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  • Changes in the Sulphur Content of Winter Wheat in a Field Experiment
    85-88
    Views:
    79

    The use of superphosphate as P-containing fertiliser decreased in the last years in many countries in accordance with strict air pollution laws, and the S-deposition decreased from the atmosphere to the soil as well.
    Winter wheat is the one of the S-demanding plants. Recently, the gradually increasing S absence endanger the formation of required average yield of winter wheat, and has bad effect on its quality.
    We examined the effect of treatments on the sulphur-, nitrogen content and the N/S ratio of winter wheat in the whole upperground plant and in the grain and straw at harvest in a arable land sulphur fertilization experiment on brown forest soil (Agricultural Company of Felsőzsolca).
    We analysed the samples from spring to harvesting, in the critical phenophases. In this study we discuss only the values from the stooling and stalking and the results of analysis of grain and straw in the harvest.
    We experienced that the concentration of sulphur in the whole upperground parts of winter wheat showed increase to the end of vegetation independently of fertilization. The N/S ratio was between 8% and 12% in the beginning of the growth period in the whole upperground plant, while the ratio in the grain at harvest was between 13 and 14%. When we examined the whole upperground plant, stalk and leaf at stalking, we got the highest sulphur content in the leaf. Mostly the middle level sulphur fertilization dose (4 l/ha) increased the sulphur accumulation in the green plant. At total maturing, the greatest part of accumulated sulphur is in the grain, but then the effect of fertilization is less glaring.

  • Molybdenum - accumulation dynamics of cereals on calcareous chernozem soil
    81-85
    Views:
    123

    This work is about the molybdenum-accumulation of cereals analyzing soil and plant samples from a field experiment set in
    Nagyhörcsök by Kádár et al. in 1991.
    In this long-term field experiment different levels of soil contamination conditions are simulated. Soil and plant samples were collected
    from the experiment station to study the behaviour of molybdenum.
    In this report results of maize, winter wheat, winter barley and soil analysis are presented. The conclusions are as follows:
    – Analysing soil samples from 1991 we have found that roughly half of the molybdenum dose applied is in the form of NH4-acetate+EDTA soluble
    – Comparing element content of grain and leaf samples we have experienced that molybdenum accumulation is more considerable in the  vegetative plant parts
    – Winter wheat accumulated less molybdenum then maize in its vegetative parts. Comparing molybdenum content of winter wheat to winter barley we found that the concentration of the element in wheat was lower by half than in the winter barley. It seemed that molybdenum accumulated to the least degree in winter wheat.

  • Characterisation of basic water balance parameters of Debrecen
    35-40
    Views:
    201

    This work aims to develop a hydrological modelling tool to help managers make the right decisions for Debrecen, in the face of water scarcity and the increase in agricultural and domestic needs over time. The methodology was based on the creation of a climatic database, at monthly time steps, from 2016 to 2019, and cartographic (land use, digital elevation model, and hydrological network). As a next step, the watershed was delimitated into sub-basins to determine the shape and the physiographic characteristics of sub-watersheds. Finally, a hydrological study was prepared by calculating the time of concentration to build a database of water resources in the study area. This water resource will be used as an input parameter for urban farming.

  • A tárolási feltételek hatása a kukorica Fusarium fertőzöttségére és toxin szennyezettségére
    28-32
    Views:
    222

    Corn samples harvested in 1997, 1998 and 1999 from different soil types were stored at different conditions (temperature, moisture content, state of kernels) for six months. The Fusarium contamination was examined by plate dilution method and the amount of mycotoxins (F-2, T-2, HT-2, DON, DAS) were determined applying HPLC method immediately after harvesting and in the third and sixth month of storage. The aim of our study was to find correlation between the ecological factors, storage conditions and the examined parameters mentioned above, as well as to prove them statistically. According to the examinations carried out after harvesting we could state that the soil type had no effect on the parameters. Analysing the effect of the years we found considerable differences. The Fusarium infection of corn samples in 1998, while the toxin contamination in 1999 was the highest. The results of storage experiment show that year (number of microscopic fungi, F-2, T-2, HT-2, DAS and total toxin content) and moisture content of kernels (F-2, T-2, and total toxin content) have a significant effect on the examined parameters. We could prove the effect of temperature on the T-2 content (samples with natural moisture content) and DON content (samples with 14% moisture content). Higher values were found at higher temperature storage. The ratio of damaged kernels influenced the DON concentration in the non- wetted samples and the number of microscopic fungi in the wetted samples.

