Search
Search Results
-
Review on the fatty acid profile and free fatty acid of common carp (Cyprinus carpio)
99-105Views:443Carp or ponty in Hungarian, is considered commercial freshwater fish, which is an adaptable species in both wild and cultured conditions. Carp has high nutritional value content, favorable taste, it is rich in protein, and low in saturated fat. The nutritional content in fish is composed of many chemical constituents and influenced by many factors. One of the components that its content may be different due to internal and external factors is fatty acids, which may vary depending on endogenous and exogenous factors. The endogenous or internal factors include the genetic, size, sexual maturity, and life cycle phase. While microclimate, water quality, quality of food or diet habit, and the amount of available food or starvation are considered as exogenous or environmental factors. Freshwater fish has the ability to convert essential fatty acid into long chain polyunsaturated fatty acid like AA, EPA, and DHA. Most results showed that palmitic acid and oleic acid were the dominant SFA and MUFA in carp both for wild and farmed carp in all seasons. The PUFA for wild carp was mainly dominated by DHA, while on farmed carp by LA. It confirmed that high LA content in farmed carp was related to the diet habit. The amount of lipid and FA were changed in line with the season. Even the statistical analysis showed no significant difference, but some studies showed a contrasting result. Moreover, most obtained results acknowledged that FA tends to decrease during the spawning period. The amount and composition of FA were affected by the total lipid content. The lipid must be broken down into simpler compounds such as FA or FFA for the metabolism of fish. The result of metabolism then transported into the utilising tissue and used as energy.
-
Some Variable Traits of Broiler Genotypes
25-30Views:326One the most effective ways to satisfy the requirements of mankind for food of animal origin is the fattening of chicken. Healthy and cheap meat with low fat and high protein content can be produced rapidly and in large quantities by broilers with low feed consumption. This paper presents the production results and carcass traits of three genotypes (Ross 308, Cobb 500, Ross broiler parent) females and males at the age of 42 days.
In both sexes, we found that the average body weight of the Ross parents genotype was higher (females: 2643 g; males: 3089 g) than the
average body weight of the other two hybrids – significant difference was found only between pullets. However, the smallest difference in the
average body weight between the two sexes (446 g) was found in this genotype. In both sexes, the lowest average weight was detectable in
the case of the Cobb 500 hybrids (females: 2443 g; males: 3052 g).The feed consumption ability of the females were worse – in all but one
case (Ross pullet) – than that of the males. For one kg of weight gain - in the order of genotypes – 1.79kg, 2.08kg and 2.08 kg of feed was
used. The same values for the males were as follows: 1.81kg, 1.80kg and 1.89 kg, respectively.
Carcass traits were examined and it was found that the average grillfertig weight, the weight of whole thigh meat and thigh meat of the
Ross parents’ genotype was higher than the average weight of the other two hybrids. Significant difference was found only in grillfertig- and
whole thigh meat weight. Pullets of the Ross parents’ paternal line had worse results in the average weight of breast meat with bone and
breast meat fillet than the other two genotypes. In the case of cocks was detectable the inverse of this: the average weight of breast meat with
bone was the highest in the cocks of the Ross parents’ maternal line. -
Impact of chronic heat stress on digestibility of nutrients and performance of meat type ducks
51-55Views:370The aim of the study is to determine the effect of vitamin and mineral supplement under permanently high environmental temperature (30±1 °C) on the digestibility of nutrients, performance and furthermore the composition of duck meat in the growing period. A total of four hundred mixed sex 14 days old Cherry Valley type hybrid ducks were used for the study. Two experimental diets were formulated in the study (control and vitamin E, C and zinc supplemented diet). Based on the results the following conclusions were drown: the antioxidant defence system plays an important role in the reduction of heat stress generated lipid peroxidation process. Feed additives which have direct or indirect antioxidant effects can reduce the negative effects of heat stress on the ducks performance and meat composition. Digestibility of nutrients (Dry Matter, Crude Protein, Crude Fat) was not affected by antioxidant supplementation under chronic heat stress (30±1 °C). The performance was affected significantly by Vitamin C and E and zinc supplementation under heat stress (P<0.05). In the treated group the daily weight gain (dWG) increased and the feed conversion ratio (FCR) was improved significantly (P<0.05). The energy and protein conversion was decreased also significantly (P<0.05).
