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The irrigation influence under the soil, microclimate and plants in maize from Crişurilor Plain
180-186Views:94The paper is based on the researches carried out in the long term trial placed on the preluvosoil from Oradea in 1976, for establishing
the soil water balance. In the irrigated variant the soil moisture was determined 10 to 10 days for maintaining the soil water reserve on
irrigation depth (0 – 50 cm for wheat and bean, 0 – 75cm for maize, sunflower, soybean, sugarbeet, potato and alfalfa 1st year and 0 – 100
cm for alfalfa 2nd year) between easily available water content and field capacity. Thus, an average irrigation rate of 2560 m³/ha was used
in the 9 experimental crops. The average of the annual rainfall for the 1976 – 2008 period was of 625.0 mm. The technologies used were
correlated with the needes of the crops, such as melioration crop rotation, chemical fertilizers in accordance with the chemical export on the
yield, manure (40 t/ha) was used in potato and sugarbeet. After 33 years of the irrigation use the soil structure degree (38.62%) did not
decrease when compared to the unirrigated maize – wheat crop rotation (37.01%). Bulk density, total porosity, penetration resistance and
hydraulic conductivity have worse values than the ones in the unirrigated variant. The humus content is very close to the humus content
determined in 1976, the phosphorus and the potassium content increased very much in comparison with the initial content (117 ppm vs 22.0
ppm); (180.0 ppm vs 102 ppm). The use of the adequate fertilization system and of the irrigation water with a good qaulity did not determine
a decrease of the pH value of the soil. The irrigation determined the improve of the microclimate conditions, the increase of the plant water
consumption, yield gains very significant statistically and higher protein content of the maize grains. -
Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:93We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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Using research findings in precision maize production
227-231Views:215The effect of crop production factors on maize yield are examined on chernozem soil in a more than 30 year old long-term experiment on the Látókép Experiment Site of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen. The aim of research is to evaluate the effect of fertilisation, cultivation, plant number, genorype and irrigation. The analysis of the data in the database of the examined period makes it possible to evaluate the effect of maize yield, as well as that of the crop production factors and the crop year, while the correlations and interactions between these factors were also examined. During the examination of the cultivation treatments, it was concluded that the highest yield was obtained as a result of autumn ploughing, but its effect largely differs in the irrigated and the nonirrigated treatments. Based on our examinations, strip cultivation should be applied periodically (e.g. strip – strip – ploughing – loosening) in areas with favourable soil conditions free from compacted layers.
In years with smaller, average precipitation supply or when the precipitation was higher than average, higher plant numbers were more favourable. Under drier conditions, but especially in several consecutively dry years, a lower plant number can be recommended which is not higher than 60 thousand per hectare. In the case of favourable water supply, 70-80 thousand plants per hectare can be used. The yield increasing effect of fertilisation was significant in the case of both non-irrigated and irrigated conditions, but it was much more moderate in the non-irrigated treatment. The extent of weed coverage was significantly affected by the previous crop. In the case of a favourable previous crop (wheat), the weed coverage was significantly lower than after an unfavourable previous crop (maize). In the case of the same previous crop (maize), the extent of weed coverage was mostly determined by the crop year and the extent of precipitation supply. Irrigation is not enough in itself, because if it was not accompanied by intensive nutrient management, yields started to decline.
The results of researhc, development and innovation contributed to the technological method which makes it possible to apply locally adjusted sowing seed, fertiliser and pesticide in a differentiated way, as well as to change the method of operations within the given plot. -
Study of alternative oxidase as possible molecular marker for phylogenetic analysis of the Botrytis cinerea
127-132Views:126Botrytis cinerea (teleomorph Botryotinia fuckeliana (de Bary) Whetzel) is able to attack several economically important plants causing gray rot. Botrytis cinerea species complex includes two cryptic species (B. cinerea and B. pseudocinerea) that tolerate fungicides differently. On the basis of classical taxonomic markers, the two related species are very difficult to be distinguished; therefore, their separation is usually performed using molecular methods based on the time-consuming molecular analysis of several markers. Our goal was to find markers, which are suitable for the differentiation. Testing the nucleotide sequences of the alternative oxidase encoding gene, B. cinerea and B. pseudocinerea strains were clearly differentiated. Moreover, the analysis of the protein sequences of the enzyme with the maximum likelihood method reflected well the taxonomic relationships of the different fungi.
