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Spatially Continuous GIS Analysis of Sampling Points Based on Yield and Quality Analysis of Sugar Beet (Beta vulgaris L.)
56-61Views:290The homogeneity of a study area of 20x20 m used for beetroot production in North-West Hungary was analysed with geo-statistical methods on the basis of measured plant and soil parameters. Based on variogram calculations (Equation 1 and 2), the yield surface showed homogeneity in North-South direction. Considering the results, decrease of sampling distance to 17 m can be suggested. The direction of the variability of yield (Figure 1) could be modelled with a direction variogram based on analysis of the variogram surface. In the study, developed methodological processes are presented for the analysis of spatial relationship between measured production and soil parameters. 5 spatial evaluation methods for yield surface were compared (Table 1). On the basis of the analysed methods, it can be stated that different methods (LP, RBF) should be used when the reasons for locally extreme yields are in focus than in case when the yield surface of the whole area is estimated (IDW, GP). Using adequate parameters the kriging method is applicable for both functions. Similarly to the results of an ordinary Pearson correlation analysis, spatial correlation analysis was shown using soil pH and Cu concentration data. The results of cross variogram analysis (Equation 2) and the North-South direction of the variogram surface showed negative correlation (Figure 3). Based on simulation calculations, decrease of 30% in sampling points resulted in increase of 12% in error for the total sample number considering Cu concentration. The method provides a tool to decrease the cost of sampling and sample analyses of spatially correlating features, and to increase the reliability of spatial estimation using a better sampling strategy with the same sample number.
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Angle-count sampling method for estimating forest stand volume – a practical approach
99-102Views:354Point sampling, which is also known as angle-count sampling (ACS), can be considered an efficient way of estimating the basal area and volume of forest stands. It is possible to use it in forest management: providing more accurate estimates (precision <10%) of site and stand characteristics needed for management planning. 20 black locust (Robinina pseudoacacia L.) stands were selected at final cutting age to determine the regeneration criteria based on their total volume. It was verified that at P=5% there was no difference between the main volume values of stands indicated in the relevant forest plans as well as calculated by the ACS method.
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Comparative study of wold and lowland situated small watercourses from fishbiological aspect
54-59Views:123Fishbiological research was held on 10 small watercourses, especially on the streams of woldy region of the southern foot of the Bükk-mountains and on the creeks of the lowland Borsodi-Mezőség in 2004. 7475 specimens of 34 species were caught as the result of our surveys on 28 sampling sites. Significant differences were found between fish stocks living in wold and lowland situated sampling sites.Nevertheless on the border of the hilly and lowland regions continous transition observed in the composition of fishstocks. Using Ind Val 2.0 statistical software indicator species appointed of the stocks of wold situated samling sites in “natural” and in “disturbed” states, and of the lowland situated sampling sites.
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Analysis of sweet corn nutritional values using multivariate statistical methods
103-108Views:539Processing large amounts of data provided by automated analytical equipment requires carefulness. Most mathematical and statistical methods have strict application conditions. Most of these methods are based on eigenvalue calculations and require variables to be correlated in groups. If this condition is not met, the most popular multivariate methods cannot be used. The best procedure for such testing is the Kaiser-Meyer-Olkin test for Sampling Adequacy. Two databases were examined using the KMO test. One of them resulted from the sweet corn measured in the scone of the study, while the other from the 1979 book of János Sváb. For both databases, MSA (measures sampling adequacy) was well below the critical value, thus they are not suitable e.g. for principal component analysis. In both databases, the values of the partial correlation coefficients were much higher than Pearson’s correlation coefficients. Often the signs of partial coefficients did not match the signs of linear correlation coefficients. One of the main reasons for this is that the correlation between the variables is non-linear. Another reason is that control variables have a non-linear effect on a given variable. In such cases, classical methods should be disregarded and expert models better suited to the problem should be chosen in order to analyse the correlation system.