-
Brief overview of the polymorphism analysis of genes affecting pig prolificacy (LEP, PRLP, ESR BF, EGF, FSH-β, H2A.Z)
5-10Views:458Researches are being performed around the world to increase swine prolificacy by using marker-assisted selection (MAS). The present study processes researches of polymorphism examinations on 7 genes. The result of the experiments showed that the leptin gene (LEP) prolactin receptor gene (PRLP), estrogen receptor gene (ESR), properdin B (BF) epidermal growth factor (EGF), follicle-stimulating beta gene (FSH-ß) and Z member of the H2A histon family gene (H2A.Z) and their alleles have a positive effect on reproductive characteristics of different swine breeds. In addition to this, leptin gene (LEP) influences the build, meat production and growth of body fat. Further studies are concerned with the polymorphism of an increasing number of genes, which enables a faster genetic development of swine breeding.
-
The possibility of increasing at the conjugated linoleic acid content in pork
27-32Views:485The composition of fatty acids in food products is a significant factor in human health. Feeding can significantly influence the composition of fatty acids in the animal fat. We analysed the effect of feeding high CLA-content (conjugated linoleic acid) feed on the composition of fatty acids in pork. The animals were grouped according to the following: Group 1) feeding experimental, ghee-mixed feed for 76 days, Group 2) feeding the same feed, but only for 33 days, Group 3) feeding sunflower-oil-mixed feed for 76 days. Ghee contains CLA in high amount. The aim of our experiment is to analyse how the high CLA content influences the fatty acid content of pork. In the end of the fattening experiment the animals were slaughtered, then samples were taken from the loin, ham, abdomen and backfat from 10 animals from each group and analysed the fatty acid content. We found significant differences between the average fatty acid content of the samples. As an effect of feeding ghee-enriched feed, the CLA content significantly increased, compared to the control group. However, the linoleic acid and the arachidonic acid content were lower, and the proportion of fatty acids was also lower when feeding control feed.
-
Investigation of the production parameters, nutrient and mineral composition of mealworm (Tenebrio molitor) larvae grown on different substrates
129-133Views:508During the rearing of mealworm (Tenebrio molitor) larvae, the optimisation of the growing substrate has a particular importance. The application of the appropriate substrate is a fundamental pillar for intensive and safe production. The requirements for substrate include the lack of toxins, high nutrient and micro-macro element content. The aim of this study was the evaluation of the effects of different substrates on the production parameters, nutrient and mineral composition of the mealworm larvae. The experiment lasted through 14 days. 5 treatments were set up at, where the variable was the substrate. The test system consisted of 25 units (5 treatments and 5 replicates). 10 mealworm larvae per unit, (total of 250 larvae) were used at the beginning of the experiment. Regarding the substrates, our study included sweet potato (SP), may turnip tuber (MT) and may turnip leaf (ML). In addition to the plant by-products, wheat flour (WF) and wheat semolina (WS) were used as control substrates. Trace element uptake and production parameters of the larvae were determined at the end of the experiment. Regarding the production parameters, it can be stated that the wheat semolina (0.081 ± 0.005 g) and wheat flour (0.069 ± 0.007 g) substrate used as control gave the best results for the final body weight. In terms of plant raw materials and by-products, sweet potatoes (0.063 ± 0.007) can only be recommended as substrate, while may turnip tuber and may turnip leaf produced significantly lower final body weight results (MT=0.034 ± 0.002 g ; ML= 0.036 ± 0.002 g). The nutrient composition of the larvae was not affected by the substrate, these results confirmed the high protein and fat content reported in the literature. The results with the production parameters were contradicted by the micronutrient content. Larvae reared on may turnip leaf (ML) and tuber (MT) showed the highest values for most of the macro- and microelements (potassium, calcium, magnesium, iron, zinc, copper) tested.
-
Comparison of Reproductive Performance of the Roe Deer (Capreolus capreolus, L.) Among Different Regions
41-46Views:221The potential and actual number of offspring of roe deer and the difference between these figures (prenatal and postnatal loss) significantly vary in each population yearly. The objective of this study is to examine the potential and actual number of offspring, the number of losses, and to find a link between the most important biological characteristics of does (body weight – BW, condition – KFI) and the number of raised offspring on four territories on the Great Hungarian Plain.
