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  • Micropropagation of Leuce-poplar clones and its role in selection breeding
    43-48
    Views:
    381
    Leuce-poplars (mostly white poplar and its natural hybrid grey poplar) are native tree species trough Hungary. They are covering more than 4.0 per cent of the Hungarian forested area (77 000 ha). The white (grey) poplars play a significant role in the forestation under sandy soil site conditions as well as they are of importance from nature conservation point of view as well.
    Long-term selection breeding work is going on at the National Agricultural Research and Innovation Centre Forest Research Institute (NARIC FRI), involving selection of fast-growing Leuce-poplar clones under dry site conditions.
    Micropropagation technology is relatively quickly spreading in forestry. In vitro multiplication of trees is applied mainly in fruit growing in Hungary, in case of forestry it is used mainly for selection breeding.
    This paper presents a short overview on the micropropagation trials with different Leuce-poplar clones and the early evaluation of the seedlings growing of the micropropagated clones/varieties.
  • Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
    100-104
    Views:
    336

    Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitis
    species were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types of
    molecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

  • Examination of the Effect of Cropyear on the Yield Potential and Yield Stability of Winter Wheat Varieties
    62-67
    Views:
    428

    Variety selection is one of the most important, determinative elements of sustainable winter wheat production. Yield potential, and yield stability are the most important elements in the variety selection of winter wheat, but baking quality parameters play an important role, too.
    Several winter wheat varieties were tested for yield and yield stability on chernozem soil in the Hajdúság (in the eastern part of Hungary), in the 2001-2002-2003-2004 cropyears. The management factors were the same for all cropyears. 15 varieties in early the maturity group, 14 varieties in the middle maturity group and 4 varieties in the late maturity group were tested in the above mentioned cropyears. The climatic conditions were average in 2001, dry in 2002, extremely dry in 2003, and very favourable in 2004.
    We obtained 5298-6183 kgha-1 yield from early maturity varieties, 5683-6495 kgha-1 from middle, 5694-6031 kgha-1 from late ones in the average of four years. The cropyears had strong influence on the yields, even on chernozem soil, and were characterized by excellent water – and nutrient – husbandry. Averaging of cropyears and genotypes, we obtained 6984 kgha-1 in 2001 (average cropyear), 5452 kgha-1 in 2002 (dry cropyear), 3120 kgha-1 in 2003 (extremely dry cropyear) and 8400 kgha-1 in 2004 (optimum cropyear), respectively. The yield differences between the minimum and maximum yields were 885 kgha-1 in early varieties, 812 kgha-1 in middle and 337 kgha-1 in late maturity varieties, respectively. The varieties characterized by high yield potential and the varieties characterized by good yield stability were different, so in variety selection we have to take both genetic traits into consideration. There were positive, significant correlations among the yields of winter wheat varieties (early, middle, late), the temperature of spring months. (March-April), and the rainfall of spring months (March-April) (R2=0,703**-0,768** and R2=0,681**-0,749**, respectively). We found a high negative correlation between the temperature of early summer months (May-June) and the yields of wheat varieties (R2= -0,856**- -0,918**).
    According to the results of our experiment, it is very important to harmonize yield potential and yield stability in the variety selection of winter wheat.

  • Procurement as enterprise function at vegetable and fruit processing companies
    228-235
    Views:
    151

    The first main element of the supply chain is procurement; which has a great influence on the quality of products and services. The most important role of the procurement is the purchase of inputs which are necessary for the operation of companies. This includes procurement planning and the development of procurement strategy, so procurement is a process that is even more than simply buying raw materials. The functions of procurement are the selection of suitable suppliers, contracting, controlling and improvement of suppliers, and coordination of activities with other parts of the company.
    Planning of procurement, strategy formulation, supplier selection and evaluation, monitoring of the procurement process, and modification of original plan are important tasks of entrepreneurial procurement. The importance of procurement in a business organization is reflected by its position in the hierarchy of the firm. There are subjective and objective methods to select suppliers accomplishing their tasks on time and with good quality. Efficient purchasing management activity contributes to an increased competitiveness of the enterprise. The aim of this publication is to describe the procurement activities, to examine supplier selection and evaluation methods, to determine the position of procurement in the organization, and to prove the strategic importance of procurement.

