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Security of growing habits and bud formation of German sour cherry varieties
45-48.Views:686Intensive sour cherry production is concerned to find the most productive varieties under special growing conditions. High planting density, adapted to manual picking or on the other hand to mechanical harvesting. Almost as important is the prolongation of the ripening season by enlarging the choice of varieties. Unfortunately, three of our four leading commercial varieties are ripe almost at the same date.
Therefore, new varieties ought to be examined thoroughly. In the Pallag Experimental Station of the Debrecen University, five European varieties have been grown (’Schattenmorelle’, ’PI-SA 12,100’, ’Jade’, ’Gerema’, ’Achat’) and a Hungarian one, ’Debreceni bôtermô’ used as a check for the experiments to compare their growing and yielding habits in 2010. The plantation was three years old, standing on Prunus mahaleb rootstocks, in high density (5 x 2 m) and trained to slender spindle crowns. The results are proving that some of the varieties in
question are suitable to prolong the harvesting season. Growing habits and yields of the varieties related to the variety ’Debreceni bôtermô’ were similar or even better as ’Jade’, ’Gerema’ and ’Achat’. The ’Jade’ excelled with its vitality and ’Gerema’ with its generative character. Further studies are expected to prove the utility of
one of them at least to enlarge the ripening season on the fruit market. -
Evaluation of vermicompost application and stress of dehydration on mullein medicinal plants
69-77.Views:339The use of organic fertilizers is one of the suitable solutions in the organic production of medicinal plants due to its good effect in improving soil properties, reducing environmental effects, and better plant growth. To investigate the effect of vermicompost organic fertilizer application and water stress on some morphological and physiological traits of the Mullein medicinal plant, research was conducted at Isfahan Azad University in the form of split plots in the form of a randomized complete block design with 4 replications. The test factors included the application of vermicompost organic fertilizer at three levels of 0, 4, and 8 kg per square meter of soil and water stress at two levels of normal irrigation and irrigation at the time of 50% of the soil's agricultural capacity as the main treatment in research farm conditions. The results of the experiment showed that the application of vermicompost organic fertilizer and water stress improved the morphological and physiological characteristics of the Mullein medicinal plant compared to the control, i.e. no application of organic fertilizers. The results showed that the highest number of secondary branches, number of flowers of the secondary stem, diameter of flowering stem, the diameter of flower, and fresh weight of shoot in the Mullein medicinal plant were obtained by applying vermicompost organic fertilizer at the rate of 4 and 8 kg of soil. The application of vermicompost organic fertilizer at the rate of 8 kg in the soil increased flavonoids compared to the non-use of vermicompost treatment. However, to improve the vegetative growth and increase the reproductive efficiency of the Mullein plant and reduce production costs, the use of vermicompost organic fertilizer is recommended at the rate of 4 and 8 kg of soil, respectively.
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In vitro regeneration from cotyledons of watermelon
96-98.Views:285Cotyledonary segments of five different genotypes of watermelon were used to induce organogenesis. Five different hormone combinations were applied to enhance the induction of shoot formation on the surface of the segments. The phases of organogenesis were followed with light and scanning electron microscope. Shoots were obtained after four weeks, then the shoots were transferred to hormone free medium for root induction.
This method of regeneration can be applied in transformation experiments. GUS histochemical assay was made to check the expected success of using Agrobacterium for the transformation.
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Estimating of water consumption of cherry trees
15-17.Views:398Despite of its importance there is no exact information on water use of new scion/rootstock composite trees, which would be needed to optimized irrigation. Our research purpose is to define exact water-demand of different rootstock/scion composite trees, calculating seasonal weather changes and by using the results decrease irrigation costs. The investigations are carried out in Soroksár, at the Experimental Farm of Corvinus University of Budapest in May 2008. From among the investigated trees two are budded onto Prunus mahuleb `Érdi V' seedlings, two on "Korponay' seedlings. The sapflow measurements are carried out using Dynamax Flow 32 equipment with Dynagage trunk sensors. The first daily maximum of sapflow was around 10:00 a.m. (2.5 kghour I), the second maximum was always between 14:00-15:00 p.m. (2 kgday- I). Comparing to the very intensive morning water uptake by 20:00 p.m. the water flow slowly reached the minimal level. Significant differences can be seen on rootstocks: trees on `Korponay' rootstock always showed more intensive sapflow and a higher morning peak than trees on `Árdi V'. But later during the day they have the same run.Based on our results the water quantity transpired only by the trees reached in May 86-104 mm, while the precipitation was only 42.4 mm. This means a 40-60 mm deficit in the orchard, which should have been supplied by irrigation despite of the satisfying horticultural performance of the orchard. In the first half of the month beside the steady vapor pressure deficit the shoot and leaf surface growth could cause the increased sapflow.
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The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon
46-49.Views:371Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.