  • Response of maize seedlings to the latent zinc deficiency under different Fe/Zn rati
    125-128
    Views:
    93

    Zinc (Zn) deficiency is a critical nutritional problem for plants and peoples all over the world. Almost half of the world’s cereal crops are deficient in Zn, leading to poor crop yield. In this study, the effect of different Fe/Zn ratio on some physiological parameters of maize seedlings were investigated on the dry matter of shoots and roots and their ratio, SPAD index and the total length of shoots. The relative chlorophyll contents significantly decreased under increasing Fe given to the Zn deficient. The results showed that the different Fe/Zn supplies decreased the total length of shoots - ranging from 9% to 65% - by latent Zn deficiency. Corresponding to several scientific findings, it was observed that the non-optimal Fe/Zn ratio of tissues take part in the evolution of latent Zn deficiency in the case of high Fe concentration.

  • Application of the Collision Cell (CCT) in the ICP-MS Analytical System
    120-125
    Views:
    164

    Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.

  • Testing laboratory parameters of compost tea
    31-36
    Views:
    430

    During the industrial production of broiler chicken, a large amount of manure is produced, of which easily contained nitrogen content (without pre-treatment) is released into the atmosphere as an air pollutant. In our experiments, we aimed to prepare compost tea, also known as water extract of compost, from pre-treated poultry manure in order to create a product can be utilized as liquid nutrient supply. The poultry manure source was the Baromfi-Coop Ltd. located in Nyírjákó, Hungary, where it was treated by composting. As a result of this pre-treatment of the poultry manure, its nutrient parameters improve and nitrogen is present in a form that is better utilized for plants. Furthermore, this product is suitable for further utilization and also can be the base material for a brand-new product. For this reason the effects of compost/water ratio, incubation time, low oxygen level, and extraction time on the parameters of the resulted product were studied in the frame of developing new soil-life enhancing microbial product, so-called compost tea.

    Chemical parameters of the compost used as base material strongly determined the properties of the resulted compost tea, especially the ratio of the various nitrogen forms, their concentration and the salt content. It was found that adding water at a higher rate that means 1/40 and 1/50 mixing ratios results in more cost-effective production. In the experiment the compost tea were held under oxygen-poor conditions, therefore pH of the extractions decreased, which influenced the quality and quantity of their nutrient content.

  • The recent state of cryopreservation techniques for ex-situ gene conservation and breeding purposes in small ruminants: A review
    81-87
    Views:
    290

    The viewpoint of the recent cryopreservation techniques (CT) suggests the use of a reduced volume of cryopreservation solution, high concentration of cryoprotectants and ultra-rapid cooling and warming rates help to reduce cryo-injury and maximize the viability of the preserved animal genetic resources (AnGR). The CT had now become widely accepted as one of the best methods of choice for the ex-situ conservation of AnGR due to its high success rate recorded and no-invasive nature as compared to the conventional slow rate freezing (CSRF). Rapid advances and wide acceptability of the use of assisted reproductive technologies (ART’s) particularly artificial insemination (AI) in animal breeding had resulted in a greater loss of a large number of good quality genes in virtually almost all the native breeds of animals across the globe. Small ruminant (SR) animals are not an exception in such present predicaments situation of erosion and dilution of the valuable AnGR among the native breeds. As a result of this, 148 and 16 breeds of sheep and goats respectively have already become extinct in Europe and the Caucasus. In view of the aforementioned situation, the present review aimed at exploring some of the current states of development, roles played and potentials of CT in the conservation of SR genes and genome for the immediate and future breeding purposes for sustainable development. It basically covers; animal genetic resource, the need to conserve AnGR, tools for ex situ in vitro conservation of AnGR and recent developments in breeding and cryopreservation of SR AnGR.

    Cryopreservation is playing a pivotal role in ex-situ gene conservation of AnGR. Decline in genetic diversity among SR breed population was high in Europe and the Caucasus. There is therefore, need for improvent on current stringent measures on conservation of AnGR in this region of the world.

  • Pre-treatment of wastes containing keratin in industrial conditions
    59-64
    Views:
    123

    The aim of the research was a development of a featherdegradation technology in industrial circumstances. During the experiments we determined the parameters of the critical environmental and technological limitation factors. Because of the high keratin-content the degradation (and composting) of the feather is difficult. With the developed technology huge mass of feather can be used fast and easily in biogas production and in the adjustment of the optimal element ratio of biomass. The industrial experiments were implemented in a 6m3 heatable double-walled
    tank with stirring-shovels and aeration-system. The degradation process was followed with extinction measurements.
    According to the experiments that were done the best results were given at the case which was heat-treated at 70°C, was injected with 1% bacteria concentration, and where 1:3 feather:water ratio was set if we consider the effectiveness of degradation and the hydraulical retention time.

