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Effect of Ozone Exposure on Phytopathogenic Microorganisms on Stored Apples
Published December 14, 2004

The aim of our study was to clarify the effect of ozone exposure on several phytopathogenic fungi on stored apple fruits under different storage conditions. The study was conducted at Bistrita, Romania, in the storehouse of an experimental apple orchard in 2002 and 2003. Two widely grown apple cultivars (‘Jonathan’ and ‘Golden Delicious...) were used. General microbial examination of the fruits was made during storage in order to identify the most important storage pathogens. Efficacy of six ozone treatments was evaluted on fruit decay caused by phytopathogenic fungi. Monthly observations (January, February, March and April) were made of the degree of decay and three measurements were assessed (disease frequency, disease intensity and degree of attack). Our results showed that the most important phytopathogenic fungi during storage was blue mold, caused by species of Penicillium. Disease frequency of apple fruits was very high on cv. ‘Jonathan’, much higher than on cv. ‘Golden delicious’. Ozone treatments (25 ppm ozone for 0.5 and 1.5 hours in November) caused significantly lower disease incidence on stored apple than all other ozone treatments. For longer storage, it seems that additional ozone treatments in February increased treatment efficacy. Cv. ‘Golden delicious’ seemed to be more resistant to storage diseases than cv. ‘Jonathan’ both on the untreated and treated fruits. The effect of the ozone treatments was also the most effective when 25 ppm ozone was applied for 0.5 and 1.5 hours in November.

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The examination of flesh firmness in different apple varieties
Published December 15, 2019

Of the different physical features of fruits, one important quality feature is flesh hardness. This is an important parameter to be considered when selling fresh apples, for the manufacturing industry and when entering into storage. In our research, we examined 7 apple varieties, in 2016 and 2017, from the establishment of KITE Zrt. in Derecske... and the Horticultural Experimentation Site of Pallag (Pallagi Kertészeti Kísérleti Telep). The plantations took place in accordance with intensive apple production. In both years, samples were collected at the time ripening of the given apple variety. Of the examined apple varieties, 5 of them are resistant to scab (Gaia, Isaaq, Modí, Smeralda, Fujion), 1 is not resistant (Golden Reinders) and 1 (Pinova) is moderately susceptible to scab. The scab resistant apple varieties were correlated to the non-resistant Golden Reinders. Flesh firmness was measured with a fruit penetrometer in each of the seven apple varieties. In the first year a two-month storage experiment was carried out, where the flash firmness after harvest, and after one-month and two-month storage was measured. The results were assessed with the help of the IBM SPSS Statistics 20 program. The goal is to compare the apple varieties based on their flesh firmness, in particular with regard to the resistant varieties.

The Isaaq 8.81–8.82 N/cm2 , the Modí 9.18–9.59 N/cm2 and the Fujion 8.66–9.13 N/cm2 , all of which are scab resistant apple varieties, showed a significant initial flesh firmness. During storage, the Pinova apple variety flesh firmness changed the most favourably.

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Loss and Disease Development of Monilinia fructigena (Aderh. & Ruhl.) Honey in an Organic Apple Orchard
Published December 14, 2004

In a two-year-study, yield loss and temporal development of incidence of Monilinia fructigena were quantified in organic apple orchards and the importance of fruit wounding agents was determined. The first infected fruits were observed at the beginning of August in 2001 and 2002. Disease development was continuous until fruit harvest in both ye...ars. Pre-harvest yield loss caused by Monilinia fructigena amounted on average 27.2% in 2001 and 41.6% in 2002 by fruit harvest. The growth rate of disease development was almost double in 2002 compared to 2001. All infected fruits were injured by wounding agents such as aboitic and mechanical injury factors, codling moth (Cydia pomonella), common earwig (Forficula auricularia) and birds. In this study, the most important wounding agents were codling moth and mechanical injury factors in organic apple orchards. In both years, our results showed that 70-80% of the infected fruits were damaged by codling moth in organic apple production. Moreover, 10-15% of the infected fruits were mechanically injured in the two years. Our results indicated that most of the damaged fruits fell on the orchard floor before harvest and they became an important secondary inoculum source of M. fructigena. Biological and practical implications of the results are discussed.