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Effect of sowing technology on the yield and harvest grain moisture content of maize (Zea mays L.) hybrids with different genotypes
17-22Views:130From the aspect of the efficiency of maize production harvest grain moisture content shall be considered beside the amount of harvested grain yield. Hybrids with different genotypes and vegetation period length lose their moisture content different that is affected by row spacing and plant density – among agrotechnical production factors – depending on the given crop year. In the present research work three crop years with different weather conditions were studied (2013, 2014, and 2015). The small-plot field experiment was set up at the Látókép Field Research Centre of the University of Debrecen, Centre for Agricultural Sciences with four replications on a chernozem soil type. The effect of three factors was analysed in the experiment on yield amount and its moisture content. Factors were row spacing (45 and 76 cm), plant density (50, 70 and 90 thousand plants ha-1), while hybrids were of very early (Sarolta: FAO 290), early (DKC 4014: FAO 320, P 9175: FAO 330, P 9494: FAO 390) and medium (SY Afinity: FAO 470) ripening.
In the crop year of 2013 the highest yield was produced – regarding the average of the hybrids – by the application of a row spacing of 45 cm (4.5%, 673 kg ha-1), however there was no significant difference between the yield of the populations of different row spacings. Significant difference (14.9%, 1751 kg ha-1; 6.3%, 583 kg ha-1) could be found in case of yield between different row spacing applications in 2014 and 2015. The effect of insufficiently distributed low amount of precipitation and lasting heat days in 2015 could be revealed in yield amounts and harvest grain yield moisture content results that were lower than in the previous years. In 2015 grain yield moisture content varied between 10.3 and 13.9% in case of a row spacing of 45 cm, while by 76 cm between 11.0 and 13.9%.
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Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times
5-12Views:108Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.
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The effect of irrigation on the yield and quality of maize (Zea mays L.) hybrids
143-147Views:168In this study, the effect of water supply on the quality and productivity of different maize hybrids was observed.
Maize production is influenced by many agro-technical factors such as nutrient supply, plant density, environmental factors, water supply and temperature. Good soil quality and adequate technology significantly reduce the unfavourable effect of crop year. The impact of fertilization, crop rotation, irrigation and plant density on maize yield was greatly affected by crop year and crop rotation. The main constituent (69–75% dry matter concentration) of maize seed is starch, however, its increase can be achieved only to a limited extent, as increasing the starch content will reduce other parameters, especially protein content. Significant nutrient content improvements can be achieved by appropriate hybrid-site connection and the use of adequate technology. Protein content reduction with optimal water supply can be positively influenced by the appropriate nutrient supply for the hybrid.
The experiments were carried out at the Látókép Experimental Site of Crop Production on calcareous chernozem soil. The weather of the examined year, which was partly favorable for maize and partly unfavourable in other respects, was also reflected in the development of maize and yields achieved.
In 2019, we were examining the hybrids of Kamaria (FAO 370), P 9903 (FAO 390), DKC 4351 (FAO 370) and KWS Kamparis (FAO 350–400). Due to the dry soil condition, sowing was delayed, however, the hybrids emerged ideally because rain arrived soon after sowing, which facilitated initial development. Young plants evolved rapidly and dynamically in the case of all hybrids. In terms of heat-demanding bread, the month of May was unfavourable to temperatures below the annual average, but the higher amount rainfall helped the development. However, due to the drier period in early July, 25–25 mm of irrigation water was applied to half of the experimental areas on July 1 and 15.
The aim of our research was to determine the best population density for hybrids under favourable soil conditions (calcareous chernozem soil). In both irrigated and dry conditions, a crop density of 75,000 crops were the most favourable for the Kamaria hybrid. The P9903 hybrid in the case of a crop density of 85 crops proved to be ideal and the DKC4351 had an optimal population density of 95,000.
