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Some population genetics parameters of the present Hungarian Hucul Horse population
15-22Views:150We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.
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The egg production of hungarian speckled hen and speckled transylvanian naked neck hen
11-15Views:75Our Faculty has been dealing with the cross-breeding of Hungarian speckled hen to maintain the breed since 1977. We keep two breeds of the Hungarian speckled hen, the bare-neck variant and the feathered-neck type on the pilot farm. Because of the spread of intensive poultry keeping the population of these breeds has become endangered. Beside the gene preservation, we endeavour to find the best way for the production-purpose
utilisation of the speckled hen stock. We examined the egg production of these breeds. -
Changes in the relationship between Hungarian Grey cows (Bos primigenius taurus hungaricus) and their calves in the period from calving to four months of age
85-90Views:110The ability of cows to care for their offsprings is a very important trait concerning beef cattle. Maternal instincts are highly influenced by breed, specific cow, and social and rearing environment. In 2020, at the Szamárháti farm of the Tiszatáj Public Foundation, we selected 15 dam-calf pairs and studied them for four months from the birth of the calves. We grouped calf-rearing behaviors according to strength and analyzed the related changes during the program. At the beginning of the relationship, "standing close to each other" (53%) and "suckling" (24%) characterized the pairs. After the first month, "standing or lying separated" grew by 69%, and "vocalization" by both animals fell by 66%. Even though the daily frequency of suckling decreased by 34% as the calves grew older, it remained a typical element of the relationship. Over time, the bond between the animals gradually weakened. This natural process of separation takes a month to complete.
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The effect of breed and stage of lactation on the microbiological status of raw milk
37-45Views:296The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.
There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.
Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.
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The results of the investigation of the β-casein gene polymorphism in sheep breed – Review
75-80Views:169β-casein is the most abundant protein fraction in sheep milk, and has at least six different alleles (A, B, C, G, X, Y). The alleles of the β-casein gene may influence on the quality and quantity of milk. Knowing the gene polymorphism has an important role in the process of milk production. The properties of milk could be positively influenced by themolecular genetic methods.
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Perspectives of the livestock sector in the Philippines: A review
175-188Views:1999The Philippine livestock industry is a vital component in the country's agricultural sector. It contributes around 18.23 percent of the gross output value in agriculture and provides livelihood to many people living in rural areas, signifying its importance with regard to the country’s economy. The constantly increasing demand for animal protein associated with the increase of the world’s population led to the intensification of livestock production, realized through the various initiatives implemented by the government, such as the import of high producing purebreds of various species. This strategy greatly influenced the country's animal genetic resource's diversity and increased food animals' population; however, it impacted the native breeds of food animals. Understanding the characteristics, performance and value of native and exotic breeds is essential to prioritize their livestock industry existence. Thus, this paper aims to characterize various native and exotic breeds of livestock in the Philippines and assess the current conservation of native animals.
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Challenges in the mangalitsa sector – present and future
147-153Views:120The Hungarian mangalitza pig sector has experienced numerous structural changes in the past few decades. The increased demand in the foreign markets for the mangalitza pork, changes in the domestic consumer perceptions have increase the number of sows and breeders. After the European Union’s accession started a significant growth in the sector, which was due to the target programme from the year of 2005 for the keeping of animals representing high genetic value, as the breed is indigenous. From 2008 continuous decline can be observed. The main reason for the decrease was the drastic growth of feed costs because of the drought damage in 2007, and the global economic crisis. Since 2010, a further period of supports has been in place, it extands the willingness to keep mangalitza pigs and the number of sows continuously increasing. On the basis of the average farm size (58 sows/farm) in 2011 it can be stated, that after our EU’s accession the medium-sized farms became stronger. To evaluate the geographic concentration of the mangalitza livestock it was analysed the data of sow number among the years of 2000 and 2011. The geographic concentration of mangalitza stock has a medium value over each year under the survey period, except the years of 2004–2007, when the values of Herfindahl-Hirschman index (HHI) have low degrees. Due to the subvention period of indigenous breeds it was established more mangalitza farms in different parts of Hungary. Despite of the growth since 2000, numerous problems are in the segment. The aim of the study is to find out the sectoral problems, which are completed with the objectives tree and draw up the main activities to solve the problems. After estimating the SWOT-matrix according to the methodology of the strategic analysis and the discussions with the secretary of National Association of Mangalitza Breeders is prepared the sectoral problem- and objectives tree. The core problem of the mangalitza sector is that the origin of mangalitza products are not certified in the domestic markets. The reason of it is that the breeders don’t request the certificates of fattening pigs, so it can appear not real mangalitza product on the markets as mangalitza. To solve the problems it is drawn up different suggestions in the objectives tree. This analysis is an useful tool for the decision makers in the mangalitza sector to evolve strategic plans on behalf of the efficient cooperations among chain actors.
