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Detection of natural infection of Quercus spp. by the chestnut blight fungus (Cryphonectria parasitica) in Hungary
54-56.Views:224The chestnut blight fungus Cryphonectria parasitica (Murrill) Barr [syn.: Endothia parasitica (Murr) Anderson] caused almost total destruction of the American chestnut (Castanea dentata) and widely spread on European chestnut (Castanea saliva) in many European countries. In Hungary, because this fungus threatens most of the Hungarian chestnut stands, great efforts have been made to delay its spread. Biological control with Hungarian hypovirulent strains of the pathogen seems to be an effective method for saving the affected chestnut trees. Until 1998 the fungus was detected on Castanea saliva only, then on some trees of young Quercus petrea in mixed chestnut forests, which also showed the typical symptoms of blight (Kőszeg and Zengővárkony). Although blight symptoms are not so serious in Quercus spp. than in Castanea spp., it seems that C. parasitica threatens the young Quercus spp. in Hungary, mainly in heavily infected chestnut forests. This is the first report of C. parasitica cankers on oak in Hungary.
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Study on the Penicillin-content of botrytized wines and noble rotted berries in Tokaj-region
91-94.Views:231Favourable parameters are offered to the formation of noble rot caused by Botrytis cinerea by the climatic conditions, soil circumstances and grape-varietes of Tokaj-region. As the result of noble rot, aszú-berries represent the most important raw material of the world-wide known Tokaji Aszú Wine. The objective of this study was to measure the penicillin-content of aszú-berries and Tokaj Wine Specialities. As the result of infection of Botrytis free way can be opened in front of other mould strains, especially Penicillium and Aspergillus. There is a possibility to the presence of Penicillium chrysogenum on the surface of aszú-berries during noble rotting process. P. chrysogenum was found to produce the so called Naturally Derived Penicillins such as Penicillin-V (phenoximethyl-penicillin).
According to our findings the Penicillin-V content was detected between 0.4 and 26 mg/1 wine and 0-74 mg/kg aszú-berry. These values do net have therapeutic effect, but physiological benefit and it can be a basis to the qualification of the raw material of Tokaj Wine Specialities naturally together with the other component of noble rotted berries and botrytized wines.
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Fire blight in Hungary between 1996 and 2003
67-70.Views:121Shoot blight of pome fruits caused by Erwinia amylovora, i.e. fire blight, is present in numerous countries of Europe. The disease must have entered into Hungary in the middle of the 1990's and it was first noted and, respectively, identified in 1996 (Bacs-Kiskun county). The losses caused by the pathogen appeared — in orchards and scattered sites of production — in four counties, namely Bacs-Kiskun, Baranya, Bekes and Csongrad at the beginning. From June 1996, a process of eliminating infected parts started in the course of a large action performed under the control of the Department of Plant Protection and Agro-Environmental Economy of the Ministry of Agriculture, under the direction of the plant protection inspectors of the then existing Stations of Plant Health and Soil Conservation. The 'operation' against the disease commenced by cutting back out the infected parts of the canopy and, grubbing them out, respectively. As for the spread of the pathogen (1996-1998) it could be observed that the disease entered into Hungary from the south, south-east and then it also spread into the middle part of the country. As a result of adequate official action and efforts as well as of adequate chemical and antibiotic treatments, moreover because of the introduction of more modern technologies of plant cultivation and those of plant protection it can be reported on that the pathogen hardly appears or does not occur at all on the northern, north-western part of the country. The infection also appears mainly on the parts east of the Danube. Cultivars less susceptible or non-susceptible to the disease are planted in recently established orchards what is also a considerable factor in respect of preventing spread of the pathogen.
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Comparison of three soil cultivation method in case of grape
47-50.Views:204Our results show that the different cultivation methods have effect on the yield and the grape quality as well. In 2008 and 2009 the straw mulch proved to be the best solution regarding the yield. The soil compaction was reduced in these plots and the photosintetical activity was also higher. Straw mulch could conserve the moisture content of the soil. The less yield was observed in case of barely covered vines and also the vegetative growth of the grape was less intensive in case of these vines, but it seems to be contributed to the conformation of suitable microclimate for Botrytis infection.
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Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation
53-56.Views:159The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.