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The effect of genotype and the location of sampling on the mineral content of wool
157-160Views:542Mineral supplementation is very important in high producing farm animals. The estimation of exact mineral intake is very difficult in forage eating animals, like sheep. Accessing of long term mineral status seems to be possible using wool mineral analysis. However several factors can affect the results. Therefore, the aim of this study was to test the effect of breed and sampling location on the mineral content of sheep wool. 20 Dorper and 20 Tsigai sheep were chosen from the same farm. Samples were obtained from 3 locations (withers, side and quarter) and tested for 8 elements: Ca, Mg, Na, Co, Cu, P, S, Se , Zn. The samples were cleaned with ethyl alcohol from organic contamination, then after adding nitric acid were mineral analized using ultrasonic cleaning unit. The samples were analysed with ICP-OES (Perkin-Elmer, Optima 3300 DV). Statistical analyses were carried out by GLM procedure of SAS statistical analyses software. Differences between means were checked with Tukey-test. Significant breed differences were detected in the case of Mg, Na, S, Se in spite of the same feeding regime. The wool mineral content were within the reference range. The sampling location had no effect on the mineral content of wool.
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Spatially Discrete GIS Analysis of Sampling Points Based on Yield and Quality Analysis of Sugar Beet (Beta vulgaris L.)
32-37Views:258Fulfilment of the increasing quality requirements of sugar beet production can be analysed with sampling of plants and soil at the cultivated area. Analyses of the spatial characteristics of samples require exact geodetic positioning. This is applied in practice using GPS in precision agriculture. The examinations were made in a sample area located in north-western Hungary with sugar beet test plant. According to the traditional sample taking procedure N=60 samples were taken in regular 20 x 20 m grid, where besides the plant micro and macro elements, the sugar industrial quality parameters (Equations 1-2) and the agro-chemical parameters of soils were analysed. Till now, to gain values of mean, weighted mean and standard variance values, geometric analogues used in geography were adapted, which correspond to the mean centre (Equation 3), the spatially weighted mean centre (Equation 4), the standard distance (Equation 5), and the standard distance circle values. Robust spatial statistical values provide abstractions, which can be visually estimated immediately, and applied to analyse several parameters in parallel or in time series (Figure 1). This interpretation technique considers the spatial position of each point to another individually (distance and direction), and the value of the plant and soil parameters. Mapping the sample area in GIS environment, the coordinates of the spatially weighted mean centre values of the measured plant and soil parameters correlated to the mean centre values showed a northwest direction. Exceptions were the total salt and calcium-carbonate contents, and the molybdenum concentration of the soil samples (Table 1). As a new visual analysis, the spatially weighted mean centre values of the parameters as eigenvectors were projected to the mean centre values as origin. To characterize the production yield, the raw and digested sugar contents of the sample area, the absolute rotation angles of the generated vectors were determined, which indicate numerically the inhomogenity of the area (Figure 2). The generated spatial analogues are applicable to characterise visually and quantitatively the spatial positions of sampling points and the measured parameters in a quick way. However, their disadvantage is that they do not provide information on the tightness and direction of the spatial correlation similarly to the original statistical parameters.
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Complex assessment of inland water fish stocks
74-80Views:244In the domestic fish production, natural waters have yielded for several years about 7-8 thousand tons. This, from the point of view of outputs, considering the almost 130 thousand hectares of natural water, is rather low, it means approximately 55-60 kg/ha mixed fish.Although the various natural waters can differ significantly on the basis of yields, yet on the majority of the territories, the results were low. In the case of our extensive still waters and rivers, the reason can undoubtedly be found in the combined effect of the lack of the possibility of reproduction of the fish stock and the over-fishing. Fishery built on planning supposes the best possible knowledge in the given circum stances of the parameters of the water area and its fishstock. Lacking this knowledge, it is not possible to establish the optimal use fulness of the resources, what is more, the management can make faulty decisions – as a result of a lack of information -, which can risk the success of later activities.