However, it should be taken into consideration that, in the case of soils with poor water management, the drought sensitivity of the crop stand may increase at a population density of 95,000.
As a result of irrigation, yield increased and the difference between the examined plant numbers decreased. The yield growth was relatively moderate (341 kg ha-1 – 1053 kg ha-1), which makes the economicalness of irrigation doubtful in the given year.
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Changes in the Sulphur Content of Winter Wheat in a Field Experiment
85-88Views:79The use of superphosphate as P-containing fertiliser decreased in the last years in many countries in accordance with strict air pollution laws, and the S-deposition decreased from the atmosphere to the soil as well.
Winter wheat is the one of the S-demanding plants. Recently, the gradually increasing S absence endanger the formation of required average yield of winter wheat, and has bad effect on its quality.
We examined the effect of treatments on the sulphur-, nitrogen content and the N/S ratio of winter wheat in the whole upperground plant and in the grain and straw at harvest in a arable land sulphur fertilization experiment on brown forest soil (Agricultural Company of Felsőzsolca).
We analysed the samples from spring to harvesting, in the critical phenophases. In this study we discuss only the values from the stooling and stalking and the results of analysis of grain and straw in the harvest.
We experienced that the concentration of sulphur in the whole upperground parts of winter wheat showed increase to the end of vegetation independently of fertilization. The N/S ratio was between 8% and 12% in the beginning of the growth period in the whole upperground plant, while the ratio in the grain at harvest was between 13 and 14%. When we examined the whole upperground plant, stalk and leaf at stalking, we got the highest sulphur content in the leaf. Mostly the middle level sulphur fertilization dose (4 l/ha) increased the sulphur accumulation in the green plant. At total maturing, the greatest part of accumulated sulphur is in the grain, but then the effect of fertilization is less glaring. -
The effect of nickel-contamination, nitrogen-supply and liming on the chemical composition of perennial ryegrass (Lolium perenne L.)
85-92Views:97Plant-production is determined by many production-factors. Each of these factors became subject of research-works through the years, still we state, that studying their interaction is even more important. For studying these interactions we set up a potexperiment, within that the direct effect and the interactions of four factors was inspected: soil, nitrogen-supply, nickel-loading and liming. Experiments were carried out on two soil types with extremely different characters: one was a chernozem soil with good fertility and buffering capacity, the other was a shifting sand soil with low humus-content and buffering capacity. Nitrogensupply and liming was added on two levels, while nickel on three within 12 combinations on each soil types. Plant production was cut two times within the vegetation period. The amount of production and dry matter was weighted, fractured and their element-content was measured by an ICP-detector.
Ca-content on the shifting sand soil was determined by all three factors, however the interaction between nickel-loading and liming was also significant. Nitrogen and liming increased Cauptake, that is due to appropriate nutrient-supply and improvement of a better pH-value. On the chernozem soil nitrogen and CaCO3 also increased the Ca-content. This is caused by a better nutrient supply and a higher amount of available Ca-ions.
On the shifting sand soil nickel content was increasing parallel to higher nitrogen-dosages. In presence of higher nickelamount the nickel-content of plants was also increasing, still according to liming, this increment was different. On the chernozem soil nitrogen a nickel increased Ni-uptake. However, liming also had a positive effect on Ni-content, that can be explained by the high amount on colloids in the soil, the adsorption of Ni-ions on them and in presence of liming material the replacement of Ca-and Ni-ions.