  • The effect of the dates of shoot selection and shoot tip removal on the growth of William’s pear trees
    65-70
    Views:
    273

    Increasing the intensity of production in the case of pear can be the solution to satisfy the market demand forr high-quality fruit. The aims in this technology are the canopy treatments and the maintainance of consistently high quality yields in the long run. The experiments were performed with cv. William’s pear, a cultivar grown on large areas in Hungary. To create the optimal canopy shape for earlier fruiting, we performed shoot selection and the cutting back of shoot tips. Our results show, that both pruning methods – applied at 3 different dates – decreased the number of short fruiting parts (spurs), and increased the number of vegetative shoots. The sole exception was from this the effect of shoot tip removal when done at the earliest date.

  • Gene resources for improvement of drought tolerance and yield quality in dry pea breeding
    105-111
    Views:
    247

    The effectiveness of selection for improved drought tolerance and consumption quality in the progeny of crosses between pea cultivars with semi-leafless (afila) and normal leaves and different origins, respectively, were investigated. After single crosses, parent cultivars and F1, F2 and F3 generations were grown under non-irrigated conditions in the same trials. We created a colour scale from 1 to 9 to measure statistically the shade of seed colour. The tolerance of genotypes against high temperature was measured by the number of pods per plant. The 3:1 segregation
    observed in the F2 generation of crosses between semi-leafless and conventional cultivars indicated that the semi-leafless character is determined by a recessive gene. In contrast, the ratios of conventional (Af) and semi-leafless (af) genotypes were 7:1 and 9:1 ratio in the progenies of crosses of Af × af. The genetic progress was effective for improving the seed quality in F3 generation from crosses Af x af where we found that multiple
    dominant alleles controlled the orange colour of cotyledons and its high heritability (h2 A=0,63). Selection is more effective in producing the genotypes with high yield and normal leaves if the crosses were made between the western European cultivars such as semi-leafless Profi and Delta used as maternal cultivars and conventional Auralia cultivars. In this case, there were decreases in the consumption quality, such as seed size and shade of colour. The selection based on the seed weight of single plants for increasing drought tolerance seemed to be more effective in F4
    strains with normal leaves originated from Czechoslovakian maternal cultivar Y228; however, the genetic progress in the improvement of seed size and colour quality was slow. 

  • The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
    135-140
    Views:
    361

    Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.

     

  • Modelling forestation alternatives
    35-41
    Views:
    609

     

    Agroforestry systems are part of the history of the European Union rural landscapes, but the regional increase of size of agricultural parcels had a significant effect on European land use in the 20th century, thereby it has radically reduced the coverage of natural forest. However, this cause conflicts between interest of agricultural and forestry sectors. The agroforestry land uses could be a solution of this conflict management. One real – ecological – problem with the remnant forests and new forest plantation is the partly missing of network function without connecting ecological green corridors, the other problem is verifiability for the agroforestry payment system, monitoring the arable lands and plantations.

    Remote sensing methods are currently used to supervise European Union payments. Nowadays, next to use satellite imagery the airborne hyperspectral and LiDAR (Light Detection And Ranging) remote sensing technologies are becoming more widespread use for nature, environmental, forest, agriculture protection, conservation and monitoring and it is an effective tool for monitoring biomass production.

    In this Hungarian case study we made a Spatial Decision Support System (SDSS) to create agroforestry site selection model. The aim of model building was to ensure the continuity of ecological green corridors, maintain the appropriate land use of regional endowments. The investigation tool was the more widely used hyperspectral and airborne LiDAR remote sensing technologies which can provide appropriate data acquisition and data processing tools to build a decision support system.

  • Changing of yeaning rates using estrus synchronization and cervico-uterinal artificial insemination in goats
    43-47
    Views:
    236

    Breeding aims can be more efficiently and faster achieved by using AI. AI allows higher selection intensities for male selection, adequate genetic evaluation with efficient buck using and profitable farming. Moreover, the AI has very important advantages in animal health, especially in goats. By using our AI technology, these benefits can help the farmers in the practical life.