It is known that many factors have an impact on the success of the fishery, as well as some information in connection with the water area and the fish stock are necessary, the knowledge of which make it possible to manage the fishery in a planned way. One part of the information is available, while the other part is incomplete or not deep enough. The necessary data are dissimilar depending on their nature, can be obtained from different places, by different methods.
As the first step for executing the field surveys and processing data, I developed a complex model, which contains in a unified system the steps of estimating the fishstock. I made the sampling on the basis of this. Part of the model is a fish faunistic survey, as well as a morphological survey of the water area. The information gained from these are important for making more accurate the system of devices of the samplings for stock estimation (duration, number of net-rows) and for assigning its place (places representing the best way the physical characteristics of the given water area). The major stages of stock-survey: A) faunistic survey, B) physical survey of the bed, and C) sampling with the help of gill-nets. This is followed by the evaluation by the computer module.
The results of the research create a methodological and technical background for the fish faunistic and population biologic surveys still performed in different ways in our country, and by applying these methods together, all basic information about natural waters which help decision-making concerning fisheries can be obtaine deffectively. -
The study of headspace sampling for the measurement of Volatile Organic Compounds byGC-MS
35-38Views:292Headspace sampling was studied for GC-MS measurements. A quick, direct method was developed for the measurement of petrol, gasoline and volatile organic solvent contamination of soil. According to our results this method is suitable for quick qualitative analysis of VOC contamination of soil, and helps to make decisions about further measurements. We concluded that an accurate quantitative measurement is not possible.
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Environmental Management Research on the Berettyó River
161-165Views:134Our department is involved in ongoing research into the hydrological and ecological interplay of the Berettyó River.
In the first part of our study we classified the Hungarian section of the river from ecological and hydrological standpoints. We determined three typical parts of the river: a sandy and gravely bottom, a middle part with a sandy and muddy bottom, and a lower part, with muddy bottom. In theese sampling areas we measured and established the more important static and dynamic hydrological, physical and chemical characteristics.
For the planned research we did a primary estimation of the environmental condition of theese sampling areas, on the basis of applicable biological and ecological indication methods. -
Distribution of the American grapevine leafhopper (Scaphoideus titanus ball 1932) in west Romania
127-130Views:370The vector of Grapevine Flavescens Dorée phytoplasma, the American grapevine leafhopper (Scaphoideus titanus) has been in Europe since 1924. In Romania, the first populations were detected in 2009 in the central, eastern and southern part of the country. Later, the leafhopper was found also in West Romania in 2014. In 2015 and 2017, altogether, 14 sampling sites were studied in two vine regions of this area. The Scaphoideus titanus could be detected in 10 of them with relatively small abundances. During the studies, the yellow sticky traps proved more effective methods than sweep netting. To prevent vineyards from disease, we should do everything against the vector from prevention to chemical protection.
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What does precision crop production hold for the future of soil science and plant nutrition?
411-421Views:292The concept of precision agriculture is straightforward at the scientific level but even basic goals are blurred at the level of everyday practice in the Hungarian crop production despite the fact that several elements of the new technology have already been applied. The industrial and the service sectors offer many products and services to the farmers but crop producers do not get enough support to choose between different alternatives. Agricultural higher education must deliver this support directly to the farmers and via the released young graduates. The price of agricultural land must be higher if well-organized data underpin the production potential of the fields. Accumulated database is a form of capital. It must be owned by the farmers but in a data-driven economy its sharing will generate value for both farmers and the society as a whole.
We present a methodological approach in which simple models were applied to predict yield by using only those yield data which spatially coincide with the soil data and the remaining yield data and the models were used to test different sampling and interpolation approaches commonly applied in precision agriculture. Three strategies for composite sample collection and three interpolation methods were compared. Multiple regression models were developed to predict yields. R2 values were used to select among the applied methods.
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The Role and Significance of Soil Analyses in Plant Nutrition and Environmental Protection
3-8Views:241Hungary has a rich history of soil analyses and soil mapping. Our main tasks today are the preservation of soil fertility as well as balancing the goals of production and environmental protection. The main requirement of agricultural production is to adapt to ecological and economic conditions.
