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Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation
30-38.Views:340Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.
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Effects of different levels of NaCl and CaCl2 on seed germination characteristics of Melissa officinalis L. and Ocimum basilicum L.
21-25.Views:295More than 15 million hectares of Iran’s land areas are suffering from salinity.We investigated the effects of two types of salt (NaCl, CaCl2) in different levels on the germination and initial growth of two medicinal and aromatic plants, Melissa officinalis L. and Ocimum basilicum L. The experiment was carried out in Qazvin Agriculture and Natural Resource Research Center based on a randomized complete block design, for 14 days in a germinator. Sweet basil germinated above 50% in each saline treatment (EC=10 dSm-1), in alkali treatment (CaCl2=18800 mg/L) and in sodic-saline treatment (Ec=10 dSm-1; SAR=5.6), however at the highest concentrations germination was slowed down. Lemon balm showed a higher sensitivity: except the lowest NaCl treatment, each other ones inhibited germination and its rate to some extent. The combined treatments of both salts increased the stress reactions. However, seedling growth was less effected. The results showed a stimulating effect of NaCl treatment in 2500 mg/L (EC<5 dSm-1) for both species concerning germination and seedling growth.
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In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants
15-20.Views:396Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.