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Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures
15-23.Views:407The effects of kanamycin and hygromycin-based selection and co-culture temperature ranging from 22 to 28 °C upon eggplant transformation efficiency were evaluated. Both morphogenic pathways, somatic embryogenesis and organogenesis, were adopted using cotiledonary and hypocotyl explants, respectively. Somatic embryos were recovered in the presence of both antibiotics, although lesser escapes were observed in hygromycin-supplemented medium. Indeed, selection provided by this antibiotic was more efficient compared to kanamycin, nevertheless, shoot regeneration was not observed with hygromycin. Significant difference on the frequency of cotiledonary explants displaying callus (FEC) was observed as embryogenesis was concerned, although a higher number of embryos was observed in hygromycin selective media. The frequency of explants presenting callus (FEC), embryos (FEE) and shoots or buds (FERG) did not differ statistically for the tested co-culture temperatures, although higher regenerant number was observed at 24 °C.
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The effect of wear of petrol engine power brush cutters on their vibration exposure
37-44.Views:311The matter of noise and vibration exposure has an accentuated emphasis in the environmental protection policy of the European Union. In practice, the focus is on the evaluation of noise -strain. The evaluation on human beings' vibration-strain is in focus in recent years. At the Technical Department in Corvinus University of Budapest Faculty of Horticultural Sciences we evaluate in PhD training form the possible ways of alleviation and revealance of noise and vibration-strain in gardening devices and machineries. According to the latest development in gardening culture, small gardening machines are more and more widespread. There is a law in Hungary for the compulsory grass-cutting, especially on 'ragweed' (Ambrosia elatior). Along highways, public territories, parks workmen at least six months are in charge to terminate these weed-crops meanwhile they are endangered by serious harmful effects. The operators of hand-held power tools are exposed comprehensive levels of hand-arm vibration at the tool-hand interface. Many studies indicate that extended exposure to mechanical vibration can induce degeneration of the vascular and sensioneural systems in the hand called hand-arm vibration syndrome (HAVS). The precise mechanism for the initiation and development of HAVS is unclear to date. Measurement and risk assessment of hand-transmitted vibration is mostly based on the guidelines and dose-response relationship provided in the ISO-5349 standard. These guidelines suggest that the magnitude, frequency, direction and duration of vibration exposure are the most important variables for the risk assessment of hand-arm vibration. This current study is focusing on brush cutters owned by one of the biggest caretaker company. These machines have similar brand and age but different in usage time. We inquired seven brush cutters through two years. The manufacturer repaid the vibration level of the new machines, but they doesn't examine, whether extent of the vibration emission is changing for the proper use. We wanted to know that are there any differences between the values of vibration specification after certain time of usage. Is it possible to monitor the alternate movements on graphs according to the vibration sciences? By Ill octave band frequency and FFT analysis we can point at that these brush cutters can show differences in critical frequencies. These biases can influence the hand-arm system and its vibration-strain burdening.
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The effect of modified bacterial virulence to host-pathogen relationship (Phaseolus vulgaris L. Pseudomonas savastanoi pv. phaseolicola)
53-56.Views:550The Pseudomonas savastanoi pv. phaseolicola is one of the most expressive biogen stressors of the bean (Phaseolus vulgaris L.) in Hungary. The chemical and agrotechnological defence is inefficient, so breeding is the only workable way. The conventional cultivars are susceptible to PS while most of the new industrial varieties have genetic resistance to the pathogen. The genetic background of resistance is, however, a complex system in the bean. Leaf resistance is a monogenic system, but this gene is not expressed in juvenile stage of the host. The pathogen species can be divided into different races. After inoculation with virulent strains, typical symptoms appeared on the leaves. To understand the details of host-pathogen relationships, there were carried out experiments using bacterial strains with altered virulence. Six transposon mutants of the PS were tested. Our main objective was to test these modified bacterial strains on bean cultivars of known genetic background. First we analysed the symptoms, and then the correlation between the symptoms and the multiplication of mutant bacteria. Three cultivars (Cherokee, Inka and Főnix) were tested.