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New Possibilities of Brown Bullhead (Ictalurus nebulosus) Farming
55-57Views:86The brown bullhead (Ictalurus nebulosus) has been settled in Europe at the end of Century XIX from North America. In Hungary it has been brought in 1902. The naturalization of this species was successful because the brown bullhead found adequate conditions for its life and reproduction in Hungary. But it was unsuccessful because lost its excellent growth.
In inland water habitats-and that’s why also fishponds-extraordinarily multiplied, but because of its slight growth this species is undesirable for the native farmers. The brown bullhead is concurrent for domestic fish species, on the other hand its weight not more than 150-200 g. Occasionally it was exterminated, but multiplied again and again.
In 1999 we placed out black bullhead offspring in a pool of a new intensive fish farm, in order to breed at least 300-350 g weight for the customers. The accidental trial was surprisingly successful. Consequently, the black bullhead prefers the artificial food, and produces excellent growth parameters and specific feed-live weight conversation ratio.
On the 20th of September 1999 there was placed 23 300 pieces of brown bullhead offspring, with 28 g average weight and 625 kg gross weight. When we sold them on the 22nd of February 2000, the gross weight was 3387 and the live weight gain was 2735 kg. The gross weight of the feed was 3041,8 kg, and the feed-live weight conversation ratio was 1,11 kg/kg.
Because of this successful trial the managers of the farm wanted to start systematic experiments to work out the details of the intensive farming of brown bullhead. We undertook to help to find the right place of the species in the Hungarian fish processing because of the increasing native and foreign demand. -
Coagulase-negative staphylococci in ewe udder surface and raw milk samples
221-225Views:132Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.
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Examination of extensographical parameters of winter wheat (Triticum aestivum) flour
109-115Views:107In the trade of the European Union principally the analysis of alveographical and extensographical parameters mean the acceptance system.
In the present study we analysed the extensographical parameters of 10 winter wheat varieties breed by the Cereal Research Non Profit Company with Brabender extensigraph and we made a comparison, correlation among the results. The examinations with Brabender the following among: show the GK Kalász and the GK Élet varieties show high resistance of extension and the GK Petur variety produces high extensibility. The GK Garaboly has shown low extensibility and energy. The other parameters had different values in the examined three years. The measurings with SMS2 Texture Analyser show middle positive correlation between extensibility and subarea. There are weak correlation between at the significantial level in the 90, 135 resistence of extension by Brabender and by SMS2 Texture Analyser and in the 45 extensibility by Brabender and by SMS2 Texture Analyser of relaxed dough. There is no correlation among other parameters. -
Evaluation of the relationship between main type traits and longevity in Hungarian Simmental cows
205-209Views:108The Hungarian Simmental cattle is a dual-purpose breed, having good milk and meat production characteristics. Simmental cows have some other important traits, for example, a longer productive life. The longevity or productive life is the time period between first calving and culling. The conformation contributes to productive life, reproduction, and milk production.
The aims of this study were to analyze the longevity of Hungarian Simmental dual-purpose cows, to evaluate the effects of the size of the herd, age at first calving, main type traits (frame, musculature, feet and legs, mammary system), and combination of main type traits (frame and musculature, feet and legs and mammary system).
Animal, age at first calving, herd*calving, musculature, the mammary system as well as the combination of mammary system and feet and legs were significant effects on longevity. The highest risk ratio was observed for cows first calved after 31 months. The risk of culling increased with increasing scores of musculature and decreasing scores of the mammary system. The highest risk ratio was estimated in category 11 (lower scores of mammary system with lower scores of feet and legs). In this case, the risk ratio was 36% higher than the reference group.
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Relationships of Fruit Production and Regional Development in the Northern Great Plain Region
181-187Views:87The role of retaining population in agriculture is stronger and more significant in the long run in the North Great Plain Region compared to other regions. The region has a significant processing industry along with a good basis for producing raw materials, developed food processing capicity and high quality agricultural products typical of the region. The GDP in agriculture, forestry and game management is somewhat higher than the national average.
