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Optimization of sample preparation for determination of antioxidant parameters from one grape berry
93-97.Views:441Berry quality is an important issue in wineprocessing, however evaluating characteristics in a single berry level is very complex. The Phenolic compound analysis is more challenging because of the limited amount of material and a mixture of skin, pulp and seed. No clear protocol exists for evaluating these compounds from single berries. The aim of our investigation was to develop such a protocol. Single whole grape berries samples were homogenized in 20 replicates by 5 different methods. The most effective method was when berry was placed in a mortar and was crushed with a pestle under liquid nitrogen until seeds were clearly visible. Seeds were then taken from the mortar and crushed between two stainless steel plates with a hammer, and returned to the mortar without residues. Homogenization continued until the sample had a powdery appearance. The homogenized samples were taken for further analysis such as total polyphenols, total anthocyanin contents and total antioxidant capacity. Our results demonstrate that single berries could be processed and that the homogenates were suitable for taking chemically uniform subsamples.
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Eicosapentaenoic acid production by Phaeodactylum tricornutum under different culture condition
133-136.Views:224Phaeodactylum tricornutum UTEX 640 strain of microalgae was screened under different culture conditions for their capacity to produce eicosapentaenoic acid (EPA) the most abundant polyunsaturated fatty acid (PUFA). In our experiments, the effect of sodium chlorid, nitrogen source, phosphate, initial pH, as well as the CO2 content of the medium on production of the eicosapentaenoic acid (EPA) by P. tricornutum were investigated. The EPA content of biomass was enhanced by the low pH of the medium, with increased concentrations of B17 vitamin and nitrate, and also with decreasing concentrations of sodium chlorid. The EPA is most likely associated with polar (membran) lipids and the role of EPA appears to be involved with membran permeability in microalge. The synthesis of phospholipids, enhances the EPA content of the cells, as expected. The maximum EPA yields were observed under optimum culture condition 43 — 48 mg/g of dry cell weight.
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Effect of Foliar Nutrition on Post-Harvest of Onion Seed under Sandy Soil and Saline Irrigation Water Conditions
85-92Views:420Foliar application has been determined to be an effective nutrients delivery strategy in vegetable and fruits. The enhancement of vegetable and fruit yields affected by foliar nutrients application has been recognized in previously conducted studies with perennial tree crops. The efficiency of foliar nutrition is dependent on soil, climate, fertilizer and the amount of nitrogen used. There is no sufficient information concerning cooperation of foliar nutrition with all nutrients form as well as the rates of these nutrients fertilization in vegetable
and fruit crops. Two successive winter seasons of 2008/2009 and 2009/2010 were conducted under sandy soil conditions to study the effect of spraying with 12 commercial compounds on inflorescences diameter, flower stalk length, number of seed stem /plant, weight of 1000 seed, germination percentage, seed yield, moisture content, catalase , peroxidase activity and malondialdehyde content of onion seeds. The plants
sprayed with union Zn, union Mn, union feer, shams k, elga 600, boron, and amino x had the highest vegetative growth parameter, germination percent and enzyme activity. The plants sprayed with union Zn, union feer, shams K, magnesium, caboron, hummer and amino X had the highest seed yield ha-1. The seeds were stored for one year to study the effect of different commercial compounds and storage temperatures on germination, moisture content and change in antioxidant enzymes activities of onion seeds during the storage period. Storage at cold temperature showed higher germination percent, moisture content and lower malondialdehyde content than storage at room temperature. The treatment with union Zn, union feer, union Mn, boron, elga 600, caboron, amica, hummer and amino x had the highest germination percent. -
Composted and natural organic materials as potential peat-substituting media in green pepper growing
31-35.Views:249Peat is the most favourable and usable medium in vegetable and ornamental plant forcing but because of the intensive exploitation peat resources decreased significantly all around the world. As peat-reserves run out the use of pine bark, composts and other organic materials spread in horticultural growing. In this study we compared the suitability of peat-based media to pine bark and two types of composts. We examined the effect of different organic materials on the growth and yield of green pepper (Capsicum annum L., variety Danubia). We found that the most developed plants were grown in peat-based media and pine bark. The average fruit weight was the highest in low moor-high moor peat mixture and pine bark. The plants which were grown in composts fell short of our expectations.
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The effects of foliar nutrition containing various macro and microelements on the growth and development of young grafted walnut (Juglans regia L.) plants
33-37.Views:331The Stopgril liquido (S), a foliar fertilizer containing nitrogen, calcium and magnesium was used to improve the spring and the summer growth of grafted walnut plants in the second year of cultivation in the nursery. To accomplish early termination of vegetative growth and good lignification, the composed mineral fertilizer Hascon M 10 AD (H) containing phosphorous, potassium and microelements B, Mn and Mo was applied on the same plants. Four treatments (S, H, SH, C) were applied three times in a growing season. Their effects on the height and the basal diameter of the plants in the nursery depended on the starting height of the plants and the time of application. During the first growth period in the orchard, the number of developed buds on the plant, the circumference of the plants and their height were influenced by the treatment in the previous year in the nursery. According to the results obtained through the investigation, three applications of Stopgril + Hascon in the nursery per year can be recommended in order to obtain high enough and well lignified two-year-old grafted walnut plants.
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Effect of different biochar on acidic soil, growth, and nutritional status of oil palm (Elaeis guineensis Jacq.) under the nursery conditionrsery condition.
88-94.Views:450Biochar is a widely known organic amendment that enhances plant growth and productivity through improving soil condition and fertility. However, plant and soil response differently to various types of biochar. Therefore, the major objective of the present study was to determine the effect of two types of biochar; vetiver grass biochar (VGB) and pineapple leaf biochar (PLB) on selected soil properties and understand their role in improving plant growth and nutritional status. Net house experimental study with oil palm (Elaeis guineensis Jacq.) seedlings was conducted at the farm unit of UiTM Samarahan campus for 240 days between November 2022 and July 2023 in order to understand the impact of different application rates (25, 50, 75, and 100%) of vetiver grass biochar and pineapple leaf biochar on selected soil properties including pH, electrical conductivity (EC), nitrogen, phosphorus, and potassium content, plant growth parameters including plant height, bole diameter, leaf number, SPAD chlorophyll content, plant dry weight, and plant’s NPK content. The experimental design used is randomized complete block design (RCBD) with 8 treatments, 3 replicates, and each replicate has 6 plants. The analysis of variance (ANOVA) and DMRT were used to analyze the collected data from soil and plant parameters. The analysis process was accomplished using SAS package (P<0.05): version 9.4 (SAS, 2013). Based on the results, vetiver grass biochar and pineapple leaf biochar enhanced soil pH, EC, and NPK content. They also improved the growth and nutritional status of oil palm seedlings. Soil enhancement was the best with 100% pineapple leaf biochar. In addition, the highest mean value of plant height, bole diameter, leaf number, SPAD chlorophyll content, plant dry weight, and plant’s NPK content was observed in plants treated with 100% pineapple leaf biochar. Therefore, vetiver grass biochar and pineapple leaf biochar have the potential to enhance acidic and poor-fertile soil, and improve oil palm seedlings growth and nutritional status.