The infection by the virulent PS isolate produced typical symptoms on the three cultivars tested. Mutant bacteria (except strain 756) did not cause any significant symptoms on the hosts. The mutant 756 induced visible symptoms on the cultivars Cherokee and Inka. On Cherokee there were small watersoaked lesions, and HR (hypersensitivity reaction) was detected on Inka, but this was restricted to some cells only (mikro HR). The rate of multiplication of the wild type strain was much higher than the multiplication of the mutants. Bacteria were detected in the cotyledons and primordial leaf, but there is not any substantial number of bacteria in leaves, except for strains 757, 1212 and 1213. The rate of multiplication of strain 756 was intermediate. These, and other experiments can help to understand the genetic background of resistance and the host-pathogen relationship in the Pseudomonas-bean pathosystem.
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Ecotoxicology of Copper in Horticultural Soils: A Review
7-18.Views:660Nowadays, the world is facing the problem of environmental pollution because of the increase of man’s needs requires development in life activities, progress industrialization, transportation tools, enhancement of agriculture and exploitation of natural resources. Soil and water resources are extremely exposed to pollution from different aspects. Agrochemicals in particular, have created severe problems, since they release thousands of chemicals to the environment. Several studies on the effect of environmental pollutants on agroecosystem have been carried out. On the other hand, the importance of trace elements as environmental pollutants is well known and well documented in literature. Cu contamination to agricultural soils has been accelerated due to its wide and repeated use in agriculture and horticulture as fertilizers or fungicides to protect vines, citrus trees, and other fruit crops against fungus diseases. Applied Cu from different agrochemical sources to agroenvironment may be adsorbed and are transported to the groundwater table and pollute it besides polluting the soils. The use of Cu-based fungicides in vineyard soils is widely documented worldwide. It has been found that many countries contain concentrations in excess of 100 mg kg−1. Importance of study of transport of Cu arises due to the fact that Cu is absorbed in soils and also reaches the groundwater table, thus polluting both soil and ground water. It is often more important to be able to estimate the mobile fraction, the readily soluble fraction, the exchangeable fraction, or the plant available fraction of Cu content of a soil as a more direct indication of the likelihood of deleterious or toxic effects on soils and groundwater. Therefore, the aim of present work was to highlight the behavior and ecotoxicological effects of copper on horticultural soils.
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General defense system in the plant kingdom
79-84.Views:275The goal of plant breeders is to improve the resistance of crops against virus, bacterium and fungus pathogens was easiest to achieve by selection for phenotypes displaying the hypersensitive reaction. The resistant plant of that type keeps its health by preventing or delaying the systemization of the pathogen by destruction of cells and tissues of variable size or amputation of the contaminated organs. The faster the reaction of the host plant is the more efficient and economical is the defense, since the extent of tissue destruction decreases proportionally with the speed of reaction.
During a breeding program for resistance carried out on several plant species, mainly vegetables over thirty years, also an alternative defense reaction has been experienced, which fundamentally differs from the hypersensitive reaction. In that reaction the cells and tissues of the host plant being exposed to the pathogen do not die, on the contrary they hinder systemization of the pathogen by tissue thickening. An additional significant difference is that on the contrary to hypersensitive reaction this reaction is less host- or pathogen-specific and works excellently even at high temperature (over 40 °C).
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Changes in the Carotene Content of Nantes Type Carrots during Storage
72-75.Views:213Changes in carotene content occurring during winter storage in Nantes type carrot hybrids grown on soils of different quality (sand and loam) have been investigated. The carotene content of the samples grown in a rainy year (1999) was lower than that of the samples grown in a dry year (2000). Lower carotene content was found in carrots produced on sandy soil (Szatymaz) than on loam soil (Tordas). The increase in carotene content (2-11 mg1100 g) measured at the end of winter storage was related to the season's character.