Where the number of corpora lutea (CL) is the highest, there the losses are the highest as well, and the number of raised offspring is the lowest (region I.). Here, the rearing loss is double that of the weakest territory (region IV.). Rearing losses can be associated with the fenotype of does (BW, KFI) but environmental factors also have determinative importance. Where the number of twin-calving does was the highest, I found four times more does without a fawn than where the number of twin-calving does was the lowest. The nursing success was the best (the losses were lowest) in the region where the potential offspring (number of CL) was also the lowest, but the coverage of the habitat and the proportion of forests were largest. The food supply for the animals in autumn and winter are not enough, the structure of the habitat has to be improved as well, so that it might become adequate for game protection in extreme weather conditions.
The results have to be considered as preliminary ones. It is essential to continue and extend the research to increase the reliability of the results. -
Effect of agrotechnological treatment on nutritive value of common millet
65-69Views:398The cultivation of common millet shows an increasing tendency due to its adaptability to extreme poor circumstances. In addition, millet do not contain gluten forming proteins such as gliadin and glutenin, therefore people with gluten intolerance could consume it. The vitamin B and mineral content increase the importance of millet. The aim of our experiment was to measure the quality of flour of two millet varieties treated with different nitrogen fertilizer doses.
-
Examination of the main parameters of roe deer feed compound on two territories
13-16Views:231It is widespread that roe deer are very choosy. He needs this sorting because the micro organizms, which help the digestion of high fibre plants, are missing in his stomack, that is why they are mostly called „concentrate selectors” Hoffmann, 1985, 1988, 1989).
These animals should mostly eat easily digestable plants with high nutrition level (pulses, buds, sprouts and flowers), and they are able to do this sorting because of their small mouth size. In winter there is a lack of these plants, so the high selectivity occurs only when the feed is in abundance.
Examining the amount and quality of vegetation available on the habitat of roe deer we can identify the species which can satisfy their feed demand. It is known, that roe deer as other large ruminants, from the plant abundance prefer certain plants and plant parts while there are others which are avoided. The identification of the eaten species and the rate of their occurance in the feed is the first step to become acquainted with the interaction between animal and the surroundings. -
Impact of lameness on the milk production of ewes
5-7Views:225The aim of this study was assessing the impact of lameness on the milk production, somatic cells count and component of milk. We assess also impact of lameness on the order of entry into the milking parlour.
The experiment was carried at the farm, located in northern Slovakia. The farm keeps sheep Improved Valachian. Samples of milk were taken during two periods: May, July. It was taken 428 samples together. We recorded three groups by lameness- strong lame, slightly lame, non-lame ewes. We recorded also the order of entry of ewes into the milking parlour in milking row. The results were mathematically processed using the Microsoft Excel program and statistically evaluated by SAS.
We found significant statistical differences between months (P<0.0001) in all the above mentioned indicators. In July we recorded 26 ewes with slightly lameness and 18 ewes with strong lameness. Other ewes were non-lame. Non-lame sheep had in July the highest milk yield (356±148 ml) and the lowest decrease in milk yield from May to July (-206±131 ml) compared with slightly (317±116 ml, -223±163) and strong (319±122 ml, -219±151 ml) lame ewes. However, these differences were not statistically significant. We have not identified statistically significant differences between groups in somatic cells count (logxSCC for non-lame: 4.83±0.608 in ml, slightly lame: 4.76±0.653 in ml, strong lame 4.71±0.787 in ml). Milk composition (fat, proteins, lactose) nor changes in the composition of milk that occurred between May and July were not affected by lameness of ewes. But lameness in July affected the change the order of entry of ewes in the milking parlour in July compared with the order of entry recorded in May.
-
Comparison of chemical parameters of enzyme active and inactive malt types
161-166Views:420Nowadays there is an increasing emphasis on the use of raw materials. Typically, raw materials – in this study malt – are used in animal feeds and used in the brewing industry. However, in terms of quality (eg. high fibre content), these can be included in human nutrition, we have limited information on this possibility. The aim of our work was to compare different malt flours and examine the possibility of using malt in the baking industry. We were to investigate some of the most relevant parameters, such as dietary fibre content, crude protein content, fat content, carbohydrate content, dry matter content, moisture content, salt and energy content. In the future, we aim to conduct a research on some of these parameters with different malt types as the brewing industry uses novel ingredients different cereals, pseudocereals such as amaranth (Amaranthus spp.), oat (Avena sativa L.), quinoa (Chenopodium quinoa Willd.) in addition to the spring barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.). Based on brewing studies, malt has a high fibre and protein content. Having these advantageous qualities, malt should be part of humans’ healthy diet. Using malt flour in the baking industry can be a new direction which can lead to creating a healthier lifestyle and healthier eating habits than suggested by the WHO (World Health Organization).