  • Gene Bank Developed by Induced Mutation for Selection
    45-49
    Views:
    254

    Heterosis breeding in maize caused gene erosion by using uniform inbred lines. In order to strengthen the genetic base, we established a gene bank containing lines with broad genetic variability, resistance and adaptability. The maize gene bank is a result of our work in the past two decades.
    The gene bank originated from treatments of maize seeds of hybrids and inbred lines with fast neutrons. The 1500 maize lines have great genetic variability which can be exploited after strict assessment and selection. As a result of the past several years, P 26, P 61 and P 62 lines have been released after DUS investigation in 2001.

  • Stress Response of four Common Carp (Cyprinus carpio L.) Varieties with Different Genetic Background
    26-29
    Views:
    188

    Four different carp varieties were tested for stress reaction, with the help of 10 °C cooling and 100 g/l crowding. The selected varieties represented a broad genetic spectrum of carp species. The stress was monitored by measuring plasma cortisol, glucose and lysozim levels. In each test different varieties showed significantly different reaction to stress. This means that they differ in the trait of general resistance. Thus, this trait can be used in selection programmes and the acquired information helps to understand fish-environment interaction and specific resistance. These traits should be used in selection to improve the breeding of new more tolerant species for intensive farming or for organic production.

  • Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
    51-55
    Views:
    299

    Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
    Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
    In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
    There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
    Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
    In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase

  • Nest-site selection of Hooded Crow (Corvus cornix) in urban environment
    35-39
    Views:
    394

    In the past decade Hooded Crows showed a significant growth in Debrecen. The aim of this study was to become acquainted with Hooded Crows nest-site selection in urban circumstances. The research revealed that Hooded Crows are not choosy when it comes to selecting the species of the trees, but we noticed differences regarding nesting height - Hooded Crows living in the city build their nests in higher regions than those living in places outside Debrecen. We discovered a significant difference between nesting heights and the tree species, which is probably due to the different characteristics shown by the given tree species. As for nesting heights, we found that in typical urban habitats there were no relevant differences shown. Samet the situation, when we compared these habitats in pairs, it came to light that nesting heights - when comparing lonely trees - wood segments and tree raws – wood segments - did not show significant differences too, which can be explained by the various conditions provided by the habitats mentioned.
    To sum up, during our research the following results emerged:
    1. Hooded Crows prefer approximately the same nesting heights in all kinds of habitats, urban environment and tree species.
    2. Nesting height depends on the tree species but independent from habitat.

  • Selection of Sport- and Racehorses
    3-10
    Views:
    310

    The utilisation of the horse has changed from time to time in response to human needs. For a few decades, it has been serving in several equestrian sports more intensively. It has also been proved that the standards for this kind of performance cannot be established in the way certain characteristics, such as the weight gain or milk production of other animal breeds can. Breeding horses for sporting comprises highly complex selection criteria.
    Some of these (e.g. external features, temperament, manageability and intelligence) do not put the breeder in a difficult position, but finding the traits that establishes the safety of sporting achievements poses a genetic problem.
    The performance of a horse for sports is a highly complex feature, which cannot easily be assessed or put down in figures. In addition, man plays a decisive role in shaping all kinds of performance of a horse at any given time by not only creating conditions for a better performance, but also by playing an active role in increasing it.
    The performance of the horse is mostly defined by its general aptitude to movement, ie., the regularity, clear rhythm and springiness of basic types of strides, as well as the ability to move in a naturally balanced way. Training and riding principles are based on these traits. These two together will determine about 70% of the value of the horse and its adequacy for high performance equestrian sports. In order to avoid subjectivity in determining the above variables and to increase the degree of objectivity, competent expert teams (trainers, judges, other riders) are employed to form an opinion on an individual animal.
    Assessing horse performance outside races does not seem to be efficient, as owing to the dominant effects of the environment, the indicator of inheritability is hardly above 0.1.
    Free jumping is an especially appropriate means for assessing a horse’s readiness and ability to move in an environment free of disturbing factors. In free jumping, it is especially important to judge the style of the jump. The first phase of jumping – as a sequence of movements – lasts from the moment the fore-feet touch the ground until the moment the hind-feet push off, while the second phase lasts from this moment until touching the ground. The most important task in the first phase is to make the angle of the dip of the body by the supporting fore-feet that is necessary for the jump. The quality of the jump is determined by the jumping and adequately expanded hind-legs. The intensity of pushing off and jumping done by the hind-legs can be inferred, and differences between individuals can be discerned from the shaping of the curve by the hocks and the paths of the pasterns in relation to the withers.