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Flesh firmness examination of scab-resistant apple varieties in a storage experiment
Published December 1, 2020

Of the different physical characteristics of fruits, one of the most essential qualities is flesh firmness, as is an important parameter when selling fresh apples, processing the fruit (processing industry) and during its storage. In the scope of our research, we examined five apple varieties originating from the Derecske horticultural site... of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The five examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The two-month storage experiment was conducted at 16-17 °C, which had an aggressive effect on our stored apples. Flesh firmness, weight and diameter were measured on three dates for each of the five apple varieties. Our results were evaluated using IBM SPSS Statistics 20 software. We aimed to compare flesh firmness, weight and diameter of the five apple varieties and their changes during storage. Isaaq (86.34 N/cm2), Modí (94.06 N/cm2) and Fujion (84.90 N/cm2) had outstanding initial flesh firmness results. The Modí apple variety provided the best storage parameters.

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The Effect of Sunburn on Fruit Quality of cv. Idared Apple
Published March 4, 2006

The aim of this study is to investigate the effect of sunburn injury on fruit quality parameters (colour-coverage, depth of affected tissue, fruit flesh firmness, soluble solids content) of apple.
The symptoms of sunburn injury appeared in concentric ring shape, differed from each other and surface colour-coverage. This can be due to the of the injury. The authors observed the following colours on the fruit surface (from the epicentre of the blotch on the transversal diameter of the fruit) dark brown (strongly affected), light brown (moderately affected), pale red transition (poorly affected), red surface colour-coverage (not affected).
Sunburn of apple fruits is a surface injury caused by solar radiation, heat and low air relative humidity that in the initial phase results in a light corky layer, golden or bronze discolouration, and injuries to the epidermal tissue, in the surface exposed to radiation. Thus it detracts from its appearance, but in most cases, it would not cause serious damage to the epidermal tissue. The depth of affected tissue is not considerable, its values are between 1.5-2.0 mm generally. It is commonly known that the tissue structure of apple fruit is not homogeneous. Accordingly, the degree of injury shows some differences under the different parts of the fruit surface.
On the basis of flesh firmness research, the authors established that the measure of flesh firmness of the affected part of apple fruit increases with the effect of sunburn. The consequence of this is the suffered plant cells will die, the water content of this tissue decreases and the fruit gets harder. This water-loss caused the increase of soluble solids content.

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Effect of M9, MM106 and Seedling Rootstocks on Sunburn-Sensitivity of 33 Apple Cultivars, and Sunburn Effects on Fruit Quality
Published December 6, 2005

The purpose of this study was to investigate the sunburn-sensitivity of 33 apple cultivars grafted onto 3 rootstocks (M9, MM106 and seedling) in a commercial orchard at Nagykutas (in the western part of Hungary). The authors also searched for any relationship between fruit quality parameters and the frequency of sunburn on the fruit surface....>During the observations, the cultivars had rootstock-specific properties in respect to sunburn-susceptibility. Accordingly, the injury decreased in the order M9, MM106 and seedling rootstocks. The differences in the sensitivity were founded on the foliage-morphological characteristics of trees, caused by the growing vigour of the rootstocks. Accordingly, the highest value of sunburn injury was observed on M9 rootstock, because this rootstock has a dwarfing effect on the grafted main cultivars. Thus, the vegetative area of these trees grew very slowly and the foliage was not compact enough to protect the fruits from the strong rays. The largeness and density of the foliage increased in the order M9, MM106 and seedling rootstocks. Relationships were also demonstrated between the diameter of the upper part of crown, the size of leaves, the number of fruits per tree and the injury from sunburn.
The damage values showed, that the gravity of symptoms did not decrease below a well-defined level of dimension on fruit. Determined potential area of injury was necessary for the symptoms to become visible.
The authors categorized the cultivars in the respect of values of sunburn frequency: I. „Not sensitive”, II. „Moderately sensitive” and III. „Very sensitive” categories were constituted. Generally, the Gala cultivars showed low damage (or were free of symptoms), in contrast, Golden mutants suffered relative strongly. The most sensitive cultivar was Jonica on all three of rootstocks.
We searched for any relationship between the fruit quality parameters and the frequency of sunburn. A significant correlation was found in the cases of fruit weight and colour-coverage. The latter can be related to that fact that highly colour covered fruits are found on the peripherial part of crown, so these are exposed to stronger sun rays

