Search

Published After
Published Before

Search Results

  • Occurrence and damage of the alfalfa root longhorn beetle (Plagionotus floralis Pallas, 1773) in old alfalfa stands in Hungary
    82-88
    Views:
    92

    The alfalfa root longhorn beetle is a pest of small importance in old alfalfa stands. It is rarely cited in the international, national literature, or even mentioned in specialized manuels. Plagionotus floralis is common in Hungary but its population density is low. It is a polyphagous species but it has importance - regarding the references – only in alfalfa. This paper analyses data gained in old alfalfa fields at two different regions of the country (Máriabesnyő, Debrecen). Damage of P. floralis was 56% in the roots of an 8-year-old stand in Máriabesnyő. Lenghts of tunnels were between 3 and 22 cm. In spite of the tunnels the alfalfa plants were viable. There was no root longhorn beetle in the 15-year-old Debrecen stand, however it has been found former in the region. The reason for the important density of P. floralis in Máriabesnyő could be the considerable diversity and covering of flowering weeds around the field. Adults of P. floralis feed on flower pollen and nectar. As to the Debrecen area,weeds were cut regularly, so the edge vegetation was poor. Present conditions (no pest control on alfalfa fields, overuse of old and failing alfalfa stands, many untrained growers) favour the reproduction and possible damage of alfalfa root longhorn beetle. 

  • The effect of diet composition, a probiotic and a symbiotic treatment on the ileal microbiota composition of one-week-old broiler chickens
    213-220
    Views:
    129

    A healthy microbiota present in the small intestine contributes significantly to small intestinal function, including digestion, nutrient absorption and health. The current study investigated the effects of a prebiotic, a probiotic and a symbiotic supplementation on ileal microbiota composition of broilers at 7 days of age. In a total of 574 male Ross 308 day-old chickens were divided into four groups using six replicate pens and 24 chickens per pen. A maise-soybean based control diet (C), a control diet supplemented with probiotics (Broilact; Br), a control diet supplemented with symbiotic (inulin, yeast, Bacillus subtilis; Sy) and a wheat based diet supplemented with wheat bran (W) were formulated. On day 7 of life, two chickens per pen were slaughtered and ileal chymus samples were collected. For microbiota analysis 16S rRNA (V3-V4 region) gene targeted Illumina MiSeq sequencing was used. Feeding all diets supplemented increased the diversity to varying degrees compared to the control (C) diet (p=0.006). As a conclusion, all supplementation substantially influenced ileal microbiota of broiler chickens at an early age. All these results could offer some information for the future study on the relationship between early intestinal microbiota and the compounds of the feed.

  • Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
    15-22
    Views:
    253

    Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.

    Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.

    Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.

  • Microsatellite Diversity of Androgenic Black Poplar (Populus nigra)
    60-67
    Views:
    70

    Genetic variation of somatic clones (1 to 35) of black poplar (Populus nigra) developed from two anther-donor trees N-SL and N-309 was determined by five SSR primer pairs. Twenty SSR alleles were detected, the number of alleles per marker ranged from 1 to 6, with an average of 3.3 including WPMS-2 (5 alleles), WPMS-4 (6 alleles), WPMS-6 (2 alleles), WPMS-20 (6 alleles) and PTR-4 (1 allele) detected by ALF (automatic laser fluorometer). A
    dendrogram produced by SPSS11 based on the presence versus absence of SSR alleles discriminated the groups of somatic clones of N-SL from somatic clones of N-309. The polymorphic markers of WPMS-2 (5 alleles), WPMS-4 (6 alleles) and WPMS-20 (6 alleles) revealed clonal variation in 1 clone (37) out of the 6 from the N-309 tree, and three subgroups out of the 29 somatic clones from the N-SL tree (17 and 24), (2 and 14) and (10 and 15). The remaining 23 of the 29 N-SL somatic clones with uniform genetic similarity suggests a good degree of genetic stability in black poplar. Nevertheless, the new SSR-clones may provide useful new genetic resources for poplar breeding. 

  • The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
    46-50
    Views:
    173

    Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.

    The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.

    We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).

    Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.

  • Harnessing diversity in durum wheat (Triticum turgidum L.) to enhance climate resilience and micronutrient concentration through genetic and agronomic biofortification
    9-20
    Views:
    168

    Huge consumption of wheat-driven food products with low bioavailability and small concentrations of zinc is responsible for zinc-induced malnutrition and associated health complications. The contemporary durum wheat varieties have inherently tiny zinc concentrations in developing grain, which cannot meet the daily human zinc demand. Despite the fact that over two billion people are suffering from iron and zinc-induced malnutrition, various intervention measures have been deployed to reverse the effect of zinc-induced malnutrition on humans. There are evidences that agronomic and genetic biofortification approaches can increase grain yield and nutritional quality (i.e. zinc, iron, protein, and vitamins) of durum wheat to a greater extent. However, there is a lack of direct empirical evidence for which the influence of both biofortification approaches on improving human health. Application of micronutrient-containing fertilizers either in the soil or foliarly is effective in combination with NPK, organic fertilizers coupled with efficient durum wheat varieties, emphasizing the need for integrated soil fertility management (ISFM). Although genetic biofortification is a cost-effective and sustainable approach, agronomic biofortification provides an immediate and effective route to enhancing micronutrient concentrations in durum wheat grain. The application of zinc-containing fertilizers is more effective under drought conditions than in normal growing situations. Hence, this article provides a key information for agronomists and breeders about the potential of biofortification interventions to improve durum wheat yield and enrich the grain qualitative traits to ensure food and nutritional security of the ever-increasing world population.

  • Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
    61-64
    Views:
    147

    In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.

  • Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
    70-73
    Views:
    60

    Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.

  • Application of molecular genetic methods for birds (literature review)
    37-41
    Views:
    120

    Ornithology studies have been extended by molecular genetic techniques. In this paper we are dealing with the most common use of areas. Our basic aim is to give a comprehensive view of the most commonly used methods in ornithological studies, including the available results by their use. We also deal with the following areas: an essential step during examination namely the applicability of sample collecting methods, and the unique identification, also the sex determination, methodology of DNA barcoding, as well as the role of molecular methods to protect endangered species. We discussed the advantages and disadvantages of the methods, such as the current trend for each method.

  • Phytoseiid mites on conifers
    65-69
    Views:
    60

    The abundance and species diversity of phytoseiids were studied in the evergreen collection of the Corvinus University of Budapest at Soroksár from March to August 2010. 329 phytoseiid mite specimens were collected from 15 cultivars of coniferous trees belonging to the following 4 genera: Juniperus, Abies, Picea and Pinus. 6 phytoseiid mite species have been identified: Amblyseius andersoni (Koch, 1957), Amblyseius tenuis Westerboer 1963, Anthoseius bakeri (Garman, 1948), Anthoseius involutus Livshitz et Kuznetzov 1972, Typhlodromus baccetti Lombardinii 1960 and Typhlodromus cotoneastri Wainstein 1963. 
    The dominant phytoseiid species were Amblyseius andersoni and Anthoseius involutus.
    Amblyseius tenuis and Typhlodromus baccettii are new to the Hungarian fauna. Typhlodromus baccettii was found on Juniperus scopulorum 'Silver Star' and 'Moonglow' while Amblyseius tenuis was collected from Picea glauca 'Alberta Globe'.

  • The recent state of cryopreservation techniques for ex-situ gene conservation and breeding purposes in small ruminants: A review
    81-87
    Views:
    269

    The viewpoint of the recent cryopreservation techniques (CT) suggests the use of a reduced volume of cryopreservation solution, high concentration of cryoprotectants and ultra-rapid cooling and warming rates help to reduce cryo-injury and maximize the viability of the preserved animal genetic resources (AnGR). The CT had now become widely accepted as one of the best methods of choice for the ex-situ conservation of AnGR due to its high success rate recorded and no-invasive nature as compared to the conventional slow rate freezing (CSRF). Rapid advances and wide acceptability of the use of assisted reproductive technologies (ART’s) particularly artificial insemination (AI) in animal breeding had resulted in a greater loss of a large number of good quality genes in virtually almost all the native breeds of animals across the globe. Small ruminant (SR) animals are not an exception in such present predicaments situation of erosion and dilution of the valuable AnGR among the native breeds. As a result of this, 148 and 16 breeds of sheep and goats respectively have already become extinct in Europe and the Caucasus. In view of the aforementioned situation, the present review aimed at exploring some of the current states of development, roles played and potentials of CT in the conservation of SR genes and genome for the immediate and future breeding purposes for sustainable development. It basically covers; animal genetic resource, the need to conserve AnGR, tools for ex situ in vitro conservation of AnGR and recent developments in breeding and cryopreservation of SR AnGR.

    Cryopreservation is playing a pivotal role in ex-situ gene conservation of AnGR. Decline in genetic diversity among SR breed population was high in Europe and the Caucasus. There is therefore, need for improvent on current stringent measures on conservation of AnGR in this region of the world.

  • Results of fast neutron induced mutations in M1 and M2 generations of sorghum (Sorghum bicolour)
    49-54
    Views:
    78

    Due to climatic barriers, the genetic variability of Sorghum varieties decreases away from the gene centre, therefore hybrids cultivated in Hungary are restricted to a relatively limited genetic basis. This limited genetic basis results in a lower rate of genetic improvement, so the genetic basis of cytoplasm factors are to be extended. We enhanced the diversity of primary materials by fast neutron treatment. As a result of this treatment we detected sterile, deformed specimens with double panicles in the stock treated with a dose of 12.5 Gy in the M1 generation. Agronomically useful
    specimens were found in the stock treated with 10 Gy and 7.5 Gy doses, where the leaf area index (LAI) and Harvest- index gave higher values than those of the control group. In the stock treated with 5 Gy dose, variations were less significant. In the reseeded M2 generation we detected albino specimens with extreme chlorophyll defects in the 10 Gy Zádor stock.

  • The effect of immigration on some population genetic parameters of the Hungarian Hucul population
    91-96
    Views:
    125

    Population genetic indicators of the Hungarian Hucul population were calculated taking also into account the effect of imported horses.The birth year of the examined 3002 individuals ranged between 1871–2015. The last year involved in the examination was 2015, when the research was conducted on the stock under stud book control. The calculations on the population were made using the statistic indicators in the Endog programme, with and without the imported Polish horses. Figures were depicted using R software. Number of offspring of the Polish individuals was presented in tables; the distribution of the offspring per stud and per mare family, as well as the Nei’s genetic distance,were presented graphically.

  • Impact possibility of hedgerows on plant protection and on the yield of winter wheat
    95-101
    Views:
    140

    Agricultural hedgerows have various impacts on the surrounding cultivated area and on the landscape. They serve as a windbreak, help manage humidity and precipitation, and are also sources of plant species richness which can contribute to the animal biodiversity. One of their beneficial effects is the yield improvement of agricultural crops. Our investigation tries to prove this hypothesis in Hungarian circumstances in the case of several winter wheat fields near Debrecen. Regarding the results, it seems that the majority of data supports the relevant, mainly German and Austrian information, but in some cases, the correlation has not been significant.

  • From Organic to Precision Farming (Contemporary Publication)
    81-86
    Views:
    66

    The paper presents a short review of the different types of farming systems:
    Biofarming, Organic farming, Alternatíve farming, Biodynamic farming, Low input sustainable agriculture (LISA)
    Mid-tech farming, Sustainable agriculture, Soil conservation farming, No till farming, Environmentally sound, Environmentally friendly, Diversity farming
    Crop production system, Integrated pest management (IPM), Integrated farming, High-tech farming
    Site specific production (SSP), Site specific technology (SST), Spatial variable technology, Satellite farming.
    Precision farming
    It concludes that the various systems are applicable in different ratios and combinations depending on the natural and economic conditions.
    The author predicts an increase in precision technologies , the first step being the construction of yield maps compared with soil maps and their agronomic analysis. Based on this information, it will be necessary to elaborate the variable technology within the field, especially for plant density, fertilization and weed control.
    The changes in weed flora during the past fifty years based on 10.000 samples within the same fields using the weed cover method are presented.

  • Complexity of ecosystem services in agricultural fields, in particular the biodiversity
    43-51
    Views:
    112

    Since the Convention on Biological Diversity a lot of papers have been published how to measure and value biodiversity. In the last decades publications on agro-ecosystems become more frequent and play a significant role in the provision of ecosystem services. There is a uniform definition for biodiversity in general, however, in terms of agro-ecosystems and their services (including biodiversity) many weaknesses can be identified. The objective of this paper is to explore some of these problems with special regard to different definitions and terms and to the farmland ecosystem services. One solution could be to adopt a more complex system which has some ecological and environmental components (air, water and soil pollution) and also takes in to consideration the efficiency of agricultural production.

  • Additional data for the evaluation of coat colour varieties in the Hungarian Grey cattle
    44-47
    Views:
    94

    When preserving genetic resources, one of the most important tasks is to conserve as much of the given gene pool for the future generations as we can. Therefore, traits that have no economic value at the moment should also be conserved. The great variety of coat colours seen in the Hungarian Grey cattle form part of the world’s genetic heritage. In order to maintain the world’s genetic diversity, we have to maintain these varieties, as well. The different coat colour varieties were determined – in both sexes and in several age groups – with a Minolta Chromameter CR-410 in an objective way. We found that the rate of the three main coat colour varieties of calves: the light reddish, the reddish and the dark reddish were 26%, 52% and 22%, respectively. Statistically significant differences were found between the L*a*b* values of the reddish coat colour of the Hungarian Grey and the red coat colour of the Limousin calves. The rate of the crane, the grey and the light grey coat colour varieties were determined in the measured female groups. The L*a*b* values of the Hungarian Grey and Maremman bulls’ coat were compared and evaluated.

  • Changes in the genetic variability of the Furioso-North Star population between 1989 and 2019
    61-65
    Views:
    82

    The Furioso-North Star (FNS) is one of the indigenous Hungarian horse breeds, originated from Mezőhegyes Stud. The aim of this work was to analyze the pedigree diversity and inbreeding of the registered Hungarian Furioso-North Star population in two different time points: the first was the active population in 1989, the second was the active population in 2019. Pedigree data was analyzed using Endog 4.8 software. The pedigree completeness was calculated in three different ways. In the past 30 years the pedigrees became more complete. In the population 2019 were 5 horses with ancestor in the 40th generation. Only 17–17 animals covered the 50% of the genetic variability for the two population. The most important ancestor was Herod xx in both reference populations. The FNS breed has English Thoroughbred background, that might be the reason of having several horses form this breed in the database. There were six animals among the ten most important ancestors in both reference populations. The average inbreeding coefficient was 3.31 in 1989 and 4.22 in 2019. Animals with highest inbreeding coefficient were born from the mating of close relatives (typically father-daughter). The Bart Furioso III-84 Boglár had the highest inbreeding coefficient (0.299) in the population 2019.

  • Morpometric study of Hungarian honey bee (Apis mellifera L.) colonies
    59-63
    Views:
    238

    The honey bees are essential for the pollination of agricultural plants. The Pannonian honey bee, Apis mellifera pannonica, is native to Hungary, only these subspecies are being bread in our country. The parameters have been separated the pannon and italian honey bee subspecies, the colour of tergit, the cubital index and proboscis length. The morphometric analisys is of special importance because this, on the one hand, shows correlation with honey bee production and on the other hand, the pure morphometric charactersitics is the basis of any potential honey bee export. Mitochondrial DNA and microsatellites are the common methods to define genetic diversity and the separation of subspecies.

  • Some population genetics parameters of the present Hungarian Hucul Horse population
    15-22
    Views:
    150

    We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.

  • Arthropods assiciated with the stinging nettle in Hungary
    97-103
    Views:
    86

    Stinging nettle (Urtica dioica Linnaeus) (Urticaceae) is a well known medicinal plant cultivated in some European countries for a long time. Because of its multiple usability (food, medicinal plant, feed, fiber), adventageous agrotechnical qualities and low demands for plant protection, its more extensiv utilization can be expected. However, during cultivation from time to time little damages can be occurred on it. The aim of this paper is to show and estimate the most important arthropods (pests and natural enemies) of stinging nettle. Under the pests characterized in the paper according to the references the peacock and the small tortoiseshell are the most important species living on stinging nettle. Their individuals from time to time propagated can cause an important damage on nettle leaves in cultivated nettle stands or assemblages. On the base of a 12 year observation period (Gödöllő, Debrecen, 1998-2010) the following species have been observed: Psylliodes attenuata, Chrysomela fastuosa, Phyllobius pomaceus, Pleuroptya ruralis, Inachis io, Aglais urticae, Microlophium evansi, Microlophium carnosum, Aphis urticata, Dasineura urticata, Tritomegas sexmaculatus. Inachis io has been the only species which during the observation period did danger the stinging nettle stand. The other pest species have not threated even timely either the stinging nettle stand or a single plant. The number and diversity of natural enemies was rather low: running crab spiders (Philodromidae), tangle-web spiders (Theridiidae), crabbing spiders (Thomisidae), lacewings (Chrysopa perla, Chrysopa formosa), coccinellids (Coccinella septempunctata, Propylea quattuordecimpunctata, Adonia variegata), hoverflies (Episyrphus balteatus), earwigs (Forficula auricularia), scorpionflies (common scorpionfly (Panorpa communis) and European paper wasp (Polistes dominula) predominated. 

  • Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
    81-85
    Views:
    123

    The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.

    Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.

  • The system of faunacomponents conception and its application to qualify the degree of naturalness of fish assemblages
    93-101
    Views:
    63

    The increase of species richness in communities of natural biogeographic regions in an anthropogenic way can be expressed as biogeographic pollution. Where there is contaminated fauna, one must investigate how this occured. I studied how the members of Hungarian fishfauna could be present resident and have managed to workout a so-called system of fauna component conception which is suitable for this requirement.
    The expression of ’faunacomponent’ includes other expressions. That kind of species belong to a certain faunacomponent that reached the studied area in the same way. Species reached the studied area without an anthropogenious contribution are qualified as native fauna components. These kind of species are endemic species, occasional species and settlers. In contrast to native faunacomponents, some kind of human influence is always detectable in connection with the way reached the studied area by non-native species. Accordingly, group of non-native species includes directly or indirectly facilitated settlers, species that are introduced accidentally and those introduced intentionally. It is obvious that classifying a given species could be construed solely in connection with a certain area.
    Applying Shannon diversity index I have initiated the assemblage natural index (ANI) in order to show a way how to use the system of faunacomponent conception in practice. ANI refers to the degree of naturalness of faunistical samples. If ANI > 1 it means there are not any non-native species and ANI < 1 it means there are some non-native species in the sample respectively. In the latter case ANI decreases proportionally with the number of non-native species and specimens. In that special case when only one specimen of only one non-native species can be found in the faunistical sample ANI = 1.

  • Application of molecular genetic methods for species Equus caballus – Literature review
    21-28
    Views:
    152

    In this study our aim was to provide a comprihensive overview of the most commonly used methods in molecular genetic studies related to Equus caballus. Thus we are dealing with the D-loop region of mitochondrial DNA, with microsatellites and also with single nucleotid polimorphism as SNP. The advantages and drawbacks of each method were also explored.

  • Possibilities of biodiversity conservation in agricultural fields
    39-45
    Views:
    122

    The biodiversity loss is one of the biggest environmental problems in the world. The objective of this paper is to present some nature conservation practices on agricultural land. Farmlands play a significant role to preserve biodiversity because some highly protected species can only find their needs on agricultural land. The Biodiversity Strategy of the European Union (2010-2020) creates new directives to reduce biodiversity loss, preserve and improve diversity, especially on agricultural land. Furthermore the importance of this subject is that the share of farmland in Hungary is much higher (57%) than in the EU-27 on average (42%). The loss of agricultural land and the increase of land abandonment cause intensification of agricultural production leading to the loss of biodiversity.