-
Changes of chemical composition and nutritive values of Reed Canary Grass (Phalaroides arundinacea) in first growth
37-42Views:443Change in chemical composition and nutritive value of Reed Canary Grass (Phalaroides arundinacea) were investigated in springtime in 2004-2006. The nutritive value of Reed Canary Grass was observed between the end of April and the middle of June relating to the following parameters: crude protein, crude fibre, crude fat, ash, N-free extract, net-energy growth, net-energy maintenance, Metabolizable Protein Energy dependent,
Metabolizable Protein N-dependent.
It was also analysed whether a relationship between the climatic factors affecting the growing period as well the chemical composition of grasses and the annual weather were looked for. For the estimation of weather conditions the climate index was calculated. A correlation can be detected between the change of parameters of nutritive value and the quality of the current year. Between 2004 and 2006, the result of the analysis of nutritive value indicated a significant difference with respect to each chemical composition at the rate of P<0.01 depending on the time when the samples were taken. According to the outlined data it can be stated that the change of the value of crude protein and ash show decreasing endency agreeing the research literature. In parallel with the change of nutritive value, the amount of crude fibre and N-free extract increased. However, the value of Net-Energy maintenance revealed an alteration only in the first half of the observed period. The same alteration tendency can be detected
in the Net-Energy growth. -
Examination of Reproductive Performance of Roe Deer (Capreolus capreolus) in Hungary
33-38Views:276The objective of the research partly is to compare the reproduction performance of the populations living in different regions with regard to some special characteristics (age, condition).
When estimating the age through tooth wear and cementum-layer-counting there was a difference of 0.87 years in favour of the first one (r=0,840; p<0,001). I found cementum layers at 42% of the does in the study after examining the MI teeth.
There was lose connection between the weight (eviscerated, with head and legs) and the KFI (r=0,296; p<0,01), and for further analysis, I used only the KFI as the index for condition.
The regional average KFI varied from 0.24-0.37 in fawns, 0.82-1.73 in does, with individual extremes of 0-4.05. Within the examined regions the highest index belonged to the prime-aged does, while the 1-year-olds had a lower rate, and it was the lowest in the does older than 8 years.
The rate of fertility was between 83,3(ns)-100% as we can see from the presence of the CL. All the examined does were fertile, except in one region, while among the female fawns in two regions I only found three with active ovaries. The average number of CL was 1.5-2.13, and this varied by regions; all in all it was the highest in the 2-7-year-old group (1.96) and in the ones over 8 years (2.00!), while it was lower in the does younger than 1 year (1.90). The high fertility of the does over 8 years is remarkable.
I could examine the number of embryos in two regions during the post-implantation period, and beside 100% fertility I found significant differences among the does, which can be associated with the condition. The ratio of CL carriers and the pregnant does was 100% and 73% in the two regions, the average number of CL were 1.92 and 1.72, while the average embryo number were 1.83 and 1.36 per doe. The difference between the CL and the embryo numbers on the two regions were 5% and 21%. The difference (prenatal loss) is in connection with the age (age class) of the doe. It is possible, however, that in some cases oestrus was not followed by gestation. But in roe deer, owing to the commonly known lack of luteolysis-mechanism (Flint et al., 1994), the regression of the CL of the does that did not get pregnant takes place in December and January, so the CL found in January cannot prove a previous pregnancy, which might have been followed by an abortion.
Although it has to be proven, it seems that the number of the CL (potential progeny) can be associated with the age (r=0,418; p<0,01) and the weight (r=0,312; p<0,01) of the doe, while the embryo number (realised progeny) is influenced by the age of the doe and probably by external factors.
