Plant breeding uses androgenesis-derived dihaploids for faster breeding. A small percentage of autotetraploids are also produced during the production of dihaploids. This paper presents a microplot field study of these tetraploids. The experiment used 6–6 diploid-tetraploid pairs, which are the results of three different combinations. In addition to phenological data (emergence, plant height, developmental stage on BBCH scale), yield and its components (panicle length, number of filled grains, number of unfilled grains, fertility) were also determined. The uniform vegetative development of tetraploids did not differ from that of diploids. However, they have a longer cropping period due to the prolongation of the reproductive phase. The least time delay was observed in line of 1087/8/35T, making it one of the shortest growing time tetraploid lines. In addition, this genotype has the highest number of filled grains and the second lowest unfilled grains, causing a very high fertility (~60%). This value is very encouraging, as conventional tetraploid lines have a fertility of 50%, while the new neo-tetraploid and PMeS lines have fertility in the range of 68–80%. With the expansion of experiments, hopefully, the genotypes with higher fertility will be identified.