  • New Integrated Breeding Evaluation Method Used for German Warm-blooded Horses
    3-6
    Views:
    227

    In Germany, the new integrated breeding value estimation is based on a multiple-trait animal model, considering simultaneously information from performance test of stallion on station, performance test of mares as well as competition results of sport horses. The objective of this study was to estimated the genetic parameters of the traits used in the integrated breeding evaluation including all German warmblood breeds. The analysed data consisted of 4527, 40670 and six million records for performance tests of stallions, mares and for competition results, respectively. Genetic parameters were estimated with a multivariate BLUP animal model. Heritabilities for traits obtained from stallions tested on station ranged from 0.33 (jumping under rider) to 0.51 (trot) and for the traits from mare performance test from 0.27 (walk) to 0.38 (trot). Genetic correlations between corresponding traits recorded on performance test of stallions and mares ranged from 0.87 (canter) to 0.98 (free jumping). Heritabilities of competition traits from horses (build up for sport) were estimated as 0.12 and 0.11 for dressage and jumping, respectively. The use of all traits for estimation of genetic values of horses is expected to reduce pre-selection and optimal combines all information resources based on the estimated genetic parameters.

  • Advancement of a Common Wheat (Triticum aestivum L.) Selection System
    8-11
    Views:
    209

    All the research in Hungary and other countries in Europe focus on improving the quality of crops and increasing the competitiveness of production.
    In this respect, we have to advance the conventional technological elements, reduce the application of pesticides and fertilizers, and produce new varieties suitable for environmentally-sound production. In our crop breeding programs, we applied conventional and biotechnological methods (embryo rescue and double haploid methods) in order to get somaclonal and gametoclonal variations.
    We produced winter wheat lines (HP-31-95, HP-82-96) by traditional way, which have high baking qualities and high nutrient efficiency. Some diseases can limit the quantity and quality of a wheat crop. We examined several wheat diseases in our winter wheat candidates, and we found that our progenies have resistance to leaf rust.
    With respect to a serious problem was the small quantity of applied fertilizers and the other externals, our research focused onto advance a common wheat selection system, with the help of it, we can handle these problems. We have summarized that our selected progenies can compete with the registered varieties with quality and environmental respect.

  • Development of a New Maize (Zea mays L.) Breeding Program
    25-30
    Views:
    338

    Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.

  • Preliminary test: Evaluation and selection of tomato (Lycopersicon esculentum Mill.) varieties resistant to drought and powdery mildew
    33-37
    Views:
    343

    Powdery mildew (Leveillula taurica) and (Oidium neolycopersici) are two harmful fungi that invade the tomato (Lycopersicon esculentum Mill.) plant and grow in dry conditions. Under the influence of polyethylene glycol 6000 at a concentration of 12%, the total seedlings fresh weight, total seedlings dry weight, seedling growth rate of one seedling, seedling length, and tissue water content percentage for three tomato varieties were assessed. Despite the superiority of the (Mobil) tomato variety in terms of numerical values, the results revealed no significant differences between varieties. As a result, (Mobil) has greater vigor under environmental drought stress of lower osmotic stress than other tomato varieties. More work is required to evaluate the research selection of varieties resistant to biotic stresses in dry areas, such as powdery mildew disease.