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Recent findings of the tree fruit self-incompatibility studies
7-15.Views:467This review endeavours to collect all recent and substantial contributions to the quickly deepening fields of tree fruit self-incompatibility studies and hence updating previously published reviews. Studies carried out to discover the molecular basis of gametophytic self-incompatibility are summarized and a newly described model for the solanaceous plants is also outlined. We describe recent findings in all economically important fruit tree crops involving apple, European pear, sweet and sour cherries, almond, Japanese plum, sloe, Japanese apricot, European apricot and peach. Additional DNA sequences are now available for both the pistil and pollen component genes in several species and their molecular, evolutionary or economic implications are discussed in the light of the fruit setting behaviour.
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The brown rot fungi of fruit crops (Monilinia spp.): II. Important features of their epidemiology (Review paper)
17-33.Views:569Plant disease epidemiology provides the key to both a better understanding of the nature of a disease and the most effective approach to disease control. Brown rot fungi (Monilinia spp.) cause mainly fruit rot, blossom blight and stem canker which results in considerable yield losses both in the field and in the storage place. In order to provide a better disease control strategy, all aspects of brown rot fungi epidemiology are discribed and discussed in the second part of this review. The general disease cycle of Monilinia fructigena„M. laxa, M. fructicola and Monilia polystroma is described. After such environmental and biological factors are presented which influence the development of hyphae, mycelium, conidia, stroma and apothecial formation. Factors affecting the ability of brown rot fungi to survive are also demonstrated. Then spatio-temporal dynamics of brown rot fungi are discussed. In the last two parts, the epidemiology of brown rot fungi was related to disease warning models and some aspects of disease management.
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Effects of excessive weather on the micro-climate of apple plantations under the hail protection nets
81-85.Views:26146The general utilisation of hail nets is spreading all over the world and in Hungary too. Hail nets are at present the only tool, which
is able to prevent heavy hail damage in agricultural crops. Besides its numerous advantages, there are also deleterious side effects, e.g. it
changes the microclimate of the crop stand. Our study aimed to observe the differences, which characterise the physical status of crop stands
under the protection of hail nets compared with the stands in open air under various meteorological conditions: high or low temperature, calm
or windy atmosphere and their possible combinations. The results indicated that on hot and calm days the difference may attain 3–4° C. On
cool summer days, the difference was only 1.5 ºC. The hail net influences the relative humidity of the air, which means on ho summer days
often 7-8% differences. Conditions of radiation may also differ conspicuously. Measurements prove the reduced global radiation on sunny
summer days to 70%. This impairs as a rule the development of fruits reducing their quality and also its quantitative traits. Sunburn is, on the
other hand, significantly prevented by shadow. -
Studies on the Tobamovirus resistance of the pepper (Capsicum annuum L.) cultivar Greygo
71-75.Views:247Resistance of the Hungarian pepper (Capsicum annuum L.) cultivar "Gre.ygo" to Tohamoviruses has been investigated. All plants of the population of Greygo proved to be resistant to tobacco mosaic and tomato mosaic viruses (TMV, ToMV), both represent the pepper pathotypes Po of Tohamoviruses. Individuals of Greygo, however, were found to be susceptible to pathotypes P12 and P123 of pepper mild mottle virus (PMMV). When inoculated with the XM isolate of dulcamara yellow fleck virus (DYFV, pathotype P1) the population of Greygo segregated in resistant and susceptible plants. These results as well as inoculations of the progenies of three TMV resistant plants clearly showed, that besides the resistance allele Li the cultivar Greygo possesses also an another allele. This allele, provisionally marked by L2g behaves like to the allele L2 characteristic to Capsicum frutescens cv. . Tabasco. Determination of the identity of the allele L2g to the allele L2 needs further genetic and pathological informations. Relations between the Tohamoviruses pathogenic to pepper and the alleles of the resistance gene L are outlined for the discussion.