The potassium-content on the shifting sand soil was different in each liming-combination. In combinations without nickel the potassium-content of limed and not limed combinations was on the same level. In not limed combinations by adding nickel potassiumcontent was increasing, while in limed combinations no change was observed. On the chernozem soil by adding liming material the amount of uptaken potassium was decreasing, that is due to the antagonism between Ca- and K-ions. -
Response of maize seedlings to the latent zinc deficiency under different Fe/Zn rati
125-128Views:93Zinc (Zn) deficiency is a critical nutritional problem for plants and peoples all over the world. Almost half of the world’s cereal crops are deficient in Zn, leading to poor crop yield. In this study, the effect of different Fe/Zn ratio on some physiological parameters of maize seedlings were investigated on the dry matter of shoots and roots and their ratio, SPAD index and the total length of shoots. The relative chlorophyll contents significantly decreased under increasing Fe given to the Zn deficient. The results showed that the different Fe/Zn supplies decreased the total length of shoots - ranging from 9% to 65% - by latent Zn deficiency. Corresponding to several scientific findings, it was observed that the non-optimal Fe/Zn ratio of tissues take part in the evolution of latent Zn deficiency in the case of high Fe concentration.
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White rust species (Chromista, Peronosporomycetes, Albuginales, Albuginaceae) on common weeds in Hungary
30-33Views:218The obligate plant parasite fungi in the family Albuginaceae are responsible for causing white rust diseases on weeds and they are rather common worldwide. Weedy plants with characteristic symptoms have been collected in 2014 and 2015 on location Hajdú-Bihar and Jász-Nagykun-Szolnok counties in Hungary. The determination of the species were based on the morphological characters both pathogens and hosts. Albugo candida was determined on shepherd’s purse (Capsella bursa-pastoris). Common purslane (Portulaca oleracea) is a host for Wilsoniana portulacae. The fungus Wilsonia bliti (syn.: Albugo bliti), the causal agent of white rust disease was found on redroot pigweed (Amaranthus retroflexus).
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New challenges in soil management
91-92Views:199Soil management represents two important tasks that are harmonization of the soil protection with demands of the crop to be grown on the given land under prevailing farming condition. Further goals are to preserve and/or develop the soil physical, biological and chemical condition and to avoid the unfavourable changes of the soil biological activity and the soil structure. Classical authors emphasised the importance of creating proper seedbed for plants. In the physical approach, tillage was believed to play an important role in controlling soil processes. Consequently, the period of several centuries dominated by this approach is referred to as the era of crop-oriented tillage (Birkás et al., 2017). The overestimation of the importance of crop requirements resulted in damaging the soils, which inevitably led to turn to the soil-focused tillage. Since the first years of climate change, as the new trends have raised concern, tillage must be turned into a climate-focused effort with the aim of reducing climate-induced stresses through improving soil quality.The development of soil management has always been determined by the economical background. At the same time, deteriorating site conditions have contributed to the conception of new tillage trends by forcing producers to find new solutions (e.g. dry farming theory in the past or adaptable tillage theory nowadays). Győrffy (2009) recited the most important keywords were listed in 2001 and that seemed to be important in the future of crop production. These keywords (endeavours) were as follows:− Biofarming, organic farming, alternative farming, biodynamic farming, low input sustainable agriculture;− Mid-tech farming, sustainable agriculture, soil conservation farming, no till farming, environmentally sound, environmentally friendly, diversity farming;− Crop production system, integrated pest management, integrated farming, high-tech farming;− Site specific production, site-specific technology, spatial variable technology, satellite farming;− Precision farming.Győrffy’s prognosis proved to be realistic and the efforts mentioned above have mostly been implemented. New challenges have also appeared in soil management in relation to the last decades. The most important endeavours for the future are:1) Preserving climate-induced stresses endangering soils.2) Turn to use climate mitigation soil tillage and crop production systems.3) Applying soil management methods are adaptable to the different soil moisture content (over dried or wet may be quite common).4) Use effectual water conservation tillage.5) Use soil condition specific tillage depth and method.6) Adapting the water and soil conservation methods in irrigation.7) Preserving and improving soil organic matter content by tillage and crop production systems.8) Considering that stubble residues are matter for soil protection, humus source and earthworm’ feed.9) Site-specific adoption of green manure and cover crops.10) Applying site-adopted (precision) fertilization and crop protection. Considering the development in agriculture, new endeavours will occur before long. -
Analysis of sweet corn nutritional values using multivariate statistical methods
103-108Views:240Processing large amounts of data provided by automated analytical equipment requires carefulness. Most mathematical and statistical methods have strict application conditions. Most of these methods are based on eigenvalue calculations and require variables to be correlated in groups. If this condition is not met, the most popular multivariate methods cannot be used. The best procedure for such testing is the Kaiser-Meyer-Olkin test for Sampling Adequacy. Two databases were examined using the KMO test. One of them resulted from the sweet corn measured in the scone of the study, while the other from the 1979 book of János Sváb. For both databases, MSA (measures sampling adequacy) was well below the critical value, thus they are not suitable e.g. for principal component analysis. In both databases, the values of the partial correlation coefficients were much higher than Pearson’s correlation coefficients. Often the signs of partial coefficients did not match the signs of linear correlation coefficients. One of the main reasons for this is that the correlation between the variables is non-linear. Another reason is that control variables have a non-linear effect on a given variable. In such cases, classical methods should be disregarded and expert models better suited to the problem should be chosen in order to analyse the correlation system.