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The effect of daytime and nighttime temperature on the cover colour of fruits in an apple gene bank
Published September 14, 2005

Skin colour of fruits is an important fruit quality parameter. Fruit growers know the phenomenon that the apple colouration is very good in one year while in other years the green and red apples can be differentiated only on the basis of the morphological characteristics of the fruits. There are great differences in values of cover colour betwe...en years.
In the first step, the relationships between day and night temperature, the difference between day and night temperature and fruit skin colour should be determined. In this study, the authors investigate and quantify this relationship.

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The effect of sunburn damage on the fruit quality parameters of apple (Malus domestica cv. Idared)
Published September 14, 2005

In the present study the authors investigated the effect of sunburn injury on fruit quality parameters (cover colour, depth of tissue damage, fruit flesh firmness, dry matter content) of apple.
The symptoms of sunburn injury appeared as concentric rings, differing in colour from each other and the cover colour. This can be connected with the... ratio of the injury. The authors observed the following colours on the fruit surface (from the epicentre of spots on the surface of the fruit) dark brown (strongly damaged), light brown (moderately damaged), pale red transition (weakly damaged), red surface cover colour (not damaged).
Sunburn of apple fruits is a surface injury caused by solar radiation, heat and low relative humidity. In the initial phase, a light corky layer, golden or bronze discolouration and injuries of the epidermal tissue appear on the surface exposed to radiation. Thus, it detracts from the fruit’s appearance, but in most of the cases it would not cause serious damages in the epidermal tissue. The depth of tissue damage is not considerable, its values are between 1.5-2.0 mm in general. It is commonly known, that tissue structure of the apple fruit is not homogeneous. Accordingly, the degree of injury shows some differences under the different parts of the fruit surface.
On the basis of the flesh firmness studies, it can be stated that the flesh firmness of the damaged parts increases due to the sunburn effect. This is due to the fact that the damaged plant cells die, the water content of the tissue decreases and it hardens. However, due to this reduction in the water content the dry matter content will increase.

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Crop Load, Fruit Thinning and their Effects on Fruit Quality of Apple (Malus domestica Borkh.)
Published October 11, 2006

Crop load, a quantitative parameter used by industry, is generally defined as the number of fruit per tree. It is often expressed in terms of number of fruit per trunk cross-sectional area (fruit/TCSA). Crop load is the most important of all factors that influence fruit size, and the removing of a part of the crop is the most effective way to i...mprove fruit size.
The potential size of a given pome fruit is determined early in the season and growth proceeds at a relatively uniform rate thereafter. This uniform growth rate permits the accurate prediction of the harvest size of the fruit as early as mid-summer. The growth rate, once established, is not easily altered, and fruit numbers, therefore, can affect fruit size only within definite limits and maximum effectiveness requires adjustment in fruit numbers relatively early in the season. It was established, that „thinning does not change a potentially small fruit into a large fruit, but rather insures that a potentially large fruit will size properly.” Emphasis should be on estimating fruit numbers rather than fruit size.
Fruit thinning can quickly reach the point of diminishing returns. Rather than a high percentage of large fruits, the objectives of thinning should be the elimination of the smallest fruits, improved fruit quality and annual production. Fruit thinning is accomplished by hand or chemical thinning. Chemical thinners are separated into categories as bloom thinners and post-bloom thinners. Early removal of potential fruit (blossom thinning) is currently used in many apple producing areas to enhance flower initiation for next year’s crop and thus, return bloom. It also results in reduced competition for photosynthates. Blossom thinners usually have a caustic effect on floral parts.
The amount of fruit left on a tree should be determined by the vigor and general condition of the tree. Leaf area per fruit affects the number of spurs flowering the following season. It can be difficult to separate timing and fruit number effects in crop loading studies, as abscission rates after hand thinning of retained flowers/fruitlets tend to very with the time of hand thinning.