It is essential to continue and extend the research to increase the reliability of the results and their correlation. -
Studies on Ostrich (Struthio Camelus) - Review
15-22Views:2232Ostrich has been reared in Hungary for decades, but we have limited information on this species. The aim of this review was to provide a concise description of the ostrich (Struthio camelus) based on international studies. We were to investigate some of the most relevant parameters, such as egg, meat and skin production. In this critical review we managed to sum up the most significant features and productivity parameters of ostrich and effecting factors. So as to make an accurate description of the species we have to know its morphological, behavioural and physiological characteristics. Ostrich is a very special bird with special nutritional and environmental requirements. Ostrich products, regarding egg, meat and skin are considered luxury products not only in Hungary but also abroad. Though egg has a significant nutritional value, it is mainly used for hatching chicks. In Europe we can expect roughly 40–50 eggs from a hen yearly. Ostrich has a lean meat with high protein and low fat and sodium content, moreover it is rich in minerals and polyunsaturated fatty acids. Having these advantageous qualities, ostrich meat should be part of our healthy diet. Skin production is not important in our country, but there is a special demand for high quality ostrich leather globally. In the future our aim is to conduct a research on some of the parameters. As low production rate and embryo mortality is a great deal of problem in ostrich industry, we need to investigate the incubation environment. There is an apparent disagreement among researchers on optimal slaughter age. This is also an area for further investigation. The value of skin and effecting factors on our climate as well as adaptability of the species are also worth for further investigation together with the nutrition what differs from other ostrich breeding countries. Nowadays, there is a great emphasis placed on animal welfare too.
-
The possibilities of the development of functional dairy products
33-36Views:420Because of our new aged world’s emerging stress, over exhausting and move less lifestyle the pollution, the not appropriate food consuming and the low calories in the consumed food there are more and more the called „civilian” disorders.
Many people have diabetes and osteoporosis others fight with lung, cardiovascular system, problems and find cancers of many kinds without age exemptions.
There could be a solution in changing lifestyle. In the developed side of the world there are presence of food lines with higher content in nutritious and/or vitamins and fibers such as: kalium, calcium, selen, magnesium, plus it contains less saturated fat. Because of that there is a new word in dietetics as functional foods.
Food having more inner content and/or biological values, so healthier, are called functional foods. Those foods components picked carefully for healthiness by modern knowledge of dietetics.
One of the best raw materials for functional food is the milk. It is already healthy by itself. The Körös-Maros Biofarm Ltd. has a goal of developing and marketing, health protecting organic functional foods in hungaricum products from cured milk. The Jedlik Ányos project helps the Ltd. to achieve this goal. We just finished an antioxidant test and we check if the antioxidant in present in the milk than we check the final product of the presence of the original antioxidant and the form and amount of it.
It had been feeding, for two weeks for three herd of cows selected for age, milk output and consanguinity. All of the three herd had been feeding with basic forage. One of the herds had been getting vitamin-E in the amount of 250 mg/bwkg the other group got licopin in the amount of 200 mg/bwkg once a day each by each orally, the third herd was the control group.
After two weeks we toke a sample of every herd were processed the samples into yogurt and cheese. Than we checked the raw milk, the cheese and the yogurt for antioxidant content with HPLC method. The test ended with good results by finding a great amount of antioxidant, in not only the raw milk but also in the final product. -
Changes in the Composition of the Milk of Racka Sheep During Lactation
24-28Views:350We examined the changes in the composition of the milk of 36 Racka sheep during lactation. As milking of Racka sheep is not performed anywhere in the country, the test was restricted to the examination of milk components. However, quantity could not be measured. We analysed the fat, protein, lactose, carbamide and somatic cell count content of the milk from sampling. By means of correlation analysis, milk components were compared to the age of ewes, then the number of lactation days. With the advancing of the lactation and by analysing the milk components, we found differences between the two colour-variants of Racka sheep. Additional trials are required to decide whether the differences between the two colour-variants may be considered as constants, or can be attributable to the relatively small number of samples.
-
The trend of cowmilk yield, its compostion and the body condition during the lactation
69-73Views:419The authors examined the data of 2767 trial milkings andthe months Body Condition Scores of 479 Holstein dairy cows. The condition loss was significant between the 30-60th days. The improvement of BCS begins only after the 120th day. The change in the milk protein and milk fat content were close after the condition changing. In each period of the lactation the closeness of the relationship among the condition, the milk quantity and the milk composition were different. In the aspect of the milk quantity the most significant difference was between the 2.5 and 4 BCS cows (6.68 kg) in the last period of the lactation.