Variety is of cardinal importance when establishing the quality of horticultural products and determining the product value. The Hungarian breeding results of apple, quince, apricot, cherry, raspberry, red and black currant are promising.
The regulation system of EU the vegetable and fruit market is based on Retail Cooperatives Producers. With the establishment of national vegetable- and fruit production and retail organizations, the market regulation, production and quality development issues of the sector can be handled and solved. Reaching EU standards in fruit production can only be achieved with up-to-date plantation systems and breed selection. A key issue in development is establishing the required financial resources for investments and updating production. In order to reach these standards, significant state subsidies and good credit conditions are needed. By solving these tasks, the sector is expected to become self-financed without government help.
The pursuit of safer production, improving quality and increasing yields require the establishment of up-to-date irrigation systems. The improvement of family farms, motivating land concentration is necessary for increasing average size of plants. Establishing the above mentioned conditions is important since the vegetable-fruit sector is of great significance in the employment of rurally based population, improving their living conditions and executing rural development programs. -
Genetic diversity study in Hungarian coldblooded horses
29-34Views:202Because of the feeding technology innovation, accelerated transport and communication facilities breeds of high performance breeds replaced local autochone breeds worldwide. These latter species however have an important role in gene conservation. Hungarian cold-blooded horse breeding stock are lacking pedigree, the actual founder breed mares are not known. For this reason, it is an major priority defining the genetic backround of the existing flock, for that breeding could operate with purposeful using of origin maternal founders. In the present study 195 cold-blooded Hungarian mares tail and mane sample were analized. Our analysis was carried out between 15531–15752 base pairs in mithocrondial DNA D-loop region, which reported a total of 222 base pairs. Fourtyone polymorphic sites were determined, which resulted in 39 haplotypes (h=39). The average pairwise differences were k=6.825. High haplotype and nucleotide diversity values were observed (Hd=0.968±0.003, π=0.026±0.003). Based on the previously defined variable positions of haplotypes defined by Jansen et al (2002), we groupped our haplotypes into haplogroups. 23 percent of the studied population (45 mares) belonged to haplogroup F1. Nearly 97% of the analyzed population was classified into one of eight haplogroups defined by Jansen.et al. (2002). This study gives genetic information nearly 25% of the Hungarian population. Another possibility would be patterning more mares or involving more genetic marker in the study which will assuming the possibility of a more comprehensive analysis.
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Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:91The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. -
Factors affecting the efficiency of superovulation and embryo transfer. Season and hormonal background
73-76Views:208Success of the embryo transfer programs is affected by many factors (breed, type, season, treatment). We assume, that periferical blood level of some metabolic hormones (IGF-1, thyroid hormones, leptin, insulin) affects the success of superovulation and ET. According to our results, there is a
significant difference between the in and out of season periferial blood level of IGF-1 and insulin. Furthermore, decrease in the periferic blood levels of IGF-1 and insulin is less drastic in the pregnant recipients. -
Agricultural land use and food safety
55-60Views:139The world’s food production needs to be doubled in order to cover the need of the population by 2050 even if it exceeds 9 billion. The output of agriculture is expected to increase by 1.7% every year until 2020 (OECD, FAO 2011) which is a major decrease in comparison with the average 2.6% increase of the previous decade. At the same time, the meat, dairy, sugar and vegetable oil consumption is likely to increase by 2020 to a higher extent than so far. Due to the increasing food prices, the amount of starving people will increase and food consumption will decrease – especially in developing countries – as people will be able to buy less and only cheaper food products. Also, obesity may become a more severe problem and the inequality of the population’s health status could increase.
One of the most important elements of adapting to global climate change is food safety; therefore, it is especially important to breed new biological bases and to introduce production systems which contribute to adapting to changed circumstances.