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Phytoplasma diseases on fruits in Hungary
Published November 2, 2014

...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">In the last twenty years, three phytoplasma diseases were identified in Hungary, viz. European Stone Fruit Yellows (ESFY) (caused by Candidatus Phytoplasma prunorum), pear decline (caused by Candidatus Phytoplasma pyri), and apple proliferation (caused by Candidatus Phytoplasma mali). Candidatus Phytoplasma prunorum was isolated from apricot, peach, plum and japanese plum. Cacopsylla pruni the vector of ESFY was also isolated and identified. Infection of Candidatus Phytoplasma pyri was diagnosed from pear and Candidatus Phytoplasma mali was found on apple and pear. The three phytoplasmas cause different damages on their host plants. The most economically important phytoplasma disease is the ESFY. It seriously impairs apricot and japanase plum trees. After infection of apricots and japanese plums show yellowing and defoliation, and within a few years die in apoplexy-like symptoms. The disease on japanese plum is so severe that this fruit practically can not be cultivated in Hungary. Pear decline is the most serious problem especially in intensive pear plantations. The vector Cacopsylla pyri, C. pyrisuga and C. pyricola can be found in almost all pear orchards. Because of the regular presence of psyllids in intensive pear orchards the insecticide control is necessary. Apple proliferation is not an important disease in Hungary. All of our isolations of ’Candidatus Phytoplasma mali’ occured in organic orchards and record was not available in Hungary lately.

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N-Phenyl-Phthalamic Acid and Fertilization Effects on Flowering, Fruit Set and Fruit Quality of Apple (Malus domestica Borkh.)
Published October 11, 2006

On seven apple cultivars (Gala Must, Gloster, Granny Smith, Idared, Jonagold, Jonathan Csány 1, Mutsu), the authors studied the influence of N-phenyl-phthalamic (PPA) acid and fertilization on flowering, fruit set and fruit quality in the years 2003-2004. The research results showed that PPA application extended the flowering time of the most ...cultivars. Fruit set of apple cultivars increased in many cases when regulator was applied. The additional nutrient supply could increase the fruit set too. The increase of fruit set increased the number of fruit per tree. Quality parameters determined by fertilization and fruit number per tree. For example, when N-phenyl-phthalamic acid was applied without fertilization the fruit number per tree increased considerably however, it caused a considerable frittering away. The reason for this is that fruit set was high and fruit received not enough nutrition for growing up. The skin colour of fruits was decreased in several cases by the treatments. In one respect the enlarged fruit number was shaded the others and the fertilization increased the vegetative shading leaf area.

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Sour cherry anthracnose and possibilities of the control with special regard to resident Glomerella population in sour cherry plantations of East Hungary
Published November 10, 2010

Anthracnose is considered one of the most destructive diseases for sour cherry production due to the rapid development of the disease on fruits. Glomerella cingulata (Stoneman) Spauld. & H. Schrenk (anam.: Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz.) has been the fungal pathogen responsible for anthracnose in last deca...des. Yield losses greater than 90% may occur under epidemic conditions. C. acutatum (J.H. Simmonds, 1968) strains were isolated of sourcherry plantations in East Hungary and this pathogen, new for Hungarian microbiont became recently dominant. Contrarily to the former species it is certainly transmitted with ants during fruit ripening. About third of strains proved to be cutinase producers that enable them to actively penetrate via cuticule, and these strains infect directly berries of blackberry, grape and tomato as well as plum and apple. Most of cutinase negative strains could also infect these fruits after mechanic injury. All strains of both species produce amylase, cellulase, lecithinase, lipase, polyfenoloxydase and protease in vitro, although the activity of these enzymes highly varied in the medium. The only C. acutatum strains produced noticeable amount of chitinase. Strains, tolerant to recently applied fungicides to control the anthracnose, could be isolated of sour cherry plantations that might be the cause of ineffectiveness of control measures in 2010. The mycofungicide containing mixture of three Trichoderma species in oil carrier could efficiently depress the development of anthracnose in ripening sour cherry.