  • Analysis of aerobic biological waste treatment methods especially in the case of composting
    33-37
    Views:
    146

    In recent years the regulations of the EU unambiguously determine that the biodegradable wastes should be used in agriculture. The characteristics of the organic wastes in most cases make the direct utilization impossible, they need pre-treatment before use. One treatment solution of these wastes is composting. During composting the organic wastes lose their hazardous characteristics and we gain a final product, the compost, which can be used in agriculture as organic fertilizer. The main conditions of effective composting are the follow and understand of the degradation process. During our research we examined different measuring methods (gas concentration and reflectance measurements, temperature mapping) that makes a cost and time effective possibility to directly analyze the degradation.

  • The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
    135-140
    Views:
    162

    Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.

     

  • Spatially Discrete GIS Analysis of Sampling Points Based on Yield and Quality Analysis of Sugar Beet (Beta vulgaris L.)
    32-37
    Views:
    89

    Fulfilment of the increasing quality requirements of sugar beet production can be analysed with sampling of plants and soil at the cultivated area. Analyses of the spatial characteristics of samples require exact geodetic positioning. This is applied in practice using GPS in precision agriculture. The examinations were made in a sample area located in north-western Hungary with sugar beet test plant. According to the traditional sample taking procedure N=60 samples were taken in regular 20 x 20 m grid, where besides the plant micro and macro elements, the sugar industrial quality parameters (Equations 1-2) and the agro-chemical parameters of soils were analysed. Till now, to gain values of mean, weighted mean and standard variance values, geometric analogues used in geography were adapted, which correspond to the mean centre (Equation 3), the spatially weighted mean centre (Equation 4), the standard distance (Equation 5), and the standard distance circle values. Robust spatial statistical values provide abstractions, which can be visually estimated immediately, and applied to analyse several parameters in parallel or in time series (Figure 1). This interpretation technique considers the spatial position of each point to another individually (distance and direction), and the value of the plant and soil parameters. Mapping the sample area in GIS environment, the coordinates of the spatially weighted mean centre values of the measured plant and soil parameters correlated to the mean centre values showed a northwest direction. Exceptions were the total salt and calcium-carbonate contents, and the molybdenum concentration of the soil samples (Table 1). As a new visual analysis, the spatially weighted mean centre values of the parameters as eigenvectors were projected to the mean centre values as origin. To characterize the production yield, the raw and digested sugar contents of the sample area, the absolute rotation angles of the generated vectors were determined, which indicate numerically the inhomogenity of the area (Figure 2). The generated spatial analogues are applicable to characterise visually and quantitatively the spatial positions of sampling points and the measured parameters in a quick way. However, their disadvantage is that they do not provide information on the tightness and direction of the spatial correlation similarly to the original statistical parameters.

  • Az NPK-trágyázás hatása a kukorica tápelemfelvételének dinamikájára, öntözött és nem öntözött viszonyok között
    23-27
    Views:
    168

    The effect of NPK-fertilization on the dynamics of nutrient uptake of maize (Zea mays L., cv. Clarica) was examined on chernozem soil under irrigated and non-irrigated conditions in a field experiment.
    The following results were made:
    • the element concentrations in the plant decreased over time,
    • there is no difference between the dynamics of nutrients on irrigated and non-irrigated sites because rainfall was satisfactory for plants in vegetation period,
    • the N doses not only significantly increase the nitrogen content in maize, but also have a noticable effect on Ca and Mg concentrations,
    • because of the acidifying effect of N-fertilizers, increasing the amount of N-fertilizer increased the Mn, Zn, Cu content of the plants,
    • the P doses have a significant effect on the maize P and N content and the Zn concentration of the plant via P-Zn antagonism in the soil,
    • as the high K doses treatments alter the ion ratios in the soil, the Ca, Mg content of the plant decreased.

  • Changes of Quality of Winter Wheat During Maturing
    105-112
    Views:
    104

    We analysed the flour of two winter wheat varieties to determine the effect of maturity state and grain position in the ear on the element composition of flour. Samples were taken from the 14th to the 42th day of anthesis. Ear samples were cut into three (basal, central and apical) parts, the trashed grains were milled, and the element compositions of the flours were determined.
    We found that the grain position within the ear has an effect on flour element content. Flours of apical grains had lower nitrogen, phosphorus and sulphur contents than flours of central and basal grains. The nutrients can be divided into two parts, based on the formation of element content during maturation. Nitrogen, sulphur and copper contents changed continuously during maturity, while the P, K, Ca, Mg, Mn, Sr and Zn contents decreased until a specific value and became nearly constant in the second half of maturity. We found that – although the nitrogen concentration of flours changed continuously – the amount of nitrogen in the flours reached a constant value for the second half of maturity.