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Changes of Longissimus Muscle Area and Rump Fat Thickness in Hungarian Simmental Fattening Bulls Measured Using Real-Time Ultrasound Equipment
11-15Views:80The aim of the authors was to evaluate changes in the longissimus muscle area (LMA) and rump fat thickness (P8) based on real-time ultrasound scanning in the Hungarian Simmental cattle breed. Ultrasonic measurements were carried out on the same 11 Hungarian Simmental fattening bulls by Falco 100 (Pie Medical) equipment (I.: age: 357±23.47 days, live weight: 475.55±51.40 kg; II.: age: 418±23.47 days, live weight: 555.10±54.11 kg) on two occasions. Animals were kept in small groups, on deep litter, and fed on silage and concentrate. LMA and P8 were measured on the scans, between the 12th and 13th ribs by manual outlining. Results for the investigated traits during the examinations were as follows: P8: I.: 0.373±0.154 cm, II.: 0.624±0.161 cm; LMA: I.: 65.72±5.89 cm2, II.: 71.74±8.94 cm2. During the fattening period, P8 increased significantly (I-II.: t=3.73, P<0.001). A significant positive correlation was calculated between results of measurements I. and II. in the case of LMA (r=0.71, P<0.05). Results imply the possibility of selling bulls with smaller LMA earlier, at lower body weight. Application of ultrasonic measurements in fattening technology could generate a more quality-related pricing system.
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The role of animal breeding with special regard to native pigs of food supply and rural development in Laos
149-154Views:243The present paper explored the influence of breeding native pigs on livelihood conditions and the contribution of native pigs to the rural development and rural poverty reduction in the rural areas of Lao PDR. Pig production plays an important role in meat supply for both urban and rural areas of Laos. It is clear that most of the pig products in the country come from smallholder pig farms, and more than 90 percent of those products are the native pigs mostly raised by farmers in remote areas. In general, livestock production distributed between 15–18 percent to GDP, while most of animal production still remains as the traditional methods. Rural development is always the first priority of the Laos government since its independence in 1975, however, the poverty rate in rural areas remained high at 23% in 2018. It might block the development goal of the government which will lead the country out of the least development status by 2020. The food security and malnutrition in the rural or mountainous areas are considered as the majority issue that both government and several international organizations have been thriving hard to overcome, which researchers showed that more than 45% of children under 5 years of age were stunted, and 28% of them were underweight. Inspired of more than 50 % of the households in the rural areas of Laos reported they consumed chicken and pork at least one day a week. While native pigs play an important role on meat supply, it also constituted around 9–14 % of annual income of the households in rural areas. Therefore, the increase the production of pigs and poultry is one option to promote the meat supply to households in the rural areas of Laos. This paper will be a pathway to guide and identify for the final decision to what experiment will be implemented on Lao native pig in Laos (2021–2023) to complete the comparative study on reproductive physiology and reproductive management methods of Hungarian and Lao Indigenous pig breed. Which found it still needs further afford to research and improve more about native pig performance for all areas of productive and quality management.
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Evaluation of deep-frozen ram semen from different sheep breeds with live/dead acrosome staining
26-28Views:77It was found that the Kovács – Foote staining is properly adopted to examine deep-frozen ram’s semen. Data are appropriate for comparison. Examination of one ram’s semen per breed is not enough for drawing any conclusions; therefore, I will continue this research.
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Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
61-64Views:147In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.
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The recent state of cryopreservation techniques for ex-situ gene conservation and breeding purposes in small ruminants: A review
81-87Views:269The viewpoint of the recent cryopreservation techniques (CT) suggests the use of a reduced volume of cryopreservation solution, high concentration of cryoprotectants and ultra-rapid cooling and warming rates help to reduce cryo-injury and maximize the viability of the preserved animal genetic resources (AnGR). The CT had now become widely accepted as one of the best methods of choice for the ex-situ conservation of AnGR due to its high success rate recorded and no-invasive nature as compared to the conventional slow rate freezing (CSRF). Rapid advances and wide acceptability of the use of assisted reproductive technologies (ART’s) particularly artificial insemination (AI) in animal breeding had resulted in a greater loss of a large number of good quality genes in virtually almost all the native breeds of animals across the globe. Small ruminant (SR) animals are not an exception in such present predicaments situation of erosion and dilution of the valuable AnGR among the native breeds. As a result of this, 148 and 16 breeds of sheep and goats respectively have already become extinct in Europe and the Caucasus. In view of the aforementioned situation, the present review aimed at exploring some of the current states of development, roles played and potentials of CT in the conservation of SR genes and genome for the immediate and future breeding purposes for sustainable development. It basically covers; animal genetic resource, the need to conserve AnGR, tools for ex situ in vitro conservation of AnGR and recent developments in breeding and cryopreservation of SR AnGR.
Cryopreservation is playing a pivotal role in ex-situ gene conservation of AnGR. Decline in genetic diversity among SR breed population was high in Europe and the Caucasus. There is therefore, need for improvent on current stringent measures on conservation of AnGR in this region of the world.