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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:97Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase -
The effect of different sowing depth on the yield and yield-forming elements of maize
173-176Views:34On a global scale, maize is an important food, feed and industrial crop, with an increasing production area (Nagy, 2007 and 2021). Among the environmental impacts, extreme weathering factors caused by climate change are causing serious problems for crop stability, and maize is no exception.
Precision farming is today's most innovative agrotechnical approach, which can greatly increase crop safety and reduce costs by exploiting the genetic potential of our soils and the hybrids we use (Torres, 2012).
Sowing is one of the most important agrotechnical elements, and with good seeding we can ensure that we have all the requirements of a high yielding, high growing crop (Pepó, 2019). In the case of sowing, it is important to place the seed in moist soil to provide the optimum environmental conditions for the crop to ensure uniform emergence (Széles et al., 2020; Shrestha et al., 2018).
Precision planting is the market leading technology in precision planters in the United States, and when cooperating with them we looked for methods to optimise the depth of sowing and to monitor the effect on yield by studying the initial development of the plants. The seeder was equipped with the company's SmartFirmer soil scanner integrated into the seed drill. Automatic seed depth adjustment based on soil moisture is an exceptional solution for uniform emergence and drought protection.
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Evaluation of blue and red corn landraces
239-243Views:91Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
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A partial botanical survey of a gamepreserve
27-30Views:214Hungary is internationally well known for its high level game management. An important part of game management is indoor game keeping. In Hungary more and more game preserves were founded all over the country. The role of these game preserves to retain forest stands outside of the closed area. According to the law, in the game-preserves the game bearing capacity is not the most important parameter, because in these areas the feeding is continuous. In practice it means that in game-preserves we find a much higher density of games than in nature. This causes a relative big loading of the area (of the herbaceous and arboreal), where the game-preserves are located. Our aim is to study the ecological and management issues of forest- and game resources. The scene of these work is a game-preserve related to the Nyírerdő Corporation (Nyíregyháza).
We have done a botanical survey (plant coverage, life-form spectrum) in the three previous units and in the control area (outside of the game-preserve) as well. Our results show the difference in case of the state of herbaceous plants between areas with higher and lower game density. -
Caliometric characterisation of crop production byproducts
55-58Views:143By the decreasing tendency of the fossil energy resources more emphasis put on the usage of renewable energy resources. The consideration of environmental protection and the efforts of the European Union make current the widespread usage of renewable energy within biomass energy. One of the determinative trends of biomass energy is the direct combustion of biomass. Characteristically woody stocks are produced for this aim, but there is a considerable potential in several byproducts of growing herbaceous plants or of other processes.
In our study three byproducts of plant production have been investigated which appeared at the harvest. The Higher Heating Values of wheat, maize and sunflower byproducts have been determined by an IKA C2000 Basic adiabatic calorimeter. According to the statistical analysis of the measured data the HHV of the byproducts are different, and these values are in a negative correlation with the amount of ash in % (R2=0.873) appeared by the combustion.