In a series of consultative meetings in the past seven years, representatives from Central and Eastern Europe have analyzed nutrient management practices in their respective countries. According to a joint memorandum agreed upon in 2000, in the countries awaiting accession, the quantity of nutrients used per hectare is considerably smaller than the Western-European usage targeted through special subsidies. The current low nutrient usage contradicts the principles of sustainability and that of the efficient use of resources, jeopardizing soil fertility.
In Hungary, the use of inorganic fertilizers underwent a dynamic development, which manifested itself in an almost tenfold usage growth between 1960 and 1985. This growth slowed down somewhat between 1985 and 1990 and then reduced dramatically after 1990, reaching record lows at the usage levels of the 60s. The nutrient supply has had a negative balance for the last 15 years.
The increasing and then decreasing usage trends can equally be detected in the domestic yield averages of wheat and corn as well as in the nutrient supply of soils. Yields were the largest when usage levels were the highest, and decreased thereafter. Draughts have also contributed to smaller yields. The dramatic decrease in the use of inorganic fertilizers when adequate organic fertilizers are lacking endangers our soils’ fertility.
About 50% of soils in Hungary are acidic. Acidity is mostly determined by soil formation, but especially on soils with a low buffering capacity, this acidity may intensify due to inorganic fertilizers. Sustainable agriculture requires the chemical improvement of acidic soils. According to their y1 values, the majority of our acidic soils need to be improved. This chemical soil remediation is required in 15% of the acidic soils, while it’s recommended for another 20% of these soils.
Results of the analyses conducted in the framework of the soil-monitoring system set up in Hungary in 1992 show that in 95% of the analyzed samples, the toxic element content is below the allowable limit. Cultivated areas are not contaminated; toxicity above the legal level was found only in specific high-risk sampling areas: in the vicinity of industry, due to local overload. The basic principle of sustainable agriculture is to preserve soil fertility without undue strain on the environment. The intensity of the production needs to be considered according to the conditions of the site; i.e.; nutrient management needs to be site-specific. It is recommended to differentiate three types of cultivated land in terms of environmental sensitivity: areas with favorable conditions, endangered areas, and protected areas, and then to adopt nutrient management practices accordingly. To meet all the above-mentioned goals is impossible without systematic soil analysis. Tests conducted by the national monitoring system cannot replace regular field measurements. -
Phytoplankton diversity and their relationship with water quality parameters in the middle basin of Ogun River, Abeokuta, Southwest Nigeria
5-13Views:35Phytoplankton are crucial bioindicators for assessing freshwater ecosystem health. This study investigates the diversity and distribution of phytoplankton and key water quality parameters in the Ogun River. Monthly samples were collected from three sites along the river between February and August 2024. Phytoplankton samples were preserved with 4% formalin and analyzed microscopically, while water quality parameters, including temperature, pH, dissolved oxygen, conductivity, transparency, total dissolved solids, nitrate, phosphate, alkalinity, and hardness, were measured using standard methods. Results showed temperature ranged from 27.3–31.9 °C, pH from 6.7–9.4, dissolved oxygen from 4.3–7.2 mgL-1, conductivity from 10.8–20.9 µS/cm, transparency from 0.27–1 m, and other parameters within specified ranges. Sixteen phytoplankton species from 10 families were identified, with the Bacillariophyceae family being the most dominant, representing 7 species. Lyngbya spp. was the most abundant species, followed by Pediastrum simplex. Species richness was 12 species at Site A, 13 at Site B, and 14 at Site C. Dominance values were 0.13, 0.11, and 0.12, respectively. Simpson’s diversity index ranged from 0.87 to 0.89, and the Shannon-Weiner index was 2.24 at Site A, 2.38 at Site B, and 2.35 at Site C. One-way ANOVA tests showed no statistically significant differences in diversity indices among the three sampling sites (p > 0.05), indicating relatively consistent phytoplankton diversity across the study area. These findings highlight the importance of integrated biological and physicochemical monitoring for effective water management and ecosystem conservation in the Ogun River.