  • Brief overview of the polymorphism analysis of genes affecting pig prolificacy (LEP, PRLP, ESR BF, EGF, FSH-β, H2A.Z)
    5-10
    Views:
    466

    Researches are being performed around the world to increase swine prolificacy by using marker-assisted selection (MAS). The present study processes researches of polymorphism examinations on 7 genes. The result of the experiments showed that the leptin gene (LEP) prolactin receptor gene (PRLP), estrogen receptor gene (ESR), properdin B (BF) epidermal growth factor (EGF), follicle-stimulating beta gene (FSH-ß) and Z member of the H2A histon family gene (H2A.Z) and their alleles have a positive effect on reproductive characteristics of different swine breeds. In addition to this, leptin gene (LEP) influences the build, meat production and growth of body fat. Further studies are concerned with the polymorphism of an increasing number of genes, which enables a faster genetic development of swine breeding.

  • Búzalisztek minőségének becslése tészta nyújtás-szakítás alapján állományvizsgáló műszerrel
    38-44
    Views:
    174

    Literature experience was utilised to develop a method for the extensograph analog testing of paste with a QTS25 Texture Analyser. 54 wheat flour samples from the same variety from variety-preservation experiments at the Szeged were tested with this method. A study was made to establish whether the method is suitable for the estimation of results derived by traditional classification (trial baking, farinograph and gluten tests).
    A close and significant correlation was found between the hardness and area data measured with a QTS25 micro-extensograph, the farinograph values and the gluten spreading data, and also between the stretching index (BC/AC) and the water uptake capacity. These correlations correspond well with the findings from a previous experimental series on industrial flour samples. A close and significant correlation was likewise observed between the grain hardness established by the MININFRA method and all the QTS25 micro-extensograph data. In contrast with earlier QTS25 data, however a close correlation was not found between the QTS25 data and the loaf characteristics.
    With the use of micro-extensograph data, the estimation of the baking industry value has been improved through stepwise variable selection, with the inclusion of several variables. The estimation of loaf volume could also be solved by means of stepwise variable selection with the inclusion of QTS25 and MININFRA data.

  • Study on the cold tolerance of maize (Zea mays L.) inbred lines in Phytotron
    41-45
    Views:
    353

    Maize has come a long way from the tropics to the temperate zone. In the beginning, the spreading of maize was prevented by its sensitivity to cold. Improved cold tolerance at germination is one of the most important conditions for early sowing. The advantage of cold tolerant hybrids is that they can be sown earlier, allowing longer growing seasons and higher yields, due to the fact that the most sensitive period in terms of water requirements, flowering, takes place earlier, i.e. before the onset of summer drought and heat.

    In Martonvásár, continuous research is carried out to improve the cold tolerance of maize. In the present experiment, the cold tolerance of 30 genetically different maize inbred lines was investigated in a Phytotron climate chamber (PGV-36). The aim of our research is to identify cold tolerant lines that can be used as parental components to produce proper cold tolerant hybrids and/or as sources of starting materials for new cold tolerant inbred lines. After observing and evaluating changes in phenological traits under cold-test, the results of the cold-tolerance traits of interest have been used to highlight several inbred lines that could be good starting materials for further research on genetic selection for cold tolerance.

  • An Analysis of Rotational Line Mating Using Computer Simulation
    35-39
    Views:
    424

    In a simulation examination, we analyzed the effect of the family size and the rate of pairing on the survival of rare genes, to keep the level of variation of the genepool and to avoid the loss of alleles.
    The population size was 360 animals. In the simulation, we calculated on the basis of a discrete population. We placed the 360 animals into different clusters, with 3 types of frequencies of alleles and 3 types of groups. We assumed 2, 3 or 4 alleles in 8 loci. We generated 15 generations using the same mating and selection system used in practise. The simulation was written with Scilab 2.7.2 software, and evaluated with SPSS software.
    There were significant changes in the effect of family size on the genetic variation in the following cases: when the base population had the same gene frequencies in all loci, and when the gene frequencies were between 0.125-0.75. In these cases, we found that the smaller families (10 animals/cluster) were better than the larger families (30 or 90 animals/cluster). The first generation where there accured a loss of alleles was averagely earliest in larger families (90 animal/cluster). This average was 3.37 generations. When we are searched the effects of the different rates of pairing we found those cases most favourable when the ratio of males and females was 1:2 or 1:4 as compared to 1:9. The first generation where there was a loss of alleles was averagely earliest at the ratio of pairing male and females of 1:9 (the mean was 3.05 generations) when the frequency of the rarest allele was 0.0069.
    The recently introduced rotating-random mating system is an eligible method for small populations for the preservation of genes.

