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Our research aimed to determine what temperature changes occur along with the spinal segments during seated exercise and in which direction these temperature values change due to a preventive exercise program. This thesis aims to develop a thermographic analysis procedure to monitor the loads generated in and along the spine of mainly e-athletes and to investigate the effect of a preventive exercise program designed by the research team on the temperature values generated by the load. During our research, we conducted a study with three university students over several months. After an acclimatization period, the research subjects performed e-sport activities in a standardized temperature room while their back temperatures were monitored. The resulting thermograms were analyzed using ImageJ free-use image analysis software. The RGB-type thermograms were converted to 8-bit color depth, and the pixel intensity of the ROI (Range of interest) areas was measured. The following ROI areas were isolated during measurements: neck (C-cervical), back (T-thoracic), lumbar (L-lumbar), and muscle groups to the left (S-sinistrum) and right (D-dextrum) of these areas. Results were obtained at five-minute intervals. Our results showed that the intervention did not produce apparent changes. In two out of three subjects, the cervical section was the hottest, followed by the thoracic and lumbar areas. In general, surfaces to the left of the spinal regions were warmer than surfaces to the right.