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Anthropogenic effects on the fish fauna of River Hernád
21-27Views:269The fish fauna of the River Hernád was examined in 24 sampling sites in summer of 2014. As a result of the survey 31 species were detected of which eight are protected and three are highly protected by law in Hungary. The presence of the adventive tubenose goby (Proterorhinus semilunaris) in the estuary of the Hernád was observed for the first time by us. The most frequently detected species (with 100% frequency of occurrence, FO) were the chub (Squaliuscephalus), the bitterling (Rhodeusamarus) and the bleak (Alburnusalburnus), in addition the bleak was also the most dominant fish of the river. The hydraulic works have detrimental effect on the number of species and specimens and on the diversity of the fish fauna as well. The natural sections of the River Hernád have the most diverse fish assemblages.
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Element Content of Herbaceous Plants in the Floodplain Meadows
55-58Views:371Animals require well-balanced nutrition. The elemental content of the vegetation of meadows is influenced by as many factors such as heat, rainfall, irrigation, soil type and nutrients, meadow types, species, aspects of the vegetation period and cultivation.
Natural meadows used extensively are common sights on river floodplains. Since chemicals are banned and the species number is high, measuring the elemental composition of plants on these meadows is beneficial. Cenological survey and element content measurements were held on the rich flora of four natural meadows in the year 2001.
Weeds, in a wider sense, are plants not directly involved in growing, although their nutritional values make them important costituents of feed. Meadows are enriched by their relatively high microelement content.
On the sampling sites, the ratio deviated from the ideal 2/3 parts monocotyledon and 1/3 part dicotyledon, but this did not mean a Mn deficiency as it would have been assumed. -
Composition and role of hoverfly (Diptera: Syrphidae) communities in an organic apple orchard
57-61Views:177The use of hoverfly (Syrphidae) larvae in biological control is gaining more attention where the damage by their prey, aphids is significant, especially in agricultural fields and orchards, where they lower the number of aphids. The present study focuses on one hand on faunistics, on the other hand on testing the different available collecting methods for later research. Collecting in an organic apple orchard near Debrecen yielded 525 specimens of Syrphidae mostly by netting but also with white tray traps. Collected species are differentiated according to their feeding (94.1% aphidophagous). The three species found in greatest numbers are Sphaerophoria scripta (Linnaeus, 1758), Melanostoma mellinum (Linnaeus, 1758) and Episyrphus balteatus (De Geer, 1776). The available information on their biology is reviewed and their potential value of hoverflies in biological control is discussed.
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Folic acid content of beetroot leaf and root by different growing stages and genotypes
115-119Views:438An increasing interest has been observed of beetroot leaf as a salad component due to recent studies focusing on their nutritional value. The randomized field experiment was carried out on lowland chernozem soil with 6 varieties, 3 replications and 2 sowing dates. Sampling was performed on 23 of August 2018 at the stage of 30 and 50 days of vegetation, where leaf (30 and 50 days) and root (50 days) were collected. Total dry matter, folic acid and nitrate content were evaluated.
The results of this investigation show that higher total dry matter content was measured in the root (8.47–10.30%) compared to the leaf in both developmental stages (6.47–9.20%). Nevertheless, higher folic acid content was found in the young leaves of 30 and 50 days of development (58.77–113.86 µg 100g-1). Among the examined varieties, Bonel has presented great amount of folic acid not only in the leaves (99.35–113.61 µg 100g-1), but also in the root (89.99 µg 100g-1). Finally, lower nitrate content was found in Libero (316.16 mg kg-1) at 30 days and in Akela (340.41 mg kg-1) at 50 days of development. Thereby, fresh consumption of beetroot leaves are highly recommended.