  • The significance of biological bases in maize production
    61-65
    Views:
    314

    The comparative trial has been set up in the Demonstration Garden of the Institute of Crop Sciences of the University of Debrecen, Centre for Agricultural and Applied Economic Studies, Faculty of Agricultural and Food Sciences and Environmental Management in 2012, with 24 hybrids with different genetic characteristics and growing periods. The soil of the trial is lime-coated chernozem, with a humus layer of 50–70 cm.

    The weather of the trial year was quite droughty; the monthly average temperature was 3–4 oC higher than the average of 30 years. High temperature, together with lack of precipitation occurred during the most sensitive phenophases of maize (flowering; fecundation, grain saturation).

    The following characteristics have been observed: starting vigour, date of male and female flowering, plant and cob height, dry-down dynamics during maturation and the change of yield composing elements has also been quantified. The yield was recalculated to 14% moisture content grain yield after harvesting.

    The beginning of the growing period was advantageous, therefore the analysed hybrids could grow a high (above 300 cm) and strong stem. The yield of the hybrids changed between 10.33 and 11.87 t ha-1, but as a result of the unfavourable climatic extremes, their genetic yield potential prevailed only at a rate of 30–40%. However, moisture content by the time of harvesting was good despite its early date (12th September); it remained under below 14% in most cases. Dry-down was measured on a weekly basis between 14th August and 5th September.

    The analysis of the qualitative parameters of the maize hybrids (protein %, oil % and starch %) resulted in significant differences. The most significant difference has been observed in the case of protein content (LSD5%=2.01). Oil content was the most advantageous in the case of hybrids belonging to the mid-late growing group (FAO 400). The X9N655 and 36V74 hybrids had the highest oil content (around 4%), while hybrids P9915 and 37F73 had significantly lower oil content. Starch content was above 70% in the case of every hybrid.

    Hybrid selection is highly important in terms of yield and yield security of maize, as well as the application of modern biological fundamentals and hybrid specific technology for the improvement of the level of cultivation technology.

  • Application of GIS, precision agriculture and unplugging cultivation in plant breeding of Karcag
    49-56
    Views:
    367

    In the last two decades, the prevailing ecological conditions and climate change have caused negative effects. Therefore, a paradigm shift is needed in the field growing of plants. The latest inventions, digital technologies, precision cultivation are not enough, the mentality of the farmers is more important. For this reason, not only big financial sacrifices, but adequate receptivity are needed on behalf of farmers. Adequate skills and continuous self-education are necessary. The yield of plant growing farms is determined by ecological conditions to a 40% extent, genetic background of the seed has a 30% share and the applied agricultural technology has a proportion of 30%. In different agroecological conditions, bred varieties of plants have bigger tolerance to unfavorable factors of the regions and significant yield stability. Farmers, who buy and sow seeds, can only contribute to the genetic potential of the seeds with cultivation technology. Plant breeding provides stable genetic background and good quality seeds. Breeding activity – choosing variety proposants, breeding them, selection work, classical breeding process for 8-10 years – must create new landraces, which can produce balance, high yield and have good quality parameters in extreme ecological conditions, yearly excursion and have higher tolerance to unfavorable factors of the region giving significant production stability for farmers. In Karcag GIS technology, precision cultivation elements and soil-friendly agrotechnical methods have been introduced which largely support the aims of breeding and can also provide optimal cultivation conditions in extreme years. Because of the specificity of breeding the main aim is not only to increase yield but to provide harmonic growing for bred materials, to decrease the number and the cost of cultivation and to be punctual. In this study, applied new methods and technologies are introduced.

  • The effect of keeping technology on the microbiological status of raw milk
    67-75
    Views:
    530

    The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,
    deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.
    The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.
    During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of  these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.
    The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and therefore
    equipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk.