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The effects of bioactive feed additives for the production parameters of common carp (Cyprinus carpio) fingerlings in intensive recirculating system
35-41Views:245For the appropriate quantity and quality of fish meat the pond farmers need to use up to date nutrition and feeding technologies. Recently the intensification of the carp production is stepping up with the availability the proper quality of the artificial feeds. The using of different feed additives make a better feed conservation ratio, faster growing rates and more uniform stocks.
In our experiment what is made with common carp (duration of the experiment was 8 weeks long) we compared two different kinds of feed additives in different doses. One of them contains only natural mineral elements and the other additive is a natural origin probiotics. Both additives were used in the quantity of 0.5, 1 and 2% of the total feed. By the result of the 8 weeks experiment it could be said, that the using of the humic acid based mineral elements feed additive. Makes a better FCR comparing with the control stock, but was not as good as the using of the probiotics. At the humic acid treatments the more doses, the better FCR value, but at the probiotics it wasn’t true. The 0.5% probiotic treatment gave the best result. It is concluded that the using of the probiotic feed additives always gave a better performance of the fishes (average weight, biomass, FCR) than the humic acid treatments. The using of the feed additives hasn’t got an influence for the survival rate of the carps.
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The effect of the queen's age on the Varroa mite (Varroa destructor) burden of honey bee (Apis mellifera L.) colonies
83-87Views:310An apiary trial was conducted in 2016 August to October in Szabolcs-Szatmár-Bereg County, Nyírmada to evaluate the influence of queen’s age on the Varroa destructor-burden in the treatment colonies. Sixty colonies of bees belonging to the subspecies Apis mellifera carnica pannonica in Hunor loading hives (with 10 frames in the brood chamber/deep super) were used. The colonies were treated with amitraz and the organophosphate pesticide coumaphos active ingredients. The amitraz treatment includes 6 weeks. The coumaphos treatment with Destructor 3.2% can be used for both diagnosis and treatment of Varroasis. For diagnosis, one treatment is sufficient. For control, two treatments at an interval of seven days are required. The colonies were grouped by the age of the queen: 20 colonies with one-year-old, 20 colonies with two-year-old and 20 colonies with three-year-old queen. The mite mortality of different groups was compared. The number of fallen mites was counted at the white bottom boards. The examination of spring growth of honey bee colonies has become necessary due to the judgement of efficiency of closing treatment. The data was recorded seven times between 16th March 2017 and 19th May 2017.
Data on fallen mites were subjected to one-way analysis of variance (ANOVA) and Post-Hoc Tukey-test. Statistical analysis was performed using the software of IBM SPSS (version 21.). During the first two weeks after treatments, the number of fallen mites was significantly higher in the older queen’s colonies (Year 2014). The total mite mortality after amitraz treatment in the younger queen’s colonies was lower (P<0.05) compared to the three-year-old queen’s colonies. According to Takács and Oláh (2016) although the mitemortality tendency, after the coumaphos (closing) treatment in colonies which have Year 2014 queen showed the highest rate, considering the mite-burden the colonies belongs to the average infected category. The colonial maintenance ability of three-year-old queen cannot be judged based on the influencing effect on the mite-burden. The importance of the replacement of the queen was judged by the combined effect of several factors.
During the spring-growth study (16th March–19th May) was experienced in the three-year-old queen’s colonies the number of brood frames significantly lower compared to the one- and two-year-old queen’s colonies. In the study of 17th April and 19th May each of the three queen-year-groups were varied. Therefore in the beekeeping season at different times were determined the colonial maintenance ability of queens by more factors: efficiency of closing treatment in early spring, the spring-growth of bee colonies, the time of population shift (in current study, this time was identical in each queen-year), honey production (from black locust).
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The effect of fertilization and plant protection in sunflower (Helianthus annuus L.) production
57-63Views:257Sunflower is the most important oil crop in Europe which is grown on the biggest area of all the oil crops. The area of producing sunflowers in Hungary was changing to 524–704 thousand hectares in the past decade in comparison with approximately 100 thousand hectares in the 1970’s. In our experiment different sunflower hybrids were examined. The doses of fertilizers were the following in 2017 and 2018: 0–30–90–150 kg ha-1 N, 0-50-90-90 kg ha-1 P2O5 and 0–70–110–110 kg ha-1 K2O. Three different treatments of plant protection were used in the experiment to protect them from fungal infections. A rise in the dosage of nitrogen resulted in increasing infection. The biggest fungal infection was identified in 150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg ha-1 K2O treatment. The largest infection was measured on plots without plant protection at the highest N doses, both in leaf and disc diseases. We measured the highest 41.25% in 2017 and 53.1% in 2018 Diaporthe helianthi infection, and 24.5% in 2017 and 25.5 % in 2018 Sclerotinia sclerotiorum disc infection in these plots. kg ha-1
Average yield was changing between 2.96–4.67 t ha-1 in 2017/2018. The lowest yield was obtained in the absolute control plot (without plant protection and without nutrient) in both years, which was 2.96 t ha-1 in 2017 and 3.14 t ha-1. The yields increased due to growing nutrient supply and excellent plant protection. The highest yields were 3.67 t ha-1 in 2017 and 4.67 t ha-1 in 2018 (150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg K2O active substance). Purpose of the experiment at analysing the impact of different treatments on the profitability of sunflower production.
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Comparative examination of a bacterium preparation (BACTOFIL® A10) and an artificial fertilizer [CA(NO3)2] on calcareous chernozem soil
75-80Views:134In a small-pot experiment a bacterium preparation, Bactofil® A10 and an artificial fertilizer containing Ca(NO3)2 in different dosages were studied on calcareous chernozem soil, concerning the readily available nutrient content of soil (nitrate-nitrogen, AL-phosphorus, ALpotassium content of soil, some soil microbial characteristics (total number of bacteria and fungi, cellulose-decomposing and nitrifying bacteria, CO2-production of soil), and the amount of the plant biomass.
The readily available nutrient content of the calcareous chernozem soil increased due to the treatments, except for the change in the soil nitrate-nitrogen content, which did not measure up to the control due to the effect of high-dosage Bactofil.
The treatments also influenced the examined microbial characteristics of the soil positively. The artificial treatments significantly increased the total number of bacteria and the number of cellulose-decomposing and nitrifying bacteria. The low-dosage Bactofil significantly increased the number of cellulose-decomposing bacteria and both Bactofil dosage significantly increased the number of nitrifying bacteria. The measure of the soil respiration grew in all treatments, but significantly only in Ca(NO3)2 fertiliser treatments.
The quantity of the plant biomass was grew in a low-dosage Bactofil and significantly in the artificial fertiliser treatments. The highest plant biomass quantity was measured in the high-dosage artificial fertiliser treatment.
In the correlation analyses we found some medium positive correlation between the soil chemical, microbiological parameters examined, and the plant biomass in the case of both treatment-forms.
Based on our results Ca(NO3)2 artificial fertiliser treatments on calcareous chernozem soil proved to be more stimulating regarding the
examined soil characteristics and the amount of the plant biomass, but the low-dosage Bactofil also positively influenced the majority of the
soil characteristics examined in terms of nutrient supply. -
The effect of washing for the shelf-life parameters of spinach (Spinacia oleracea L.)
131-135Views:123Spinach is a very popular green leafy vegetable because of its versatile usage and beneficial for the health. However, spinach may contain several pathogen bacteria: Escherichia coli, Klebsiella spp., Salmonella spp., Enterobacter spp., Citrobacter spp., Shigella spp. and Listeria monocytogenes, which can cause several serious health problems. This study investigates the effects of washing with citric acid for the shelf-life parameters of spinach in comparison to the effect of washing with water and control. Washing of spinach with 0.5% citric acid solution decreased the elasticity of the spinach leaves, as well as the chlorophyll content. On the other hand, the total plate count, as well as the yeast and mold count could be decreased with this treatment, but difference was not detectable at the forth storage day. The fecal indicator E. coli did not change, indicating washing was not effective in this case. Further optimisation of treatment and storage conditions may decrease microbial risk of fresh spinach consumption without decreasing its sensory quality.
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The possibilities of biomass utilization in the field of spatial development
59-63Views:92By joining the European Union, Hungary made a commitment to increasingly utilize renewable energy sources. Keeping in view the agricultural circumstances of Hungary, we can state that biomass utilization has, overall, the greatest range of possibilities to use its products as ‘energy sources’. Biomass raw materials are useful in meeting emission control regulations for environment protection and to reduce climate change. The role of biomass production in spatial and rural development and in rural employment, and also in the decrease of Hungary’s energy dependence, supports development in biomass energy use. My aim in studying biomass utilization is the creation of a micro-regional level, decentralised, agro-energetic system-model for the use of plant and animal biomass for energy purposes, whether naturally or by anthropogenic activity, on agro- and sylvicultural areas, and on animal breeding farms. This model simulation method serves as a planning base for policy-makers during the spatial planning processes. The examination is presently in the stage of data collection. The collection of the basic data of the examination area and the purchase of the necessary equipment, materials and maps has already started. Contact has been made with local stakeholders, municipalities, municipality associations and micro-region managers.
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Weed control with herbicide incorporation in sunflower
73-76Views:186During the last decade certificate registration of 13 active ingedients were removed by European Union from sunflower herbicide market, including the basis for the incorporating technology, the trifluralin active ingredient as well. Its relative, the benfluralin active ingredient, which include the Balan 600 WDG herbicide product in sunflower, will be sold again from 2015 spring in Hungarian pesticide market. It has a broad-spectrum and lond residue besides it has very high level selectivity on sunflower. It has very good effect against annual monocotweeds such as common barnyadgrass, foxtail species, large crabgrass and wild proso millet, dicotyledonous weeds such as common lambsquarters,and redroot pigweed. It has significant side-effect against common ragweed, black nightshade, wild buckweed and prostrate knotweed. The long effect residue provide the weed-free til harvest. Benfluralin is totally selective on sunflower, as no colouring, any deformation or growth inhibition was not observed during the entire growing season. It should be sprayed 3-4 days before sowing within 1 hour and to be incorporated into the soil in 4-6 cm depth with tillage equipment. It can be used in tank mix with fluorochloridon in incorporated technology against annual dicotyledonous weeds. After the Balan incorporation can be used postemergence timing imazamox and tribenuron-metil active substances against hard kill and deeply germinating weeds. The products can not be used in tank mix with bacterial products.
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Plant protection praxis on Hévíz Nature Conservation Area (Lake Balaton District, Hungary)
81-83Views:171The nature conservation park, which belongs to the Spa and St. Andrew Hospital for Rheumatics of Hévíz, is 60 ha in size. The famous Lake of Hévíz, the two overfalls and the main buildings of the hospital are located in the middle of the park. They are surrounded by protective forests, parks and gravel esplanades. During the past few decades, the population of neophyton plants and invasive insects have increased considerably. These mean serious challanges to develop efficient control methods. Special care must be taken of environmental and plant protection regulations. Keeping plant protective regulations are especially strict around natural and spa waters.
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Analysis of the plant physiological effects of late, artificial corn smut infestation using remote sensing methods
31-35Views:177In Hungary, corn is also infected by several important pathogens. In this experiment, we analysed the plant physiological effects of artificial late cron smut infestation using remote sensing methods under field conditions We examined the experimental area from which the data comes from with a DJI Phantom 4 Multispectral Drone NDVI and NDRE indices were calculated and analyzed in GIS programs. Individuals treated with a higher dose remained much greener than the untreated control. They also showed significant differences within the indices used.
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Study of the Interaction Between Common Carp (Cyprinus carpio L.) and Silver Crucian Carp (Carassius auratus gibelio B.)
16-19Views:122During the last few years, the spread of the silver crucian carp caused significant problems in pond fish culture. One of the main challenges of the successful fish production in fishponds, is to rid ponds of the silver crucian carp. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0-, 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. During the breeding season, weekly test fishings were made to examine the growth of the fishes. At the end of the experiment, the yield/hectar and the growth rates were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower productivity and growth rates in common carp. In the control pond (with no silver crucian carp), the average weight of the common carp was over 20 g and the yield/hectar was over 700 kg, while in the pond with the most silver crucian carp, the average weight of the common carp was 4.5 g and the yield/hectar was only 123 kg.
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Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program
45-51Views:115In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials.
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Land use changes in suburban areas – case study of Lublin
43-46Views:108The main reason to analyzing the space structure in the Lublin area is to determine the direction and pace of suburbanization in municipalities adjacent to Lublin, distinction factors and motives of population movements to the suburbs, complain rural-urban interaction and multifunctionality of land use. Housing development over the years was accompanied by confusion in planning documents and the law. Changes in regulations on land use in 1994 and 2003 in Poland additionally deepened the negative situation. Local authorities failed to control the spontaneous process of suburbanization, which adversely affected not only the spatial structure of municipalities, but also on local relationship, landscape, land use and the former urban systems. The result are long-term problems associated with incompatibility rural areas to support a growing number of residents, such as failure of the social and technical infrastructure.
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Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
25-30Views:255In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.
We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.
Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.
We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.
Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.
We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.
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Effects of paraffine oil on leaf and berry mycobiota on two grape varieties
61-66Views:213Application of fungicides have advantages and also some direct or indirect disadvantages, such as imbalance and/or fungicide resistance in microbe population. To avoid these problems the development of alternative, eco-friendly methods like mostly spraying with oils are in the focus nowadays. The investigations of the effects of fungicides on microbiota in some cultivations can give a more complex view to this topic and developmental possibilities. In the present study, our aim was testing of the effects of paraffine oil (as alternative fungicide) on microbial properties (CFU and rate of filamentous fungi and yeasts) of Chardonnay and Kékfrankos leaves and berries.
Our results from 2014 showed that the application of paraffine oil as sole spray agent can decrease the presence of saprophytic filamentous fungi on the berries of Chardonnay (susceptible for fungal infections). In the case of Kékfrankos berries opposite properties were observed, which may be the result of the absorption of oil by the thick wax layer of this variety. The oil treatment did not affect the yeast population of Chardonnay and Kékfrankos berries contrary to negative effect of the regular pesticide treatment. The selective fungicide effect of paraffine oil against filamentous fungal population caused the accumulation of yeast cells in the mycobiota of grape berries. The careful use of this yeasts in spontaneous fermentation can improve the aroma profile of wines. The year of 2015 did no prefer the growth of fungi, therefore no interesting properties were detected in the mycobiota of grape varieties. The occurence of the harmful saprophytic filamentous fungi predicted to be increased in mild climate agricultures as the result of the climate change.
In summary, the paraffine oils are seem to be promising tools for the eco-friendly control of harmful fungi of grapes.
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Genetic diversity study in Hungarian coldblooded horses
29-34Views:290Because of the feeding technology innovation, accelerated transport and communication facilities breeds of high performance breeds replaced local autochone breeds worldwide. These latter species however have an important role in gene conservation. Hungarian cold-blooded horse breeding stock are lacking pedigree, the actual founder breed mares are not known. For this reason, it is an major priority defining the genetic backround of the existing flock, for that breeding could operate with purposeful using of origin maternal founders. In the present study 195 cold-blooded Hungarian mares tail and mane sample were analized. Our analysis was carried out between 15531–15752 base pairs in mithocrondial DNA D-loop region, which reported a total of 222 base pairs. Fourtyone polymorphic sites were determined, which resulted in 39 haplotypes (h=39). The average pairwise differences were k=6.825. High haplotype and nucleotide diversity values were observed (Hd=0.968±0.003, π=0.026±0.003). Based on the previously defined variable positions of haplotypes defined by Jansen et al (2002), we groupped our haplotypes into haplogroups. 23 percent of the studied population (45 mares) belonged to haplogroup F1. Nearly 97% of the analyzed population was classified into one of eight haplogroups defined by Jansen.et al. (2002). This study gives genetic information nearly 25% of the Hungarian population. Another possibility would be patterning more mares or involving more genetic marker in the study which will assuming the possibility of a more comprehensive analysis.
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Economic impacts of applying EU animal protection regulations in hog breeding farms
76-80Views:120Hungary’s intention to join the EU makes it necessary to adopt, introduce and use the EU system of law. In Hungary, the legal control of animal welfare has improved (XXVIIIth law in 1998); however, most of our pig farms do not meet the EU animal welfare law requirements for some reason. We examined 9 pig farms in Hajdú-Bihar, Borsod-Abaúj-Zemplén and Heves counties. We chose those farms which use the combined breeding
technology most frequently used in Hungary. The most important part of the welfare directive is the definition of the minimum space per animal. We analysed the data in comparison with EU laws. On the basis of the analysis, it can be said that there is a narrow cross-section: the breeding of piglets. During the cost analysis, we analysed cost and highlighted the permanent cost. We studied how these costs would change if EU animal welfare laws were observed. We also examined the specific data per 1 sow and per 1 kilogram of slaughter pig. We compared the present data (1999) with those we get if EU animal welfare laws concerning minimal space per pig were now followed. It can be stated that after decreasing the sow live-stock in accordance with EU directives, the permanent cost would increase by 17,7% per sow. If the required space per sow were provided, the total cost per sow would increase by 1,9% from 421,1 thousand forints to 429 thousand forints, on average. This would mean a decrease of 7,9
thousand forints profit per sow. As with the decrease of the number of sows, the number of slaughter pigs also decreases. Total cost per 1 kg of slaughter pig would increase from 214,7 forints to 218,2 on average. -
The effect Benefit PZ biostimulant on the fruit size and yield production of apricot varieties (Prunus Armeniaca)
16-18Views:141Fruit size is determined by the genetic makings of varieties the pomology and enviromental factors. The climate and the previous year’s harvested yield have a great effect on yield volume. It is not easy to harmonize the qualitative and quantitave parameters of the fruit. High yield usually causes smaller fruits.
The aim of our trials was to evalaute the effect of Benefit PZ biostimulant on fruit size. We examined two apricot varieties (Bergeron, Magyarkajszi C235) for the efficacy of the product. The three applications were made with 400 l/ha water with 2500 ppm. The optimal interval of application with Benefit PZ is during in the early phases of development. The size of the final fruits depends on the number of cells making up the fruits. The later application could not increase fruit size growth, there are no significant differences between the Benefit PZ sprayed and the control plots. The totally harvested fruit weight was 13.5% more on the trees of cv. Bergeron, it was caused by the higher fruit set. On the sprayed trees were 12.2% more fruit. Therefore were no differences between the average fruit weight. Fruit weights of the treated trees were 44.37 g on cv. Bergeron, 44.15 g on cv. Magyarkajszi C235 and on the no treated trees were 43.83 g as well as 45.33 g. -
Sensitivity study on Virginia fanpetals (Sida hermaphrodita (L.) RUSBY) cv PETEMI to different herbicide agents
89-92Views:86The cultivation of Virginia fanpetals (Sida hermaphrodita (L.) Rusby) is a relatively new phenomenon in Europe. On the basis of the biology of the plant and the practical work implemented, it has been stated that the traditional field practice does not provide the appropriate conditions of biomass production for energetic purpose. The development of the proper weed control is inevitable for the healthy stand, as in the early phenophases the plant is growing slowly and it is exposed to weeds.
Our objective was to test some herbicide agents as no previous relevant data had been published. -
The Security System of the United Nations (A Theoretical Approach)
51-56Views:114In contemporary world, influenced by globalization, security is one of the most discussed issues. It is almost commonly accepted that security should be regulated within some kind of international system. What should be agreed on are the nature of subjects within the system, the kind of resources, ways to control it and finally, how to achieve an agreement within the system’s framework. Thus the objective of this article is to show the axiological premises that underlie normative regulations on the above issues. Significant diversion in this sphere is observed within the Organization of United Nations. The following article analyses the UN documents, with a particular emphasis on the Millennium documents, determining the long-term perspective of actions. The article shall also try to define the issues not covered in the UN regulations and determine what dilemmas UN unsuccessfully tries to tackle.
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Examining reaction of sunflower genotypes on planting time on chernozem soil
93-99Views:122We studied the effect of planting time on plant pathological factors, leaf area index and yield production by applying various fungicid treatments on two different sunflower genotypes in 2013.
By delaying planting time, both the extent of Diaporthe, Alternaria and Phoma infections decreased. The differences between the volume of infections were significant in the case of the early and late sowing time results. The application of fungicide treatments induced a notable decrease in the extent of infections for all three pathogens examined. The LAI-values varied between 0.3 and 5.6 m2/m2 in 2013 depending on the hybrid, sowing time and treatment. Stocks planted at distinct times reached maximum leaf coverage at different times. The planting time and the fungicide treatment had a significant effect on the formation of the leaf area. In the case of average and late planting times, fungicide treatments elongated the preservation of the green leaf area.
With respect to the yield amount, average planting time (27 April, 2013) turned out to be optimal in 2013 (control – NK Ferti: 4.621 kg ha-1, PR64H42: 4.196 kg ha-1; double-treated: NK Ferti: 5.282 kg ha-1, PR64H42: 5.090 kg ha-1). Fungicide treatments resulted in significant yield growth in all cases during our research.
We applied Person correlation analysis to evaluate the hybrids’ sensibility to infections and our results varied in the case of Diaporthe and Phoma (r=-0.343*, -0.379**). Infections of the three pathogens were significantly reduced by delaying the planting time and applying fungicides. Late sown stocks preserved the green leaf area for a longer period. Besides, the application of the fungicide treatment and the hybrid itself also led to the preservation of the green leaf area. However, pathogens examined notably decreased the leaf area by the end of the growing year. The fungicide treatment had a remarkable effect on yield growth (r=0.603**). Furthermore, the presence of higher LAI-values in the period prior to August also induced higher yields.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:235The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Development of the reproductive tract of immature gilts
165-166Views:72Mechanisms of regulation of litter size in pigs are complex and depend on many factors, including genetic regulation and also physiological and anatomical development of the reproductive tract in gilts. Improvement of growth rate in present breed pigs raises the question as to whether, with the development of the reproductive tract, sexual maturity would also be attained.
The aim of the study was to assess the morphometric traits of reproductive tracts taken from gilts slaughtered at 100 kg body weight, i. e., just when they may become actively sexual mature.
This study was concluded on 80 prepubertal gilts of the Polish Landrace (PL) breed tested at the Pig Program Testing Station. The animals were kept in individual pens with control feeding and standard management. They were slaughtered after attaining 100 kg body weight. Immediately after slaughter, the reproductive tract was removed and carefully assessed. The morphometric estimation of the reproductive tract involved the
measurement of uterus weight with ligament, vagina-cervix length, uterine horns and oviducts length, ovaries weight, height and width. Uterus volume capacity was also determined, based on volumetric method of Kwaśnicki’s (1951) with own modification.
All pigs were divided into three groups in respect to age at slaughter: A – below 160 days (n=38), B – from 160 to 180 days (n=28) and C – above 180 days of age (n=14). The results were elaborated statistically computing the arithmetic means (x) for every traits and standard deviations (s). One-way analysis of variance ANOVA was performed. The significance of differences between age groups was estimated using Duncan’s test.
Calculations were performed with STATISTICA 8PL Software.
Obtained results are presented in the tables below. The most pronounced differences in the development of the reproductive tract are dependent on the age of gilts concerning only the uterus weight (P≤0.01) and uterus vagina-cervix length (P≤0.05). Gilts at age 160-180 days attained the full stage of reproductive tract development. Differences between the compared age groups of gilts dealing with the other morphometric traits
and ovary characteristics were statistically not significant. -
Researches regarding the influence of the some technological elements on water use efficiency in maize from Crisurilor Plain
5-9Views:153Plain and the influence of the hybrid, plant density, crop rotation, nutrient supply, weeds and irrigation on water use efficiency were studied.
Choosing of the hybrid with the best water use efficiency is very important because a hybrid from 500–600 FAO group (Fundulea 376) in unirrigated conditions and a hybrid from FAO group over 600 (Fundulea 365) obtained the biggest water use efficiency; the hybrid Fundulea 365 obtained the highest irrigation water use efficiency, 20.1 kg yield gain 1 mm-1 irrigation water.
One of the most known hybrid in the area is Turda super and the highest water use efficiency was obtained using the plant density of 55 000 plants/ha in unirrigated variant and 70 000 plants/ha in irrigated variant. The highest irrigation water use efficiency, 20.7 kg yield gain 1 mm-1, was obtained at 70 000 plants ha-1.
In maize monoculture was obtained the lowest values of the water use efficiency in unirrigated and irrigated variant: in the wheat-maize crop rotation the values were higher than in maize monoculture and in the wheat-maize-soybean were registered the highest values. The same situation was registered regarding the irrigation water use efficiency.
Farm manure (30 t ha-1) and especially manure (30 t ha-1) +chemical fertilizers (N90P45) determined a higher values of the water use efficiency in comparison with the control. In the variant with organic + mineral fertilization was registered the higher value (19.4 kg yield gain mm-1) of the irrigation water use efficiency.
Water use efficiency was much lower in the variant with weeds in comparison with the variant without the weeds; the differences were of 69% in unirrigated variant and of 64% in irrigated variant, very significant statistically. Irrigation water use efficiency from variant with weeds was lower than the value registered in the variant without weeds; the difference (68%) was very significant statistically.
In average in period 1976–2012, the irrigation determined the increasing in water use efficiency with 22%, 19.4 kg mm-1 vs. 15.8 kg mm-1, but not in all the years caused the irrigation increasing in water use efficiency in comparison with unirrigated maize.
The results research emphasized the need of the optimization for technology elements studied and a better water use efficiency will be obtained.
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The impact of grain maize and sunflower as previous crops on the yield and ear number of winter wheat in a long-term experiment
75-79Views:136We have analyzed the effect of grain maize and sunflower forecrop on three different winter wheat genotype (GK Csillag, Mv Csárdás, Mv Toldi) regarding to the spike number and yield in different fertilization levels in the 2012/2013 cropyear. According to our data after sunflower forecrop we reached higher spike number and yield, which were influenced by the differences of the varieties. We found significant differences between the varieties in the control treatment. According to our research, GK Csillag has reached the highest yield in the examined fertilization levels and forecrop models too.
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The effect of compost made of sheep manure on the first cut of a semi-natural grassland
25-29Views:228In an experiment, two types of compost were tested on natural grassland in order to improve the productivity of a natural sward on solonetz soil. Both composts were made of on-farm produced sheep manure, but the second one was enriched in phosphorus. These fertilizers/composts are officially authorized and can be applied in organic farming. Zero application and three rates of fertilizer were tested (10 t ha-1, 20 t ha-1, 30 t ha-1) on 30 m2 experimental plots wit four replications. Dry matter, crude protein and net energy content for maintenance were measured and determined and their yields per unit area were calculated. We found that all the treated plots produced significantly higher yield than the control ones, and in some cases the yields were almost three times higher. However, the optimal compost dose varied, depending on the examined parameter. Based on the evaluation of the experiment results along with rainfall data, it was concluded that, with higher precipitation, the positive effect of the compost application was bigger. The difference between the natural and enriched compost is remarkable, but it has to be mentioned that even the natural compost showed good results; therefore, it is also effective in improving the fertility of grassland.