantitative trait locus) was identified on linkage group 7 in the apple cultivar 'Fiesta· which is derived from ·cox's Orange Pippin' . I n the present study, 144 Swedish and foreign apple cultivars were analysed with the SCAR markers AE I 0-375 and GE-8019. which flank-. this QTL. Twenty-nine of the analysed cultivars had both markers. 78 had either AE I 0-375 or GE-8019, and 37 cultivars did not carry an) of the two markers. Seventeen cultivars. 7 with both markers and I 0 not having either of the two markers, were then inocoluted with the bacterium in a 4uaran1i ne greenhouse test. Cultivars carrying both DNA markers were significantly less susceptible than cultivars lacking the markers, P<0.001. Cultivars that were most resistant had both markers and had 'Cox· in their pedigree. Unrelated cultivars with the markers may still lack the QTL.
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Molecular characterization of apricot (Prunus armeniaca L.) cultivars using cross species SSR amplification with peach primers
53-57.Views:200Apricot takes an important place in Hungarian fruit production. Considering morphological characteristics of apricots it was concluded that the genetics background of European cultivars is very limited. Molecular markers and their use for genotyping have revolutionized the identification of cultivars. In a classic apricot breeding program, it is important to be able to establish unique DNA profiles of selections to identify them unambiguously and to determine their genetic relationship. Presently SSR is far the most frequently performed technique for genetic diversity studies. In this study there were used peach and apricot primer pairs from four different sources in order to examine microsatellite polymorphism among cultivars and investigate relationships among them. The possibility of cross species amplification among different Prunus species using SSR primers allowed us to use primers developed in peach to study genetic diversity in apricot. In this work, 90% of the primers used were able to amplify SSRs in apricot and more than half of them were polymorphic. With the 10 primer pairs utilized were proven to be sufficient to set unique fingerprint for several cultivars studied. The obtained dendrogram classified of the 45 cultivars included in this study into two major groups and several subgroups.