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Attempting Regeneration from Cultured Cotyledons and Plant Regeneration from Cotyledonary Nodes in Common Bean (Phaseolus vulgaris L.)
57-60.Views:263Dry seeds from two cultivars of common bean (Phaseolus vulgaris L.) were germinated on sterile cotton and sterile deionized distilled water. Cotyledonary node tissue of seedlings were cultured on Murashige and Skoog(MS)-based media supplemented with different combination of N6-benzyl-aminopurine (BAP) and indole-3-acetic acid (IAA), and benzyladenine (BA) and a-naphthaleneacetic acid (NAA). The results revealed that the regeneration percent and the average number of buds and shoots per explant were influenced by the type of explants and exogeneously added hormones. Multiple shoot induction on dry bean cotyledonary node that contain 4-5 mm from cotyledons and hypocotyl on a medium containing full concentration of MS inorganic salts supplemented with 0.5mg/1 BA and 0.1mg/1 NAA was feasible and the method can be applied in transformation experiments.
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Evaluation of morphological parameters and bioactive compounds in different varieties of beetroot (Beta vulgaris L. ssp. esculenta GURKE var. rubra L.)
31-35.Views:672Beetroot consumption based on pickled beets generally in Hungary which is due to the higher yield from second crop harvested in autumn and processed by manufacturing industry. Researches of the past years confirmed its favourable nutritional-physiological effects on human body so demands, as for fresh salad, also increased. The trial aimed at testing the interaction of varieties on quality parameters and in the same time suggestions are made how to use different varieties of beetroot according to its quality. Morphological and sensory evaluations were examined on 10 varieties of beetroot harvested in autumn. The regular spherical shape can reduce the refining loss during the processing of beetroot which is beneficial for the manufacturing industry. In our trial the root shape of Libero, Mona Lisa and Rubin varieties approached most the regular spherical shape (diameter/length – 1.0) which is favoured by not only processing industry but also fresh market. The highest red pigment content (betanin) was observed in Mona Lisa, Akela and Cylindra (34.58–47.66 mg/100 g). A similar trend could be observed in yellow pigments (vulgaxanthins) which proves the close correlation between the quantities of the two pigments (r=0.898). Highest total polyphenol (77.13–83.37 mg GAE/100g) and flavonoid (21.73–22.73 mg CE/100g) contents were detected in Akela, Mona Lisa and Bonel. These varieties are favourable for fresh salad and they can satisfy processing requirements also. Highest water soluble solids content was found in Akela (7.15%). In our conditions nitrate (NO3-N) values below 900 mg/kg were examined in all of the varieties which is favourable in the case of beetroot.
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Concentration of ‘Oblachinska’ sour cherry juice using osmotic distillation
31-34.Views:926Sour cherry is one of the most famous and characteristic Hungarian fruit what is also commonly used in food production as a colour additive. The sour cherry cultivar, ‘Oblachinska’ was shown to be a rich source of antioxidant compounds, including mainly anthocyanins. Valuable compounds in fruit juice – vitamins, polyphyenols etc. –are heat-sensitive molecules, which should be taken into account during the process of concentration to prevent degradation. Osmotic distillation seems a suitable option to product high quality sour cherry juice because this process does not require high temperature or pressure. Raw juice with approximately 15°Brix was used for the experiment and tried to concentrate up to 60° Brix where the water activity low enough to inhibit the microbiological deterioration. Before and after the process, TPC (total phenolic compounds) and FRAP (ferric reducing antioxidant power) was measured using spectrophotometric methods to determine the effect of the osmotic distillation. Our results point out that osmotic distillation is a promising method to concentrate sour cherry juice and prevent the loss of valuable compounds.
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High antioxidant - and anthocyanin contents of sour cherry cultivars may benefit the human health: international and Hungarian achievements on phytochemicals
45-47.Views:1017Evidence suggests that a diet with high fruit and vegetable consumption may decrease the risk of chronic diseases, such as cardiovascular diseases and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proved that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. According to the published data, the most important biological effects of sour cherry are connected — directly or indirectly — to their endogenous antioxidant behaviour as well as to their specific pattern of anthocyanin components. In the present work, we measured the total antioxidant capacity of some Hungarian sour cherry varieties in combination with their anthocyanin-, and vitamin-C content. In 2003, twelve clones were selected and grafted from a local sour cherry population called "Bosnyák" sour cherry grown in small home gardens and farms of the village Csengod (Great-Plain Region, South Hungary). Other Hungarian sour cherry varieties, i.e. cv. Újfehértói fürtos, cv. Érdi bőterrnő, cv. Debreceni bőterrnő, cv. Csengődi and cv. Kántorjánosi served as a control.