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Experiment of quality properties of dehydrated fruits
Published October 10, 2008

The lyophilization is the joint application of freezing and drying. It is an up-to-date conserving procedure, the point of which is that the humidity existing in the frozen humid material is transferred from the solid state directly into the gaseous state at a temperature below 0 oC under vacuum. Out of the procedures applied nowadays, this is ...the most tolerant drying process.
With regard to the high investment and operational costs, freeze drying is applied only for valuable, heat-sensitive materials when the technological aim is to preserve such properties as aroma, taste and colour as well as such components as proteins and vitamins. This procedure is suitable for drying and conserving certain foodstuffs, stimulants, organic chemicals, medicines and similar sensitive and valuable materials.
In our institute, we have been conducting freeze drying experiments with regional fruits and vegetables since the year 2005. During the first phase, we examined the heat- and material transfer as well as the abstraction of humidity, while during the second phase we analysed the rehydration ability and nutrient content of the freeze-dried materials as compared to those dried with the method of convection. Moreover we have conducted penetration measurements with a portable hardness tester.
To sum up the results gained so far, we can state that the quality of the lyophilized materials is better than those dried in the traditional way. It originates partly in the fact that the temperature and pressure applied for the freeze drying are smaller and the drying period is far longer than for the convection drying.
In contrast to convection-dried materials, freeze dried materials set in close to their original water-content, keep their original shape and size after being rehydrated. The reason of it the porous, spongy structure (flexible cell wall) of the lyophilized products which is able to take up moisture quickly. In addition, the lyophilized products can be rehydrated faster than those dried in the traditional way.
Regarding the results of the chemical analyses, the following conclusion can be drawn: the vacuum freeze drying results a small decrease of nutrient content and nutritive value for the lyophilized products.
The results of the hardness tests support the statement that the majority of agricultural materials cannot be considered as an ideal flexible body, because during the experiment the flexibility coefficient changed when going from the surface of the material inwards. In addition, the penetration tests also confirm that the surface of the convection-dried vegetables is at least 1.5-3 times harder than that of the freeze-dried products. The reason of it that it takes place during the drying denaturation processes.
The article summarizes the results of our research work listed above, in accordance with our experiments conducted by using the characteristic fruits (apple, plum) of the Nyírség Region.

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Contexts between apple orchards with various cultivar comparisons and the effect of ATS (ammoniumthiosulphate) on fruit thinning
Published March 20, 2013

...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">Our research focuses on a fruit thinning material that can also be used in apple production. This material reduces significantly the required manual labor of hand thinning by russeting the blossoms selectively. The ATS (ammonium-thiosulphate) acts as chemical desiccant contrary to the nowadays commonly used materials such as naphthalene acetic acid, naphthyl acetamide, benziladenin and ethylene, which affect the metabolic processes of the plant by regulating the hormone system. In our experimentals cultivar ’Pinova’ and ’Golden Reinders’ were treated with different concentrations of ATS. The effect of these doses on the fruit setting and the quality and quantity parameters of the fruits was studied. According to our results, in the case of cultivar ’Pinova’ the ATS did not have any detected effects at the concentration of 1.5%. Application of ATS at 3% decreased considerably the fruit setting and fruit yield, accordingly the mean fruit size improved. The response to treatment in the case cultivar ‘Golden Reinders’ does not have any similar consistent results.

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