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Evaluation of dry matter accumulation of maize (Zea mays L.) hybrids
35-41Views:881The increase of the grain yield of maize is closely correlated with its seasonal dry matter accumulation. Dry matter is accumulated into the grain yield during the grain filling period. The following maize hybrids were involved in the experiment: Armagnac FAO 490, Loupiac FAO 380 and Sushi FAO 340. In order to determine dry matter content, two samples per week were taken on the following days: 22nd, 25th, 28th, 31st August, 4th, 7th, 14th, 18th, 22nd, 25th, 29th September and 2nd, 6th, 9th, 13th October. In the course of sampling the weight of 100 grains from the middle section of 4 ears was measured in 4 replications. Dry matter content was determined after drying to constant weight in a drying cabinet at 60 °C. Harvesting was performed on 13th October 2017.
The daily precipitation sum was determined by local measurements, while the daily radiation and temperature data were provided by the Meteorological Observatory Debrecen of the National Meteorological Service in Budapest. Among the agrometeorological parameters, an analysis was made of the precipitation during the growing season, effective heat sums during the vegetative and generative phase, and the water supplies. The daily heat sums were determined using the algorithm proposed.
The amount of precipitation in the winter period before the 2017 growing season was 210 mm. The soil was saturated until its field capacity. The rather dry and warm March and April had a favourable effect, but there was no worthy amount of precipitation until May (51 mm) due to the condition of the dried seedbed. Sowing was performed on the 5th of May 2017 in a randomised small plot experiment. There was favourable precipitation and temperature during the growing season, thereby providing ideal conditions for maize development, growth and yield formation. There was near average amount of precipitation in each year. The total amount of precipitation in the summer period is 342 mm. Temperature was mostly above the average, but there was no long and extremely warm period.
The Armagnac hybrid reached its highest dry matter mass 126 days after emergence. Physiological maturity was reached sooner (on the 119th day) in the case of Loupiac, and even sooner in the case of Sushi (116th day). The thousand grain weight of Sushi (which has the shortest ripening period) was 286 g at the time of physiological maturity, while that of Loupiac was 311 g. Compared to Sushi, Armagnac showed 12 g more dry matter accumulation (306 g). In the case of all three examined hybrids, physiological maturity was preceded by an intensive phase, when the dynamics of dry matter accumulation was rather quick. On average, Sushi gained 2.8 g dry matter per day between 103 days following emergence and physiological maturity, while the same values were 3.2 g for Armagnac and 3.3 g for Loupiac. The aim of the regression line slope is to predict the behavior of the dependent variable with the knowledge of the values and characteristics of the independent variables using the regression line equation. Furthermore, to determine how the location affected the dynamic of dry matter accumulation in the Armagnac, Loupiac and Sushi hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97%, in the Loupiac 94%, in the Sushi 90 %. The determination coefficient (R2) is useful in determing how the regression equation fits. But, as we have seen, the determination coefficient alone is not sufficient to verify the model’s accuracy, in addition to the determination coefficient (R2), the normality of the data or the residuals, the variance of the variables at different levels, the independence of the data relative to time and non-oblique. Observations are evaluated for the correctness of the fitted model.
Dry matter values decreased evenly and slightly following physiological maturity. According to our research results, it was established that physiological maturity is followed by a moderate dry matter loss. Until harvesting, Armagnac lost 40 g of its thousand mass weight in 29 days, while the same value pairs were 69 g in 36 days for Loupiac and 29 g in 39 days for Sushi. Loupiac – which had the highest weight at the time of physiological maturity – lost the most of its dry weight; therefore, Armagnac and Sushi had higher values at the time of harvesting.
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Examination of compost maturity using reflectance
29-34Views:497Composting is one of the most popular recycling processes for organic waste. Composting plays an important role in waste and by-product management and is becoming increasingly important in both sustainable energy management and circular economy. Composting transforms organic matter to produce a safe and stable by-product (compost) that can be applied to arable land in a similar way to fertilizer. Physical, chemical and biological methods can be used to monitor the process and to determine the maturity of the compost, as spectrometric/spectroscopic methods play an important role in the analysis of different environmental samples.