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Contributions to the resumption of growth in ecodormant buds of apple
35-40.Views:235The resumption of development in ecodormant buds in terms of establishing a functional vascular connection between the inflorescence primordia and spur tissues in apple trees was investigated. Differentiation of the xylem elements could be observed first in the pedicel of the flower primordium, in the middle of January. Much later (at the beginning of April) there were mature xylem vessels in the wall of the receptacle and, merely a procambial strand for the ovule primordium which was at this time an undifferentiated protrusion of meristematic cells, only. As for phenological development of buds incubated at a temperature of 20 °C, it was the slowest in buds sampled in January, faster in buds sampled in the middle of February and, buds from the middle of March responded very quickly. The function of temperatures needed both for xylem differentiation and for the flower primordium to achieve maturity is pointed out. The nature of frost damage in vessel elements, as well the relationship between chilling requirement and growth features of apple cultivars will be discussed.
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Effect of postharvest sodium benzoate treatment on some fruit parameters of two organic apple cultivars
35-37.Views:746The aim of this study was to investigate the effect of postharvest sodium-benzoate treatment on some fruit parameters of two organic apple cultivars (’Topaz’ and ’Florina’). Assessments were made at three times during storage: 17 November 2019, 20 December 2019 and 23 January, 2020. During every assessment dates, each fruit was observed separately, and determined the proportion of i) healthy fruits (%), ii) post-harvest fruit rot diseases iii) fruits with mechanical injury (%), iv) fruit with russetting (%), v) damage of codling moth and vi) Ca-deficiency symptoms. In addition, fruit weight (g) was measured at each assessment date. Sodium benzoate reduced the fruit decay and the proportion of healthy fruit was higher in this treatment compared to water treated fruit. This effect could be seen in all assessment dates and on both cultivars. Effects on other parameters were various according to cultivar and assessment dates. Loss of fruit weight was similar in both treatments and cultivars compared to control treatment.
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Response of alternate bearing of “Badamy sefid” Pistachio cultivar to foliar application of some macro and micro elements
109-112.Views:316Response of “Badamy sefid” Pistachio trees to spraying of fertilizer was studied over two years (2006–2007). The boron and zinc fertilizer were used just on swelling time of female flower bud in compare to no fertilization (control). Also nutritional solution combined of nitrogen (400 ppm), phosphorus (P2O5) (380 ppm), potasium (K2O) (520 ppm), Fe (5 ppm), Cu (2 ppm), Zn (2 ppm), Mn (2 ppm) in 1000 liter water per hectare sprayed at first week of May, third week of June and July). The concentration of some macro and micro elements in flower bud and in leaves, also productivity (g/shoot sectional area) mean nut weight, fresh weight of nut per cm2 branch cross sectional area, blanks nuts %, non-split nut %, new shoot growth length after cessation of growth and remained flower bud (%) at the next spring were recorded for the different treatments. The results showed that boron and zinc concentration increased in the bud of sprayed trees in compare to control. Application of nutritional solution decreased the flower bud abscission, also resulted in increased vegetative growth, nut weight, and productivity of trees. In conclusion, spraying of fertilizers in the suitable time to the pistachio trees might be a useful method in decreasing flower bud abscission and mitigating the alternate bearing.
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The utilisation of nutrient elements in soilless cultures of vegetable production
47-49.Views:321Vegetable production in greenhouses may impair the ecological balance of the environment substantially as far as being uncontrolled. Soilless cultures especially should be handled thoughtfully. A fraction of the nutrients administered, more than 25-30%, is doomed to be lost in an open system, and the resulting ecological risk is accompanied with increasing costs of the production. Experiments have been conducted with the purpose of estimate the amount of nutrients involved. According to the results, as a mean, 30-80 per cent of the main nutrients was utilised. The rate of nutrient utilisation is influenced by the plant species involved as well as by the circumstances of production. One of its most important components is the irrigation, which determines the amount of overflow and of its salt content.