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Investigation of the mycelial compatibility of Macrophomina phaseolina in the Carpatian Basin
25-28Views:111Macrophomina phaseolina is a globally widespread fungal pathogen. The fungus has a very wide range of hosts. Under optimal conditions, M. phaseolina can cause serious damage tothe host plants. In this study, the mycelial compatibility of different M. phaseolina isolates was investigated. From 2019 to 2021, 12 sunflower samples were collected from different regions of the Carpathian Basin, 9 samples from Hungary, 1 sample from Austria, and 2 samples from Slovakia. The genetic variability of the pathogen is a critical problem in plant protection. Two compatible pathogen strains can easily exchange their genetic material for each other, which can lead to the development of resistance. All collected samples (12) were tested to examine their compatibility. Isolates from all tested samples were paired with isolates from all other samples thus, a total of 66 pairings were made. During the examinations of mycelial compatibility, only 20 pairs of all possible pairings were found to be incompatible, and all others (46) were found to be compatible.
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Effect of Selenium Supplementation on in vitro Radish and Green Pepper Seedlings Germination
149-155Views:167Selenium (Se) is an essential trace element for animals, microorganisms and some other eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although there is evidence that selenium is needed for the growth of algae, the question of essentiality of Se in vascular plants is unresolved. Therefore Raphanus sativus (Se accumulator) and Capsicum annuum (non Se accumulator) were treated with 0-200 mg/l natrium-selenate. The results showed that lower (2 mg/l) concentration natrium-selenate increased the fitomass and total antioxodant capacity in seedlings.
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The effect of hybrid, nutrient-supply and irrigation on the grain moisture content at harvest and the starch-content of maize (Zea mays L.)
89-95Views:105Maize is a worldwide dominant plant. According to nowadays plant production principles it is important to investigate and optimize the site-specific nutrient-supply and other production factors, such as hybrid and irrigation, in the case of this plant as well.
At the Research Institute of the University of Debrecen, Center of Agricultural Sciences and Engineering, at Látókép the effect of nutrient-supply and irrigation on the quantity and quality parameters of different hybrids were investigated in a small plot long-term field experiment. In this paper we introduce the results regarding the corn moisture-content and the starch content of the yield.
We have chosen three maize hybrids – that have been bread in Martonvásár – for our investigations. The effect of macronutrients is investigated in this experiment on five levels. The half of the experimental area can be irrigated during the vegetation period – whenever it is needed – by linear irrigation equipment, but on the other half only the water amount originating from the precipitation can be used by plants.
In the year 2008 the hybrid affected the grain moisture content at P=0.1% level, while nutrient-supply had an effect at P=10% significance level. We haven’t revealed either any effect of irrigation or of interrelationship between production factors. It can be stated that there are differences between the hybrids on each nutrient-supply and on both irrigation levels. The grain moisture content increased parallel to the longer vegetation
periods.
The starch content of maize is mostly affected by the hybrid,
so on P=0.1% significance level. Regarding our results, it can be
stated, that the starch content shows a decreasing tendency
parallel to the longer vegetation periods. -
Q-PCR analysis of the resistance of Hungarian Botrytis cinerea isolates toward azoxystrobin
41-44Views:93The genes being in the mitochondrial DNA primarily encode the enzymes of cellular respiration. Fungicides belonging to the family of quinol oxidase inhibitors (QoIs) play on important role in the protection against several plant diseases caused by fungi. These fungicides bind to the cytochrome bc1 complex so they block electron transport between cytochrome b and cytochrome c1. This way these fungicides inhibit the ATP synthesis consequently they inhibit the mitochondrial respiration. The QoI resistance has two mechanisms. One of them is the point mutation of the cytochrome b gene (CYTB), e.g. the substitution of a single glycine by alanine at position 143 results in high-resistance. The other is the cyanide-resistant alternative respiration sustained by the alternative oxidase.