Our aim was to (1) non-destructively detect the effects of different additive ratios on the spectral properties of the composting process and the spectral data of different compost mixtures, (2) to find the wavelength ranges of the reflectance curve (inflection points) sensitive to compost maturity, (3) to determine the correlation between the inflection points and the chemical and physical parameters measured in compost by conventional methods.
The mixture of broiler and hen manure and zeolite was composted 62 days in windrow composting. In the composting experiment, the moisture content and temperature (°C) were measured every three days and compost samples were taken and in 10% destillated aquaeous suspension were measured the pH and electrical conductivity (mS cm-1). Compost samples dried to mass stability were spectrally analyzed in the wavelength range 400–1000 nm with AvaSpec 2048 spectrometer.
Based on the results, the reflectance of mature compost were smaller in the last days of composting than the reflectance values of day 0 samples, thus compost maturity can be detected spectral in the VIS-NIR wavelength range. For the tested compost prisms, the reflectance of each sampling day shows a constant slope, with a significant overlap of the reflectance curves up to 400–700 nm wavelength range, and there was a breakpoint in the 700–750 nm wavelength range which was proved by binary encoding.
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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
81-85Views:287The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.
Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.
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Effect of Soil Covering on the Soil Enzyme Activity of Integrated Orchard
20-29Views:174The purpose of our experiments is to discover the effect of different soil cover matters (agrofoil and black polyethylene) on the activity of some enzymes (phosphatase, saccharase, urease, catalase, dehydrogenase) occuring in soil. Soil samples were taken from a cider apple plantation of the Fruit Producing Research and Advisory Kht Újfehértó. The enzyme activity was measured according to Krámer and Erdei (1959a), Kuprevič and Tsherbakova (1956), Kuprevič et al. (1966), Frankenberger and Johanson (1983), Mersi and Schinner (1991). Soil moisture content was by conventional (drying chamber) method measured during every sampling and enzyme activity was transpolated to absolute dry soil. Results were estimated by mathematical methods (variation analysis, correlation counting). Soil samples were taken by trials 5 times (in every two months) a year in the vegetation period from March to November.
By recording the monthly changes of the enzyme activity we have observed the following. The activity of the phosphatase was generally the highest in May and the lowest in November. Depending on the trials, high values were also measured in March and September. The activity of the saccharase was generally the highest in November and the lowest in June, but at the same time peaks even occured in May and September. The highest urease avtivity was measured in September and November, and the lowest activity in May and July, also depending on the trials. In the year 2000, after a deep point in March, the activity of the catalase was the highest in November or by certain trials in September. In 2001, the lowest activity was also measured in March, but the highest activity appeared in November in case of one-minute trial, and in May in consequence of two-minute trial. Finally the activity of dehydrogenase was the highest in November and the lowest in July apart from the model years.
There were essential differences in rainfall of the two experimental years which was reflected in the enzyme activities. There was a poor positive significant relationship between soil moisture content and enzyme activity values in case of phosphatase, saccharase, urease (r=0,426; 0,480; 0,396) respectively. In case of catalase1 (r=0,518), catalase (r=0,556), dehydrogenase (r=0,559) we obtained a medium strong positive relationship between soil moisture content and enzyme activity values. By evaluating the effect of different trials in case of every examined enzyme significantly higher values were detected in soils covered by agrofoil (a porous black polyethylene) than in soils covered by black polyethylene or in uncovered soils. Moreover, the soil covered by black polyethylene showed significantly higher enzyme activities (besides phosphatase) than the control soil. Thus soil-covering meant statistically significant advantages in enzyme activity as opposed to uncovered soil proved. -
The variation of breast milk composition during the lactation stage for mothers from the Csík region
93-98Views:245The aim of our research was the examination of breast-milk composition from mothers living in the Csík region and to follow their milk composition variations during lactation. In this article we present the results obtained from three mothers, paying particular attention to essential components. The breast milk samples were collected from healthy mothers with similar habits and age. The milk samples were collected with a hand pump at the
same time after the feeding. The sampling period was from day 5 to the 14th weeks of lactation. The nutrition of mothers was recorded on a questionnaire, completed by the mothers themselves. Comparing our experimental results with data in the literature it was concluded that the milk protein content was very similar to the milk of mothers from other European countries, and is decreased during lactation.