-
Microbial assessment of potential functional dairy products with added dried herbs
59-63Views:259The market of dairy products is a dynamically developing sector of the food industry. Potential, functional dairy products, made by adding herbs or spices, will have antimicrobial and antioxidant effect due to the active biochemical agents of the plant additives. Furthermore, these active components will widen the storage life of food products and enhance their organoleptic properties too. We worked out a technology for creating fresh cheeses using a gentle pasteurizing method by treating the mixture of raw milk and 1.5% fat contained in commercial milk. As herb additives, we used citronella (Melissa officinalis), and peppermint (Mentha x piperita) harvested by us and dried them via Tyndall-method in convective dryer on 40 °C for 5.5 hours per day. The drying period took three days. We bought dried citronella and mint from the supermarket, which were dried by ionizing radiation, to compare the microbiological pollution with the herbs dried by us.
The main target of this research was to create a microbiologically stable, potential functional dairy product. However, because of the bad quality of the raw milk and the gentle heat treatment we used for sterilizing bulk milk, or else, cheeses were not safe for human consumption. As a consequence, we need further studies to modify our technology and get a microbiologically stable product.
-
Evaluation of blue and red corn landraces
239-243Views:342Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
-
The father of the term ‘biotechnology’ was Hungarian: The forgotten works of Károly Ereky
107-110Views:594In the Nature, Robert Bud gave an account of the fact that the generator of the concept "biotechnology" was the Hungarian Karl Ereky who, in his book published in Berlin in 1919 entitled "Biotechnologie der Fleisch-, Fett- und Milcherzeugung im landwirtschaftlichen Grossbetriebe" ("Biotechnology of Meat, Fat and Milk Production in a Large-Scale Agricultural Farm"), disclosed his observations and new views in that regard. Later on, Ereky's two essays, published in German language again, and other contemporary German sources have been processed which have confirmed the assumption whereas – in scientific terms – the further first applications of the expression "biotechnology" can be attributed to Ereky's works. Recently, we have explored and found biographical sources and documents which had been published by Ereky, which, however, have already been forgotten, by dint of which the story of origin of the first concept "biotechnology" can as well be clarified in a factual manner.
-
Nutritive value of Timothy (Phleum pratense L.) during primary growth in 2005
61-67Views:347In this paper we analysed the change of the chemical composition and nutritive value of Timothy observed during the spring of 2005. The nutritive value of Timothy was observed between the end of April and the beginning of June relating to the following parameters: crude protein, crude fibre, crude fat, ash, N-free extract, net-energy growth, net-energy lactation, net-energy maintenance, Metabolizable Protein Energy dependent, Metabolizable Protein N-dependent. We also analysed whether a relationship between the environmental factors that affect the
growing period of grasses and the chemical composition can be detected or not. While testing for correlation, the number of days from 1st January, the amount of heat accumulation, solar radiation and rainfall were considered as independent coefficients.
For the estimation of weather conditions we calculated the climate index. The observed year can be described as a year with a rainfall above the average and abundant solar radiation. A correlation can be detected between the change of parameters of nutritive value and the quality of the current year. In 2005 the result of the analysis of nutritive value was showed a significance difference with respect to each chemical composition at the rate of P<0.001 depending on the time when the samples were taken.
According to the outlined data it can be stated that the change of the value of crude protein and ash show decreasing tendency agreeing the research literature. In parallel with the change of nutritive value, the amount of crude fibre and N-free extract increased. However, the value of Net-Energy maintenance showed an alteration only in the first half of the observed period. The same alteration tendency can be detected in the Net-Energy growth and the Net-Energy lactation. -
Effect of allithiamine on the level of hyperglycaemia-induced advanced glycation end products
41-44Views:537Diabetes mellitus is a rapidly growing public health burden in both developed and developing countries. Diabetes mellitus is accompanied by hyperglycaemia, which can cause tissue injury by several mechanisms. One of these is the formation of advanced glycation end-products (AGEs). In this study, the effect of allithaimine, a fat-soluble thiamine derivative, was investigated on hyperglycaemia-induced AGEs levels using human umbilical cord vein endothelial cells (HUVECs) as a hyperglycaemic model. HUVECs were isolated by enzymatic digestion, characterized by flow cytometer and treated 30 mM glucose plus allithaimine or thiamine or cell maintenance medium as control. Allithiamine was synthesized and purified. The structure of the synthesized and isolated compound was verified by reverse phase HPLC and MALDI-TOF. AGEs were evaluated by ELISA. Collectively, our results indicate that allithiamine can reduce level of the hyperglycaemia-induced AGEs similar to thiamine.