In a cell there are several mitochondria. The phenomenon when the genomes of all mitochondria in the cell are identical is called homoplazmy. If in the cell there is wild and mutant mitochondrial DNA this is called heteroplasmy. Whether the mutation in the mitochondria causes fenotypical diversity or does not depend on the dose, i.e. it depends on the percentage of the changed mitochondrials. During our work we investigated Botrytis cinerea single spore isolates which have been collected in 2008-2009 on different host plants. Our goal was to decide whether heteroplasmy influences the level of resistance. We managed to detect the change of the level of heteroplasmy, so the change the level of the resistance due to the treatment with fungicide. -
The role of leaf area and photosynthesis in optimising the sowing date of maize
5-10Views:129Our sowing date experiment took place in the Demonstration Garden of Institution of Plant Sciences, Agricultural Center of University of Debrecen, in 2012–2014. The thesis contains data of test year 2014. Our purpose, besides several other examinations, was to observe how sowing date influences leaf area index and activity of photosynthesis of maize hybrids, and how those factors affect fruiting. In the experiment we monitored the change of the leaf area index and the photosynthesis of hybrids with four different growing seasons.
Based on the results, it can be concluded that most of the examined hybrids reached their smallest leaf area with the third sowing date and with the highest yield results. Hybrid Da Sonka had the largest leaf area (4.10 m2 m-2), and hybrid DKC 4590 produced the highest yield (13.16 t ha-1)
with the third sowing date.
During testing the photosynthetic capacity, the extremely high performance of the youngest plants with the third sowing date is outstanding, which can be explained by the different ripening periods. Examination of the correlation between the photosynthetic capacity and the yield, by linear regression analysis, also proves that photosynthesis has a determinative role in fruiting.
The results obtained confirm that not only the environmental and agricultural factors in the growing season have effect on the yield, but also other factors like the leaf area index and the photosynthesis are determinative parameters, and all those factors together, modifying effects of each other, develop average yields.
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Grain yield and quality of maize hybrids in different FAO maturity groups
126-131Views:68An improvement in the quality of maize grain by increasing the level of components responsible for its biological value is possible
by using genetic means. However, a change in the genotype, together with improving the nutrient properties of the grain, also has some
adverse consequences connected with a fall in yield and in resistance to diseases.
Field experiments were conducted during three years (2003, 2004and 2005) to evaluate environmental effects on grain yield and
quality responses of maize hybrids. Twenty one hybrids of various maturity groups (FAO 150-400) were planted to achieve an optimum
(60-70 000 plants per hectare) plant populations and grown under the medium-N (80 kg N ha-1) fertilization. Environmental conditions
significantly affected maize hybrid responses for grain yield, starch, oil and protein contents, and consequently, starch, oil and protein
yields per hectare. Hybrids of flint type, which have a short vegetation period, had high protein and oil content but the yield averages
were low due to the slower rate of starch incorporation. Hybrids of the dent type have a longer growing season and more intense
carbohydrate accumulation, but low protein and oil contents. In wet years there was a higher rate of starch accumulation, while dry
years are favorable for protein and oil accumulation. Positive correlation existed between starch content and grain yield and 1000-
weight as well as between oil content and volumetric weight among tested hybrids. Negatively correlation existed between grain oil and
starch content as well as between oil content and grain yield and 1000-weight. Thus, end-users that require high quality maize may need
to provide incentives to growers to off set the negative correlation of grain yield with oil and protein content. -
Timing it right: The Measurement and Prediction of Flowering
17-22Views:63Although the model described here was developed from research in controlled environments, there is now considerable evidence that in can be applied to a very wide range of natural environments in several species. Multi-locational trials augmented by successional sowing and, if considered necessary, supplementary illumination in the field to increase daylength, can be used to estimate the values of the model coefficients: (1) to characterize germplasm collections and so predict flowering behaviour elsewhere; (2) for interpreting and understanding crop adaptation; and (3) for genetic analysis of photoperiod sensitivity. We do not yet know whether the model has any contribution to make to the understanding of the biochemical mechanisms of photoperiod and temperature responses, but at the very least, it should provide the basis for indicating the most appropriate environmental conditions, genotypes and physiological stage of the plants most suitable for such investigations.