  • Use of essential trace elements enriched Artemia in the larval rearing of barramundi (Lates calcarifer)
    11-16
    Views:
    153

    Artemia is a vital nutriment in the rearing of barramundi (Lates carcarifer), however it’s mineral trace element content does not cover the requirements of the larvae. In our experiment the assumption was wether the cobalt, zinc and manganese concentration of Artemia could be increased during a 24 hours of enrichment period, as well as we wanted to investigate the resulted interactions between the elements. For this purpose 50, 100 and 1000 mg l-1 cobalt-chloride treatements (Co50, Co100, Co1000) were applied while in the case of 50 and 100 mg l-1 treatments the enrichment was also complemented with zink-sulphate and manganese-chloride in the same concentrations. According to the results the zooplankton were able to accumulate cobalt in higher amount, and yet zinc and manganese had no significant effect on each other, a strong synergistic effect occurred between cobalt and manganese. After the enrichment period the impact of essential trace element supplemented live feed on the survival and growth of barramundi larvae was also studied. The experiment was carried out by using 15 days-old larvae applying a total of 9 treatments for two weeks (Control, Co50, Co100, Mn50, Mn100, CoZn50, CoZn100, CoMn50, CoMn100), in duplicates. Considering the per cent of survivals, no significant difference was observed between the treatements (P>0.05). In the case of CoMn50 and CoMn100, the loss statistically increased (P<0.05) compared to the Control (80.5±4.95%) group. Our results show, that the Mn100 and CoMn100 treatements resulted in  ignificantly higher (P<0.05 ) in divid ual bod y w eight s, how ever taki ng the survival also into consideration the Mn100 treatements proved to be the most
    effective.

  • The Effect of Grazing Intensities on Magnesium Contents
    8-13
    Views:
    206

    Research was carried out on two areas of grassland in Hortobágy National Park, Hungary. Two herds of Hungarian Grey Cattle were kept in free range grazing and the effects of grazing pressure on the magnesium content of soil and ryegrass (Lolium perenne L) were determined.
    Changes of plant available and total soil magnesium content under different grazing intensities did not show any evident tendency on the investigated grasslands. Different amounts of cattle faeces, urine and trampling had no effect on the magnesium concentration of ryegrass. We conclude that the magnesium content of ryegrass on both grassland sites as moderate grazing and overgrazing matches the requirements of cattle. Symptoms of magnesium deficiency of cattle will likely not appear.

  • Wine adulteration and its detection based on the rate and the concentration of free amino acids
    139-151
    Views:
    334

    Following the definition of wine adulteration, the authors briefly review its history from ancient times to present day and describe the methods that are applied for adulteration of wine from the historical point of view. More space is devoted to the Hungarian wine adulteration and to the detailed reports of the early methods, which attempted to discover fake wine. It describes in detail the current situation of wine adulteration and the fight against counterfeiting. The second half of the review article presents some examples of the analytical chemistry techniques with which fake wines can be detected. In doing so, priority is given to the discussion of high performance liquid chromatography and gas chromatography applications. The end of this paper describes the free amino acid content of wines, and the possibilities of using the results for detection of wine adulteration.

  • Preliminary test: Evaluation and selection of tomato (Lycopersicon esculentum Mill.) varieties resistant to drought and powdery mildew
    33-37
    Views:
    132

    Powdery mildew (Leveillula taurica) and (Oidium neolycopersici) are two harmful fungi that invade the tomato (Lycopersicon esculentum Mill.) plant and grow in dry conditions. Under the influence of polyethylene glycol 6000 at a concentration of 12%, the total seedlings fresh weight, total seedlings dry weight, seedling growth rate of one seedling, seedling length, and tissue water content percentage for three tomato varieties were assessed. Despite the superiority of the (Mobil) tomato variety in terms of numerical values, the results revealed no significant differences between varieties. As a result, (Mobil) has greater vigor under environmental drought stress of lower osmotic stress than other tomato varieties. More work is required to evaluate the research selection of varieties resistant to biotic stresses in dry areas, such as powdery mildew disease.

  • Qualitative detection of genetically modified organisms in plant samples
    309-313
    Views:
    125

    We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.

  • The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)
    39-44
    Views:
    153

    Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.

  • Formation of Food Clusters in Europe
    55-59
    Views:
    89

    In the dictionary of foreign words and phrases we can find the word “cluster” with the following sense: aggregation, group and in a wider meaning: association, integration.
    By explaining the geographical concentration of economical and innovation processes in the food processing sector, clusters, and especially
    business clusters, play an important role. This essay deals exclusively with business clusters, so the term cluster is used also for business cluster.
    I tried to collect the most relevant indicators determining the formation of clusters in the Food Processing Sectors and to analyze whether a
    correlation can be found among the analyzed indicators.

  • Effect of ethephon levels and amino acids on the growth characteristics of oat crop
    57-61
    Views:
    75

    A field experiment was conducted during the 2021-2022 season in an agricultural field in Basra Governorate. The aim was to study the effect of spraying ethephon and amino acids on the growth characteristics of oats (Avena sativa L.) Shifa cultivar. The experiment involved three levels of ethephon (0, 0.240, and 0.600 kg ha-1), represented by the symbols E0, E1, and E2 respectively, and two spray levels of amino acids (0 and 5 ml L-1), represented by the symbols A0 and A1. A factorial experiment was applied using the R.C.B.D (randomized complete block design) in a split plots arrangement with three replications. The results revealed significant differences in the levels of ethephon for most of the studied characteristics. The level of 0.240 kg ha-1 (E1) was superior in terms of chlorophyll content, crop growth rate, and the number of tillers, with increases of 39.07%, 39.26%, and 16.36%, respectively, compared to the control treatment (E0). Regarding the amino acids treatments, spraying at a concentration of 5 ml L-1 (A1) demonstrated significant superiority, resulting in the highest plant height, flag leaf area, chlorophyll content, and crop growth rate. This study concludes that amino acids play a crucial role in plant growth, and the combination of 5 ml L-1 amino acids with 0.240 kg ha-1 of ethephon significantly increased chlorophyll content and crop growth rate.

  • Storage of ram semen in gelatin supplemented extender
    7-10
    Views:
    126

    The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.
    We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p>0.05).

  • The use of essential trace elements in the juvenile rearing of barramundi (Lates calcarifer
    33-38
    Views:
    200

    Barramundi (Lates calcarifer L.) is a predatory fish species native in Southeast Asia and Australia. Based on the geothermal potentiality of Hungary the warm-water fish can be reared successfully. Zooplankton in the wild contains minerals in a higher concentration than the usually fed newly hatched Artemia nauplii, therefore essential trace elements, such as cobalt, zinc and manganese play an important role in the larviculture of barramundi. Cobalt is vital in trace amount for many living functions of vertebrates, however, lower number of papers are available considering the nutritional aspects. Nevertheless. improved growth performance was observed in cases of some fish species when diet was supplemented with CoCl2. Zinc and manganese are also vital for optimal growth and accordingly are investigated and applied diet supplements in aquaculture.

    The main aim of the recent study is to investigate the effects of cobalt, manganese and zinc on the growth performance and homogeneity of fish when a commercially available dry diet is supplemented with trace elements individually and in combined treatments. A total of 6 treatments were set in a randomized blocked design where the concentrations of the applied elements were 50 mg kg-1 for CoCl2, for ZnSO4 and for MnCl2 individually, as well as for CoCl2 along with ZnSO4 and for CoCl2 along with MnCl2 in combination. Although the production parameters of larval barramundi were positively affected by the addition of trace elements when the retention of minerals occurred through nourishment living organisms, statistically no differences were found between the treatments considering the growth performance of barramundi juveniles either when dry feed was supplemented with cobalt, manganese or zinc (p>0.05). While the use of cobalt and manganese in combined treatments produced a less uniform larvae in size and as a consequence of increased heterogeneity, survival was significantly reduced by the cannibalism, the sizes distribution of barramundi juveniles wasn’t affected by the dry diet supplementation of these elements.

  • Regional restructuring, reindustrialization and development of the higher education
    173-176
    Views:
    93

    The development of the new economy in Hungary involves a complexity of economic and social processes. The sectoral structures and territorial location of the industy and the higher education show significant inequalities. Capital city concentration, low level of research orientation and insufficient linkages with industries are the most important barriers of the development of higher education and reindustrialization. The regional and cohesion policies need strong relations between economic restructuring and higher education development. The paper focuses on demonstrating the regional differences in these fields in southern and eastern Hungarian regions.