The total saturated fatty acid content was lower, but the palmitic acid content was slightly higher. Regarding the essential fatty acid composition the linoleic- and the arachidonic acid contents were appropriate from a nutritional point of view. The linolenic acid was present in lower amounts, but the docosahexanoic acid was almost undetectable.
The iron and manganese contents were in good agreemen with published results, but the zinc content of the breast milk was lower and its copper content was higher. These differences in milk composition can be explained by the different nutritional habits of our subjects. -
Data on the bumblebee assemblages (Apidae: Bombus spp.) lives in lands under agri-environment commitment
31-35Views:415The goal of agri-environmental schemes (AES) and greening programs are protecting and increasing biodiversity in agricultural lands. The evaluation of effectiveness of AES needs further investigations. For the purpose of investigations, species and species groups should be selected which can indicate the effects of changes in landscape use on biodiversity. Bumblebees are good indicators for this purpose.
The role of bumblebees in pollination is well studied but in the case of different crops, much less detailed data are available. In 2018, bumblebee assemblages of 44 sites belonged to 8 different agricultural and semi-natural habitat types were studied in the surroundings of Sajószöged, Tiszaújváros and Derecske.
This study provides new distribution data of 8 bumblebee species in three 10×10 km UTM cells covering the sampling area. According to our results, the alfalfa and red clover fields and semi-natural grasslands has more species rich and abundant bumblebee assemblages than different crop fields (sunflower, oilseed radish and vegetable morrow) and can help protect bumblebee assemblages of agricultural lands. Based on the collected distribution and abundance data, the role of the bumblebees in pollination of the studied crops should be re-evaluated.
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The effect of wool staple length on the mineral content using the same sheep feeding regime
97-100Views:526The wool of sheep is suitable to test the mineral supply; however, a number of factors could affect the results. The growth rate and length of staple can be very different according to season, physiological state and individual variation. These factors are likely to affect the quantity of minerals accumulated into the wool. Therefore, the purpose of this research was to examine whether there is a difference between the mineral content of wool nearly reached the full staple length and the freshly grown wool using the same feeding regime.
10 Tsigai pregnant sheep have been selected randomly from the same farm. Wool samples were obtained from the withers, side and quarter with bended scissors. Samples were mineralized using nitric acid and hydrogen peroxide using ultrasonic cleaning unit. P, Ca, Mg, Na, S, Cu,Se and Zn content were determined by ICP-OES. Statistical analyses were carried out by SAS (SAS Institute Inc., Cary, NC) GLM procedure.Differences between means were tested by Tukey test. Significantly lower Ca, Na, P, Zn values were found in case of intensively grown wool. Sampling location did not affect the mineral content. Herd mineral supply was adequate. Our results suggest that intensively grown wool samples have to be used for mineral analyses. -
Chromatometric comparison of Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) feathers
19-22Views:478Chromatometric examination of the plumage of birds is a poorly researched topic. We have approached this issue in primarily aspect of differences in plumage of species. Moulted feathers sample collection method has been increasingly used. Reliable identification of feathers becomes an increasingly important issue, hence need for an exact measurement-based methodology. Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) primary, secondary and tail feathers were studied. Chromatometric parameters of feathers were measured in CIELAB color system and then statistical analysis (Independent samples t-test, Descriptive Statistics, Discriminant Analysis) was performed to compare the two species. Instrumental measurements has been confirmed the high similarity between colors of the two species, however species specific differences were also found. Lightless (L*) value were significantly characteristic of particular species, while the red/green (a*) and yellow/blue (b*) value had lower Predictive Power. We identified feathers and the variables which useable to separate the two species and determined the associated Confidence Intervals of these values. Our results may draw attention to a new potential direction for exact identification of the moulted feathers during sample collection.